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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças; Embrapa Unidades Centrais. |
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Data corrente: |
05/10/2016 |
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Data da última atualização: |
24/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARRER FILHO, R.; DIAS, V. D.; OLIVEIRA, R. M. de; DIANESE, E. de C.; BOITEUX, L. S.; CUNHA, M. G. da. |
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Afiliação: |
RENATO CARRER FILHO, UFGO; VANESSA DUARTE DIAS, UFGO; RENATA MARIA DE OLIVEIRA, UFGO; ÉRICO DE CAMPOS DIANESE, UFGO; LEONARDO SILVA BOITEUX, CNPH; MARCOS GOMES DA CUNHA, UFGO. |
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Título: |
Detecção simultânea de fatores de resistência à murcha de fusário do tomateiro por meio de PCR multiplex. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 51, n. 8, p. 925-932, ago. 2016. |
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Idioma: |
Português |
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Notas: |
Título em inglês: Simultaneous detection of tomato resistance factors to Fusarium wilt in tomato by multiplex PCR. |
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Conteúdo: |
O objetivo deste trabalho foi elaborar e validar um protocolo de detecção simultânea, via reação em cadeia da polimerase multiplex (PCR multiplex), de regiões genômicas do tomateiro (Solanum lycopersicum) associadas a fatores de resistência às três raças fisiológicas de Fusarium oxysporum f. sp. lycopersici (FOL). Os pares de iniciadores empregados foram SSR?67 (específico para o gene I?1), TFusrr (específico para o gene I?2) e SSRD (específico para o gene I?3). Os resultados de genotipagem com marcadores moleculares foram comparados aos resultados de fenotipagem de uma coleção de germoplasma de tomateiro, em bioensaios de inoculação de isolados das três raças de FOL em plântulas, pelo método de imersão das raízes. A resistência
ou a suscetibilidade foi confirmada por PCR, por meio de visualização dos âmplicons específicos para as regiões?alvo ligadas aos fatores de resistência às distintas raças de FOL. O protocolo elaborado para o uso conjunto dos marcadores moleculares, em PCR multiplex, permite a seleção de acessos de tomateiro resistentes às raças 1, 2, e 3 de F. oxysporum f. sp. lycopersici de maneira similar à realizada com a utilização de cada um separadamente. O PCR multiplex representa uma ferramenta viável para monitorar a incorporação desses fatores de resistência em linhagens de tomateiro. |
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Palavras-Chave: |
Assisted breeding; Seleção assistida. |
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Thesagro: |
Fusarium Oxysporum; Resistência genética. |
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Thesaurus Nal: |
Genetic resistance; Solanum lycopersicum. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/148394/1/Deteccao-simultanea.pdf
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Marc: |
LEADER 02301naa a2200265 a 4500
001 2054150
005 2017-05-24
008 2016 bl uuuu u00u1 u #d
100 1 $aCARRER FILHO, R.
245 $aDetecção simultânea de fatores de resistência à murcha de fusário do tomateiro por meio de PCR multiplex.
260 $c2016
500 $aTítulo em inglês: Simultaneous detection of tomato resistance factors to Fusarium wilt in tomato by multiplex PCR.
520 $aO objetivo deste trabalho foi elaborar e validar um protocolo de detecção simultânea, via reação em cadeia da polimerase multiplex (PCR multiplex), de regiões genômicas do tomateiro (Solanum lycopersicum) associadas a fatores de resistência às três raças fisiológicas de Fusarium oxysporum f. sp. lycopersici (FOL). Os pares de iniciadores empregados foram SSR?67 (específico para o gene I?1), TFusrr (específico para o gene I?2) e SSRD (específico para o gene I?3). Os resultados de genotipagem com marcadores moleculares foram comparados aos resultados de fenotipagem de uma coleção de germoplasma de tomateiro, em bioensaios de inoculação de isolados das três raças de FOL em plântulas, pelo método de imersão das raízes. A resistência ou a suscetibilidade foi confirmada por PCR, por meio de visualização dos âmplicons específicos para as regiões?alvo ligadas aos fatores de resistência às distintas raças de FOL. O protocolo elaborado para o uso conjunto dos marcadores moleculares, em PCR multiplex, permite a seleção de acessos de tomateiro resistentes às raças 1, 2, e 3 de F. oxysporum f. sp. lycopersici de maneira similar à realizada com a utilização de cada um separadamente. O PCR multiplex representa uma ferramenta viável para monitorar a incorporação desses fatores de resistência em linhagens de tomateiro.
650 $aGenetic resistance
650 $aSolanum lycopersicum
650 $aFusarium Oxysporum
650 $aResistência genética
653 $aAssisted breeding
653 $aSeleção assistida
700 1 $aDIAS, V. D.
700 1 $aOLIVEIRA, R. M. de
700 1 $aDIANESE, E. de C.
700 1 $aBOITEUX, L. S.
