Data/hora: 12/06/2024 19:05:58

Biblioteca(s): | Embrapa Gado de Corte.
Data corrente: | 08/08/2011
Data da última atualização: | 08/08/2011
Autoria: | RAMOS, C. A. N.; ARAUJO, F. R.; SOUZA, I. I. F.; BACANELLI, G.; LUIZ, H. L.; RUSSI, L. S.; OLIVEIRA, R. H. M. de; SOARES, C. O.; ROSINHA, G. M. S.; ALVES, L. C.
Afiliação: | Carlos Alberto do Nascimento Ramos, Bolsista; FLABIO RIBEIRO ARAUJO, CNPGC; Ingrid I.F. Souza, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco; G. Bacanelli, BOLSISTA; Hera L. Luiz, Bolsista; Lívia S. Russi, Bolsista; RENATO HENRIQUE MARCAL DE OLIVEIRA, CNPGC; CLEBER OLIVEIRA SOARES, CNPGC; GRACIA MARIA SOARES ROSINHA, CNPGC; Leucio C. Alves, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco.
Título: | Real-time polymerase chain reaction based on msa2c gene for detection of Babesia bovis.
Ano de publicação: | 2011
Fonte/Imprenta: | Veterinary Parasitology, v.176, p.79-83, Feb. 2011. Issue 1.
Idioma: | Inglês
Conteúdo: | This paper reports a quantitative real-time polymerase chain reaction (q-PCR) based on the msa2c gene and standardized with Platinum SYBR Green/ROX for the detection of Babesia bovis in cattle. The msa2c q-PCR amplified a DNA fragment with average dissociation temperature of 77.41 &#9702;C (±0.25 &#9702;C). No amplification was detected when DNA from B. bigemina, A. marginale or Bos taurus was used as the template. The detection limit of the msa2c q-PCR was 1000 copies per ml of blood sample, with a linear correlation between the number of msa2c copies and threshold cycle. The comparison between msa2c q-PCR and conventional PCR for cytochrome b revealed 88.8% agreement, with a Kappa index of 0.75. In the comparison between msa2c q-PCR and an enzyme-linked immunosorbent assay (ELISA) with semi-purified B. bovis antigen, agreement was 96.3% and the Kappa index was 0.91. The agreement between three tests was 85.8%. The msa2c q-PCR detected a higher number of positive cattle than conventional PCR in an enzootically stable area, but did not differ significantly from ELISA. No significant differences were detected between the three diagnostic tests with cattle from an enzootically unstable area. All animals raised on a tick-free facility were negative for B. bovis in the three tests. These results suggest that msa2c q-PCR is a useful test for the detection of B. bovis infection.
Thesagro: | Babesia Bovis; Babesiose; Sanidade Animal.
Categoria do assunto: | H Saúde e Patologia
Registro original: | Embrapa Gado de Corte (CNPGC)
Exemplares: | CNPGC - (13997-1) - UPC - AP - DD -  -  -  - DIS