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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
08/01/2015 |
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Data da última atualização: |
22/08/2018 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
OLIVEIRA, J. A. CASTRO, I. A.; PARIS, E. C.; RIBEIRO, C.; GIRALDI, T. R.; POIRIER, G. Y. |
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Afiliação: |
ELAINE CRISTINA PARIS, CNPDIA; CAUE RIBEIRO DE OLIVEIRA, CNPDIA. |
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Título: |
Heteroestruturas de WO3:TiO2 aplicadas na fotodegradação de corantes. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 8, 2014, Juiz de fora. Anais... São Carlos: Embrapa Instrumentação; Campo Grande: Embrapa Gado de Corte; Juiz de Fora: Embrapa Gado de Leite, 2014. p. 355-359. Editores: Maria Alice Martins, Humberto de Mello Brandão, Marlene de Barros Coelho, Daniel Souza Corrêa, Caue Ribeiro, Luiz Henrique Capparelli Mattoso. |
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ISSN: |
2175-8395 |
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Idioma: |
Português |
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Palavras-Chave: |
Aplicações no agronegócio; Evento; Novos materiais; Processos em nanotecnologia. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/115126/1/arq-45.pdf
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Marc: |
LEADER 00951nam a2200205 a 4500
001 2004815
005 2018-08-22
008 2014 bl uuuu u00u1 u #d
022 $a2175-8395
100 1 $aOLIVEIRA, J. A. CASTRO, I. A.
245 $aHeteroestruturas de WO3$bTiO2 aplicadas na fotodegradação de corantes.
260 $aIn: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 8, 2014, Juiz de fora. Anais... São Carlos: Embrapa Instrumentação; Campo Grande: Embrapa Gado de Corte; Juiz de Fora: Embrapa Gado de Leite, 2014. p. 355-359. Editores: Maria Alice Martins, Humberto de Mello Brandão, Marlene de Barros Coelho, Daniel Souza Corrêa, Caue Ribeiro, Luiz Henrique Capparelli Mattoso.$c2014
653 $aAplicações no agronegócio
653 $aEvento
653 $aNovos materiais
653 $aProcessos em nanotecnologia
700 1 $aPARIS, E. C.
700 1 $aRIBEIRO, C.
700 1 $aGIRALDI, T. R.
700 1 $aPOIRIER, G. Y.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
31/05/2016 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
ELEOTÉRIO, R. B.; SEPÚLVEDA, R. V.; REIS, E. C. C.; VALENTE, F. L.; BORGES, A P. B. |
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Afiliação: |
RENATO B. ELEOTÉRIO, UFV; RODRIGO V. SEPULVEDA, UFV; EMILY C. C. REIS, UFV; FABRÍCIO L. VALENTE, UFV; ANDRÉA P. B. BORGES, UFV. |
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Título: |
Isolation, expansion and differentiation of mesenchymal stromal cells from rabbits' marrow'. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 5, p. 423-430, maio. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Tissue engineering has been a fundamental technique in the regenerative medicine field, once it permits to build tri-dimensional tissue constructs associating undifferentiated mesenchymal cells (or mesenchymal stromal cells - MSCs) and scaffolds in vitro. Therefore, many studies have been carried out using these cells from different animal species, and rabbits are often used as animal model for in vivo tissue repair studies. However, most of the information available about MSCs harvesting and characterization is about human and murine cells, which brings some doubts to researchers who desire to work with a rabbit model in tissue repair studies based on MSCs. In this context, this study aimed to add and improve the information available in the scientific literature providing a complete technique for isolation, expansion and differentiation of MSCs from rabbits. Bone marrow mononuclear cells (BMMCs) from humerus and femur of rabbits were obtained and to evaluate their proliferation rate, three different culture media were tested, here referred as DMEM-P, DMEM´S and ?