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| Registros recuperados : 18 | |
| 8. |  | BAJJALIEH, N.; ORF, J. H.; HYMOWITZ, I.; JENSEN, A. H. Response of young ckicks to raw, defatted, kunitz trypsin inhibitor variant soybeans as sources of dietary protein. Poultry Science, College Station, v. 59, p. 328-332, 1980.| Biblioteca(s): Embrapa Trigo. |
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| 16. | | NELSON, R. L.; AMDOR, P. J.; ORF, J. H.; LAMBERT, J. W.; CAVINS, J. F.; KLEIMAN, R.; LAVIOLETTE, F. A.; ATHOW, K. L. Evaluation of the USDA soybean germplasm collection: maturity groups 000 to IV (PI 273.483 to PI 427.107). [S.l.]: USDA. Agricultural Research Service, 1987. 267 p. (USDA-ARS. Technical Bulletin, 1718).| Biblioteca(s): Embrapa Trigo. |
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| 17. | | RANDALL, L. N.; AMDOR, P. J.; ORF, J. H.; LAMBERT, J. W.; CAUINS, J. F.; KLEIMAN, R.; LAVIOLETT, F. A.; ATHOW, K. L. Evaluation of the USDA soybean germplasm collection: maturity groups 000 to IV (PI 273.483 to PI 427.107). [S.l.]: U.S. Department of Agriculture. Agricultural Research Service, 1987. 267p. (USDA. Technical Bulletin, 1718).| Biblioteca(s): Embrapa Soja. |
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| 18. | | LUESCHEN, W. E.; FORD, J. H.; EVANS, S. D.; KANNE, B. K.; HOVERSTAD, T. R.; RANDALL, G. W.; ORF, J. H.; HICKS, D. R. Tillage, row spacing, and planting date effects on soybean following corn or wheat. Journal of Production Agriculture, v.5, n.2, p.254-260, 1992.| Biblioteca(s): Embrapa Soja. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Unidades Centrais. |
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Data corrente: |
22/01/2024 |
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Data da última atualização: |
02/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
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Autoria: |
MAGARELLI, G.; SILVA, J. G. da; RIBEIRO, C. L.; FREITAS, T. V. de; RODRIGUES, M. A.; GIL, E. de S.; MARRACCINI, P.; SOUZA, J. R. de; CASTRO, C. S. P. de; BEMQUERER, M. P. |
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Afiliação: |
GABRIELLA MAGARELLI, Cenargen; JONATAS GOMES DA SILVA, UNIVERSIDADE FEDERAL DO OESTE DA BAHIA; CAROLINE LUCHTENBERG RIBEIRO; THIAGO VIANA DE FREITAS; MAGALI APARECIDA RODRIGUES, UNIVERSIDADE DE SAO PAULO; ERIC DE SOUZA GIL, UNIVERSIDADE FEDERAL DE GOIAS; PIERRE MARRACCINI, CIRAD; JURANDIR RODRIGUES DE SOUZA, UNIVERSIDADE DE BRASÍLIA; CLARISSA SILVA PIRES DE CASTRO, GIS; MARCELO PORTO BEMQUERER, CNPGL. |
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Título: |
A voltammetric peptide biosensor for Cu2+ metal ion quantification in coffee seeds. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Journal of Inorganic Biochemistry, v. 251, 112441, 2024. |
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DOI: |
https://doi.org/10.1016/j.jinorgbio.2023.112441 |
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Idioma: |
Inglês |
|
Conteúdo: |
A prion-derived copper(II)-binding peptide was assembled onto a gold electrode for the building of a voltammetric biosensor for measuring the Cu2+ metal ion in biological samples. The chosen sequence was HCVNITKQHTVTTTT-NH2, with an appended cysteine residue for binding to the gold surface as a self-assembled monolayer and a histidine residue as the anchorage point for copper(II) complexation. The biosensor showed a linear range of 10-7 to 10-6 M with an 8.0 x 10-8 M detection limit and a 1.0 x 10-7 M quantification limit, with good precision, trueness, and absence of matrix effect. The quantification of Cu2+ was performed in the presence of other transition metal ions, such as Zn2+, Cd2+, Fe2+, or Ni2+, which indicates the excellent selectivity of the biosensor. When the modified electrode was applied for measuring copper(II) in calcined coffee seeds, a difference in copper amount was observed between two Coffea arabica cultivars that were submitted to a treatment with a copper-based antifungal, showing the applicability of the biosensor in the agricultural field. |
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Palavras-Chave: |
Biossensor peptídico; Coffee seeds; Peptide biosensor; Prion peptide; Voltametria; Voltammetry. |
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Thesagro: |
Café; Peptídeo; Semente. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
|
Marc: |
LEADER 02087naa a2200349 a 4500
001 2161562
005 2024-02-02
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.jinorgbio.2023.112441$2DOI
100 1 $aMAGARELLI, G.
245 $aA voltammetric peptide biosensor for Cu2+ metal ion quantification in coffee seeds.$h[electronic resource]
260 $c2024
520 $aA prion-derived copper(II)-binding peptide was assembled onto a gold electrode for the building of a voltammetric biosensor for measuring the Cu2+ metal ion in biological samples. The chosen sequence was HCVNITKQHTVTTTT-NH2, with an appended cysteine residue for binding to the gold surface as a self-assembled monolayer and a histidine residue as the anchorage point for copper(II) complexation. The biosensor showed a linear range of 10-7 to 10-6 M with an 8.0 x 10-8 M detection limit and a 1.0 x 10-7 M quantification limit, with good precision, trueness, and absence of matrix effect. The quantification of Cu2+ was performed in the presence of other transition metal ions, such as Zn2+, Cd2+, Fe2+, or Ni2+, which indicates the excellent selectivity of the biosensor. When the modified electrode was applied for measuring copper(II) in calcined coffee seeds, a difference in copper amount was observed between two Coffea arabica cultivars that were submitted to a treatment with a copper-based antifungal, showing the applicability of the biosensor in the agricultural field.
650 $aCafé
650 $aPeptídeo
650 $aSemente
653 $aBiossensor peptídico
653 $aCoffee seeds
653 $aPeptide biosensor
653 $aPrion peptide
653 $aVoltametria
653 $aVoltammetry
700 1 $aSILVA, J. G. da
700 1 $aRIBEIRO, C. L.
700 1 $aFREITAS, T. V. de
700 1 $aRODRIGUES, M. A.
700 1 $aGIL, E. de S.
700 1 $aMARRACCINI, P.
700 1 $aSOUZA, J. R. de
700 1 $aCASTRO, C. S. P. de
700 1 $aBEMQUERER, M. P.
773 $tJournal of Inorganic Biochemistry$gv. 251, 112441, 2024.
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Embrapa Unidades Centrais (AI-SEDE) |
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