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 | Acesso ao texto completo restrito à biblioteca da Embrapa Clima Temperado. Para informações adicionais entre em contato com cpact.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Clima Temperado. |
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Data corrente: |
11/03/2021 |
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Data da última atualização: |
11/03/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GARCIA, N.; DA-SILVA, C. J.; COCCO, K. L. T.; POMAGUALLI, D.; OLIVEIRA, F. K. DE; SILVA, J. V. L. DA; OLIVEIRA, A. C. B. de; AMARANTE, L. DO. |
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Afiliação: |
NATÁLIA GARCIA, UFPEL; CRISTIANE JOVELINA DA-SILVA, UFPEL; KASSIA LUIZA TEIXEIRA COCCO, UFPEL; DARWIN POMAGUALLI, UFPEL; FABIANE KLETKE DE OLIVEIRA, UFPEL; JOÃO VICTOR LEMOS DA SILVA, UFPEL; ANA CLAUDIA BARNECHE DE OLIVEIRA, CPACT; LUCIANO DO AMARANTE, UFPEL. |
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Título: |
Waterlogging tolerance of five soybean genotypes through different physiological and biochemical mechanisms. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Environmental and Experimental Botany, v. 172, 103975, 2020. |
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Páginas: |
8 p. |
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ISSN: |
0098-8472 |
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Idioma: |
Inglês |
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Conteúdo: |
Waterlogging is a serious environmental threat that limits crop growth and yield in low-lying, rainfed areas many regions across the globe. Here we investigated the effects of waterlogging and subsequent re-oxygenation on the physiology and biochemistry of three soybean [Glycine max (L.) Merrill] genotypes (PELBR10-6000, PELBR11-6028, and PELBR11-6042) and two cultivars (TEC IRGA 6070 and BMX Potência). Plants were grown under greenhouse conditions until the V4 stage when they were subjected to waterlogging for seven days. The water was then drained and plants were allowed to recover for another seven days. Overall, all genotypes suppressed waterlogging stress with distinct mechanisms. Waterlogged PELBR10-6000 surpassed control plant levels of CO2 assimilation rate and readily responded to the energy lack induced by hypoxia by activating the fermentative enzymes and alanine aminotransferase. Similar mechanisms were observed in BMX Potência, which restored metabolism to control levels at the end of the recovery. PELBR11-6028 and PELBR11-6042 activated the antioxidant defenses, and TEC IRGA 6070 did not delay flowering. |
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Thesagro: |
Água; Glycine Max; Manejo de Água; Stress. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01936naa a2200277 a 4500
001 2130627
005 2021-03-11
008 2020 bl uuuu u00u1 u #d
022 $a0098-8472
100 1 $aGARCIA, N.
245 $aWaterlogging tolerance of five soybean genotypes through different physiological and biochemical mechanisms.$h[electronic resource]
260 $c2020
300 $a8 p.
520 $aWaterlogging is a serious environmental threat that limits crop growth and yield in low-lying, rainfed areas many regions across the globe. Here we investigated the effects of waterlogging and subsequent re-oxygenation on the physiology and biochemistry of three soybean [Glycine max (L.) Merrill] genotypes (PELBR10-6000, PELBR11-6028, and PELBR11-6042) and two cultivars (TEC IRGA 6070 and BMX Potência). Plants were grown under greenhouse conditions until the V4 stage when they were subjected to waterlogging for seven days. The water was then drained and plants were allowed to recover for another seven days. Overall, all genotypes suppressed waterlogging stress with distinct mechanisms. Waterlogged PELBR10-6000 surpassed control plant levels of CO2 assimilation rate and readily responded to the energy lack induced by hypoxia by activating the fermentative enzymes and alanine aminotransferase. Similar mechanisms were observed in BMX Potência, which restored metabolism to control levels at the end of the recovery. PELBR11-6028 and PELBR11-6042 activated the antioxidant defenses, and TEC IRGA 6070 did not delay flowering.
650 $aÁgua
650 $aGlycine Max
650 $aManejo de Água
650 $aStress
700 1 $aDA-SILVA, C. J.
700 1 $aCOCCO, K. L. T.
700 1 $aPOMAGUALLI, D.
700 1 $aOLIVEIRA, F. K. DE
700 1 $aSILVA, J. V. L. DA
700 1 $aOLIVEIRA, A. C. B. de
700 1 $aAMARANTE, L. DO
773 $tEnvironmental and Experimental Botany$gv. 172, 103975, 2020.
