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| Registros recuperados : 175 | |
| 121. |  | MARQUES, D. K. S.; VARGAS, R. D.; LIMA, R. P.; SANTOS, R. A. C. P.; CALHEIROS, D. F.; OLIVEIRA, M. D. de. O ecossistema Pantanal e os recursos pesqueiros. In: MARQUES, D. K. S.; MORAES, A. S. (Ed.). Pesca e piscicultura no Pantanal: o produtor pergunta, a Embrapa responde. Brasília, DF: Embrapa Informação Tecnológica; Corumbá: Embrapa Pantanal, 2010. (Coleção 500 perguntas, 500 respostas). p. 15-26.| Biblioteca(s): Embrapa Pantanal. |
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| 122. | | CORREIA, J. R.; VILELA, M. de F.; CARRARA, A. A.; SEVILHA, A. C.; OLIVEIRA, M. D. de; SOARES, E. P.; SANO, S. M.; SANTOS, A. E. dos. Diálogos e percepções para construção de alternativas de uso sustentável da terra (2009). Planaltina, DF: Embrapa Cerrados, 2009. 1 folder.| Biblioteca(s): Embrapa Cerrados. |
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| 123. | | CORREIA, J. R.; VILELA, M. de F.; CARRARA, A. A.; SEVILHA, A. C.; OLIVEIRA, M. D. de; SOARES, E. P.; SANO, S. M.; SANTOS, A. E. dos. Diálogos e percepções para construção de alternativas de uso sustentável da terra. In: CONGRESSO BRASILEIRO DE SISTEMAS AGROFLORESTAIS, 7., 2009, Luziânia. Diálogo e integração de saberes em sistemas agroflorestais para sociedades sustentáveis: anais. [Luziânia]: Sociedade Brasileira de Sistemas Agroflorestais; [Brasília, DF]: EMATER-DF: Embrapa, 2009. 1 CD-ROM.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 124. | | CORREIA, J. R.; VILELA, M. de F.; CARRARA, A. A.; SEVILHA, A. C.; OLIVEIRA, M. D. de; SOARES, E. P.; SANO, S. M.; SANTOS, A. E. dos. Diálogos e percepções para construção de alternativas de uso sustentável da terra. In: CONGRESSO BRASILEIRO DE SISTEMAS AGROFLORESTAIS, 7., 2009, Luziânia. Diálogo e integração de saberes em sistemas agroflorestais para sociedades sustentáveis: anais. [Luziânia]: Sociedade Brasileira de Sistemas Agroflorestais; [Brasília, DF]: EMATER-DF: Embrapa, 2009. 1 CD-ROM.| Biblioteca(s): Embrapa Cerrados. |
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| 125. | | LOPES, F. S. C.; OLIVEIRA, J. V. de; OLIVEIRA, M. D. de; PEREIRA, V. S.; OLIVEIRA, J. E. de M.; KIILL, L. H. P. Plantas daninhas associadas às cochonilhas-farinhentas (Hemiptera: Pseudococcidae) em áreas de videira no semiárido brasileiro. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 25., 2014, Goiânia. Entomologia integrada à sociedade para o desenvolvimento sustentável: anais. Goiânia: Sociedade Entomológica do Brasil, 2014.| Biblioteca(s): Embrapa Semiárido. |
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| 126. | | OLIVEIRA, J. E. de M.; LOPES, F. S. C.; OLIVEIRA, J. V. de; OLIVEIRA, M. D. de; SOUZA, G. M. M. de; FERNANDES, M. H. de A. Parasitoides associados a Pseudococcídeos em agroecossistema de videira no Semiárido brasileiro. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 26.; CONGRESSO LATINO-AMERICANO DE ENTOMOLOGIA, 9., 2016, Maceió. Anais... Brasília, DF: Embrapa, 2016.| Biblioteca(s): Embrapa Semiárido. |
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| 127. | | CATELLA, A. C.; MORAES, A. S.; MARQUES, D. K. S.; NASCIMENTO, F. L.; LARA, J. A. F. de; OLIVEIRA, M. D. de; BORGHESI, R. Pesca: uma atividade estratégica para a conservação do Pantanal. Corumbá: Embrapa Pantanal, 2012. (Embrapa Pantanal. Artigo de Divulgação na Mídia, 152.) Formato eletronico. Disponível também em: Aquidauana News; Midia Max; Pantanal News; O Minuto; EcoFinanças; ECOA, ISE; TeSE.| Biblioteca(s): Embrapa Pantanal. |
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| 129. | | SANTOS, S. A.; OLIVEIRA, L. O. F. de; ARAUJO, M. T. B. D.; OLIVEIRA, M. D. de; SOARES, M. T. S. Índice de adequação do requerimento de água para bovinos em fazendas do Pantanal. In: SIMPÓSIO SOBRE RECURSOS NATURAIS E SOCIOECONÔMICOS DO PANTANAL, 6.; EVENTO DE INICIAÇÃO CIENTÍFICA DO PANTANAL, 1., 2013, Corumbá, MS. Desafios e soluções para o Pantanal: resumos. Corumbá: Embrapa Pantanal, 2013.| Biblioteca(s): Embrapa Pantanal. |
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| 131. | | PEREIRA, D.; MANSUR, M. C. D.; VOLKMER-RIBEIRO, C.; OLIVEIRA, M. D. de; SANTOS, C. P. dos; BERGONCI, P. E. A. Colonização de substrato artificial por macroinvertebrados límnicos, no delta do rio Jacuí (RS, Brasil). Revista Biotemas, v. 23, n.1, mar., 2010.| Biblioteca(s): Embrapa Pantanal. |
