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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
31/08/1993 |
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Data da última atualização: |
31/08/1993 |
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Autoria: |
ROCO, A.; CASTANEDA, P.; PEREZ, L. M. |
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Afiliação: |
Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Quimicas y Farmaceuticas, Universidad de Chile, Casilla 233, Santiago 1, Chile. |
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Título: |
Oligosaccharides releaded by pectinase treament of Citrus limon seedlings are elictores of the plant response. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
Phytochemistry, v.33, n.6, p.1301-1306, 1993. |
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ISSN: |
0031-9422 |
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Idioma: |
Inglês |
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Conteúdo: |
Oligosaccharides of different sizes were released from intact Citrus limon seedlings treated with endopolygalacturonase obtained from Alternaria alternata. Also, an increaes of phenylalanine ammonia lyase (PAL) acitivity and the formation of a phytoalexin, were observed. Only mechanically damaged, but not intact seedlings, were able to increase their PAL activity and to synthesize a phytoalexin, in response to isolated oligomes released from plant cell walls or obtained from polygalacturonic acid (PGA). The active oligomes (pectic fragments obtained from cell walls or pectic fragments obtained from PGA) contained between 17 and 23, or 13 to 20 units of galacturonic acid, respectively. Maximal PAL activation was obtained after 20 hr treatment of intact seedlings with endopolygalacturonase and after 7 or 4 hr of treatmrnt of damaged seedlings with endopolygalacturonase or oligosaccharides, respectively. Aloso, as a result of the increase of PAL activity, th appearance of a phytoalexin was observed, always after 42 hr of PAL activation. Immunoprecipitation with PAL antibodies confirmed that PAL activation was due to an increase in the amount of enzyme. |
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Palavras-Chave: |
Oligonasaccharides; pectinase. |
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Thesagro: |
Alternaria Alternata; Rutaceae. |
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Thesaurus Nal: |
Citrus limon; elicitors. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01804naa a2200229 a 4500
001 1632734
005 1993-08-31
008 1993 bl uuuu u00u1 u #d
022 $a0031-9422
100 1 $aROCO, A.
245 $aOligosaccharides releaded by pectinase treament of Citrus limon seedlings are elictores of the plant response.
260 $c1993
520 $aOligosaccharides of different sizes were released from intact Citrus limon seedlings treated with endopolygalacturonase obtained from Alternaria alternata. Also, an increaes of phenylalanine ammonia lyase (PAL) acitivity and the formation of a phytoalexin, were observed. Only mechanically damaged, but not intact seedlings, were able to increase their PAL activity and to synthesize a phytoalexin, in response to isolated oligomes released from plant cell walls or obtained from polygalacturonic acid (PGA). The active oligomes (pectic fragments obtained from cell walls or pectic fragments obtained from PGA) contained between 17 and 23, or 13 to 20 units of galacturonic acid, respectively. Maximal PAL activation was obtained after 20 hr treatment of intact seedlings with endopolygalacturonase and after 7 or 4 hr of treatmrnt of damaged seedlings with endopolygalacturonase or oligosaccharides, respectively. Aloso, as a result of the increase of PAL activity, th appearance of a phytoalexin was observed, always after 42 hr of PAL activation. Immunoprecipitation with PAL antibodies confirmed that PAL activation was due to an increase in the amount of enzyme.
650 $aCitrus limon
650 $aelicitors
650 $aAlternaria Alternata
650 $aRutaceae
653 $aOligonasaccharides
653 $apectinase
700 1 $aCASTANEDA, P.
700 1 $aPEREZ, L. M.
773 $tPhytochemistry$gv.33, n.6, p.1301-1306, 1993.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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