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Registro Completo |
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
18/03/2022 |
Data da última atualização: |
18/03/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SOARES, J. C.; SOARES, A. C.; ANGELIM, M. K. S. C.; PROENÇA-MODENA, J. L.; VIEIRA, P. M. M.; MATTOSO, L. H. C.; OLIVEIRA JR, O. N. |
Afiliação: |
LUIZ HENRIQUE CAPPARELLI MATTOSO, CNPDIA. |
Título: |
Diagnostics of SARS-CoV-2 infection using electrical impedance spectroscopy with an immunosensor to detect the spike protein. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Talanta, v. 239, 123076, 2022. |
Páginas: |
1 - 7 |
ISSN: |
0039-9140 |
DOI: |
https://doi.org/10.1016/j.talanta.2021.123076 |
Idioma: |
Inglês |
Conteúdo: |
Mass testing for the diagnostics of COVID-19 has been hampered in many countries owing to the high cost of the methodologies to detect genetic material of SARS-CoV-2. In this paper, we report on a low-cost immunosensor capable of detecting the spike protein of SARS-CoV-2, including in samples of inactivated virus. Detection is performed with electrical impedance spectroscopy using an immunosensor that contains a monolayer film of carboxymethyl chitosan as matrix, coated with an active layer of antibodies specific to the spike protein. In addition to a low limit of detection of 0.179 fg/mL within an almost linear behavior from 10− 20 g/mL to 10− 14 g/ mL, the immunosensor was highly selective. For the samples with the spike protein could be distinguished in multidimensional projection plots from samples with other biomarkers and analytes that could be interfering species for healthy and infected patients. The excellent analytical performance of the immunosensors was validated with the distinction between control samples and those containing inactivated SARS-CoV-2 at different concentrations. The mechanism behind the immunosensor performance is the specific antibody-protein interaction, as confirmed with the changes induced in C?H stretching and protein bands in polarization-modulated infrared reflection absorption spectra (PM-IRRAS). Because impedance spectroscopy measurements can be made with low-cost portable instruments, the immunosensor proposed here can be applied in point-of-care diagnostics for mass testing even in places with limited resources. MenosMass testing for the diagnostics of COVID-19 has been hampered in many countries owing to the high cost of the methodologies to detect genetic material of SARS-CoV-2. In this paper, we report on a low-cost immunosensor capable of detecting the spike protein of SARS-CoV-2, including in samples of inactivated virus. Detection is performed with electrical impedance spectroscopy using an immunosensor that contains a monolayer film of carboxymethyl chitosan as matrix, coated with an active layer of antibodies specific to the spike protein. In addition to a low limit of detection of 0.179 fg/mL within an almost linear behavior from 10− 20 g/mL to 10− 14 g/ mL, the immunosensor was highly selective. For the samples with the spike protein could be distinguished in multidimensional projection plots from samples with other biomarkers and analytes that could be interfering species for healthy and infected patients. The excellent analytical performance of the immunosensors was validated with the distinction between control samples and those containing inactivated SARS-CoV-2 at different concentrations. The mechanism behind the immunosensor performance is the specific antibody-protein interaction, as confirmed with the changes induced in C?H stretching and protein bands in polarization-modulated infrared reflection absorption spectra (PM-IRRAS). Because impedance spectroscopy measurements can be made with low-cost portable instruments, the immunosensor proposed here can be ap... Mostrar Tudo |
Palavras-Chave: |
Impedance spectroscopy; Information visualization; SARS-CoV-2; Spike protein. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02452naa a2200277 a 4500 001 2141031 005 2022-03-18 008 2022 bl uuuu u00u1 u #d 022 $a0039-9140 024 7 $ahttps://doi.org/10.1016/j.talanta.2021.123076$2DOI 100 1 $aSOARES, J. C. 245 $aDiagnostics of SARS-CoV-2 infection using electrical impedance spectroscopy with an immunosensor to detect the spike protein.$h[electronic resource] 260 $c2022 300 $a1 - 7 520 $aMass testing for the diagnostics of COVID-19 has been hampered in many countries owing to the high cost of the methodologies to detect genetic material of SARS-CoV-2. In this paper, we report on a low-cost immunosensor capable of detecting the spike protein of SARS-CoV-2, including in samples of inactivated virus. Detection is performed with electrical impedance spectroscopy using an immunosensor that contains a monolayer film of carboxymethyl chitosan as matrix, coated with an active layer of antibodies specific to the spike protein. In addition to a low limit of detection of 0.179 fg/mL within an almost linear behavior from 10− 20 g/mL to 10− 14 g/ mL, the immunosensor was highly selective. For the samples with the spike protein could be distinguished in multidimensional projection plots from samples with other biomarkers and analytes that could be interfering species for healthy and infected patients. The excellent analytical performance of the immunosensors was validated with the distinction between control samples and those containing inactivated SARS-CoV-2 at different concentrations. The mechanism behind the immunosensor performance is the specific antibody-protein interaction, as confirmed with the changes induced in C?H stretching and protein bands in polarization-modulated infrared reflection absorption spectra (PM-IRRAS). Because impedance spectroscopy measurements can be made with low-cost portable instruments, the immunosensor proposed here can be applied in point-of-care diagnostics for mass testing even in places with limited resources. 653 $aImpedance spectroscopy 653 $aInformation visualization 653 $aSARS-CoV-2 653 $aSpike protein 700 1 $aSOARES, A. C. 700 1 $aANGELIM, M. K. S. C. 700 1 $aPROENÇA-MODENA, J. L. 700 1 $aVIEIRA, P. M. M. 700 1 $aMATTOSO, L. H. C. 700 1 $aOLIVEIRA JR, O. N. 773 $tTalanta$gv. 239, 123076, 2022.