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| 133. | | OLIVEIRA, J. E. de M.; LOPES, F. S. C.; OLIVEIRA, J. V. DE; SOUZA, A. M. DE; OLIVEIRA, M. D. DE. Formigas associadas a cochonilhas-farinhentas em cultivos de videira no semiárido brasileiro. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 24., 2016, São Luis. Fruticultura: fruteiras nativas e sustentabilidade. São Luis, MA: SBF, 2016.| Biblioteca(s): Embrapa Semiárido. |
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| 134. | | RIBEIRO, C. C. A.; OLIVEIRA, O. N. C. de; GRASSI, M. J. S.; SAQUI, D.; PALHARES, J. C. P.; OLIVEIRA, M. D. de; SANTOS, S. A. Aplicativo para previsão de disponibilidade de água de bebedouros para dessedentação animal. In: EVENTO DE INICIAÇÃO CIENTÍFICA DO PANTANAL, 7., 2019, Corumbá. Resumos... Brasília, DF: Embrapa, 2019. p. 9.| Biblioteca(s): Embrapa Pantanal. |
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| 135. | | SILVA, E. L. V.; MOREIRA, M. M.; BARRETO, R. R.; BARROS, L. F. de; BARBOSA, D. S.; OLIVEIRA, M. D. de; CALHEIROS, D. F. Aporte de nutrientes e sedimentos para o Pantanal - Rios Cabaçal, Sepotuba e Jauru (MT). In: CONGRESSO DE ECOLOGIA DO BRASIL, 8., ECOLOGIA NO TEMPO DE MUDANÇAS GLOBAIS, ECOLOGY IN TIME OF GLOBAL CHANGES, 2007, Caxambú, MG. Anais ... Caxambú, MG, 2007. 2 p.| Biblioteca(s): Embrapa Pantanal. |
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| 136. | | OLIVEIRA, M. D. de; SANTOS, S. A.; NOGUEIRA, M.; PALHARES, J. C. P.; COMASTRI FILHO, J. A.; NOGUEIRA, E.; SALES, R. dos S.; CAMPOS, Z.; TOMAS, W. M. Captação e armazenamento de água para consumo animal durante a estação de seca na Planície Pantaneira. Corumbá: Embrapa Pantanal, 2020. 26 p. (Embrapa Pantanal. Documentos, 167).| Biblioteca(s): Embrapa Pantanal. |
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| 137. | | CALHEIROS, D. F.; JESUZ, C. R. de; CABRAL, I. L. L.; ZEILHOFER, P.; IORIS, A. R.; OLIVEIRA, M. D. de; OLIVEIRA, J. C. de. Avaliação preliminar da perda de nutrientes por escoamento superficial numa microbacia do Córrego Tenente Amaral, Jaciara, MT. In: SEMINÁRIO DA REDE AGROHIDRO, 3., WORKSHOP DO PROJETO OS IMPACTOS DA AGRICULTURA E DAS MUDANÇAS CLIMÁTICAS NOS RECURSOS HÍDRICOS, 1., 2015, Corumbá, MS. Água na agricultura : desafios frente às mudanças climáticas e de uso da terra: resumos. Brasília, DF: Embrapa, 2015. p. 21| Biblioteca(s): Embrapa Pantanal. |
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| 138. | | OLIVEIRA, M. D. de; SIMÕES, S. V. D.; ALBUQUERQUE, A. R. T. de; UCHÔA, I. C. P.; DANTAS, E. S.; SANTOS, E. D.; PORDEUS NETO, J. Avaliação da viabilidade do fluido ruminal de ovinos armazenado em temperatura ambiente. In: SIMPÓSIO INTERNACIONAL SOBRE CAPRINOS E OVINOS DE CORTE, 4.; FEIRA NACIONAL DO AGRONEGÓCIO DA CAPRINO-OVINOCULTURA DE CORTE, 3., 2009, João Pessoa. Anais... João Pessoa: EMEPA-PB, 2009. 3 f. 1 CD-ROM.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 139. | | ZEILHOFER, P.; CALHEIROS, D. F.; OLIVEIRA, M. D. de; DORES, E. F. G. de C.; LIMA, G. A. R.; FANTIN-CRUZ, I. Temporal patterns of water quality in the Pantanal floodplain and its contributing Cerrado upland rivers: implications for the interpretation of freshwater integrity. Wetlands Ecology and Management, v. 24, n. 6, p. 697-716, 2016.| Biblioteca(s): Embrapa Pantanal. |
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| 140. | | SANTOS, S. A.; MEDEIROS, S. R. de; LIMA, H. P. de; SALIS, S. M.; OLIVEIRA, M. D. de; SORIANO, B. M. A. Using indicators to assess the productivity and ecosystem services of native grasslands managed as pasturelands in the pantanal wetland, Brasil. In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FORESTRY SYSTEMS, 2., 2021. Proceedings reference... Brasília, DF: Embrapa, 2021. p. 523-527.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Pecuária Sudeste. |
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| Registros recuperados : 175 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
05/01/2011 |
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Data da última atualização: |
03/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
RIBEIRO; TOGAWA, R. C.; NESHICH, I. A. P.; MAZONI, I.; MANCINI, A. L.; MINARDI, R. C. de M.; SILVEIRA, C. H. da; JARDINE, J. G.; SANTORO, M. M.; NESHICH, G. |
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Afiliação: |
