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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
28/07/2010 |
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Data da última atualização: |
18/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOARES, V. L. F.; RODRIGUES, S. M.; OLIVEIRA, T. M. de; QUEIROZ, T. O. de; LIMA, L. S.; HORA-JÚNIOR, B. T.; GRAMACHO, K. P.; MICHELI, F.; CASCARDO, J. C. M.; OTONI, W. C.; GESTEIRA, A. S.; COSTA, M. G. C. |
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Afiliação: |
VIRGÍNIA L. F. SOARES, UESC; SIMONE M. RODRIGUES, UFV; TAHISE M. DE OLIVEIRA, UESC; TALISSON O. DE QUEIROZ, UESC; LÍVIA S. LIMA, CEPLAC; BRAZ T. HORA-JÚNIOR, UESC; KARINA P. GRAMACHO, CEPLAC; FABIENNE MICHELI, UESC; JÚLIO C. M. CASCARDO, UESC; WAGNER C. OTONI, UFV; ABELMON DA SILVA GESTEIRA, CNPMF; MARCIO G. C. COSTA, UESC. |
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Título: |
Unraveling new genes associated with seed development and metabolism in Bixa orellana L. by expressed sequence tag (EST) analysis. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Molecular Biology Reports, New York, jun. 2010. |
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ISSN: |
1572-9818 |
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DOI: |
10.1007/s11033-010-0234-8 |
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Idioma: |
Inglês |
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Notas: |
On line first, with supplementary materials |
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Conteúdo: |
The tropical tree Bixa orellana L. produces a range of secondary metabolites which biochemical and molecular biosynthesis basis are not well understood. In this work we have characterized a set of ESTs from a nonnormalized cDNA library of B. orellana seeds to obtain information about the main developmental and metabolic processes taking place in developing seeds and their associated genes. After sequencing a set of randomly selected clones, most of the sequences were assigned with putative functions based on similarity, GO annotations and protein domains. The most abundant transcripts encoded proteins associated with cell wall (prolyl 4-hydroxylase), fatty acid (acyl carrier protein), and hormone/flavonoid (2OG-Fe oxygenase) synthesis, germination (MADS FLClike protein) and embryo development (AP2/ERF transcription factor) regulation, photosynthesis (chlorophyll a?b binding protein), cell elongation (MAP65-1a), and stress responses (metallothionein- and thaumatin-like proteins). Enzymes were assigned to 16 different metabolic pathways related to both primary and secondary metabolisms. Characterization of two candidate genes of the bixin biosynthetic pathway, BoCCD and BoOMT, showed that they belong, respectively, to the carotenoid-cleavage dioxygenase 4 (CCD4) and caffeic acid O-methyltransferase (COMT) families, and are up-regulated during seed development. It indicates their involvement in the synthesis of this commercially important carotenoid pigment in seeds of B. orellana. Most of the genes identified here are the first representatives of their gene families in B. orellana. MenosThe tropical tree Bixa orellana L. produces a range of secondary metabolites which biochemical and molecular biosynthesis basis are not well understood. In this work we have characterized a set of ESTs from a nonnormalized cDNA library of B. orellana seeds to obtain information about the main developmental and metabolic processes taking place in developing seeds and their associated genes. After sequencing a set of randomly selected clones, most of the sequences were assigned with putative functions based on similarity, GO annotations and protein domains. The most abundant transcripts encoded proteins associated with cell wall (prolyl 4-hydroxylase), fatty acid (acyl carrier protein), and hormone/flavonoid (2OG-Fe oxygenase) synthesis, germination (MADS FLClike protein) and embryo development (AP2/ERF transcription factor) regulation, photosynthesis (chlorophyll a?b binding protein), cell elongation (MAP65-1a), and stress responses (metallothionein- and thaumatin-like proteins). Enzymes were assigned to 16 different metabolic pathways related to both primary and secondary metabolisms. Characterization of two candidate genes of the bixin biosynthetic pathway, BoCCD and BoOMT, showed that they belong, respectively, to the carotenoid-cleavage dioxygenase 4 (CCD4) and caffeic acid O-methyltransferase (COMT) families, and are up-regulated during seed development. It indicates their involvement in the synthesis of this commercially important carotenoid pigment in seeds of B. orell... Mostrar Tudo |
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Palavras-Chave: |
Apocarotenoids; Bixa orellana L; Bixin; Carotenoid-cleavage dioxygenase; O-methyltransferase; Sequencia genetica. |
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Thesagro: |
Caroteno; Pigmento; Urucum. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/858658/1/Unraveling-new-genes-associated-with-seed-development-and-metabolism-in-Bixa-orellana-L.-by-expressed-sequence-tag-nalysis..pdf
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Marc: |
LEADER 02774naa a2200397 a 4500
001 1858658
005 2022-08-18
008 2010 bl uuuu u00u1 u #d
022 $a1572-9818
024 7 $a10.1007/s11033-010-0234-8$2DOI
100 1 $aSOARES, V. L. F.