700 1 $aCUNHA, M. G. da
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 51, n. 8, p. 925-932, ago. 2016.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
26/05/2010 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BRANDÃO, F. Z.; ARASHIRO, E. K. N.; HENRY, M.; FIGUEIRA, L. M.; SOUZA, J. M. C.; VIANA, J. H. M.; FONSECA, J. F. da. |
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Afiliação: |
Universidade Federal Fluminense, Niteroi, RJ; Universidade Federal de Minas Gerais (UGMG), Belo Horizonte, MG; UFMG; Universidade Federal Fluminense; Universidade Federal de Viçosa (UFV), Viçosa, MG; JOAO HENRIQUE MOREIRA VIANA, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC. |
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Título: |
Evaluation of superovulatory response in Santa Inês ewes by ultrasonography and laparoscopy. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Reproduction, Fertility and Development, v. 22, n. 1, p. 242, 2010. |
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DOI: |
10.1071/RDv22n1Ab167 |
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Idioma: |
Inglês |
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Notas: |
Edição dos Proceedings of the Annual Conference of the International Embryo Transfer Society, Córdoba, Argentina, 9?12 January 2010. |
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Conteúdo: |
The development and use of non-invasive techniques would reduce risks of surgery sequels on the same animal and use of the donor could be optimized. The aim of this study was to evaluate the accuracy of ultrasonography performed prior to embryo collection to estimate superovulation response in sheep. Fifteen pluriparous Santa Inês sheep, 2 to 5 year old, with an average body weight of 46.79 ± 6.00 kg and body condition score of 2.96 ± 0.32 (1 to 5 scale) were allocated into 3 groups (GI, GII, GIII) in a cross-over design. In GI, intravaginal sponges (60 mg of medroxyprogesterone acetate; Progespon®, Schering Plough Animal Health, São Paulo, Brazil) were inserted (Day 0) and maintained for 14 days, and the superovulatory (SOV) protocol started on Day 12. In GII and GIII, intravaginal sponges were inserted (Day 0) and maintained for 6 days. On Day 5, the animals were treated with 300 IU of eCG (Novormon 5000®, Schering Plough Animal Health) and 5 mg of dinoprost (Lutalyse® Pfizer Animal Health, São Paulo, Brazil) i.m. Animals in GIII received 0.025 mg of gonadorelin acetate (Gestran-Plus®, Tecnopec, São Paulo, Brazil) i.m. 12 h after sponge withdrawal. In GII and GIII, the SOV protocol started 48 h after sponge removal and a new sponge was inserted immediately after its removal. The SOV protocol in all groups consisted of 200 mg of pFSH (Folltropin-V®, Tecnopec) administered in 6 decreasing doses given every 12 h (50/50, 30/30, and 20/20 mg). At the time of the fifth dose of pFSH, 5 mg of dinoprost was administered i.m. and the sponges were removed. Animals were bred by a sexually mature ram twice a day until the end of estrus. Prior to embryo collection, an ultrasonographic evaluation (5 MHz, Aloka SSD-500, Tokyo, Japan) was performed to determine the number of CL present on both ovaries. The number of CL was further evaluated by laparoscopy, when it was possible to detect poor responders or even non-ovulating animals. Statistical analysis was performed using all tests at the 95% confidence interval by SAEG program. Results are presented as mean ± SE. The number of CL was not different among all groups. The total number (GI, GII, and GIII) of CL determined by ultrasonography (9.09 ± 5.01) was not different (P > 0.05) from that observed by laparoscopy (8.87 ± 5.25). A significant correlation (r = 0.56, P < 0.0005) between evaluation performed by ultrasonography and laparoscopy was observed. These results suggest that ultrasonography can be used to determine the response to the superovulatory protocol. As embryo collection in sheep is performed mainly by surgical techniques, the implementation of non-invasive techniques such as ultrasonography could avoid unnecessary surgeries on animals that did not respond to the SOV protocol, therefore preventing early culling of embryo donors. Top Print this pagePrint Email this page View Issue Contents Abstract Export Citation Tools Print Bookmark Email this page Early Alert Subscribe to our Early Alerts for the latest journal issue contents. MenosThe development and use of non-invasive techniques would reduce risks of surgery sequels on the same animal and use of the donor could be optimized. The aim of this study was to evaluate the accuracy of ultrasonography performed prior to embryo collection to estimate superovulation response in sheep. Fifteen pluriparous Santa Inês sheep, 2 to 5 year old, with an average body weight of 46.79 ± 6.00 kg and body condition score of 2.96 ± 0.32 (1 to 5 scale) were allocated into 3 groups (GI, GII, GIII) in a cross-over design. In GI, intravaginal sponges (60 mg of medroxyprogesterone acetate; Progespon®, Schering Plough Animal Health, São Paulo, Brazil) were inserted (Day 0) and maintained for 14 days, and the superovulatory (SOV) protocol started on Day 12. In GII and GIII, intravaginal sponges were inserted (Day 0) and maintained for 6 days. On Day 5, the animals were treated with 300 IU of eCG (Novormon 5000®, Schering Plough Animal Health) and 5 mg of dinoprost (Lutalyse® Pfizer Animal Health, São Paulo, Brazil) i.m. Animals in GIII received 0.025 mg of gonadorelin acetate (Gestran-Plus®, Tecnopec, São Paulo, Brazil) i.m. 12 h after sponge withdrawal. In GII and GIII, the SOV protocol started 48 h after sponge removal and a new sponge was inserted immediately after its removal. The SOV protocol in all groups consisted of 200 mg of pFSH (Folltropin-V®, Tecnopec) administered in 6 decreasing doses given every 12 h (50/50, 30/30, and 20/20 mg). At the time of the fifth dose of p... Mostrar Tudo |
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Palavras-Chave: |
Raça Santa Inês; Resposta ovariana; Ultra-Som. |
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Thesagro: |
Ovino; Reprodução animal; Superovulação; Transferência de embrião. |
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Thesaurus NAL: |
Animal reproduction; Sheep; Superovulation. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/61354/1/rac-EVALUATION-OF-SUPEROVULATORY.pdf
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Marc: |
LEADER 04138nam a2200325 a 4500
001 1853920
005 2019-09-23
008 2010 bl uuuu u00u1 u #d
024 7 $a10.1071/RDv22n1Ab167$2DOI
100 1 $aBRANDÃO, F. Z.
