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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
01/11/2010 |
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Data da última atualização: |
29/06/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
BRITO, L. G.; BARBIERI, F. da S.; OLIVEIRA, M. C. de S.; GUERRERO, F. D. |
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Afiliação: |
LUCIANA GATTO BRITO, CPAF-RO; FABIO DA SILVA BARBIERI, CPAF-RO; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; F. D. GUERRERO, LIVESTOCK INSECTS RESEARCH LABORATORY/KERRVILLE-TX. |
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Título: |
Pasa assay for diagnosing pyrethroide resistance in the cattle tick populations in Rondonia. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., Campo Grande. Anais... Campo Grqande: CBPV, 2010. |
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Páginas: |
p. 125 - 126. |
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Idioma: |
Português |
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Conteúdo: |
Knockdown (kdr) resistance is caused by a reduction in the sensitivity of the arthropod nervous system to pyrethroids. Kdr resistance in field populations of cattle tick can severely limit pyrethroid usefulness in tick control programs. Early detection and characterization of kdr resistance are critical to the development of resistance management strategies. Cattle tick samples collected in Porto Velho and Presidente Medici, Rondonia were analyzed at Knipling-Bushland U.S. Livestock Insects Research Laboratory, USDA/ARS, Kerrville, TX, to verify the genotypes of these cattle tick populations. First, the populations were assessed using commercial cypermethrin solution by Adult Immersion Test (AIT) conducted at Embrapa Rondonia Animal Health Laboratory. Engorgement females ticks were exposed for 30 minutes in commercial cypermethrin solution prepared according to the manufacturer recommendations. The control group was formed with engorged females cattle ticks belonging to each of the populations tested, which were immersed in distilled water. Three groups of ten ticks were used in each treatment. Ten females by Presidente Medici population and three females by Porto Velho population immersed in commercial cypermethrin solution survived. The survived females were placed in B.O.D to obtain the postures. After the eggs hatch, the larvae were collected and immediately frozen at ultra-low temperature.Genomic DNA was isolated from individual larvae cattle tick and 30 larvae of each population tested by PASA (PCR amplification of specific alleles) assay for the presence of a specific nucleotide substitution in the sodium channel gene sequence that has been associated with kdr resistance in cattle tick. The primers, FG 221 and FG 227 (reaction 1) or FG 222 and FG 227 (reaction 2) produced diagnostic products for genotyping the kdr allele. Reaction products were visualized after 4% agarose gel electrophoresis followed by UV illumination after staining with Syber Green. The kdr allele was detected only in Presidente Medici population, which was considered a pyrethroid heterozygous (SR) population. This result was expected for this cattle tick population as there is report of resistance to pyrethroid pesticides, since the population showed a resistance factor of 18.35 for the pesticide evaluated. MenosKnockdown (kdr) resistance is caused by a reduction in the sensitivity of the arthropod nervous system to pyrethroids. Kdr resistance in field populations of cattle tick can severely limit pyrethroid usefulness in tick control programs. Early detection and characterization of kdr resistance are critical to the development of resistance management strategies. Cattle tick samples collected in Porto Velho and Presidente Medici, Rondonia were analyzed at Knipling-Bushland U.S. Livestock Insects Research Laboratory, USDA/ARS, Kerrville, TX, to verify the genotypes of these cattle tick populations. First, the populations were assessed using commercial cypermethrin solution by Adult Immersion Test (AIT) conducted at Embrapa Rondonia Animal Health Laboratory. Engorgement females ticks were exposed for 30 minutes in commercial cypermethrin solution prepared according to the manufacturer recommendations. The control group was formed with engorged females cattle ticks belonging to each of the populations tested, which were immersed in distilled water. Three groups of ten ticks were used in each treatment. Ten females by Presidente Medici population and three females by Porto Velho population immersed in commercial cypermethrin solution survived. The survived females were placed in B.O.D to obtain the postures. After the eggs hatch, the larvae were collected and immediately frozen at ultra-low temperature.Genomic DNA was isolated from individual larvae cattle tick and 30 larvae of each ... Mostrar Tudo |
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Palavras-Chave: |
Allele characterization; KDR; Pyrethroid resistance; Rondonia. |
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Thesaurus Nal: |
Rhipicephalus microplus. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 03025nam a2200217 a 4500
001 1865776
005 2023-06-29
008 2010 bl uuuu u00u1 u #d
100 1 $aBRITO, L. G.
245 $aPasa assay for diagnosing pyrethroide resistance in the cattle tick populations in Rondonia.
260 $aIn: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., Campo Grande. Anais... Campo Grqande: CBPV$c2010
300 $ap. 125 - 126.
