|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
24/04/2021 |
Data da última atualização: |
12/07/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
TÁVORA, F. T. P. K.; BEVITORI, R.; MELLO, R. N. de; CINTRA, M. M. D. F.; OLIVEIRA NETO, O. B.; FONTES, W.; CASTRO, M. S.; SOUSA, M. V.; FRANCO, O. L.; REIS, A. M. dos. |
Afiliação: |
FABIANO T. P. K. TÁVORA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA, MG; ROSANGELA BEVITORI, CNPAF; RAQUEL NEVES DE MELLO, CNPAF; MARIA MONICA DOMINGUES F CINTRA, CNPAF; OSMUNDO B. OLIVEIRA NETO, CENTRO UNIVERSITARIO UNIEURO, Brasília-DF; WAGNER FONTES, UNIVERSIDADE DE BRASÍLIA; MARIANA S. CASTRO, UNIVERSIDADE DE BRASÍLIA; MARCELO V. SOUSA, UNIVERSIDADE DE BRASÍLIA; OCTAVIO L. FRANCO, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ANGELA MEHTA DOS REIS, Cenargen. |
Título: |
Shotgun proteomics coupled to transient-inducible gene silencing reveal rice susceptibility genes as new sources for blast disease resistance. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Journal of Proteomics, v. 241, 104223, June 2021. |
ISSN: |
1874-3919 |
DOI: |
https://doi.org/10.1016/j.jprot.2021.104223 |
Idioma: |
Inglês Português |
Notas: |
Na publicação: Angela Mehta. |
Conteúdo: |
A comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be further explored to build rice-blast resistance. Significance: R gene-mediated disease resistance is race-specific and often not durable in the field. More recently, advancements in new breeding techniques (NBTs) have made plant disease susceptibility genes (S-genes) a new target to build a broad spectrum and more durable resistance, hence an alternative source to R-genes in breeding programs. We successfully coupled shotgun proteomics and gene silencing tools to prospect and validate new rice-bast susceptibility genes that can be further exploited toward a more resolute blast disease resistance. MenosA comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be furth... Mostrar Tudo |
Palavras-Chave: |
Gene ontology; Plant immunity; PTO-based TIGS; RT-qPCR; S-gene; Transient-Induced gene silencing. |
Thesagro: |
Arroz; Brusone; Doença de Planta; Oryza Sativa. |
Thesaurus Nal: |
blast disease; disease resistance; foliar diseases; Magnaporthe oryzae; plant diseases and disorders; proteomics; rice. |
Categoria do assunto: |
-- S Ciências Biológicas |
Marc: |
LEADER 03484naa a2200469 a 4500 001 2131497 005 2021-07-12 008 2021 bl uuuu u00u1 u #d 022 $a1874-3919 024 7 $ahttps://doi.org/10.1016/j.jprot.2021.104223$2DOI 100 1 $aTÁVORA, F. T. P. K. 245 $aShotgun proteomics coupled to transient-inducible gene silencing reveal rice susceptibility genes as new sources for blast disease resistance.$h[electronic resource] 260 $c2021 500 $aNa publicação: Angela Mehta. 520 $aA comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be further explored to build rice-blast resistance. Significance: R gene-mediated disease resistance is race-specific and often not durable in the field. More recently, advancements in new breeding techniques (NBTs) have made plant disease susceptibility genes (S-genes) a new target to build a broad spectrum and more durable resistance, hence an alternative source to R-genes in breeding programs. We successfully coupled shotgun proteomics and gene silencing tools to prospect and validate new rice-bast susceptibility genes that can be further exploited toward a more resolute blast disease resistance. 