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Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
01/07/2004 |
Data da última atualização: |
26/07/2017 |
Autoria: |
PINEDO, F. J. R.; MOSCARDI, F.; LUQUE, T.; OLSZEWSKI, J. A.; RIBEIRO, B. M. |
Título: |
Inactivation of the ecdysteroid UDP-glucosyltransferase (egt) gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) improves its virulence towards its insect host. |
Ano de publicação: |
2003 |
Fonte/Imprenta: |
Biological Control, v. 27, n. 3, p. 336-344, July 2003. |
Idioma: |
Inglês |
Conteúdo: |
Some baculovirus have been genetically modified for the inactivation of their ecdysteroid
glucosyltransferase (egt) gene, and these viruses were shown to kill infected larvae more rapidly when compared to wild-type virus infections. We have previously identified, cloned, and sequenced the egt gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV). Here we present data regarding the construction of an egt minus (egt?x2212;) AgMNPV and its virulence towards its insect host. We have inserted an hsp70-lacZ (3.7 kb) gene cassette into the egt gene open reading frame (ORF) and purified a recombinant AgMNPV (vAgEGT?x394;-lacZ). Bioassays with third-instar A. gemmatalis larvae showed that viral occlusion body (OB) production were consistently lower from infections with vAgEGT?x394;-lacZ compared to the wild-type virus. A mean of 20.4Ã?108 OBs/g/larva and 40.7Ã?108 OBs/g/larva was produced from vAgEGT?x394;-lacZ and AgMNPV infections, respectively. The mean lethal concentration which killed 50% of insects in a treatment group (LC50) for the 10th day after virus treatment (DAT) was 3.9-fold higher for the wild-type virus compared to vAgEGT?x394;-lacZ. The recombinant virus killed A. gemmatalis larvae significantly faster (ca. 1?x2013;2.8 days), than the wild-type
AgMNPV. Therefore, the vAgEGT?x394;-lacZ was more efficacious for the control of A.
gemmatalis larvae (in bioassays) compared to wild-type AgMNPV. |
Palavras-Chave: |
Ecdysteroid glucosyltransferase; Velvetbean caterpillar. |
Thesagro: |
Anticarsia Gemmatalis; Baculovirus; Controle Biológico; Engenharia Genética. |
Categoria do assunto: |
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Marc: |
LEADER 02224naa a2200241 a 4500 001 1467192 005 2017-07-26 008 2003 bl --- 0-- u #d 100 1 $aPINEDO, F. J. R. 245 $aInactivation of the ecdysteroid UDP-glucosyltransferase (egt) gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) improves its virulence towards its insect host. 260 $c2003 520 $aSome baculovirus have been genetically modified for the inactivation of their ecdysteroid glucosyltransferase (egt) gene, and these viruses were shown to kill infected larvae more rapidly when compared to wild-type virus infections. We have previously identified, cloned, and sequenced the egt gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV). Here we present data regarding the construction of an egt minus (egt?x2212;) AgMNPV and its virulence towards its insect host. We have inserted an hsp70-lacZ (3.7 kb) gene cassette into the egt gene open reading frame (ORF) and purified a recombinant AgMNPV (vAgEGT?x394;-lacZ). Bioassays with third-instar A. gemmatalis larvae showed that viral occlusion body (OB) production were consistently lower from infections with vAgEGT?x394;-lacZ compared to the wild-type virus. A mean of 20.4Ã?108 OBs/g/larva and 40.7Ã?108 OBs/g/larva was produced from vAgEGT?x394;-lacZ and AgMNPV infections, respectively. The mean lethal concentration which killed 50% of insects in a treatment group (LC50) for the 10th day after virus treatment (DAT) was 3.9-fold higher for the wild-type virus compared to vAgEGT?x394;-lacZ. The recombinant virus killed A. gemmatalis larvae significantly faster (ca. 1?x2013;2.8 days), than the wild-type AgMNPV. Therefore, the vAgEGT?x394;-lacZ was more efficacious for the control of A. gemmatalis larvae (in bioassays) compared to wild-type AgMNPV. 650 $aAnticarsia Gemmatalis 650 $aBaculovirus 650 $aControle Biológico 650 $aEngenharia Genética 653 $aEcdysteroid glucosyltransferase 653 $aVelvetbean caterpillar 700 1 $aMOSCARDI, F. 700 1 $aLUQUE, T. 700 1 $aOLSZEWSKI, J. A. 700 1 $aRIBEIRO, B. M. 773 $tBiological Control$gv. 27, n. 3, p. 336-344, July 2003.