520 $aKnockdown (kdr) resistance is caused by a reduction in the sensitivity of the arthropod nervous system to pyrethroids. Kdr resistance in field populations of cattle tick can severely limit pyrethroid usefulness in tick control programs. Early detection and characterization of kdr resistance are critical to the development of resistance management strategies. Cattle tick samples collected in Porto Velho and Presidente Medici, Rondonia were analyzed at Knipling-Bushland U.S. Livestock Insects Research Laboratory, USDA/ARS, Kerrville, TX, to verify the genotypes of these cattle tick populations. First, the populations were assessed using commercial cypermethrin solution by Adult Immersion Test (AIT) conducted at Embrapa Rondonia Animal Health Laboratory. Engorgement females ticks were exposed for 30 minutes in commercial cypermethrin solution prepared according to the manufacturer recommendations. The control group was formed with engorged females cattle ticks belonging to each of the populations tested, which were immersed in distilled water. Three groups of ten ticks were used in each treatment. Ten females by Presidente Medici population and three females by Porto Velho population immersed in commercial cypermethrin solution survived. The survived females were placed in B.O.D to obtain the postures. After the eggs hatch, the larvae were collected and immediately frozen at ultra-low temperature.Genomic DNA was isolated from individual larvae cattle tick and 30 larvae of each population tested by PASA (PCR amplification of specific alleles) assay for the presence of a specific nucleotide substitution in the sodium channel gene sequence that has been associated with kdr resistance in cattle tick. The primers, FG 221 and FG 227 (reaction 1) or FG 222 and FG 227 (reaction 2) produced diagnostic products for genotyping the kdr allele. Reaction products were visualized after 4% agarose gel electrophoresis followed by UV illumination after staining with Syber Green. The kdr allele was detected only in Presidente Medici population, which was considered a pyrethroid heterozygous (SR) population. This result was expected for this cattle tick population as there is report of resistance to pyrethroid pesticides, since the population showed a resistance factor of 18.35 for the pesticide evaluated.
650 $aRhipicephalus microplus
653 $aAllele characterization
653 $aKDR
653 $aPyrethroid resistance
653 $aRondonia
700 1 $aBARBIERI, F. da S.
700 1 $aOLIVEIRA, M. C. de S.
700 1 $aGUERRERO, F. D.
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Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 537 | |
| 143. |  | CHAGAS, A. C. de S.; OLIVEIRA, M. C. de S.; FORIM, M. R.; GIGLIOTI, R.; MIGLIATO, M. A. T. Avaliação do óleo de Neem com teor padronizado de azadirachtina - A sobre as larvas de Rhipicephalus Boophilus microplus. In: CONGRESSO BRASILEIRA DE VETERINÁRIA, 34., 2007, Santos. Resumos... São Paulo, Sociedade Paulista de Medicina Veterinária, 2007. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
|    |
| 144. | | OLIVEIRA, M. C. de S.; CHAGAS, A. C. de S.; FORIM, M.; SILVA, M. F.; GIGLIOTI, R.; CARVALHO, C. O. Ação de extrato de Neem sobre Rhipicephalus ( Boophilus micropulus) com azadirachtina - A quantificada por HPLC. In: REUNION ASOCIACION LATINOAMERICANA DE PRODUCCION ANIMAL, 20.; REUNION ASOCIACION PERUANA DE PRODUCCION ANINAL, 30.; CONGRESSO INTERNACIONAL DE GANDEIRA DE DOBLE PROPOSTO, 5., 2007, Cuzco, Perú. Annales... Cuzco: ALPA: APPA, 2007. 1 CD-ROM. Resumo na seguinte fonte: Archivos Latinoamericano de Produccion Animal, v. 15, (supl.), p. 489, 2007| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 145. | | KATIKI, L. M.; CHAGAS, A. C. de S.; OLIVEIRA, M. C. de S.; VERÍSSIMO, C. J.; FERRENZINI, J. Atividade dos óleos essenciais de mentha piperita, cymbopogon scoenanthus, cymbopogon martinii e rosmarinus officinalis sobre larvas de rhipicephalus (boophilus) microplus In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 15.; SEMINÁRIO DE PARASITOLOGIA VETERINÁRIA DOS PAÍSES DO MERCUSUL, 2., 2008, Curitiba. Anais... Curitiba: CBPV, 2008. 1 CD-ROM| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 146. | | CHAGAS, A. C. de S.; OLIVEIRA, M. C. de S.; SARRIA, A.; BELLETE, B.; CAZAL, C.; BATALHÃO, J.; FERNANDES, J. Atividade in vitro de extratos vegetais e isolados sobre larvas e fêmeas ingurgitadas de Rhipicephalus ( Boophilus) microplus. