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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
30/01/2017 |
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Data da última atualização: |
30/01/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DELLAGNEZZE, B. M.; VASCONCELLOS, S. P. de; MELO, I. S. de; SANTOS NETO, E. V. dos; OLIVEIRA, V. M. de. |
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Afiliação: |
BRUNA MARTINS DELLAGNEZZE, CPQBA; SUSAN PANTAROTO DE VASCONCELLOS, UNIFESP; ITAMAR SOARES DE MELO, CNPMA; EUGENIO VAZ DOS SANTOS NETO, Petrobras; VALERIA MAIA DE OLIVEIRA, CPQBA. |
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Título: |
Evaluation of bacterial diversity recovered from petroleum samples using different physical matrices. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, São Paulo, v. 47, p. 314-321, 2016. |
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DOI: |
http://dx.doi.org/10.1016/j.bjm.2016.04.004 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Unraveling the microbial diversity and its complexity in petroleum reservoir environments has been a challenge throughout the years. Despite the techniques developed in order to improve crude oil, microbial enrichments using different culture conditions can be applied as a way to increase the recovery of DNA from environmentswith low cellular density for further microbiological analyses. This work aimed at the evaluation of different matrices(arenite, shale and polyurethane foam) as support materials for microbial growth and biofilm formation in enrichments using a biodegraded petroleum sample as inoculum in sulfate reducing condition. Subsequent microbial diversity characterization was carried out using Scanning Electronic Microscopy (SEM), Denaturing Gradient Gel Electrophoresis (DGGE) and 16S rRNA gene libraries in order to compare the microbial biomass yield, DNA recovery efficiency and diversity among the enrichments. The DNA from microbial communities in petroleum enrichments was purified according to a protocol established in this work and used for 16S rRNA amplification with bacterial generic primers. The PCR products were cloned, and positive clones were screened by Amplified Ribosomal DNA Restriction Analysis (ARDRA). Sequencing and phylogenetic analyses revealed that the bacterial community was mostly represented by members of the genera Petrotoga, Bacillus, Pseudomonas, Geobacillus and Rahnella. The use of different support materials in the enrichments yielded an increase in microbial biomass and biofilm formation, indicating that these materials may be employed for efficient biomass recovery from petroleum reservoir samples. Nonetheless, the most diverse microbiota were recovered from the biodegraded petroleum sample using polyurethane foam cubes as support material. MenosAbstract: Unraveling the microbial diversity and its complexity in petroleum reservoir environments has been a challenge throughout the years. Despite the techniques developed in order to improve crude oil, microbial enrichments using different culture conditions can be applied as a way to increase the recovery of DNA from environmentswith low cellular density for further microbiological analyses. This work aimed at the evaluation of different matrices(arenite, shale and polyurethane foam) as support materials for microbial growth and biofilm formation in enrichments using a biodegraded petroleum sample as inoculum in sulfate reducing condition. Subsequent microbial diversity characterization was carried out using Scanning Electronic Microscopy (SEM), Denaturing Gradient Gel Electrophoresis (DGGE) and 16S rRNA gene libraries in order to compare the microbial biomass yield, DNA recovery efficiency and diversity among the enrichments. The DNA from microbial communities in petroleum enrichments was purified according to a protocol established in this work and used for 16S rRNA amplification with bacterial generic primers. The PCR products were cloned, and positive clones were screened by Amplified Ribosomal DNA Restriction Analysis (ARDRA). Sequencing and phylogenetic analyses revealed that the bacterial community was mostly represented by members of the genera Petrotoga, Bacillus, Pseudomonas, Geobacillus and Rahnella. The use of different support materials in the enrichments ... Mostrar Tudo |
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Palavras-Chave: |
16S rRNA; Bacterial diversity; Microbial enrichment; Petroleum reservoir; Physical support. |
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Thesagro: |
Bactéria; Biodegradação; Biodiversidade; Petróleo. |
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Thesaurus Nal: |
Biodiversity. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/154264/1/2016AP35.pdf
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Marc: |
LEADER 02764naa a2200301 a 4500
001 2062180
005 2017-01-30
008 2016 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1016/j.bjm.2016.04.004$2DOI
100 1 $aDELLAGNEZZE, B. M.