650 $ablast disease 650 $adisease resistance 650 $afoliar diseases 650 $aMagnaporthe oryzae 650 $aplant diseases and disorders 650 $aproteomics 650 $arice 650 $aArroz 650 $aBrusone 650 $aDoença de Planta 650 $aOryza Sativa 653 $aGene ontology 653 $aPlant immunity 653 $aPTO-based TIGS 653 $aRT-qPCR 653 $aS-gene 653 $aTransient-Induced gene silencing 700 1 $aBEVITORI, R. 700 1 $aMELLO, R. N. de 700 1 $aCINTRA, M. M. D. F. 700 1 $aOLIVEIRA NETO, O. B. 700 1 $aFONTES, W. 700 1 $aCASTRO, M. S. 700 1 $aSOUSA, M. V. 700 1 $aFRANCO, O. L. 700 1 $aREIS, A. M. dos 773 $tJournal of Proteomics$gv. 241, 104223, June 2021.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registros recuperados : 131 | |
121. | | RIBEIRO, T. P.; LOURENCO, I. T.; MELO, B. P. de; MORGANTE, C. V.; SALLES FILHO, A.; LINS, C. B. J.; FERREIRA, G. F.; MELLO, G. N.; MACEDO, L. L. P. de; LUCENA, W. A.; SILVA, M. C. M. da; OLIVEIRA‑NETO, O. B.; SA, M. F. G. de. Improved cotton transformation protocol mediated by Agrobacterium and biolistic combined-methods. Planta, v. 254, 20, 2021.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
| |
122. | | VASCONCELOS, E. A. R.; SANTANA, C. G.; GODOY, C. V.; SEIXAS, C. D. S.; SILVA, M. S.; MOREIRA, L. R. S.; OLIVEIRA-NETO, O. B.; PRICE, D.; FITCHES, E.; FERREIRA FILHO, E. X.; MEHTA, A.; GATEHOUSE, J. A.; GROSSI-DE-SA, M. F. A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination. BMC Biotechnology, v. 11, 2011. Disponível em:Acesso em: 1 mar. 2011.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
| |
123. | | VASCONCELOS, E. A. R.; SANTANA, C. G.; GODOY, C. V.; SEIXAS, C. D. S.; SILVA, M. S.; MOREIRA, L. R. S.; OLIVEIRA-NETO, O. B.; PRICE, D.; FITCHES, E.; FILHO, E. X. F.; REIS, A. M. dos; GATEHOUSE, J. A.; SA, M. F. G. de. A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination. BMC Biotechnology, v. 11, n. 14, 2011.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Cerrados. |
| |
124. | | OLIVEIRA, R. S. de; OLIVEIRA NETO, O. B. de; WANCHAO, N.; COSTA, P. H. A. da; EVANGELISTA, I. B. R.; LEONARDECZ, E.; ROMANO, E.; COUTINHO, M. V.; VIANA, A. A. B.; SILVA, M. C. M. da S.; ROCHA, T. L.; GROSSI DE SÁ, M. F. Transformação de algodoeiro via tubo polínico: otimização e perspectivas de aplicação. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. 30 p. (Embrapa Recursos Genéticos e Biotecnologia. Boletim de pesquisa e desenvolvimento, 184).Tipo: Boletim de Pesquisa e Desenvolvimento |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
125. | | OLIVEIRA, R. S.; OLIVEIRA NETO, O. B.; EVANGELISTA, I. B. R.; COSTA, P. H. A.; PAES, N. S.; MUCHAGATA, I. S.; GURGEL, F. L.; DIAS, S. C.; ROCHA, T. L.; LIMA, L. M.; MATTAR, M. C. S.; GROSSI-DE-SÁ, M. F. Transformação de plantas de algodão (Gossypium hirsutum var. BRS Cedro) resistente a insetos-praga. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 11., 2006, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. p. 94.Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
