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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
17/12/2009 |
Data da última atualização: |
19/03/2010 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
RAMOS, C. A. N.; ARAÚJO, F. R.; SOUZA, I. I. F.; OLIVEIRA, R. H. M. de; ELISEI, C.; SOARES, C. O.; SACCO, A. M. S.; ROSINHA, G. M. S.; ALVES, L. C. |
Afiliação: |
CARLOS ALBERTO DO NASCIMENTO RAMOS, UFRPE; FLABIO RIBEIRO ARAUJO, CNPGC; INGRID IEDA FERNANDO DE SOUZA, BOLSISTA - CNPGC; RENATO HENRIQUE MARCAL DE OLIVEIRA, CNPGC; CARINA ELISEI, BOLSISTA - CNPGC; CLEBER OLIVEIRA SOARES, CNPGC; ANA MARIA SASTRE SACCO, CPPSUL; GRACIA MARIA SOARES ROSINHA, CNPGC; LEUCIO C. ALVES, UFRPE. |
Título: |
Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, n.7, p. 998-1002, nov. 2009. |
Idioma: |
Inglês |
Conteúdo: |
Babesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovis as well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis. The aims of the present paper were to analyse the genetic conservation of the P0 gene among Brazilian isolates of B. bovis and produce recombinant P0 (rPO) from a Brazilian isolate and evaluate its antigenicity with sera from cattle naturally or experimentally infected with B. bovis and sera from cattle experimentally infected with B. bigemina in an enzyme-linked immunosorbent assay (ELISA). MenosBabesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovis as well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis. The aims of the present paper were to analyse the genetic conservation of the P0 gene among Brazilian isolates of B. bovis and produce recombinant P0 (rPO) from a Brazilian isolate and evaluate its antigenicity with sera from cattle naturally or experimentally infected with B. bovis and sera from cattle experimentally infected with B. bige... Mostrar Tudo |
Palavras-Chave: |
Babesiose bovina. |
Thesagro: |
Babesia bovis; Bovino; Sanidade animal. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 02329naa a2200265 a 4500 001 1578251 005 2010-03-19 008 2009 bl --- 0-- u #d 100 1 $aRAMOS, C. A. N. 245 $aMolecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis. 260 $c2009 520 $aBabesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovis as well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis. The aims of the present paper were to analyse the genetic conservation of the P0 gene among Brazilian isolates of B. bovis and produce recombinant P0 (rPO) from a Brazilian isolate and evaluate its antigenicity with sera from cattle naturally or experimentally infected with B. bovis and sera from cattle experimentally infected with B. bigemina in an enzyme-linked immunosorbent assay (ELISA). 650 $aBabesia bovis 650 $aBovino 650 $aSanidade animal 653 $aBabesiose bovina 700 1 $aARAÚJO, F. R. 700 1 $aSOUZA, I. I. F. 700 1 $aOLIVEIRA, R. H. M. de 700 1 $aELISEI, C. 700 1 $aSOARES, C. O. 700 1 $aSACCO, A. M. S. 700 1 $aROSINHA, G. M. S. 700 1 $aALVES, L. C. 773 $tMemórias do Instituto Oswaldo Cruz, Rio de Janeiro$gv. 104, n.7, p. 998-1002, nov. 2009.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registro Completo
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
15/12/2011 |
Data da última atualização: |
16/08/2013 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
PELEGRINI, L. L.; LEANDRO F. de OLIVEIRA; FERREIRA, D. L.; PADILHA, J. H. D.; SILVA, R. C. da; MATTIA, R. C.; DEGENHARDT-GOLDBACH, J.; QUOIRIN, M. |
Afiliação: |
LUCIANA L. PELEGRINI, Aluna Pós-Graduação em Produção; LEANDRO F. de OLIVEIRA, Aluno de pós-graduação UFPR; DANIELLE L. FERREIRA, Aluna de pós-graduação UFPR; JOÃO H. D. PADILHA, Aluno de pós-graduação UFPR; RODRIGO C. da SILVA, Aluno de Graduação UFPR; RAFAELA C. MATTIA, Aluna pós-graduação UFPR; JULIANA DEGENHARDT GOLDBACH, CNPF; MARGUERITE QUOIRIN, UFPR. |
Título: |
Indução de calogênese em explantes foliares de Calophyllum brasiliense. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 18.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 5., 2011, Joinville. Resumos... Joinville: Sociedade Brasileira de Plantas Ornamentais e Associação Brasileira de Cultura de Tecidos de Plantas, 2011. |
Idioma: |
Português |
Thesagro: |
Propagação Vegetativa. |
Thesaurus NAL: |
Calophyllum brasiliense. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/50416/1/CBFPO-Inducao.pdf
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Marc: |
LEADER 00853nam a2200205 a 4500 001 1909909 005 2013-08-16 008 2011 bl uuuu u00u1 u #d 100 1 $aPELEGRINI, L. L. 245 $aIndução de calogênese em explantes foliares de Calophyllum brasiliense. 260 $aIn: CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 18.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 5., 2011, Joinville. Resumos... Joinville: Sociedade Brasileira de Plantas Ornamentais e Associação Brasileira de Cultura de Tecidos de Plantas$c2011 650 $aCalophyllum brasiliense 650 $aPropagação Vegetativa 700 1 $aLEANDRO F. de OLIVEIRA 700 1 $aFERREIRA, D. L. 700 1 $aPADILHA, J. H. D. 700 1 $aSILVA, R. C. da 700 1 $aMATTIA, R. C. 700 1 $aDEGENHARDT-GOLDBACH, J. 700 1 $aQUOIRIN, M.
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