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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
01/04/2005 |
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Data da última atualização: |
06/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LEDO, F. J. da S.; PEREIRA, A. V.; BOTREL, M. de A.; SOUZA SOBRINHO, F. de; OLIVEIRA, J. S. e; XAVIER, D. F.; HEINEMANN, A. B.; FERREIRA, R. de P. |
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Afiliação: |
FRANCISCO JOSE DA SILVA LEDO, CNPGL; ANTONIO VANDER PEREIRA, CNPGL; MILTON DE ANDRADE BOTREL, CNPGL; FAUSTO DE SOUZA SOBRINHO, CNPGL; JACKSON SILVA E OLIVEIRA, CNPGL; DEISE FERREIRA XAVIER, CNPGL; ALEXANDRE BRYAN HEINEMANN, CNPGL; REINALDO DE PAULA FERREIRA, CNPGL. |
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Título: |
Avaliação de cultivares de alfafa na Zona da Mata de Minas Gerais. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Ciência Agrotecnologia, v.28, n.5, p.1151-1159, set./out. 2004. |
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Idioma: |
Português |
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Conteúdo: |
Realizou-se este trabalho com o objetivo de avaliar e selecionar cultivares de alfafa (Medicago sativa L.) mais adapta-das às condições edafoclimáticas da Zona da Mata de Minas Gerais. O ensaio foi conduzido no campo experimental da Em-brapa Gado de Leite, em Coronel Pacheco-MG, no delineamento experimental de blocos casualizados com duas repetições. Fo-ram avaliadas 59 cultivares no período de 31/8/1999 a 10/12/2001, realizando-se 16 cortes de avaliação. Avaliaram-se a produção de matéria seca (PMS), altura da planta (AP), vigor (VG), porcentagem de floração (FLO), relação folha/caule (RFC), e incidência de doenças (ID) e pragas (IP). Para PMS, AP, VG, FLO e ID, observaram-se diferenças significativas entre as cultivares. As cultivares P-30, Crioula C-1, Crioula C-3 e Crioula C-2 foram as que apresentaram maiores PMS (13,17; 13,13; 13,00 e 12,48 t/ha/ano, respectivamente). As cultivares apresentaram maior FLO (18,98%) e AP (54,13 cm) nos cortes realizados no período de verão, quando comparado com os de inverno (6,11% e 46,60 cm, respectivamente). Com base nos resultados obtidos, foram selecionadas as cultivares P-30, Crioula C-1, Crioula C-3 e Crioula C-2. |
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Palavras-Chave: |
Forrageira. |
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Thesagro: |
Leguminosa; Medicago Sativa; Melhoramento. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPPSE/15511/1/PROCIRPF2004.00206.PDF
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Marc: |
LEADER 01928naa a2200253 a 4500
001 1047012
005 2022-05-06
008 2004 bl uuuu u00u1 u #d
100 1 $aLEDO, F. J. da S.
245 $aAvaliação de cultivares de alfafa na Zona da Mata de Minas Gerais.$h[electronic resource]
260 $c2004
520 $aRealizou-se este trabalho com o objetivo de avaliar e selecionar cultivares de alfafa (Medicago sativa L.) mais adapta-das às condições edafoclimáticas da Zona da Mata de Minas Gerais. O ensaio foi conduzido no campo experimental da Em-brapa Gado de Leite, em Coronel Pacheco-MG, no delineamento experimental de blocos casualizados com duas repetições. Fo-ram avaliadas 59 cultivares no período de 31/8/1999 a 10/12/2001, realizando-se 16 cortes de avaliação. Avaliaram-se a produção de matéria seca (PMS), altura da planta (AP), vigor (VG), porcentagem de floração (FLO), relação folha/caule (RFC), e incidência de doenças (ID) e pragas (IP). Para PMS, AP, VG, FLO e ID, observaram-se diferenças significativas entre as cultivares. As cultivares P-30, Crioula C-1, Crioula C-3 e Crioula C-2 foram as que apresentaram maiores PMS (13,17; 13,13; 13,00 e 12,48 t/ha/ano, respectivamente). As cultivares apresentaram maior FLO (18,98%) e AP (54,13 cm) nos cortes realizados no período de verão, quando comparado com os de inverno (6,11% e 46,60 cm, respectivamente). Com base nos resultados obtidos, foram selecionadas as cultivares P-30, Crioula C-1, Crioula C-3 e Crioula C-2.
650 $aLeguminosa
650 $aMedicago Sativa
650 $aMelhoramento
653 $aForrageira
700 1 $aPEREIRA, A. V.
700 1 $aBOTREL, M. de A.
700 1 $aSOUZA SOBRINHO, F. de
700 1 $aOLIVEIRA, J. S. e
700 1 $aXAVIER, D. F.
700 1 $aHEINEMANN, A. B.
700 1 $aFERREIRA, R. de P.