126. | | OLIVEIRA, R. S. de; OLIVEIRA NETO, O. B.; MOURA, H. F. N.; MACEDO, L. L. P. de; ARRAES, F. B. M.; LUCENA, W. A.; LOURENCO TESSUTTI, I. T.; BARBOSA, A. A. de D.; SILVA, M. C. M. da; GROSSI DE SA, M. F. Transgenic cotton plants expressing Cry1Ia12 toxin confer resistance to fall armyworm (Spodoptera frugiperda) and cotton boll weevil (Anthonomus grandis). Frontiers in Plant Science, v. 7, article 165 , 2016.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
127. | | OLIVEIRA, R. S. de; OLIVEIRA NETO, O. B.; MOURA, H. F. N.; MACEDO, L. L. P. de; ARRAES, F. B M.; LUCENA, W. A.; LOURENCO, I. T.; BARBOSA, A. de D.; SILVA, M. C. M. da; SA, M. F. G. de. Transgenic cotton plants expressing Cry1la12 toxin confer resistance to fall armyworm (Spodoptera frugiperda) and cotton boll weevil (Anthonomus grandis). Frontiers in Plant Science, v. 7, Article 165, 2016.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Algodão. |
| |
128. | | RIBEIRO, T. P.; VASQUEZ, D. D. N.; MACEDO, L. L. P. de; LOURENCO, I. T.; VALENÇA, D. C.; OLIVEIRA-NETO, O. B.; PAES-DE-MELO, B.; RODRIGUES-SILVA, P. L.; FIRMINO, A. A. P.; BASSO, M. F.; LINS, C. B. J.; NEVES, M. R.; MOURA, S. M.; TRIPODE, B. M. D.; MIRANDA, J. E.; SILVA, M. C. M. da; SA, M. F. G. de. Stabilized double-stranded RNA strategy improves cotton resistance to CBW (Anthonomus grandis). International Journal of Molecular Sciences, v. 23, 2022. 13713. Na publicação: Leonardo L. P. Macedo; Isabela T. Lourenço-Tessutti; Maria F. Grossi-de-Sa.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Algodão; Embrapa Recursos Genéticos e Biotecnologia. |
| |
129. | | GROSSI DE SÁ, M. F.; MAGALHÃES, M. Q. de; SILVA, M. S.; SILVA, S. M. B.; DIAS, S. C.; NAKASU, E. Y. T.; BRUNETTA, P. S. F.; OLIVEIRA, G. R.; OLIVEIRA NETO, O. B.; OLIVEIRA, R. S. de; SOARES, L. H. B.; AYUB, M. A. Z.; SIQUEIRA, H. A. A.; FIGUEIRA, E. L. Z. Susceptibility of Anthonomus grandis (Cotton boll weevil) and Spodoptera frugiperda (Fall Armyworm) to a Cry1Ia-type toxin from brazilian Bacillus thuringiensis strain. Journal of Biochemistry and Molecular Biology, v. 40, n. 5, p. 773-782, 2007.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
130. | | GROSSI-DE-SA, M. F.; MAGALHÃES, M. Q. de; SILVA, M. S.; SILVA, S. M. B.; DIAS, S. C.; NAKASU, E. Y. T.; BRUNETTA, P. S. F.; OLIVEIRA, G. R.; OLIVEIRA NETO, O. B. de; OLIVEIRA, R. S. de; SOARES, L. H. B.; AYUD, M. A. Z.; SIQUEIRA, H. A. A.; FIGUEIRA, E. L. Z. Susceptibility of Anthonomus grandis (Cotton Boll Weevil) and Spodoptera frugiperda (Fall Armyworn) to a Cry1Ia-type toxin from a Brazilian Bacillus thuringiensis Strain. Journal of Biochemistry and Molecular Biology, v. 40, n. 5, p. 773-782, Sept. 2007.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
Biblioteca(s): Embrapa Cerrados. |
| |
131. | | RIBEIRO, T. P.; BASSO, M. F.; CARVALHO, M. H. de; MACEDO, L. L. P. de; SILVA, D. M. L. da; LOURENCO-TESSUTTI, I. T.; OLIVEIRA-NETO, O. B. de; CAMPOS-PINTO, E. R. de; LUCENA, W. A.; SILVA, M. C. M. da; TRIPODE, B. M. D.; ABREU-JARDIM, T. P. F.; MIRANDA, J. E.; ALVES-FERREIRA, M.; MORGANTE, C. V.; GROSSI-DE-SA, M. F. Stability and tissue-specific Cry10Aa overexpression improves cotton resistance to the cotton boll weevil. Biotechnology Research and Innovation, v. 3, p. 27-41, 2020.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
| |
Registros recuperados : 131 | |
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|