-MEM. The BMMCs were also cultured in osteogenic, chondrogenic and adipogenic induction media to prove their multipotentiality. It was concluded that the techniques suggested in this study can provide a guideline to harvest and isolate MSCs from bone marrow of rabbits in enough amount to allow their expansion and, based on the laboratory experience where the study was developed, it is also suggested a culture media formulation to provide a better cell proliferation rate with multipotentiality preservation. MenosTissue engineering has been a fundamental technique in the regenerative medicine field, once it permits to build tri-dimensional tissue constructs associating undifferentiated mesenchymal cells (or mesenchymal stromal cells - MSCs) and scaffolds in vitro. Therefore, many studies have been carried out using these cells from different animal species, and rabbits are often used as animal model for in vivo tissue repair studies. However, most of the information available about MSCs harvesting and characterization is about human and murine cells, which brings some doubts to researchers who desire to work with a rabbit model in tissue repair studies based on MSCs. In this context, this study aimed to add and improve the information available in the scientific literature providing a complete technique for isolation, expansion and differentiation of MSCs from rabbits. Bone marrow mononuclear cells (BMMCs) from humerus and femur of rabbits were obtained and to evaluate their proliferation rate, three different culture media were tested, here referred as DMEM-P, DMEM´S and ?-MEM. The BMMCs were also cultured in osteogenic, chondrogenic and adipogenic induction media to prove their multipotentiality. It was concluded that the techniques suggested in this study can provide a guideline to harvest and isolate MSCs from bone marrow of rabbits in enough amount to allow their expansion and, based on the laboratory experience where the study was developed, it is also suggested a culture media... Mostrar Tudo |
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Palavras-Chave: |
Bone marrow stem cells; Cell therapy; Células tronco mesenquimais; Engenharia de tecido; Medicina regenerativa; Mesenchymal stromal cells; Regenerative medicine; Terapia celular. |
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Thesagro: |
Coelho; Medula osséa. |
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Thesaurus NAL: |
Rabbits; Tissue engineering. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/143602/1/Isolation-expansion.pdf
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Marc: |
LEADER 02539naa a2200313 a 4500
001 2045981
005 2017-12-29
008 2016 bl uuuu u00u1 u #d
100 1 $aELEOTÉRIO, R. B.
245 $aIsolation, expansion and differentiation of mesenchymal stromal cells from rabbits' marrow'.
260 $c2016
520 $aTissue engineering has been a fundamental technique in the regenerative medicine field, once it permits to build tri-dimensional tissue constructs associating undifferentiated mesenchymal cells (or mesenchymal stromal cells - MSCs) and scaffolds in vitro. Therefore, many studies have been carried out using these cells from different animal species, and rabbits are often used as animal model for in vivo tissue repair studies. However, most of the information available about MSCs harvesting and characterization is about human and murine cells, which brings some doubts to researchers who desire to work with a rabbit model in tissue repair studies based on MSCs. In this context, this study aimed to add and improve the information available in the scientific literature providing a complete technique for isolation, expansion and differentiation of MSCs from rabbits. Bone marrow mononuclear cells (BMMCs) from humerus and femur of rabbits were obtained and to evaluate their proliferation rate, three different culture media were tested, here referred as DMEM-P, DMEM´S and ?-MEM. The BMMCs were also cultured in osteogenic, chondrogenic and adipogenic induction media to prove their multipotentiality. It was concluded that the techniques suggested in this study can provide a guideline to harvest and isolate MSCs from bone marrow of rabbits in enough amount to allow their expansion and, based on the laboratory experience where the study was developed, it is also suggested a culture media formulation to provide a better cell proliferation rate with multipotentiality preservation.
650 $aRabbits
650 $aTissue engineering
650 $aCoelho
650 $aMedula osséa
653 $aBone marrow stem cells
653 $aCell therapy
653 $aCélulas tronco mesenquimais
653 $aEngenharia de tecido
653 $aMedicina regenerativa
653 $aMesenchymal stromal cells
653 $aRegenerative medicine
653 $aTerapia celular
700 1 $aSEPÚLVEDA, R. V.
700 1 $aREIS, E. C. C.
700 1 $aVALENTE, F. L.
700 1 $aBORGES, A P. B.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 5, p. 423-430, maio. 2016.
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Embrapa Unidades Centrais (AI-SEDE) |
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