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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
23/11/2022 |
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Data da última atualização: |
24/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROMERO PELÁEZ, R. D.; WISCHRAL, D.; CUNHA, J. R. B.; MENDES, T. D.; PACHECO, T. F.; SIQUEIRA, F. G. de; ALMEIDA, J. R. M. de. |
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Afiliação: |
RUBÉN DARÍO ROMERO PELÁEZ, UNIVERSIDADE DE BRASÍLIA; DAIANA WISCHRAL, CNPAE; JOICE RAÍSA BARBOSA CUNHA, CNPAE; THAIS DEMARCHI MENDES, CNPAE; THALYTA FRAGA PACHECO, CNPAE; FELIX GONCALVES DE SIQUEIRA, CNPAE; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE. |
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Título: |
Production of enzymatic extract with high cellulolytic and oxidative activities by co-culture of Trichoderma reesei and Panus lecomtei. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Fermentation, v. 8, n. 10, 522, 2022. |
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Páginas: |
17 p. |
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ISSN: |
2311-5637 |
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DOI: |
https://doi.org/10.3390/fermentation8100522 |
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Idioma: |
Inglês |
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Conteúdo: |
This work aimed to produce enzymatic fungi extracts with hydrolytic and oxidative activities to hydrolyze lignocellulosic biomasses efficiently. For this, the fungi Trichoderma reesei and Panus lecomtei were co-cultured using the vegetable biomasses oil palm decanter cake, wheat bran, and cottonseed cake as substrates in submerged fermentation. T. reesei and P. lecomtei showed partially compatible positive interaction on plates. The co-cultures respond positively to variations of temperature and inoculum interval, generating extracts responsible for higher hydrolysis yield when grown at 25 °C, and P. lecomtei is inoculated 24 h after T. reesei. The enzymatic extract production of co-cultures was also improved by modifying the components of the initial media and evaluating enzymatic activities, hydrolysis of sugarcane bagasse pretreated by autohydrolysis and ethanol production as a response. Five culture media were evaluated with variations in the composition of nutritional elements, minerals and substrates. The best extract showed a maximum cellulose hydrolysis efficiency of 68.7% compared with 44.8% of the initial medium. The ethanolic fermentation of hydrolysates obtained by co-culture extracts showed higher ethanol yields than monocultures. This work demonstrates the use of fungi co-cultures to produce enzymatic extracts composed of cellulolytic, hemicellulolytic, and ligninolytic enzymes complexes, which allow hydrolyzing pretreated lignocellulosic biomass with high efficiency, generating hydrolysates that are easier fermented by yeast. MenosThis work aimed to produce enzymatic fungi extracts with hydrolytic and oxidative activities to hydrolyze lignocellulosic biomasses efficiently. For this, the fungi Trichoderma reesei and Panus lecomtei were co-cultured using the vegetable biomasses oil palm decanter cake, wheat bran, and cottonseed cake as substrates in submerged fermentation. T. reesei and P. lecomtei showed partially compatible positive interaction on plates. The co-cultures respond positively to variations of temperature and inoculum interval, generating extracts responsible for higher hydrolysis yield when grown at 25 °C, and P. lecomtei is inoculated 24 h after T. reesei. The enzymatic extract production of co-cultures was also improved by modifying the components of the initial media and evaluating enzymatic activities, hydrolysis of sugarcane bagasse pretreated by autohydrolysis and ethanol production as a response. Five culture media were evaluated with variations in the composition of nutritional elements, minerals and substrates. The best extract showed a maximum cellulose hydrolysis efficiency of 68.7% compared with 44.8% of the initial medium. The ethanolic fermentation of hydrolysates obtained by co-culture extracts showed higher ethanol yields than monocultures. This work demonstrates the use of fungi co-cultures to produce enzymatic extracts composed of cellulolytic, hemicellulolytic, and ligninolytic enzymes complexes, which allow hydrolyzing pretreated lignocellulosic biomass with high effi... Mostrar Tudo |
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Palavras-Chave: |
Co-cultures; Ethanolic fermentation; Laccases; Panus lecomtei. |
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Thesaurus Nal: |
Cellulases; Trichoderma reesei. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148635/1/Production-of-enzymatic-extract.pdf
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Marc: |
LEADER 02486naa a2200301 a 4500
001 2148635
005 2022-11-24
008 2022 bl uuuu u00u1 u #d
022 $a2311-5637
024 7 $ahttps://doi.org/10.3390/fermentation8100522$2DOI
100 1 $aROMERO PELÁEZ, R. D.
245 $aProduction of enzymatic extract with high cellulolytic and oxidative activities by co-culture of Trichoderma reesei and Panus lecomtei.$h[electronic resource]
260 $c2022
300 $a17 p.