245 $aUnraveling new genes associated with seed development and metabolism in Bixa orellana L. by expressed sequence tag (EST) analysis.
260 $c2010
500 $aOn line first, with supplementary materials
520 $aThe tropical tree Bixa orellana L. produces a range of secondary metabolites which biochemical and molecular biosynthesis basis are not well understood. In this work we have characterized a set of ESTs from a nonnormalized cDNA library of B. orellana seeds to obtain information about the main developmental and metabolic processes taking place in developing seeds and their associated genes. After sequencing a set of randomly selected clones, most of the sequences were assigned with putative functions based on similarity, GO annotations and protein domains. The most abundant transcripts encoded proteins associated with cell wall (prolyl 4-hydroxylase), fatty acid (acyl carrier protein), and hormone/flavonoid (2OG-Fe oxygenase) synthesis, germination (MADS FLClike protein) and embryo development (AP2/ERF transcription factor) regulation, photosynthesis (chlorophyll a?b binding protein), cell elongation (MAP65-1a), and stress responses (metallothionein- and thaumatin-like proteins). Enzymes were assigned to 16 different metabolic pathways related to both primary and secondary metabolisms. Characterization of two candidate genes of the bixin biosynthetic pathway, BoCCD and BoOMT, showed that they belong, respectively, to the carotenoid-cleavage dioxygenase 4 (CCD4) and caffeic acid O-methyltransferase (COMT) families, and are up-regulated during seed development. It indicates their involvement in the synthesis of this commercially important carotenoid pigment in seeds of B. orellana. Most of the genes identified here are the first representatives of their gene families in B. orellana.
650 $aCaroteno
650 $aPigmento
650 $aUrucum
653 $aApocarotenoids
653 $aBixa orellana L
653 $aBixin
653 $aCarotenoid-cleavage dioxygenase
653 $aO-methyltransferase
653 $aSequencia genetica
700 1 $aRODRIGUES, S. M.
700 1 $aOLIVEIRA, T. M. de
700 1 $aQUEIROZ, T. O. de
700 1 $aLIMA, L. S.
700 1 $aHORA-JÚNIOR, B. T.
700 1 $aGRAMACHO, K. P.
700 1 $aMICHELI, F.
700 1 $aCASCARDO, J. C. M.
700 1 $aOTONI, W. C.
700 1 $aGESTEIRA, A. S.
700 1 $aCOSTA, M. G. C.
773 $tMolecular Biology Reports, New York, jun. 2010.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Rondônia. Para informações adicionais entre em contato com cpafro.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
10/01/2008 |
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Data da última atualização: |
01/09/2009 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Circulação/Nível: |
-- - -- |
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Autoria: |
RODRIGUES, V. G. S.; SANTOS, M. R. A. dos; LIMA, M. R. de. |
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Afiliação: |
Vanda Gorete Souza Rodrigues, Embrapa Rondônia; Maurício Reginaldo Alves dos Santos, Embrapa Rondônia. |
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Título: |
Agricultura urbana na região de Porto Velho - RO: concepção e contexto agroecológico. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
In: REUNIÃO AMAZÔNICA DE AGROECOLOGIA, 1., 2007, Manaus. A agroecologia no contexto amazônico: palestras, relatos de experiência e resumos. Manaus: Embrapa Amazônia Ocidental, 2007. 1 CD ROM. |
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Idioma: |
Português |
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Palavras-Chave: |
Agricultura urbana; Porto Velho. |
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Thesagro: |
Agricultura Familiar; Desenvolvimento Sustentável. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00711naa a2200181 a 4500
001 1698814
005 2009-09-01
008 2007 bl --- 0-- u #d
100 1 $aRODRIGUES, V. G. S.
245 $aAgricultura urbana na região de Porto Velho - RO$bconcepção e contexto agroecológico.
260 $c2007
650 $aAgricultura Familiar
650 $aDesenvolvimento Sustentável
653 $aAgricultura urbana
653 $aPorto Velho
700 1 $aSANTOS, M. R. A. dos
700 1 $aLIMA, M. R. de
773 $tIn: REUNIÃO AMAZÔNICA DE AGROECOLOGIA, 1., 2007, Manaus. A agroecologia no contexto amazônico: palestras, relatos de experiência e resumos. Manaus: Embrapa Amazônia Ocidental, 2007. 1 CD ROM.
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Embrapa Rondônia (CPAF-RO) |
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