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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
12/12/2012 |
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Data da última atualização: |
09/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VAZ, F. A. S.; SILVA, P. A. DA; PASSOS, L. P.; HELLER, M.; MICKE, G. A.; COSTA, A. C. O.; OLIVEIRA, M. A. L. DE. |
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Afiliação: |
FERNANDO ANTONIO SIMAS VAZ, UFJF; PATRÍCIA APARECIDA DA SILVA, UFJF; LEONIDAS PAIXAO PASSOS, CNPGL; MELINA HELLER, UFSC; GUSTAVO AMADEU MICKE, UFSC; ANA CAROLINA OLIVEIRA DA COSTA, UFSC; MARCONI AUGUSTO LEAL DE OLIVEIRA, UFJF. |
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Título: |
Optimisation of a capillary zone electrophoresis methodology for simultaneous analysis of organic aliphatic acids in extractis of Brachiaria brizantha. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Phytochemical Analysis, v. 23, n. 6, p. 569-575, 2012. |
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DOI: |
https://doi.org/10.1002/pca.2355 |
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Idioma: |
Inglês |
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Conteúdo: |
Introduction: Aluminum toxicity is commonly verified in acidic soils, and poses a severe limitation to plant growth and development. Therefore, Al complexation by the root system mucilage, Al complexation by organic compounds that are exuded by the roots and internal metabolic processes must be monitored by organic acids (OA), since they play a central role in these aluminum tolerance mechanisms. Objective: To optimise a capillary zone electrophoresis method able to perform simultaneous separation of acetic, citric, formic, lactic, malic, oxalic, pyruvic, succinic, tartaric and aspartic acid in plant extract solutions. Methodology: Method optimisation was achieved by a chemometric approach through experimental designs. The optimal condition found was: 20 mmol/L phthalic acid buffer; 0.8 mmol/L cetyltrimethyl-ammonium bromide; pH 3.4 adjusted with tris(hydroxymethyl)aminomethane (around 16 mmol/L); -15 kV of voltage; 25 °C of cartridge temperature; indirect ultraviolet detection at 240 nm; and 25 mbar injection for 2 s, within an analysis time of 4 min. Results: As a repeatability test of the optimal condition, 30 replicates were carried out with the same working electrolyte, where the relative standard deviation of each peak ranged from 0.081 to 0.36% (for migration time) and from 2.4 to 4.6% (for peak area). Conclusion: The methodology was successfully applied to simultaneously determine citric, malic and aspartic acid in roots and leaves extract solutions of Brachiaria brizantha, demonstrating its usefulness to study aluminum tolerance. MenosIntroduction: Aluminum toxicity is commonly verified in acidic soils, and poses a severe limitation to plant growth and development. Therefore, Al complexation by the root system mucilage, Al complexation by organic compounds that are exuded by the roots and internal metabolic processes must be monitored by organic acids (OA), since they play a central role in these aluminum tolerance mechanisms. Objective: To optimise a capillary zone electrophoresis method able to perform simultaneous separation of acetic, citric, formic, lactic, malic, oxalic, pyruvic, succinic, tartaric and aspartic acid in plant extract solutions. Methodology: Method optimisation was achieved by a chemometric approach through experimental designs. The optimal condition found was: 20 mmol/L phthalic acid buffer; 0.8 mmol/L cetyltrimethyl-ammonium bromide; pH 3.4 adjusted with tris(hydroxymethyl)aminomethane (around 16 mmol/L); -15 kV of voltage; 25 °C of cartridge temperature; indirect ultraviolet detection at 240 nm; and 25 mbar injection for 2 s, within an analysis time of 4 min. Results: As a repeatability test of the optimal condition, 30 replicates were carried out with the same working electrolyte, where the relative standard deviation of each peak ranged from 0.081 to 0.36% (for migration time) and from 2.4 to 4.6% (for peak area). Conclusion: The methodology was successfully applied to simultaneously determine citric, malic and aspartic acid in roots and leaves extract solutions of Brachiaria bri... Mostrar Tudo |
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Palavras-Chave: |
Acidos organicos; Design fatorial; Tolerancia a aluminio; Zona capilar. |
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Thesagro: |
Brachiaria Brizantha; Eletroforese. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02457naa a2200277 a 4500
001 1942177
005 2024-02-09
008 2012 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1002/pca.2355$2DOI
100 1 $aVAZ, F. A. S.
245 $aOptimisation of a capillary zone electrophoresis methodology for simultaneous analysis of organic aliphatic acids in extractis of Brachiaria brizantha.$h[electronic resource]
260 $c2012
520 $aIntroduction: Aluminum toxicity is commonly verified in acidic soils, and poses a severe limitation to plant growth and development. Therefore, Al complexation by the root system mucilage, Al complexation by organic compounds that are exuded by the roots and internal metabolic processes must be monitored by organic acids (OA), since they play a central role in these aluminum tolerance mechanisms. Objective: To optimise a capillary zone electrophoresis method able to perform simultaneous separation of acetic, citric, formic, lactic, malic, oxalic, pyruvic, succinic, tartaric and aspartic acid in plant extract solutions. Methodology: Method optimisation was achieved by a chemometric approach through experimental designs. The optimal condition found was: 20 mmol/L phthalic acid buffer; 0.8 mmol/L cetyltrimethyl-ammonium bromide; pH 3.4 adjusted with tris(hydroxymethyl)aminomethane (around 16 mmol/L); -15 kV of voltage; 25 °C of cartridge temperature; indirect ultraviolet detection at 240 nm; and 25 mbar injection for 2 s, within an analysis time of 4 min. Results: As a repeatability test of the optimal condition, 30 replicates were carried out with the same working electrolyte, where the relative standard deviation of each peak ranged from 0.081 to 0.36% (for migration time) and from 2.4 to 4.6% (for peak area). Conclusion: The methodology was successfully applied to simultaneously determine citric, malic and aspartic acid in roots and leaves extract solutions of Brachiaria brizantha, demonstrating its usefulness to study aluminum tolerance.
650 $aBrachiaria Brizantha
650 $aEletroforese
653 $aAcidos organicos
653 $aDesign fatorial
653 $aTolerancia a aluminio
653 $aZona capilar
700 1 $aSILVA, P. A. DA
700 1 $aPASSOS, L. P.
700 1 $aHELLER, M.
700 1 $aMICKE, G. A.
700 1 $aCOSTA, A. C. O.
700 1 $aOLIVEIRA, M. A. L. DE
773 $tPhytochemical Analysis$gv. 23, n. 6, p. 569-575, 2012.
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