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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
31/05/2016 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
FARIAS, T. H. V.; PEREIRA, N. L.; PÁDUA, S. B. de; ALVES, L. de O.; SAKABE, R.; BELO, M. A. de A.; PILARSKI, F. |
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Afiliação: |
THAÍS HELOISA VAZ FARIAS, CAUnesp; NYCOLAS LEVY PEREIRA, CAUnesp; SANTIAGO BENITES DE PÁDUA, AquiVet Saúde Aquática; LINDOMAR DE OLIVEIRA ALVES, CAUnesp; ROBERSON SAKABE, UFF; MARCO ANTONIO DE ANDRADE BELO, UNESP; FABIANA PILARSKI, CAUnesp. |
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Título: |
Na2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicus. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 5, p. 431-435, maio. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
The present study aimed to evaluate the effects of Na heparin and Na2EDTA on blood of Piaractus mesopotamicus (360.7±42.4g, 26.4±1.0cm). Twenty fishes were sampled in two experiment trials, ten for erythrocyte fragility analysis and ten for hematologic and plasma biochemical study. The blood collected by venous-caudal puncture was fractioned and stored in anticoagulants solution: Na2EDTA 10%, Na2EDTA 3%, Na heparin 5000 IU and Na heparin 100 IU. Plasmatic levels of calcium presented in the Na2EDTA stored samples were about 80% lower than both heparin groups. Blood samples of P. mesopotamicus stored with Na2EDTA demonstrated increase in the hematocrit and MCV, and decrease in MCHC. The dose-response effect was observed in this study. The results are reinforced by the higher levels of plasmatic protein and hemolysis presented in the Na2EDTA 10% stored blood, confirming the deleterious effect of this anticoagulant treatment on the quality of blood samples. Na2EDTA is not indicated to store P. mesopotamicus blood samples, but sodium heparin at 100 IU is the most recommended anticoagulant, since this treatment presented the lower rate of alterations in the stored blood. |
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Palavras-Chave: |
EDTA; Fish hematology; Hematologia de peixe; Pacus. |
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Thesagro: |
Coagulação Sangüínea; Heparina. |
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Thesaurus Nal: |
Blood coagulation; EDTA (chelating agent); Heparin; Piaractus mesopotamicus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/143607/1/Na2EDTA-anticoagulant.pdf
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Marc: |
LEADER 02085naa a2200313 a 4500
001 2045983
005 2017-12-29
008 2016 bl uuuu u00u1 u #d
100 1 $aFARIAS, T. H. V.
245 $aNa2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicus.
260 $c2016
520 $aThe present study aimed to evaluate the effects of Na heparin and Na2EDTA on blood of Piaractus mesopotamicus (360.7±42.4g, 26.4±1.0cm). Twenty fishes were sampled in two experiment trials, ten for erythrocyte fragility analysis and ten for hematologic and plasma biochemical study. The blood collected by venous-caudal puncture was fractioned and stored in anticoagulants solution: Na2EDTA 10%, Na2EDTA 3%, Na heparin 5000 IU and Na heparin 100 IU. Plasmatic levels of calcium presented in the Na2EDTA stored samples were about 80% lower than both heparin groups. Blood samples of P. mesopotamicus stored with Na2EDTA demonstrated increase in the hematocrit and MCV, and decrease in MCHC. The dose-response effect was observed in this study. The results are reinforced by the higher levels of plasmatic protein and hemolysis presented in the Na2EDTA 10% stored blood, confirming the deleterious effect of this anticoagulant treatment on the quality of blood samples. Na2EDTA is not indicated to store P. mesopotamicus blood samples, but sodium heparin at 100 IU is the most recommended anticoagulant, since this treatment presented the lower rate of alterations in the stored blood.
650 $aBlood coagulation
650 $aEDTA (chelating agent)
650 $aHeparin
650 $aPiaractus mesopotamicus
650 $aCoagulação Sangüínea
650 $aHeparina
653 $aEDTA
653 $aFish hematology
653 $aHematologia de peixe
653 $aPacus
700 1 $aPEREIRA, N. L.
700 1 $aPÁDUA, S. B. de
700 1 $aALVES, L. de O.
700 1 $aSAKABE, R.
700 1 $aBELO, M. A. de A.
700 1 $aPILARSKI, F.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 5, p. 431-435, maio. 2016.
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Embrapa Unidades Centrais (AI-SEDE) |
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