245 $aEvaluation of superovulatory response in Santa Inês ewes by ultrasonography and laparoscopy.$h[electronic resource]
260 $aReproduction, Fertility and Development, v. 22, n. 1, p. 242$c2010
500 $aEdição dos Proceedings of the Annual Conference of the International Embryo Transfer Society, Córdoba, Argentina, 9?12 January 2010.
520 $aThe development and use of non-invasive techniques would reduce risks of surgery sequels on the same animal and use of the donor could be optimized. The aim of this study was to evaluate the accuracy of ultrasonography performed prior to embryo collection to estimate superovulation response in sheep. Fifteen pluriparous Santa Inês sheep, 2 to 5 year old, with an average body weight of 46.79 ± 6.00 kg and body condition score of 2.96 ± 0.32 (1 to 5 scale) were allocated into 3 groups (GI, GII, GIII) in a cross-over design. In GI, intravaginal sponges (60 mg of medroxyprogesterone acetate; Progespon®, Schering Plough Animal Health, São Paulo, Brazil) were inserted (Day 0) and maintained for 14 days, and the superovulatory (SOV) protocol started on Day 12. In GII and GIII, intravaginal sponges were inserted (Day 0) and maintained for 6 days. On Day 5, the animals were treated with 300 IU of eCG (Novormon 5000®, Schering Plough Animal Health) and 5 mg of dinoprost (Lutalyse® Pfizer Animal Health, São Paulo, Brazil) i.m. Animals in GIII received 0.025 mg of gonadorelin acetate (Gestran-Plus®, Tecnopec, São Paulo, Brazil) i.m. 12 h after sponge withdrawal. In GII and GIII, the SOV protocol started 48 h after sponge removal and a new sponge was inserted immediately after its removal. The SOV protocol in all groups consisted of 200 mg of pFSH (Folltropin-V®, Tecnopec) administered in 6 decreasing doses given every 12 h (50/50, 30/30, and 20/20 mg). At the time of the fifth dose of pFSH, 5 mg of dinoprost was administered i.m. and the sponges were removed. Animals were bred by a sexually mature ram twice a day until the end of estrus. Prior to embryo collection, an ultrasonographic evaluation (5 MHz, Aloka SSD-500, Tokyo, Japan) was performed to determine the number of CL present on both ovaries. The number of CL was further evaluated by laparoscopy, when it was possible to detect poor responders or even non-ovulating animals. Statistical analysis was performed using all tests at the 95% confidence interval by SAEG program. Results are presented as mean ± SE. The number of CL was not different among all groups. The total number (GI, GII, and GIII) of CL determined by ultrasonography (9.09 ± 5.01) was not different (P > 0.05) from that observed by laparoscopy (8.87 ± 5.25). A significant correlation (r = 0.56, P < 0.0005) between evaluation performed by ultrasonography and laparoscopy was observed. These results suggest that ultrasonography can be used to determine the response to the superovulatory protocol. As embryo collection in sheep is performed mainly by surgical techniques, the implementation of non-invasive techniques such as ultrasonography could avoid unnecessary surgeries on animals that did not respond to the SOV protocol, therefore preventing early culling of embryo donors. Top Print this pagePrint Email this page View Issue Contents Abstract Export Citation Tools Print Bookmark Email this page Early Alert Subscribe to our Early Alerts for the latest journal issue contents.
650 $aAnimal reproduction
650 $aSheep
650 $aSuperovulation
650 $aOvino
650 $aReprodução animal
650 $aSuperovulação
650 $aTransferência de embrião
653 $aRaça Santa Inês
653 $aResposta ovariana
653 $aUltra-Som
700 1 $aARASHIRO, E. K. N.
700 1 $aHENRY, M.
700 1 $aFIGUEIRA, L. M.
700 1 $aSOUZA, J. M. C.
700 1 $aVIANA, J. H. M.
700 1 $aFONSECA, J. F. da
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