Data/hora: 05/06/2024 06:27:29

Biblioteca(s): 	Embrapa Agroenergia.	
Data corrente: 	05/07/2017	
Data da última atualização: 	13/09/2017	
Autoria: 	COSATE, M. R. V.; SAKAMOTO, T.; MENDES, T. A. de O.; MOREIRA, E. C.; SILVA, C. G. R. da; BRASIL, B. dos S. A. F.; OLIVEIRA, C. S. F.; AZEVEDO, V. A. de; ORTEGA, J. M.; LEITE, R. C.; HADDAD, J. P.	
Afiliação: 	MARIA RAQUEL V. COSATE; TETSU SAKAMOTO; TIAGO ANTÔNIO DE OLIVEIRA MENDES; ÉLVIO C. MOREIRA; CARLOS G. REGIS DA SILVA; BRUNO DOS SANTOS ALVES FIGUEIREDO B, CNPAE; CAMILA S. F. OLIVEIRA; VASCO ARISTON DE AZEVEDO; JOSÉ MIGUEL ORTEGA; RÔMULO C. LEITE; JOÃO PAULO HADDAD.	
Título: 	Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock.	
Ano de publicação: 	2017	
Fonte/Imprenta: 	BMC Veterinary Research, v. 13, n. 1, p. 177, 2017.	
DOI: 	10.1186/s12917-017-1081-9	
Idioma: 	Inglês	
Conteúdo: 	Background: Leptospirosis is caused by pathogenic spirochetes of the genus Leptospira spp. This zoonotic disease is distributed globally and affects domestic animals, including cattle. Leptospira interrogans serogroup Sejroe serovar Hardjo and Leptospira borgpetersenii serogroup Sejroe serovar Hardjo remain important species associated with this reproductive disease in livestock production. Previous studies on Brazilian livestock have reported that L. interrogans serovar Hardjo is the most prevalent leptospiral agent in this country and is related to clinical signs of leptospirosis, which lead to economic losses in production. Here, we described the isolation of three clinical strains (Norma, Lagoa and Bolivia) obtained from leptospirosis outbreaks that occurred in Minas Gerais state in 1994 and 2008. Results: Serological and molecular typing using housekeeping (secY and 16SrRNA) and rfb locus (ORF22 and ORF36) genes were applied for the identification and comparative analysis of Leptospira spp. Our results identified the three isolates as L. interrogans serogroup Sejroe serovar Hardjo and confirmed the occurrence of this bacterial strain in Brazilian livestock. Genetic analysis using ORF22 and ORF36 grouped the Leptospira into serogroup Sejroe and subtype Hardjoprajitno. Genetic approaches were also applied to compare distinct serovars of L. interrogans strains by verifying the copy numbers of the IS1500 and IS1533 insertion sequences (ISs). The IS1500 copy number varied among the analyzed L. interrogans strains. Conclusion: This study provides evidence that L. interrogans serogroup Sejroe serovar Hardjo subtype Hardjoprajitno causes bovine leptospirosis in Brazilian production. The molecular results suggested that rfb locus (ORF22 and ORF36) could improve epidemiological studies by allowing the identification of Leptospira spp. at the serogroup level. Additionally, the IS1500 and IS1533 IS copy number analysis suggested distinct genomic features among closely related leptospiral strains.	
Palavras-Chave: 	Copy number variation; Hardjoprajitno; Insertion sequences; Leptospirosis outbreaks.	
Thesagro: 	Leptospirose.	
Thesaurus Nal: 	cattle.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Agroenergia (CNPAE)	
Exemplares: 	CNPAE - (3144-1) - UPC - AP - DD -  -  -  - DIS