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Registro Completo |
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Biblioteca(s): |
Embrapa Meio-Norte; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
11/07/2019 |
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Data da última atualização: |
05/03/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, S. E. M.; GIMENES, M. A.; OLIVEIRA JUNIOR, J. O. L. de; COSTA, M. F.; GOMES, M. F. C.; VALENTE, S. E. S. |
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Afiliação: |
MARCOS APARECIDO GIMENES, Cenargen; JOSE OSCAR LUSTOSA DE O JUNIOR, CPAMN; M. F. COSTA, UFPI; M. F. C.GOMES, UESPI; S. E. S.VALENTE, UFPI. |
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Título: |
Consequences of accelerated aging for DNA integrity and seed germination of cowpea. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 18, n. 3, article gmr18304, 2019. |
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DOI: |
10.4238/gmr18304 |
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Idioma: |
Inglês |
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Conteúdo: |
Deterioration and loss of seed germination in the germplasm are related to the aging that occurs during the conservation process. The mechanisms related to the aging and death of seeds are not yet fully understood, but it is known that longevity can be affected by moisture content and temperature. We examined the effects of accelerated aging (A.A.) on the DNA of seeds of three different cultivars of cowpea (Vigna unguiculata) and compared the data to data from vigor and germination tests. The DNA of A.A. - seeds of BR 17 Gurgueia, BRS-Marataoã and BRS-Guariba cowpea cultivars were extracted and subjected to agarose gel electrophoresis. The increase in temperature, relative humidity and time used in A.A. culminated in a progressive and increasing degradation of seed DNA, which was expressed by continuous bands of DNA molecules with different lengths in the electrophoresis gel under all but the most extreme conditions (combinations 45°C/72 h and 45°C/96 h at 100% relative humidity) under which total disintegration of the DNA band was observed. This, in turn, was reflected in a progressive decrease in seed vigor. The combination of 35ºC/48 h was the most efficient for evaluating vigor in cowpea. The BR 17 Gurgueia and BRS-Guariba cultivars showed the highest values of vigor. A lack of DNA degradation in the laddering pattern on an electrophoresis gel is an indicator that DNA degradation and cell death in cowpea seeds did not occur by apoptosis but by non-programmed processes, such as Maillard reactions, lipid peroxidation and hydrolysis of sugars. MenosDeterioration and loss of seed germination in the germplasm are related to the aging that occurs during the conservation process. The mechanisms related to the aging and death of seeds are not yet fully understood, but it is known that longevity can be affected by moisture content and temperature. We examined the effects of accelerated aging (A.A.) on the DNA of seeds of three different cultivars of cowpea (Vigna unguiculata) and compared the data to data from vigor and germination tests. The DNA of A.A. - seeds of BR 17 Gurgueia, BRS-Marataoã and BRS-Guariba cowpea cultivars were extracted and subjected to agarose gel electrophoresis. The increase in temperature, relative humidity and time used in A.A. culminated in a progressive and increasing degradation of seed DNA, which was expressed by continuous bands of DNA molecules with different lengths in the electrophoresis gel under all but the most extreme conditions (combinations 45°C/72 h and 45°C/96 h at 100% relative humidity) under which total disintegration of the DNA band was observed. This, in turn, was reflected in a progressive decrease in seed vigor. The combination of 35ºC/48 h was the most efficient for evaluating vigor in cowpea. The BR 17 Gurgueia and BRS-Guariba cultivars showed the highest values of vigor. A lack of DNA degradation in the laddering pattern on an electrophoresis gel is an indicator that DNA degradation and cell death in cowpea seeds did not occur by apoptosis but by non-programmed processe... Mostrar Tudo |
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Palavras-Chave: |
Orthodox seeds; Seed bank. |
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Thesagro: |
Vigna Unguiculata. |
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Thesaurus Nal: |
Genetic resources. |
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Categoria do assunto: |
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G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/199456/1/gmr18304-consequences-accelerated-aging-dna-integrity-and-seed-germination-cowpea.pdf
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Marc: |
LEADER 02315naa a2200241 a 4500
001 2110566
005 2020-03-05
008 2019 bl uuuu u00u1 u #d
024 7 $a10.4238/gmr18304$2DOI
100 1 $aSANTOS, S. E. M.
245 $aConsequences of accelerated aging for DNA integrity and seed germination of cowpea.$h[electronic resource]
260 $c2019
520 $aDeterioration and loss of seed germination in the germplasm are related to the aging that occurs during the conservation process. The mechanisms related to the aging and death of seeds are not yet fully understood, but it is known that longevity can be affected by moisture content and temperature. We examined the effects of accelerated aging (A.A.) on the DNA of seeds of three different cultivars of cowpea (Vigna unguiculata) and compared the data to data from vigor and germination tests. The DNA of A.A. - seeds of BR 17 Gurgueia, BRS-Marataoã and BRS-Guariba cowpea cultivars were extracted and subjected to agarose gel electrophoresis. The increase in temperature, relative humidity and time used in A.A. culminated in a progressive and increasing degradation of seed DNA, which was expressed by continuous bands of DNA molecules with different lengths in the electrophoresis gel under all but the most extreme conditions (combinations 45°C/72 h and 45°C/96 h at 100% relative humidity) under which total disintegration of the DNA band was observed. This, in turn, was reflected in a progressive decrease in seed vigor. The combination of 35ºC/48 h was the most efficient for evaluating vigor in cowpea. The BR 17 Gurgueia and BRS-Guariba cultivars showed the highest values of vigor. A lack of DNA degradation in the laddering pattern on an electrophoresis gel is an indicator that DNA degradation and cell death in cowpea seeds did not occur by apoptosis but by non-programmed processes, such as Maillard reactions, lipid peroxidation and hydrolysis of sugars.