773 $tCiência Agrotecnologia$gv.28, n.5, p.1151-1159, set./out. 2004.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
05/02/2014 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
OLIVEIRA, C. S.; SARAIVA, N. Z.; CRUZ, M. H. C.; MAZETI, B.; OLIVEIRA, L. Z.; LOPES, F. L.; GARCIA, J. M. |
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Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL; NAIARA ZOCCAL SARAIVA, UNESP; MARIA HELENA COELHO CRUZ, UNESP; BRUNA MAZETI, UNESP; LETICIA ZOCCOLARO OLIVEIRA, UNESP; FLAVIA LOMBARDI LOPES, UNESP; JOAQUIM MANSANO GARCIA, UNESP. |
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Título: |
HDAC inhibition decreases XIST expression on female IVP bovine blastocysts. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Reproduction, v. 145, n. 1, p. 9-17, 2013. |
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DOI: |
https://doi.org/10.1530/rep-11-0343 |
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Idioma: |
Inglês |
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Conteúdo: |
During initial development, both X chromosomes are active in females, and one of them must be silenced at the appropriate time in order to dosage compensate their gene expression levels to male counterparts. Silencing involves epigenetic mechanisms, including histone deacetylation. Major X chromosome inactivation (XCI) in bovine occurs between hatching and implantation, although in vitro culture conditions might disrupt the silencing process, increasing or decreasing X-linked gene expression. In this study, we aimed to address the roles of histone deacetylase inhibition by trichostatin A (TSA) on female preimplantation development. We tested the hypothesis that by enhancing histone acetylation, TSA would increase the percentage of embryos achieving 16-cell stage, reducing percentage of embryos blocked at 8-cell stage, and interfere with XCI in IVF embryos. We noticed that after TSA treatment, acetylation levels in individual blastomeres of 8-16 cell embryos were increased twofold on treated embryos, and the same was detected for blastocysts. Changes among blastomere levels within the same embryo were diminished on TSA group, as low-acetylated blastomeres were no longer detected. The percentage of embryos that reached the 5th cleavage cycle 118 h after IVF, analyzed by Hoechst staining, remained unaltered after TSA treatment. Then, we assessed XIST and G6PD expression in individual female bovine blastocysts by quantitative real-time PCR. Even though G6PD expression remained unaltered after TSA exposure, XIST expression was eightfold decreased, and we also detected a major decrease in the percentage of blastocysts expressing detectable XIST levels after TSA treatment. Based on these results, we conclude that HDAC is involved on XCI process in bovine embryos, and its inhibition might delay X chromosome silencing and attenuate aberrant XIST expression described for IVF embryos. MenosDuring initial development, both X chromosomes are active in females, and one of them must be silenced at the appropriate time in order to dosage compensate their gene expression levels to male counterparts. Silencing involves epigenetic mechanisms, including histone deacetylation. Major X chromosome inactivation (XCI) in bovine occurs between hatching and implantation, although in vitro culture conditions might disrupt the silencing process, increasing or decreasing X-linked gene expression. In this study, we aimed to address the roles of histone deacetylase inhibition by trichostatin A (TSA) on female preimplantation development. We tested the hypothesis that by enhancing histone acetylation, TSA would increase the percentage of embryos achieving 16-cell stage, reducing percentage of embryos blocked at 8-cell stage, and interfere with XCI in IVF embryos. We noticed that after TSA treatment, acetylation levels in individual blastomeres of 8-16 cell embryos were increased twofold on treated embryos, and the same was detected for blastocysts. Changes among blastomere levels within the same embryo were diminished on TSA group, as low-acetylated blastomeres were no longer detected. The percentage of embryos that reached the 5th cleavage cycle 118 h after IVF, analyzed by Hoechst staining, remained unaltered after TSA treatment. Then, we assessed XIST and G6PD expression in individual female bovine blastocysts by quantitative real-time PCR. Even though G6PD expression remained u... Mostrar Tudo |
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Palavras-Chave: |
Cultured Female Fertilization; Drug effects. |
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Thesaurus NAL: |
Blastocyst; Embryology; Metabolism. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02665naa a2200265 a 4500
001 1978655
005 2024-02-05
008 2013 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1530/rep-11-0343$2DOI
100 1 $aOLIVEIRA, C. S.
245 $aHDAC inhibition decreases XIST expression on female IVP bovine blastocysts.$h[electronic resource]
260 $c2013
520 $aDuring initial development, both X chromosomes are active in females, and one of them must be silenced at the appropriate time in order to dosage compensate their gene expression levels to male counterparts. Silencing involves epigenetic mechanisms, including histone deacetylation. Major X chromosome inactivation (XCI) in bovine occurs between hatching and implantation, although in vitro culture conditions might disrupt the silencing process, increasing or decreasing X-linked gene expression. In this study, we aimed to address the roles of histone deacetylase inhibition by trichostatin A (TSA) on female preimplantation development. We tested the hypothesis that by enhancing histone acetylation, TSA would increase the percentage of embryos achieving 16-cell stage, reducing percentage of embryos blocked at 8-cell stage, and interfere with XCI in IVF embryos. We noticed that after TSA treatment, acetylation levels in individual blastomeres of 8-16 cell embryos were increased twofold on treated embryos, and the same was detected for blastocysts. Changes among blastomere levels within the same embryo were diminished on TSA group, as low-acetylated blastomeres were no longer detected. The percentage of embryos that reached the 5th cleavage cycle 118 h after IVF, analyzed by Hoechst staining, remained unaltered after TSA treatment. Then, we assessed XIST and G6PD expression in individual female bovine blastocysts by quantitative real-time PCR. Even though G6PD expression remained unaltered after TSA exposure, XIST expression was eightfold decreased, and we also detected a major decrease in the percentage of blastocysts expressing detectable XIST levels after TSA treatment. Based on these results, we conclude that HDAC is involved on XCI process in bovine embryos, and its inhibition might delay X chromosome silencing and attenuate aberrant XIST expression described for IVF embryos.
650 $aBlastocyst
650 $aEmbryology
650 $aMetabolism
653 $aCultured Female Fertilization
653 $aDrug effects
700 1 $aSARAIVA, N. Z.
700 1 $aCRUZ, M. H. C.
700 1 $aMAZETI, B.
700 1 $aOLIVEIRA, L. Z.
700 1 $aLOPES, F. L.
700 1 $aGARCIA, J. M.
773 $tReproduction$gv. 145, n. 1, p. 9-17, 2013.
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