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
25/03/2024 |
Data da última atualização: |
25/03/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
C - 0 |
Autoria: |
HALLERMAN, E.; BREDLAU, J.; CAMARGO, L. S. de A.; DAGLI, M. L. Z.; KAREMBU, M.; KOVICH, D.; MUIA, A. N.; MURRONE, M. L.; ROCHA‑SALAVARRIETA, P. J.; ROMERO‑ALDEMITA, R.; TIZARD, M.; WALTON, M.; WRAY‑CAHEN, D. |
Afiliação: |
ERIC HALLERMAN, VIRGINIA POLYTECHNIC INSTITUTE AND STATE UNIVERSITY; JUSTIN BREDLAU, U.S. DEPARTMENT OF AGRICULTURE; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; MARIA LUCIA ZAIDAN DAGLI, UNIVERSIDADE DE SAO PAULO; MARGARET KAREMBU, INTERNATIONAL SERVICE FOR THE ACQUISITION OF AGRI-BIOTECH APPLICATIONS AFRICENTER; DANIEL KOVICH, U.S. DEPARTMENT OF AGRICULTURE; ANNE NDANU MUIA, NATIONAL BIOSAFETY AUTHORITY, NAIROBI; MARIANA L. MURRONE, MINISTRY OF AGRICULTURE, ARGENTINA; PEDRO JESUS ROCHA‑SALAVARRIETA, INTER-AMERICAN INSTITUTE FOR COOPERATION ON AGRICULTURE, SAN JOSE; RHODORA ROMERO‑ALDEMITA, INTERNATIONAL SERVICE FOR THE ACQUISITION OF AGRI-BIOTECH APPLICATIONS; MARK TIZARD, COMMON WEALTH SCIENTIFC AND INDUSTRIAL RESEARCH ORGANISATION, GEELONG; MARK WALTON, AQUA BOUNTY; DIANE WRAY‑CAHEN, U.S. DEPARTMENT OF AGRICULTURE. |
Título: |
Enabling regulatory policy globally will promote realization of the potential of animal biotechnology. |
Ano de publicação: |
2024 |
Fonte/Imprenta: |
CABI Agriculture and Bioscience, v. 5, n. 1, article 25, 2024. |
DOI: |
https://doi.org/10.1186/s43170-024-00221-6 |
Idioma: |
Inglês |
Conteúdo: |
Animal biotechnologies have the potential to improve the sustainability and security of our global food systems. Government regulatory authorities are responsible for ensuring the safety of food their citizens consume, whether it is produced via conventional breeding methods or biotechnologies. While some countries have implemented animal biotechnology oversight policies, many countries have yet to develop theirs. Historically, regulatory approvals were required before products of biotechnology could enter the marketplace, and the high cost of the approval process limited the number and types of animal and plant products that sought approval. Only one biotech animal in the world that was developed for food production has reached the market under a GMO or rDNA approval process. The advent of genome editing techniques has revolutionized the scientific approach to introducing changes into DNA sequences and how biotechnology can be used to enhance agricultural breeding. Regulatory dialogs about biotechnology also have changed as a result of these new technologies. Regulatory agencies have begun to respond to these scientific advances, and a growing number of countries are looking to modernize regulatory approaches for these products, based on risk (or lack thereof) and similarity to organisms that could be produced via conventional breeding methods. Advances in animal biotechnology, especially genome editing, can accelerate the incorporation of valued phenotypes in animals, including enhanced yield, disease resistance, resilience to changing climate, and improved animal welfare, as well as food qualities valued by consumers. For animals with these biotechnology-introduced traits to enter agricultural production and reach consumers, clear risk-proportionate regulatory approaches must be in place, and to facilitate international trade of animal products, regulatory processes need to be aligned and compatible. Effective scientific public communication is crucial to build public trust in precision animal biotechnology and risk-proportionate regulatory approaches. An international workshop on regulatory approaches for animal biotechnology was convened in 2022 with 27 countries represented. We synthesize here technical progress, development of regulatory policy, and strategies for engagement with diverse publics on animal biotechnology reported in the workshop. Our goal is to encourage development and implementation of risk-proportionate regulatory approaches and policies in a global context. MenosAnimal biotechnologies have the potential to improve the sustainability and security of our global food systems. Government regulatory authorities are responsible for ensuring the safety of food their citizens consume, whether it is produced via conventional breeding methods or biotechnologies. While some countries have implemented animal biotechnology oversight policies, many countries have yet to develop theirs. Historically, regulatory approvals were required before products of biotechnology could enter the marketplace, and the high cost of the approval process limited the number and