245 $aEvaluation of bacterial diversity recovered from petroleum samples using different physical matrices.$h[electronic resource]
260 $c2016
520 $aAbstract: Unraveling the microbial diversity and its complexity in petroleum reservoir environments has been a challenge throughout the years. Despite the techniques developed in order to improve crude oil, microbial enrichments using different culture conditions can be applied as a way to increase the recovery of DNA from environmentswith low cellular density for further microbiological analyses. This work aimed at the evaluation of different matrices(arenite, shale and polyurethane foam) as support materials for microbial growth and biofilm formation in enrichments using a biodegraded petroleum sample as inoculum in sulfate reducing condition. Subsequent microbial diversity characterization was carried out using Scanning Electronic Microscopy (SEM), Denaturing Gradient Gel Electrophoresis (DGGE) and 16S rRNA gene libraries in order to compare the microbial biomass yield, DNA recovery efficiency and diversity among the enrichments. The DNA from microbial communities in petroleum enrichments was purified according to a protocol established in this work and used for 16S rRNA amplification with bacterial generic primers. The PCR products were cloned, and positive clones were screened by Amplified Ribosomal DNA Restriction Analysis (ARDRA). Sequencing and phylogenetic analyses revealed that the bacterial community was mostly represented by members of the genera Petrotoga, Bacillus, Pseudomonas, Geobacillus and Rahnella. The use of different support materials in the enrichments yielded an increase in microbial biomass and biofilm formation, indicating that these materials may be employed for efficient biomass recovery from petroleum reservoir samples. Nonetheless, the most diverse microbiota were recovered from the biodegraded petroleum sample using polyurethane foam cubes as support material.
650 $aBiodiversity
650 $aBactéria
650 $aBiodegradação
650 $aBiodiversidade
650 $aPetróleo
653 $a16S rRNA
653 $aBacterial diversity
653 $aMicrobial enrichment
653 $aPetroleum reservoir
653 $aPhysical support
700 1 $aVASCONCELLOS, S. P. de
700 1 $aMELO, I. S. de
700 1 $aSANTOS NETO, E. V. dos
700 1 $aOLIVEIRA, V. M. de
773 $tBrazilian Journal of Microbiology, São Paulo$gv. 47, p. 314-321, 2016.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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| 43. |  | OLIVEIRA, V. M. de; CARNEIRO, A. V.; ROCHA, B. B.; NEVES, A. L. A. Saúde animal: riscos e consequências. In: CARVALHO, G. R.; CARNEIRO, A. V.; YAMAGUCHI, L. C. T.; MARTINS, P. do C.; HOTT, M. C.; REIS FILHO, R. J. C.; OLIVEIRA, M. A. de (Ed.). Competitividade da cadeia produtiva do leite em Pernambuco. Juiz de Fora: Embrapa Gado de Leite, 2009. p. 221-237| Tipo: Capítulo em Livro Técnico-Científico |
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| 46. | | OLIVEIRA, V. M. de; TORRES, R. de A.; CARVALHO, D. M. F.; ROCHA, B. B.; CARNEIRO, F. M. Atividades relacionadas à ordenha que podem alterar a saúde do úbere, a qualidade e a composição do leite. In: TORRES, R. de A.; OLIVEIRA, V. M.; RODRIGUES, C. A. F. (Ed.). Políticas e tecnologias para o desenvolvimento da pecuária de leite familiar da Zona da Mata Mineira. Juiz de Fora: Embrapa Gado de Leite, 2009. p. 113-128.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 47. | | VALARINI, M. J.; BROCHINI, D.; FANTINATTI, F.; OLIVEIRA, V. M.; MANFIO, G. P.; MELO, I. S. de. Bactérias diazotróficas endofíticas isoladas de espécies de Brachiaria. In: SIMPÓSIO, 4., REUNIÃO DE AVALIAÇÃO DO PROGRAMA BIOTA/FAPESP, 4., Águas de Lindóia, 2003. Anais... Águas de Lindóia: FAPESP, 2003.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 48. | | AVILA, L. A.; ANDREOTE, F. D.; SANTOS, S. N.; KAVAMURA, V.; OLIVEIRA, V. M.; MELO, I. S. de. Adaptation versus competition in the composition of the community of Pseudomonas spp. in mangroves. In: INTERNATIONAL CONGRESS ON EXTREMOPHILES, 8., 2010, Ponta Delgada. Book of Abstracts... Ponta Delgada, 2010. 1 CD-ROM. p. 144. p. 245| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 56. | | PEREIRA, G. G.; GOLDFEDER, G. T.; YAMAKI, F. L.; OLIVEIRA, V. M. C.; LARSSON, M. H. M. A. Myocardial velocities obtained by pulsed tissue Doppler in English Cocker Spaniels with dilated cardiomyopathy and congestive heart failure. Pesquisa Veterinária Brasileira, Brasília, DF, v. 39, n. 9, p. 851-856, set. 2016.| Biblioteca(s): Embrapa Unidades Centrais. |
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