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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
21/11/2012 |
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Data da última atualização: |
14/04/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PROTASIO, A. V.; TSAI, I. J.; BABBAGE, A.; NICHOL, S.; HUNT, M.; ASLETT, M. A.; SILVA, N. de; VELARDE, G. S.; ANDERSON, T. J. C.; CLARK, R. C.; DAVIDSON, C.; DILLON, G. P.; HOLROYD, N. E.; LOVERDE, P. T.; LLOYD, C.; MCQUILLAN, J.; OLIVEIRA, G.; OTTO, T. D.; PARKER-MANUEL, S. J.; QUAIL, M. A.; WILSON, R. A.; ZERLOTINI, A.; DUNNE, D. W.; BERRIMAN, M. |
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Afiliação: |
ANNA V. PROTASIO, Wellcome Trust Sanger Institute; ISHENG J. TSAI, Wellcome Trust Sanger Institute; ANNE BABBAGE, Wellcome Trust Sanger Institute; SARAH NICHOL, Wellcome Trust Sanger Institute; MARTIN HUNT, Wellcome Trust Sanger Institute; MARTIN A. ASLETT, Wellcome Trust Sanger Institute; NISHADI DE SILVA, Wellcome Trust Sanger Institute; GILES S. VELARDE, Wellcome Trust Sanger Institute; TIM J. C. ANDERSON, Texas Biomedical Research Institute; RICHARD C. CLARK, Wellcome Trust Sanger Institute; CLAIRE DAVIDSON, Wellcome Trust Sanger Institute; GARY P. DILLON, Wellcome Trust Sanger Institute; NANCY E. HOLROYD, Wellcome Trust Sanger Institute; PHILIP T. LOVERDE, University of Texas Health Science Center; CHRISTINE LLOYD, Wellcome Trust Sanger Institute; JACQUELLINE MCQUILLAN, Wellcome Trust Sanger Institute; GUILHERME OLIVEIRA, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz; THOMAS D. OTTO, Wellcome Trust Sanger Institute; SOPHIA J. PARKER-MANUEL, University of York; MICHAEL A. QUAIL, Wellcome Trust Sanger Institute; R. ALAN WILSON, University of York; ADHEMAR ZERLOTINI NETO, CNPTIA; DAVID W. DUNNE, University of Cambridge; MATTHEW BERRIMAN, Wellcome Trust Sanger Institute. |
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Título: |
A systematically improved high quality genome and transcriptome of the human blood fluke Schistosoma mansoni. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
PLOS Neglected Tropical Diseases, v. 6, n. 1, p. 1-13, Jan. 2012. |
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DOI: |
10.1371/journal.pntd.0001455 |
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Idioma: |
Inglês |
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Conteúdo: |
Schistosomiasis is one of the most prevalent parasitic diseases, affecting millions of people in developing countries. Amongst the human-infective species, Schistosoma mansoni is also the most commonly used in the laboratory and here we present the systematic improvement of its draft genome. We used Sanger capillary and deep-coverage Illumina sequencing from clonal worms to upgrade the highly fragmented draft 380 Mb genome to one with only 885 scaffolds and more than 81% of the bases organised into chromosomes. We have also used transcriptome sequencing (RNA-seq) from four time points in the parasite?s life cycle to refine gene predictions and profile their expression. More than 45% of predicted genes have been extensively modified and the total number has been reduced from 11,807 to 10,852. Using the new version of the genome, we identified trans-splicing events occurring in at least 11% of genes and identified clear cases where it is used to resolve polycistronic transcripts. We have produced a high-resolution map of temporal changes in expression for 9,535 genes, covering an unprecedented dynamic range for this organism. All of these data have been consolidated into a searchable format within the GeneDB (www.genedb.org) and SchistoDB (www.schistodb.net) databases. With further transcriptional profiling and genome sequencing increasingly accessible, the upgraded genome will form a fundamental dataset to underpin further advances in schistosome research. |
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Thesaurus Nal: |
Schistosoma. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/70531/1/journal.pntd.0001455.pdf
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Marc: |
LEADER 02660naa a2200421 a 4500
001 1940202
005 2020-04-14
008 2012 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pntd.0001455$2DOI
100 1 $aPROTASIO, A. V.
245 $aA systematically improved high quality genome and transcriptome of the human blood fluke Schistosoma mansoni.$h[electronic resource]
260 $c2012
520 $aSchistosomiasis is one of the most prevalent parasitic diseases, affecting millions of people in developing countries. Amongst the human-infective species, Schistosoma mansoni is also the most commonly used in the laboratory and here we present the systematic improvement of its draft genome. We used Sanger capillary and deep-coverage Illumina sequencing from clonal worms to upgrade the highly fragmented draft 380 Mb genome to one with only 885 scaffolds and more than 81% of the bases organised into chromosomes. We have also used transcriptome sequencing (RNA-seq) from four time points in the parasite?s life cycle to refine gene predictions and profile their expression. More than 45% of predicted genes have been extensively modified and the total number has been reduced from 11,807 to 10,852. Using the new version of the genome, we identified trans-splicing events occurring in at least 11% of genes and identified clear cases where it is used to resolve polycistronic transcripts. We have produced a high-resolution map of temporal changes in expression for 9,535 genes, covering an unprecedented dynamic range for this organism. All of these data have been consolidated into a searchable format within the GeneDB (www.genedb.org) and SchistoDB (www.schistodb.net) databases. With further transcriptional profiling and genome sequencing increasingly accessible, the upgraded genome will form a fundamental dataset to underpin further advances in schistosome research.
650 $aSchistosoma
700 1 $aTSAI, I. J.
700 1 $aBABBAGE, A.
700 1 $aNICHOL, S.
700 1 $aHUNT, M.
700 1 $aASLETT, M. A.
700 1 $aSILVA, N. de
700 1 $aVELARDE, G. S.
700 1 $aANDERSON, T. J. C.
700 1 $aCLARK, R. C.
700 1 $aDAVIDSON, C.
700 1 $aDILLON, G. P.
700 1 $aHOLROYD, N. E.
700 1 $aLOVERDE, P. T.
700 1 $aLLOYD, C.
700 1 $aMCQUILLAN, J.
700 1 $aOLIVEIRA, G.
700 1 $aOTTO, T. D.
700 1 $aPARKER-MANUEL, S. J.
700 1 $aQUAIL, M. A.
700 1 $aWILSON, R. A.
700 1 $aZERLOTINI, A.
700 1 $aDUNNE, D. W.
700 1 $aBERRIMAN, M.
773 $tPLOS Neglected Tropical Diseases$gv. 6, n. 1, p. 1-13, Jan. 2012.
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Embrapa Agricultura Digital (CNPTIA) |
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