types of animal and plant products that sought approval. Only one biotech animal in the world that was developed for food production has reached the market under a GMO or rDNA approval process. The advent of genome editing techniques has revolutionized the scientific approach to introducing changes into DNA sequences and how biotechnology can be used to enhance agricultural breeding. Regulatory dialogs about biotechnology also have changed as a result of these new technologies. Regulatory agencies have begun to respond to these scientific advances, and a growing number of countries are looking to modernize regulatory approaches for these products, based on risk (or lack thereof) and similarity to organisms that could be produced via conventional breeding methods. Advances in animal biotechnology, especially genome editing, can accelerate the incorporation of valued phenotypes in animals, incl... Mostrar Tudo |
Thesagro: |
Biotecnologia; Bovino; Engenharia Genética; Genoma; Melhoramento Genético Animal. |
Thesaurus NAL: |
Animal breeding; Biotechnology; Genetic engineering. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1163112/1/Enabling-regulatory-policy-globally.pdf
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Marc: |
LEADER 03639naa a2200373 a 4500 001 2163112 005 2024-03-25 008 2024 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1186/s43170-024-00221-6$2DOI 100 1 $aHALLERMAN, E. 245 $aEnabling regulatory policy globally will promote realization of the potential of animal biotechnology.$h[electronic resource] 260 $c2024 520 $aAnimal biotechnologies have the potential to improve the sustainability and security of our global food systems. Government regulatory authorities are responsible for ensuring the safety of food their citizens consume, whether it is produced via conventional breeding methods or biotechnologies. While some countries have implemented animal biotechnology oversight policies, many countries have yet to develop theirs. Historically, regulatory approvals were required before products of biotechnology could enter the marketplace, and the high cost of the approval process limited the number and types of animal and plant products that sought approval. Only one biotech animal in the world that was developed for food production has reached the market under a GMO or rDNA approval process. The advent of genome editing techniques has revolutionized the scientific approach to introducing changes into DNA sequences and how biotechnology can be used to enhance agricultural breeding. Regulatory dialogs about biotechnology also have changed as a result of these new technologies. Regulatory agencies have begun to respond to these scientific advances, and a growing number of countries are looking to modernize regulatory approaches for these products, based on risk (or lack thereof) and similarity to organisms that could be produced via conventional breeding methods. Advances in animal biotechnology, especially genome editing, can accelerate the incorporation of valued phenotypes in animals, including enhanced yield, disease resistance, resilience to changing climate, and improved animal welfare, as well as food qualities valued by consumers. For animals with these biotechnology-introduced traits to enter agricultural production and reach consumers, clear risk-proportionate regulatory approaches must be in place, and to facilitate international trade of animal products, regulatory processes need to be aligned and compatible. Effective scientific public communication is crucial to build public trust in precision animal biotechnology and risk-proportionate regulatory approaches. An international workshop on regulatory approaches for animal biotechnology was convened in 2022 with 27 countries represented. We synthesize here technical progress, development of regulatory policy, and strategies for engagement with diverse publics on animal biotechnology reported in the workshop. Our goal is to encourage development and implementation of risk-proportionate regulatory approaches and policies in a global context. 650 $aAnimal breeding 650 $aBiotechnology 650 $aGenetic engineering 650 $aBiotecnologia 650 $aBovino 650 $aEngenharia Genética 650 $aGenoma 650 $aMelhoramento Genético Animal 700 1 $aBREDLAU, J. 700 1 $aCAMARGO, L. S. de A. 700 1 $aDAGLI, M. L. Z. 700 1 $aKAREMBU, M. 700 1 $aKOVICH, D. 700 1 $aMUIA, A. N. 700 1 $aMURRONE, M. L. 700 1 $aROCHA‑SALAVARRIETA, P. J. 700 1 $aROMERO‑ALDEMITA, R. 700 1 $aTIZARD, M. 700 1 $aWALTON, M. 700 1 $aWRAY‑CAHEN, D. 773 $tCABI Agriculture and Bioscience$gv. 5, n. 1, article 25, 2024.
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