650 $aGenetic resources
650 $aVigna Unguiculata
653 $aOrthodox seeds
653 $aSeed bank
700 1 $aGIMENES, M. A.
700 1 $aOLIVEIRA JUNIOR, J. O. L. de
700 1 $aCOSTA, M. F.
700 1 $aGOMES, M. F. C.
700 1 $aVALENTE, S. E. S.
773 $tGenetics and Molecular Research$gv. 18, n. 3, article gmr18304, 2019.
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Registro original: |
Embrapa Meio-Norte (CPAMN) |
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| Registros recuperados : 11 | |
| 1. |  | SANTOS, P. A.; PINHEIRO, H. S. K.; CARVALHO JUNIOR, W. de; PEREIRA, N. R.; BHERING, S. B.; SILVA, I. L. Modeling soils physical-hydric attributes through algorithms for quantitative pedology in Guapi-Macacu watershed, RJ. In: PEDOMETRICS BRAZIL, 2., 2021, Rio de Janeiro. Annals [...]. Rio de Janeiro: Embrapa Solos, 2022. Não paginado. Evento online.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
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| 2. | | OLIVEIRA, P. F.; FIGUEIREDO, E. A. T.; MARQUES, P. A. H. F.; BORGES, M. de F.; BRUNO, L. M.; CARVALHO, C. B. M.; SILVA, I. L. Aeromonas spp. em cortes de frango resfriados e congelados produzidos industrialmente. In: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS, 8., 2009, Campinas. Resumos... Campinas: Universidade Estadual de Campinas, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 3. | | SANTOS, P. A. dos; PINHEIRO, H. S. K.; CARVALHO JUNIOR, W. de; SILVA, I. L. da; PEREIRA, N. R.; BHERING, S. B.; CEDDIA, M. B. Hydropedological digital mapping: machine learning applied to spectral VIS-IR and radiometric data dimensionality reduction. Revista Brasileira de Ciência do Solo, v. 47, e0220149, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 3 |
| Biblioteca(s): Embrapa Solos. |
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| 4. | | MOURA, M. F.; TAKEMURA, C. M.; SILVA, I. L. C.; TÁPIAS, L. M.; OLIVEIRA, C. T. de; BASSOI, L. H.; OLIVEIRA, S. R. de M. Metodologia para a construção de portfólios tecnológicos agrícolas a partir de publicações técnico-científicas. In: CONGRESSO BRASILEIRO DE AGROINFORMÁTICA, 11., 2017, Campinas. Ciência de dados na era da agricultura digital: anais. Campinas: Editora da Unicamp: Embrapa Informática Agropecuária, 2017. p. 537-546. SBIAgro 2017.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Instrumentação; Embrapa Territorial. |
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| 5. | | TERAO, D.; TAVARES, S. C. C. de H.; SILVA, I. L. do S. S. da; SARAIVA, A. C. M.; CHOUDHURY, M. M. Patologia pós-colheita de uva. In: LIMA, M. A. C. de. (Ed.). Uva de mesa: pós-colheita. 2. ed. rev. e ampl. Brasília, DF: Embrapa Informação Tecnológica; Petrolina: Embrapa Semi-Árido, 2007. cap. 4, p. 49-62. (Série Frutas do Brasil, 12).| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Semiárido. |
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| 8. | | SILVA, S. T. da; MELLO, J. M. de; ARCEBI JUNIOR, F. W.; REIS, A. A. dos; RAIMUNDO, M. R.; SILVA, I. L. G.; SCOLFORO, J. R. S. Uso de imagens de sensoriamento remoto para estratificação do cerrado em inventários florestais. Pesquisa Florestal Brasileira, Colombo, v. 34, n. 80, p. 337-343, out./dez. 2014.| Biblioteca(s): Embrapa Florestas. |
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| 9. | | NAKASONE, A. K.; SOBRINHO, R. S. A.; COUTINHO, I. B. L.; CAMPOS, K. R. A.; CARVALHO, J. E. U. de; NASCIMENTO, W. M. O. do; SILVA, I. L. do S. S. da; NECHET, K. de L. First report of Lasiodiplodia theobromae causing canker in Brazil nut. Australasian Plant Disease Notes, v. 17, n. 1, Article 27, Dec. 2022.| Tipo: Nota Técnica/Nota Científica |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Meio Ambiente. |
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| 10. | | CAMARGO, F. R. T. DE; SILVA, I. L.; BARROS, P. J. R.; ASCHERI, D. P. R.; RODOVALHO, R. S.; BELLIZZI, N. C.; ASCHERI, J. L. R.; TEIXEIRA, I. R.; DEVILLA, I. A.; CAMPOS, A. J. DE. Physiological quality of soybean seeds treated with carboxymethyl cellulose and fungicide. American Journal of Plant Sciences, v. 8, p. 2748-2757, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 11. | | SANTIAGO, E. J. A. de; PINTO, J. E. B. P.; CARVALHO, J. G. de; PAIVA, R.; CONCEIÇÃO, H. E. O. de; SANTOS, C. G. dos; SANTOS, B. R.; DECCETTI, S. F. C.; SILVA, I. L. do S. S.; GOMES, G. A. C. Crescimento de pimenta longa (Piper hispidinervium c.dc.) in vitro submetida a diferentes doses de nitrogênio e fósforo. In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 8., 2001, Ilhéus, BA. Resumos. Ilhéus: SBFV, 2001. p. 266. Cópia de trabalho editado em CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 11 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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