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6. | | WILSON, D. W.; IBAÑEZ, G.; MATERÓN, E.; CORREA, D. S.; OLIVEIRA JR. O. N. Magnetic nanoparticles modified with L-Lysine and Melittin as recognition system in the detection of pathogenic bacteria in food samples. In: BRAZIL MRS MEETING - SBPMAT, 17, 2018, Natal, RN. Proceedings... Natal, RN: SBPMat, 2018.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Instrumentação. |
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7. | | BORATO, C. E.; LEITE, F. L.; OLIVEIRA JR, O. N.; MATTOSO, L. H. C. Efficient taste sensors made of bare metal electrodes. Sensor Letters, University Park, Pennsylvania, v. 4, n. 2, p. 1-5, 2006.Biblioteca(s): Embrapa Instrumentação. |
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10. | | RIUL JR, A.; DHANABALAN, A.; MATTOSO, L. H. C.; SOUZA, L. M. de; TICIANELLI, E. A.; OLIVEIRA JR, O. N. Characterization of 16-mer polyaniline composite Lamgmuir-blodgett films. Thin solid films, Lausanne, v. 327/329, p. 576-580, 1998.Biblioteca(s): Embrapa Instrumentação. |
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14. | | CARVALHO, E. R.; CONSOLIN FILHO, N.; FIRMINO, A.; OLIVEIRA JR, O. N.; MATTOSO, L. H. C.; MARTIN-NETO, L. Sensorial system to detect chloroform in water. Sensor Letters, University Park, Pennsylvania, v. 4, n. 2, p. 1-6, 2006.Biblioteca(s): Embrapa Instrumentação. |
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16. | | RIUL JR. A.; WOHNRATH, K.; DI TOMMAZO, R.; CARVALHO, A. C. P. L. F.; FONSECA, F. J.; OLIVEIRA JR. O. N.; TAYLOR, D. M.; MATTOSO, L. H. C. Artificial taste sensor: efficient combination of sensors made rom langmuir-blodget films of conducting polymers and a ruthenium complex and self-assembled films of na azobenzene-containing polymer. Langmuir, Washington, v.18, n.1, p.239-245, 2001.Biblioteca(s): Embrapa Instrumentação. |
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19. | | MANZOLI, A.; MERCANTE, L. A.; SHIMIZU, F. M.; PARIS, E. C.; OLIVEIRA JR, O. N.; CORREA, D. S.; MATTOSO, L. H. C. Caracterização de filmes LBL dos nanocompósitos híbridos AGCL/PANI. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 8, 2014, Juiz de fora. Anais... São Carlos: Embrapa Instrumentação; Campo Grande: Embrapa Gado de Corte; Juiz de Fora: Embrapa Gado de Leite, 2014. p. 55-58. Editores: Maria Alice Martins, Humberto de Mello Brandão, Marlene de Barros Coelho, Daniel Souza Corrêa, Caue Ribeiro, Luiz Henrique Capparelli Mattoso.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Instrumentação. |
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20. | | SOARES, A. C.; SOARES, J. C.; RODRIGUES, V. C.; BRANDAO, H. de M.; OLIVEIRA JR, O. N.; MATTOSO, L. H. C. Biossensores microfluidicos aplicados na detecção de mastite em gados leiteiros. In: SIMPÓSIO NACIONAL DE INSTRUMENTAÇÃO AGROPECUÁRIA, 4., 2019, São Carlos, SP. Ciência, inovação e mercado: anais. São Carlos, SP: Embrapa Instrumentação, 2019. Editores: Paulino Ribeiro Villas-Boas, Maria Alice Martins, Débora Marcondes Bastos Pereira Milori, Ladislau Martin Neto. SIAGRO 2019. 651-653Tipo: Artigo de Divulgação na Mídia |
Biblioteca(s): Embrapa Instrumentação. |
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