Data/hora: 27/05/2024 17:53:31

Biblioteca(s): 	Embrapa Arroz e Feijão.	
Data corrente: 	05/06/2018	
Data da última atualização: 	30/11/2018	
Autoria: 	ABREU, F. R. M.; DEDICOVA, B.; VIANELLO, R. P.; LANNA, A. C.; OLIVEIRA, J. A. V. de; VIEIRA, A. F.; MORAIS, O. P.; MENDONÇA, J. A.; BRONDANI, C.	
Afiliação: 	FERNANDA RAQUEL MARTINS ABREU, UFG; BEATA DEDICOVA, CIAT; ROSANA PEREIRA VIANELLO, CNPAF; ANNA CRISTINA LANNA, CNPAF; JOAO AUGUSTO VIEIRA DE OLIVEIRA, UFG; ARIADNA FARIA VIEIRA, UFG; ODILON PEIXOTO MORAIS; JOAO ANTONIO MENDONCA, CNPAF; CLAUDIO BRONDANI, CNPAF.	
Título: 	Overexpression of a phospholipase (OsPLDa1) for drought tolerance in upland rice (Oryza sativa L.).	
Ano de publicação: 	2018	
Fonte/Imprenta: 	Protoplasma, v. 255, n. 6, p. 1751-1761, Nov. 2018.	
ISSN: 	1615-6102	
DOI: 	10.1007/s00709-018-1265-6	
Idioma: 	Inglês	
Conteúdo: 	This work aimed to evaluate the drought tolerance of transformed plants of the cultivar BRSMG Curinga that overexpress the rice phospholipase D &1(OsPLD&1) gene. The productivity of independent transformation event plants of the OsPLD&1 gene was evaluated in an experiment where 19 days of water deficit were applied at the reproductive stage, a very strict growing condition for upland rice. The non-genetically modified cultivar (NGM) under drought treatment reduced productivity by 89% compared with that under irrigated treatment, whereas transformed plants (PLD&1_E2) reduced productivity by only 41%. After the drought treatment, the PLD&1_E2 plants productivity was five times greater than that of the NGM plant. Moreover, no adverse effects on growth and development of the transgenic plants were observed. Seven days after the resumption of irrigation, PLD&1_E2 plants had higher stomatal conductance, greater photosynthetic rate, and transpiration rate than did NGM plants, as well as a higher expression level of the OsPLD&1 gene. A delay in the senescence process was observed in these PLD&1_E2 plants, and this was determined for the recovery of photosynthesis, with greater expression of the Rubisco and lower expression of the SOD. This finding was suggestive of decreased oxidative stress, probably due to gas exchange by the partial closure of the stomata of these transformed plants, which prevented the formation of reactive oxygen species. OsPLD&1 gene overexpression resulted in a reduction in production loss under severe water deficit and revealed a possibility for the development of upland rice cultivars that are more tolerant to extreme drought conditions.	
Thesagro: 	Arroz; Engenharia Genética; Oryza Sativa; Resistência a Seca; Stress.	
Thesaurus Nal: 	Abiotic stress; Drought tolerance; Gene expression; Genetic engineering; Phospholipases; Rice.	
Categoria do assunto: 	X Pesquisa, Tecnologia e Engenharia	
Registro original: 	Embrapa Arroz e Feijão (CNPAF)	
Exemplares: 	CNPAF - (35216-1) - UPC - AP - DD - 2018 -  - 2018 - DIS