520 $aThis work aimed to produce enzymatic fungi extracts with hydrolytic and oxidative activities to hydrolyze lignocellulosic biomasses efficiently. For this, the fungi Trichoderma reesei and Panus lecomtei were co-cultured using the vegetable biomasses oil palm decanter cake, wheat bran, and cottonseed cake as substrates in submerged fermentation. T. reesei and P. lecomtei showed partially compatible positive interaction on plates. The co-cultures respond positively to variations of temperature and inoculum interval, generating extracts responsible for higher hydrolysis yield when grown at 25 °C, and P. lecomtei is inoculated 24 h after T. reesei. The enzymatic extract production of co-cultures was also improved by modifying the components of the initial media and evaluating enzymatic activities, hydrolysis of sugarcane bagasse pretreated by autohydrolysis and ethanol production as a response. Five culture media were evaluated with variations in the composition of nutritional elements, minerals and substrates. The best extract showed a maximum cellulose hydrolysis efficiency of 68.7% compared with 44.8% of the initial medium. The ethanolic fermentation of hydrolysates obtained by co-culture extracts showed higher ethanol yields than monocultures. This work demonstrates the use of fungi co-cultures to produce enzymatic extracts composed of cellulolytic, hemicellulolytic, and ligninolytic enzymes complexes, which allow hydrolyzing pretreated lignocellulosic biomass with high efficiency, generating hydrolysates that are easier fermented by yeast.
650 $aCellulases
650 $aTrichoderma reesei
653 $aCo-cultures
653 $aEthanolic fermentation
653 $aLaccases
653 $aPanus lecomtei
700 1 $aWISCHRAL, D.
700 1 $aCUNHA, J. R. B.
700 1 $aMENDES, T. D.
700 1 $aPACHECO, T. F.
700 1 $aSIQUEIRA, F. G. de
700 1 $aALMEIDA, J. R. M. de
773 $tFermentation$gv. 8, n. 10, 522, 2022.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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| 3. |  | LEIVAS, J. F.; TEIXEIRA, A. H. de C.; SILVA, G. B. S. da. Aplicação de modelo agrometeorológico espectral no perímetro irrigado da bacia do São Francisco, utilizando imagens RAPIDEYE. In: SIMPÓSIO DA BACIA HIDROGRÁFICA DO RIO SÃO FRANCISCO, 1., 2016, Juazeiro, BA; Petrolina, PE. Anais... Juazeiro; Petrolina: CBHSF, 2016. 7 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Territorial. |
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| 7. | | SILVA, G. B. S. da; FORMAGGIO, A. R.; SHIMABUKURO, Y. E.; ADAMI, M.; SANO, E. E. Discriminação da cobertura vegetal do Cerrado matogrossense por meio de imagens MODIS. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 45, n. 2, p. 186-194, fev. 2010 Título em inglês: Discrimination of Cerrado vegetation cover in the state of Mato Grosso using MODIS images.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 11. | | TAKEMURA, C. M.; SILVA, G. B. S. da; OLIVEIRA, S. R. de M.; MOURA, M. F. Desambiguação de topônimos usando dicionários geográficos. In: SEMINÁRIO DA REDE AGROHIDRO, 4., 2016, Brasília, DF. Água e agricultura: incertezas e desafios para a sustentabilidade frente às mudanças do clima e do uso da terra: anais. Planaltina, DF: Embrapa Cerrados, 2016. p. 67-74.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 12. | | TAKEMURA, C. M.; SILVA, G. B. S. da; OLIVEIRA, S. R. de M.; MOURA, M. F. Desambiguação de topônimos usando dicionários geográficos. In: SEMINÁRIO DA REDE AGROHIDRO, 4., 2016, Brasília, DF. Água e agricultura: incertezas e desafios para a sustentabilidade frente às mudanças do clima e do uso da terra: anais. Planaltina, DF: Embrapa Cerrados, 2016. 290 p. Editores técnicos: Lineu Neiva Rodrigues; Maria Fernanda Moura; Raimundo Cosme de Oliveira Júnior. Disponível em: . Acesso em: 23 dez. 2016.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Territorial. |
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| 15. | | TRABAQUINI, K.; SILVA, G. B. S. da; FORMAGGIO, A. R.; SHIMABUKURO, Y. E.; GALVÃO, L. S. Dynamics and distribution of anthropic occupation in the Cerrado of Mato Grosso in the period from 1990 to 2008. Geografia, Rio Claro, v. 38, n. 2, p. 209-224, maio/ago. 2013. p. 209-224| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Territorial. |
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| Registros recuperados : 180 | |
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