CRISTINA RIBEIRO, UFMG; ROBERTO COITI TOGAWA, CENARGEN; IZABELLA A. P. NESHICH, Estagiária/CNPTIA; IVAN MAZONI, CNPTIA; ADAUTO LUIZ MANCINI, CNPTIA; RAQUEL C. DE MELO MINARDI, UFMG; CARLOS H. DA SILVEIRA, UNIFEI; JOSE GILBERTO JARDINE, CNPTIA; MARCELO M. SANTORO, UFMG; GORAN NESHICH, CNPTIA. |
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Título: |
Analysis of binding properties and specificity through identification of the interface forming residues (IFR) for serine proteases in silico docked to different inhibitors. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
BMC Structural Biology, London, v. 10, n. 36, p. 1-16, 2010. |
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Idioma: |
Inglês |
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Notas: |
Disponível em:.Acesso em: 5 jan. 2011. |
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Conteúdo: |
Background: Enzymes belonging to the same super family of proteins in general operate on variety of substrates and are inhibited by wide selection of inhibitors. In this work our main objective was to expand the scope of studies that consider only the catalytic and binding pocket amino acids while analyzing enzyme specificity and instead, include a wider category which we have named the Interface Forming Residues (IFR). We were motivated to identify those amino acids with decreased accessibility to solvent after docking of different types of inhibitors to sub classes of serine proteases and then create a table (matrix) of all amino acid positions at the interface as well as their respective occupancies. Our goal is to establish a platform for analysis of the relationship between IFR characteristics and binding properties/specificity for bi-molecular complexes. Results: We propose a novel method for describing binding properties and delineating serine proteases specificity by compiling an exhaustive table of interface forming residues (IFR) for serine proteases and their inhibitors. Currently, the Protein Data Bank (PDB) does not contain all the data that our analysis would require. Therefore, an in silico approach was designed for building corresponding complexes The IFRs are obtained by ?rigid body docking? among 70 structurally aligned, sequence wise non-redundant, serine protease structures with 3 inhibitors: bovine pancreatic trypsin inhibitor (BPTI), ecotine and ovomucoid third domain inhibitor. The table (matrix) of all amino acid positions at the interface and their respective occupancy is created. We also developed a new computational protocol for predicting IFRs for those complexes which were not deciphered experimentally so far, achieving accuracy of at least 0.97. Conclusions: The serine proteases interfaces prefer polar (including glycine) residues (with some exceptions). Charged residues were found to be uniquely prevalent at the interfaces between the ?miscellaneous-virus? subfamily and the three inhibitors. This prompts speculation about how important this difference in IFR characteristics is for maintaining virulence of those organisms. Our work here provides a unique tool for both structure/function relationship analysis as well as a compilation of indicators detailing how the specificity of various serine proteases may have been achieved and/or could be altered. It also indicates that the interface forming residues which also determine specificity of serine protease sub-family can not be presented in a canonical way but rather as a matrix of alternative populations of amino acids occupying variety of IFR positions. MenosBackground: Enzymes belonging to the same super family of proteins in general operate on variety of substrates and are inhibited by wide selection of inhibitors. In this work our main objective was to expand the scope of studies that consider only the catalytic and binding pocket amino acids while analyzing enzyme specificity and instead, include a wider category which we have named the Interface Forming Residues (IFR). We were motivated to identify those amino acids with decreased accessibility to solvent after docking of different types of inhibitors to sub classes of serine proteases and then create a table (matrix) of all amino acid positions at the interface as well as their respective occupancies. Our goal is to establish a platform for analysis of the relationship between IFR characteristics and binding properties/specificity for bi-molecular complexes. Results: We propose a novel method for describing binding properties and delineating serine proteases specificity by compiling an exhaustive table of interface forming residues (IFR) for serine proteases and their inhibitors. Currently, the Protein Data Bank (PDB) does not contain all the data that our analysis would require. Therefore, an in silico approach was designed for building corresponding complexes The IFRs are obtained by ?rigid body docking? among 70 structurally aligned, sequence wise non-redundant, serine protease structures with 3 inhibitors: bovine pancreatic trypsin inhibitor (BPTI), ecotine and ovomuco... Mostrar Tudo |
