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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
21/12/2018 |
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Data da última atualização: |
10/04/2024 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
PENNA, A. C. G.; OLIVEIRA, N. A. de; MENDONÇA, J. F. M. de; SIAS, G. R. F. V.; SOUZA, G. N. de. |
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Afiliação: |
Anna Carolina Gonçalvez Penna, Universidade Presidente Antônio Carlos/Juiz de Fora; Naiara Aparecida de Oliveira, UFJF; Juliana França Monteiro de Mendonça, UFF; Gabriel Raposo Frauches Vieira Sias, UFJF; GUILHERME NUNES DE SOUZA, CNPGL. |
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Título: |
Prevalência de Staphylococcus aureus e Streptococcus agalactiae em rebanhos leiteiros localizados na Zona da Mata de Minas Gerais. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Acta Scientiae Veterinariae, v. 46, s55, 2018. Supl. 2. |
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Idioma: |
Português |
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Notas: |
Edição dos resumos do Encontro Nacional de Epidemiologia Veterinária, Porto Alegre, 2018. |
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Palavras-Chave: |
Prevalência; Rebanhos bovinos leiteiros; Vacas infectadas. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1102414/1/Prevalencia-de-Staphylococcus-aureus.pdf
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Marc: |
LEADER 00757nam a2200193 a 4500
001 2102414
005 2024-04-10
008 2018 bl uuuu u00u1 u #d
100 1 $aPENNA, A. C. G.
245 $aPrevalência de Staphylococcus aureus e Streptococcus agalactiae em rebanhos leiteiros localizados na Zona da Mata de Minas Gerais.$h[electronic resource]
260 $aActa Scientiae Veterinariae, v. 46, s55, 2018. Supl. 2.$c2018
500 $aEdição dos resumos do Encontro Nacional de Epidemiologia Veterinária, Porto Alegre, 2018.
653 $aPrevalência
653 $aRebanhos bovinos leiteiros
653 $aVacas infectadas
700 1 $aOLIVEIRA, N. A. de
700 1 $aMENDONÇA, J. F. M. de
700 1 $aSIAS, G. R. F. V.
700 1 $aSOUZA, G. N. de
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
17/12/2015 |
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Data da última atualização: |
05/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
KUMA, K. M.; LOPES-CAITAR, V. S.; ROMERO, C. C. T.; SILVA, S. M. H.; CARVALHO, M. C. C. G.; ABDELNOOR, R. V.; DIAS, W. P.; MARCELINO-GUIMARÃES, F. C. |
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Afiliação: |
UEL; UEL; CNPSo; UEL; UENP; RICARDO VILELA ABDELNOOR, CNPSO; WALDIR PEREIRA DIAS, CNPSO; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO. |
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Título: |
A high efficient protocol for soybean root transformation by Agrobacterium rhizogenes and most stable reference genes for RT-qPCR analysis. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plant Cell Reports, v. 34, n. 11, p. 1987-2000, 2015. |
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ISSN: |
1432-203X |
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DOI: |
10.1007/s00299-015-1845-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Gene functional analyses are essential to the validation of results obtained from in silico and/or geneprospecting studies. Genetic transformation methods that yield tissues of transient expression quickly have been of considerable interest to researchers. Agrobacterium rhizogenes-mediated transformation methods, which are employed to generate plants with transformed roots, have proven useful for the study of stress caused by root phytopathogens via gene overexpression and/or silencing. While some protocols have been adapted to soybean plants, transformation efficiencies remain limited; thus, few viable plants are available for performing bioassays. Furthermore, mRNA analyses that employ reverse transcription quantitative polymerase chain reactions (RT-qPCR) require the use of reference genes with stable expression levels across different organs, development steps and treatments. In the present study, an A. rhizogenes-mediated soybean root transformation approach was optimized. The method delivers significantly higher transformation efficiency levels and rates of transformed plant recovery, thus enhancing studies of soybean abiotic conditions or interactions between phytopathogens, such as nematodes. A 55 % transformation efficiency was obtained following the addition of an acclimation step that involves hydroponics and different selection processes. The present study also validated the reference genes GmELF1-b and GmELF1-a as the most stable to be used in RT-qPCR analysis in composite plants, mainly under nematode infection. MenosGene functional analyses are essential to the validation of results obtained from in silico and/or geneprospecting studies. Genetic transformation methods that yield tissues of transient expression quickly have been of considerable interest to researchers. Agrobacterium rhizogenes-mediated transformation methods, which are employed to generate plants with transformed roots, have proven useful for the study of stress caused by root phytopathogens via gene overexpression and/or silencing. While some protocols have been adapted to soybean plants, transformation efficiencies remain limited; thus, few viable plants are available for performing bioassays. Furthermore, mRNA analyses that employ reverse transcription quantitative polymerase chain reactions (RT-qPCR) require the use of reference genes with stable expression levels across different organs, development steps and treatments. In the present study, an A. rhizogenes-mediated soybean root transformation approach was optimized. The method delivers significantly higher transformation efficiency levels and rates of transformed plant recovery, thus enhancing studies of soybean abiotic conditions or interactions between phytopathogens, such as nematodes. A 55 % transformation efficiency was obtained following the addition of an acclimation step that involves hydroponics and different selection processes. The present study also validated the reference genes GmELF1-b and GmELF1-a as the most stable to be used in RT-qPCR analysis i... Mostrar Tudo |
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Thesagro: |
Soja. |
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Categoria do assunto: |
-- |
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Marc: |
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001 2031887
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008 2015 bl uuuu u00u1 u #d
022 $a1432-203X
024 7 $a10.1007/s00299-015-1845-2$2DOI
100 1 $aKUMA, K. M.
245 $aA high efficient protocol for soybean root transformation by Agrobacterium rhizogenes and most stable reference genes for RT-qPCR analysis.$h[electronic resource]
260 $c2015
520 $aGene functional analyses are essential to the validation of results obtained from in silico and/or geneprospecting studies. Genetic transformation methods that yield tissues of transient expression quickly have been of considerable interest to researchers. Agrobacterium rhizogenes-mediated transformation methods, which are employed to generate plants with transformed roots, have proven useful for the study of stress caused by root phytopathogens via gene overexpression and/or silencing. While some protocols have been adapted to soybean plants, transformation efficiencies remain limited; thus, few viable plants are available for performing bioassays. Furthermore, mRNA analyses that employ reverse transcription quantitative polymerase chain reactions (RT-qPCR) require the use of reference genes with stable expression levels across different organs, development steps and treatments. In the present study, an A. rhizogenes-mediated soybean root transformation approach was optimized. The method delivers significantly higher transformation efficiency levels and rates of transformed plant recovery, thus enhancing studies of soybean abiotic conditions or interactions between phytopathogens, such as nematodes. A 55 % transformation efficiency was obtained following the addition of an acclimation step that involves hydroponics and different selection processes. The present study also validated the reference genes GmELF1-b and GmELF1-a as the most stable to be used in RT-qPCR analysis in composite plants, mainly under nematode infection.
650 $aSoja
700 1 $aLOPES-CAITAR, V. S.
700 1 $aROMERO, C. C. T.
700 1 $aSILVA, S. M. H.
700 1 $aCARVALHO, M. C. C. G.
700 1 $aABDELNOOR, R. V.
700 1 $aDIAS, W. P.
700 1 $aMARCELINO-GUIMARÃES, F. C.
773 $tPlant Cell Reports$gv. 34, n. 11, p. 1987-2000, 2015.
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