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 15.; SEMINÁRIO DE PARASITOLOGIA VETERINÁRIA DOS PAÍSES DO MERCUSUL, 2., 2008, Curitiba. Anais... Curitiba: CBPV, 2008. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 147. | | FUNES-HUACCA, M.E.; GIGLIOTI, R.; NÉO, T. A.; CARRILHO, E.; BRITO, L. G.; OLIVEIRA, M. C. de S. ANÁLISE DO PERFIL PROTÉICO DOS PRODUTOS DE EXCREÇÃO/SECREÇÃO DE LARVAS DE Cochliomyia hominivorax. In: SIMPÓSIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA PECUÁRIA SUDESTE, 2006, São Carlos, SP. Anais... São Carlos: Embrapa Pecuária Sudeste, 2006.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 148. | | PORTILHO, A. I.; GIGLIOTI, R.; OLIVEIRA, H. N. de; MARCONDES, C. R.; OKINO, C. H.; OLIVEIRA, M. C. de S. Associação entre os níveis de infecção por Babesia bovis e Babesia bigemina em amostras de sangue e carrapatos Rhipicephalus microplus colhidos em bovinos da raça Canchim. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 9., 2017, São Carlos, SP. Anais... São Carlos, SP: Embrapa Pecuária Sudeste; Embrapa Instrumentação, 2017. p. 37. (Embrapa Pecuária Sudeste. Documentos, 126). Editores técnicos: Alexandre Berndt, Ana Rita Araujo Nogueira, Bianca Baccili Zanotto Vigna, Juliana Gonçalves Costa, Lea Chapaval, Manuel Antonio Chagas Jacinto, Patricia Menezes Santos.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 151. | | BRITO, L. G.; BARBIERI, F. da S.; GIGLIOTI, R.; SOUZA, G. C. R.; BENITEZ, F.; OLIVEIRA, M. C. de S. Babesia bovis infection in catlle from Rondonia, Brazil. In: WORLD BUATRICS CONGRESS, 26., 2010, Santiago. Abstracts...Santiago: BUATRICS, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Rondônia. |
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| 152. | | SILVA, A. M. da; ALENCAR, M. M. de; OLIVEIRA, M. C. de S.; NOVAES, A. P. de; TULLIO, R. R. Correlações genéticas entre peso de machos e características reprodutivas e de crescimento de fêmeas, na raça Canchim. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 36.,1999, Porto Alegre, RS. Anais... Porto Alegre: SBZ, 1999. p. 156.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 153. | | GULIAS, A. P. S. M.; RIBEIRO, J. F.; OLIVEIRA, M. C. de; AQUINO, F. de G.; SILVA, M. R. da. Cadeia produtiva do pequi (Caryocar brasiliense cambess.) no município de Damianóplis-GO. In: SIMPÓSIO NACIONAL CERRADO, 9.; SIMPÓSIO INTERNACIONAL SAVANAS TROPICAIS, 2., 2008, Brasília, DF. Desafios e estratégias para o equilíbrio entre sociedade, agronegócio e recursos naturais: anais... Planaltina, DF: Embrapa Cerrados, 2008. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Cerrados. |
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| 154. | | OLIVEIRA, M. C. de; SILVA, C. M. M. S.; ALBUQUERQUE, S. G. de; SALVIANO, L. M. C. Comportamento de gramineas forrageiras sob pastejo intensivo com bovinos. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNICA, 19., 1982, Piracicaba. Anais... Campinas: SBZ, 1982. p. 354-355. Trabalho apresentado no 1º Simpósio Brasileiro do Trópico Sem-Árido, Olinda,PE, agosto de 1982.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 156. | | CHAGAS, A. C. de S.; SILVA, I. C.; ESTEVES, S. N.; GIGLIOTI, R.; OLIVEIRA, M. C. de S.; BROCK, I. Caracterização do grau de resistência anti-helmíntica do isolado de haemonchus contortus do rebanho ovino Santa Inês da Embrapa Pecuária Sudeste, São Carlos, SP. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., Campo Grande. Anais... Campo Grqande: CBPV, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 158. | | NAKATA, L. C.; ZAROS, L. G.; COUTINHO, L. L.; OLIVEIRA, M. C. de S.; REGITANO, L. C. de A. Quantificação da expressão de IL-2, IL-12, MCP-1 em bovinos submetidos a infestação artificial por carrapatos Boophilus microplus (Acari: Ixodidae). In: CONGRESSO BRASILEIRO DE GENÉTICA , 51., 2005, Águas de Lindóia. Lindóia: SBG, 2005. p. 104| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 160. | | GULIAS, A. P. S. M.; RIBEIRO, J. F.; OLIVEIRA, M. C. de; AQUINO, F. de G.; SILVA, M. R. da. Produtividade dos pequizeiros (Caryocar brasiliense cambess.) no município de Damianópolis, Goiás. In: SIMPÓSIO NACIONAL CERRADO, 9.; SIMPÓSIO INTERNACIONAL SAVANAS TROPICAIS, 2., 2008, Brasília, DF. Desafios e estratégias para o equilíbrio entre sociedade, agronegócio e recursos naturais: anais... Planaltina, DF: Embrapa Cerrados, 2008. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Cerrados. |
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| Registros recuperados : 537 | |
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