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Registro Completo |
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Biblioteca(s): |
Embrapa Café; Embrapa Unidades Centrais. |
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Data corrente: |
22/02/2011 |
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Data da última atualização: |
09/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MALUF, M. P.; SILVA, C. C. da; OLIVEIRA, M. de P. A. de; TAVARES, A. G.; SILVAROLLA, M. B.; GUERREIRO FILHO, O. |
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Afiliação: |
MIRIAN PEREZ MALUF, SAPC; CARLA CRISTINA DA SILVA, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; MICHELLE DE PAULA ABREU DE OLIVEIRA, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; ALINE GOMES TAVARES, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; MARIA BERNADETE SILVAROLLA, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; OLIVEIRO GUERREIRO FILHO, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'. |
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Título: |
Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, v. 34, n. 2, p. 802-810, 2009. |
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Idioma: |
Inglês |
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Conteúdo: |
In this work, we studied the biosynthesis of caffeine by examining the expression of genes involved in this biosynthetic pathway in coffee fruits containing normal or low levels of this substance. The amplification of gene-specific transcripts during fruit development revealed that low-caffeine fruits had a lower expression of the theobromine synthase and caffeine synthase genes and also contained an extra transcript of the caffeine synthase gene. This extra transcript contained only part of exon 1 and all of exon 3. The sequence of the mutant caffeine synthase gene revealed the substitution of isoleucine for valine in the enzyme active site that probably interfered with enzymatic activity. These findings indicate that the absence of caffeine in these mutants probably resulted from a combination of transcriptional regulation and the presence of mutations in the caffeine synthase amino acid sequence. |
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Palavras-Chave: |
Coffee; Cup quality; Differential expression; Naturally decaffeinated. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/29273/1/Altered-expression.pdf
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Marc: |
LEADER 01628naa a2200229 a 4500
001 1880373
005 2024-01-09
008 2009 bl uuuu u00u1 u #d
100 1 $aMALUF, M. P.
245 $aAltered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica.$h[electronic resource]
260 $c2009
520 $aIn this work, we studied the biosynthesis of caffeine by examining the expression of genes involved in this biosynthetic pathway in coffee fruits containing normal or low levels of this substance. The amplification of gene-specific transcripts during fruit development revealed that low-caffeine fruits had a lower expression of the theobromine synthase and caffeine synthase genes and also contained an extra transcript of the caffeine synthase gene. This extra transcript contained only part of exon 1 and all of exon 3. The sequence of the mutant caffeine synthase gene revealed the substitution of isoleucine for valine in the enzyme active site that probably interfered with enzymatic activity. These findings indicate that the absence of caffeine in these mutants probably resulted from a combination of transcriptional regulation and the presence of mutations in the caffeine synthase amino acid sequence.
653 $aCoffee
653 $aCup quality
653 $aDifferential expression
653 $aNaturally decaffeinated
700 1 $aSILVA, C. C. da
700 1 $aOLIVEIRA, M. de P. A. de
700 1 $aTAVARES, A. G.
700 1 $aSILVAROLLA, M. B.
700 1 $aGUERREIRO FILHO, O.
773 $tGenetics and Molecular Biology$gv. 34, n. 2, p. 802-810, 2009.
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Registro original: |
Embrapa Café (CNPCa) |
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| 1. |  | MALUF, M. P.; SILVA, C. C. da; OLIVEIRA, M. de P. A. de; TAVARES, A. G.; SILVAROLLA, M. B.; GUERREIRO FILHO, O. Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica. Genetics and Molecular Biology, v. 34, n. 2, p. 802-810, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Café. |
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