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Registro original: |
Embrapa Clima Temperado (CPACT) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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| Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
30/09/2014 |
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Data da última atualização: |
26/02/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
VENTURELLI, G. L.; BROD, F. C. A.; ROSSI, G. B.; ZIMMERMANN, N. F.; OLIVEIRA, J. P.; FARIA, J. C.; ARISI, A. C. M. |
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Afiliação: |
GUSTAVO L. VENTURELLI, UNIVERSIDADE FEDERAL DE SANTA CATARINA; FÁBIO C. A. BROD, UNIVERSIDADE FEDERAL DE SANTA CATARINA; GABRIELA B. ROSSI, UNIVERSIDADE FEDERAL DE SANTA CATARINA; NAIRA F. ZIMMERMANN, UNIVERSIDADE FEDERAL DE SANTA CATARINA; JAISON PEREIRA DE OLIVEIRA, CNPAF; JOSIAS CORREA DE FARIA, CNPAF; ANA C. M. ARISI, UNIVERSIDADE FEDERAL DE SANTA CATARINA. |
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Título: |
A specific endogenous reference for genetically modified common bean (Phaseolus vulgaris L.) DNA quantification by real-time PCR targeting lectin gene. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Molecular Biotechnology, Totowa, v. 56, n. 11, p. 1060-1068, Nov. 2014. |
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DOI: |
10.1007/s12033-014-9786-5 |
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Idioma: |
Inglês |
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Conteúdo: |
The Embrapa 5.1 genetically modified (GM) common bean was approved for commercialization in Brazil. Methods for the quantification of this new genetically modified organism (GMO) are necessary. The development of a suitable endogenous reference is essential for GMO quantification by real-time PCR. Based on this, a new taxon-specific endogenous reference quantification assay was developed for Phaseolus vulgaris L. Three genes encoding common bean proteins (phaseolin, arcelin, and lectin) were selected as candidates for endogenous reference. Primers targeting these candidate genes were designed and the detection was evaluated using the SYBR Green chemistry. The assay targeting lectin gene showed higher specificity than the remaining assays, and a hydrolysis probe was then designed. This assay showed high specificity for 50 common bean samples from two gene pools, Andean and Mesoamerican. For GM common bean varieties, the results were similar to those obtained for non-GM isogenic varieties with PCR efficiency values ranging from 92 to 101 %. Moreover, this assay presented a limit of detection of ten haploid genome copies. The primers and probe developed in this work are suitable to detect and quantify either GM or non-GM common bean. |
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Thesagro: |
DNA; Engenharia genética; Feijão; Organismo transgênico; Phaseolus vulgaris; Planta transgênica. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02148naa a2200277 a 4500
001 1996199
005 2015-02-26
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1007/s12033-014-9786-5$2DOI
100 1 $aVENTURELLI, G. L.
245 $aA specific endogenous reference for genetically modified common bean (Phaseolus vulgaris L.) DNA quantification by real-time PCR targeting lectin gene.$h[electronic resource]
260 $c2014
520 $aThe Embrapa 5.1 genetically modified (GM) common bean was approved for commercialization in Brazil. Methods for the quantification of this new genetically modified organism (GMO) are necessary. The development of a suitable endogenous reference is essential for GMO quantification by real-time PCR. Based on this, a new taxon-specific endogenous reference quantification assay was developed for Phaseolus vulgaris L. Three genes encoding common bean proteins (phaseolin, arcelin, and lectin) were selected as candidates for endogenous reference. Primers targeting these candidate genes were designed and the detection was evaluated using the SYBR Green chemistry. The assay targeting lectin gene showed higher specificity than the remaining assays, and a hydrolysis probe was then designed. This assay showed high specificity for 50 common bean samples from two gene pools, Andean and Mesoamerican. For GM common bean varieties, the results were similar to those obtained for non-GM isogenic varieties with PCR efficiency values ranging from 92 to 101 %. Moreover, this assay presented a limit of detection of ten haploid genome copies. The primers and probe developed in this work are suitable to detect and quantify either GM or non-GM common bean.
650 $aDNA
650 $aEngenharia genética
650 $aFeijão
650 $aOrganismo transgênico
650 $aPhaseolus vulgaris
650 $aPlanta transgênica
700 1 $aBROD, F. C. A.
700 1 $aROSSI, G. B.
700 1 $aZIMMERMANN, N. F.
700 1 $aOLIVEIRA, J. P.
700 1 $aFARIA, J. C.
700 1 $aARISI, A. C. M.
773 $tMolecular Biotechnology, Totowa$gv. 56, n. 11, p. 1060-1068, Nov. 2014.
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Embrapa Arroz e Feijão (CNPAF) |
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