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Palavras-Chave: |
Enzimas; Interface Forming Residues; Propriedades ligantes; Proteases. |
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Thesaurus NAL: |
Binding properties; Enzymes. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/23695/1/1472-6807-10-36.pdf
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Marc: |
LEADER 03733naa a2200313 a 4500
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100 1 $aRIBEIRO
245 $aAnalysis of binding properties and specificity through identification of the interface forming residues (IFR) for serine proteases in silico docked to different inhibitors.$h[electronic resource]
260 $c2010
500 $aDisponível em:<http://www.biomedcentral.com/1472-6807/10/36>.Acesso em: 5 jan. 2011.
520 $aBackground: Enzymes belonging to the same super family of proteins in general operate on variety of substrates and are inhibited by wide selection of inhibitors. In this work our main objective was to expand the scope of studies that consider only the catalytic and binding pocket amino acids while analyzing enzyme specificity and instead, include a wider category which we have named the Interface Forming Residues (IFR). We were motivated to identify those amino acids with decreased accessibility to solvent after docking of different types of inhibitors to sub classes of serine proteases and then create a table (matrix) of all amino acid positions at the interface as well as their respective occupancies. Our goal is to establish a platform for analysis of the relationship between IFR characteristics and binding properties/specificity for bi-molecular complexes. Results: We propose a novel method for describing binding properties and delineating serine proteases specificity by compiling an exhaustive table of interface forming residues (IFR) for serine proteases and their inhibitors. Currently, the Protein Data Bank (PDB) does not contain all the data that our analysis would require. Therefore, an in silico approach was designed for building corresponding complexes The IFRs are obtained by ?rigid body docking? among 70 structurally aligned, sequence wise non-redundant, serine protease structures with 3 inhibitors: bovine pancreatic trypsin inhibitor (BPTI), ecotine and ovomucoid third domain inhibitor. The table (matrix) of all amino acid positions at the interface and their respective occupancy is created. We also developed a new computational protocol for predicting IFRs for those complexes which were not deciphered experimentally so far, achieving accuracy of at least 0.97. Conclusions: The serine proteases interfaces prefer polar (including glycine) residues (with some exceptions). Charged residues were found to be uniquely prevalent at the interfaces between the ?miscellaneous-virus? subfamily and the three inhibitors. This prompts speculation about how important this difference in IFR characteristics is for maintaining virulence of those organisms. Our work here provides a unique tool for both structure/function relationship analysis as well as a compilation of indicators detailing how the specificity of various serine proteases may have been achieved and/or could be altered. It also indicates that the interface forming residues which also determine specificity of serine protease sub-family can not be presented in a canonical way but rather as a matrix of alternative populations of amino acids occupying variety of IFR positions.
650 $aBinding properties
650 $aEnzymes
653 $aEnzimas
653 $aInterface Forming Residues
653 $aPropriedades ligantes
653 $aProteases
700 1 $aTOGAWA, R. C.
700 1 $aNESHICH, I. A. P.
700 1 $aMAZONI, I.
700 1 $aMANCINI, A. L.
700 1 $aMINARDI, R. C. de M.
700 1 $aSILVEIRA, C. H. da
700 1 $aJARDINE, J. G.
700 1 $aSANTORO, M. M.
700 1 $aNESHICH, G.
773 $tBMC Structural Biology, London$gv. 10, n. 36, p. 1-16, 2010.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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