| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Unidades Centrais. |
|
Data corrente: |
30/12/2016 |
|
Data da última atualização: |
29/12/2017 |
|
Autoria: |
LIMA, D. C. V.; SIQUEIRA, D. B.; SILVA JUNIOR, V. A.; NERY, L. T. B.; ALBUQUERQUE, L. C. R. de; SOUZA, D. S.; MELO, C. C. S.; OLIVEIRA, E. C. S. |
|
Afiliação: |
DÉBORA C. M. LIMA, UFRPE; DANIEL B. SIQUEIRA, Zoologico do Parque Estadual de Dois Irmãos; VALDOMIRO A. SILVA JUNIOR, UFRPE; LORENA T. B. NERY, UFRPE; LUCIANA C. RAMEH DE ALBUQUERQUE, Zoologico do Parque Estadual de Dois Irmãos; DÊNISSON S. SOUZA, Zoologico do Parque Estadual de Dois Irmãos; CIBELE C. S. MELO, Zoologico do Parque Estadual de Dois Irmãos; ERIKA C. S. OLIVEIRA, UFRPE. |
|
Título: |
Ectopic testis in coati (Nasua nasua Linnaeus, 1766). |
|
Ano de publicação: |
2016 |
|
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 10, p. 999-1004, out. 2016. |
|
Idioma: |
Inglês |
|
Conteúdo: |
This paper reports a case of unilateral extracorporeal ectopic testes in a captive coati Nasua nasua) in the State Park of Dois Irmãos Zoo, Recife/PE, Brazil. The testicle was located in the subcutaneous tissue of the inguinal region not adhered to the surrounding tissues. After bilateral orchiectomy, both testes were measured, fixed with 10% formalin buffered and embedded in paraffin for histopathological evaluation. The left testis measured 1.2 cm width by 1.7cm length, and the right one measured 1.5 cm width by 2.0 cm length. The ectopic testes had seminiferous epithelium without post-meiotic germ cell lines. The non-ectopic testis had several changes in the seminiferous epithelium that indicated degeneration. In both epididymis, the lumen did not contain sperm and the major epithelial structural alterations were more distinct in the epididymis associated to the ectopic testicle. In conclusion, the ectopic testis and epididymis had lesions compatible with testicular exposition to body temperature. Non-ectopic epididymis and testis had minor lesions but could be related to the infertility of the coati. |
|
Palavras-Chave: |
Coati; Ectopico. |
|
Thesagro: |
Animal selvagem; Histopatologia; Quati; Testículo. |
|
Thesaurus Nal: |
Nasua nasua; Procyonidae; Tachycardia; Wild animals. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/152588/1/Ectopic-testis-in-coati.pdf
|
|
Marc: |
LEADER 02017naa a2200325 a 4500
001 2059694
005 2017-12-29
008 2016 bl uuuu u00u1 u #d
100 1 $aLIMA, D. C. V.
245 $aEctopic testis in coati (Nasua nasua Linnaeus, 1766).$h[electronic resource]
260 $c2016
520 $aThis paper reports a case of unilateral extracorporeal ectopic testes in a captive coati Nasua nasua) in the State Park of Dois Irmãos Zoo, Recife/PE, Brazil. The testicle was located in the subcutaneous tissue of the inguinal region not adhered to the surrounding tissues. After bilateral orchiectomy, both testes were measured, fixed with 10% formalin buffered and embedded in paraffin for histopathological evaluation. The left testis measured 1.2 cm width by 1.7cm length, and the right one measured 1.5 cm width by 2.0 cm length. The ectopic testes had seminiferous epithelium without post-meiotic germ cell lines. The non-ectopic testis had several changes in the seminiferous epithelium that indicated degeneration. In both epididymis, the lumen did not contain sperm and the major epithelial structural alterations were more distinct in the epididymis associated to the ectopic testicle. In conclusion, the ectopic testis and epididymis had lesions compatible with testicular exposition to body temperature. Non-ectopic epididymis and testis had minor lesions but could be related to the infertility of the coati.
650 $aNasua nasua
650 $aProcyonidae
650 $aTachycardia
650 $aWild animals
650 $aAnimal selvagem
650 $aHistopatologia
650 $aQuati
650 $aTestículo
653 $aCoati
653 $aEctopico
700 1 $aSIQUEIRA, D. B.
700 1 $aSILVA JUNIOR, V. A.
700 1 $aNERY, L. T. B.
700 1 $aALBUQUERQUE, L. C. R. de
700 1 $aSOUZA, D. S.
700 1 $aMELO, C. C. S.
700 1 $aOLIVEIRA, E. C. S.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 10, p. 999-1004, out. 2016.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
|
Data corrente: |
07/12/2021 |
|
Data da última atualização: |
10/12/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
LISEI-DE-SÁ, M. e; RODRIGUES‑SILVA, P. L.; MORGANTE, C. V.; MELO, B. P. de; LOURENCO, I. T.; ARRAES, F. B. M.; SOUSA, J. P. A.; GALBIERI, R.; AMORIM, R. M. S.; LINS, C. B. J. de; MACEDO, L. L. P. de; MOREIRA, V. J.; FERREIRA, G. F.; RIBEIRO, T. P.; FRAGOSO, R. da R.; SILVA, M. C. M. da; ALMEIDA-ENGLER, J. de; SA, M. F. G. de. |
|
Afiliação: |
MARIA E LISEI-DE-SÁ, Empresa de Pesquisa Agropecuária de Minas Gerais; PAOLO L. RODRIGUES‑SILVA, UCB; CAROLINA VIANNA MORGANTE, CPATSA; BRUNO PAES DE MELO, INCT PlantStress Biotech; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; FABRICIO B. M. ARRAES, INCT PlantStress Biotech; JOÃO P. A. SOUSA, UCB; RAFAEL GALBIERI, Instituto Matogrossense do Algodão; REGINA M. S. AMORIM; CAMILA B. J. DE LINS; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; VALDEIR J. MOREIRA, UNB; GILANNA F. FERREIRA; THUANNE P. RIBEIRO, INCT PlantStress Biotech; RODRIGO DA ROCHA FRAGOSO, CPAC; MARIA CRISTINA MATTAR DA SILVA, Cenargen; JANICE DE ALMEIDA-ENGLER, UMR Institut Sophia Agrobiotech INRA/CNRS/UNS, France; MARIA FATIMA GROSSI DE SA, Cenargen. |
|
Título: |
Pyramiding dsRNAs increases phytonematode tolerance in cotton plants. |
|
Ano de publicação: |
2021 |
|
Fonte/Imprenta: |
Planta, v. 254, 2021. |
|
DOI: |
https://doi.org/10.1007/s00425-021-03776-0 |
|
Idioma: |
Inglês |
|
Notas: |
Na publicação: Isabela T. Lourenço-Tessutti; Leonardo L. P. Macedo; Rodrigo R. Fragoso; Maria C. M. Silva; Maria F. Grossi-de-Sa. |
|
Conteúdo: |
Root-knot nematodes (RKN) represent one of the most damaging plant-parasitic nematode genera worldwide. RNAi-mediated suppression of essential nematode genes provides a novel biotechnological strategy for the development of sustainable pest-control methods. Here, we used a Host Induced Gene Silencing (HIGS) approach by stacking dsRNA sequences into a T-DNA construct to target three essential RKN genes: cysteine protease (Mi-cpl), isocitrate lyase (Mi-icl), and splicing factor (Mi-sf), called dsMinc1, driven by the pUceS8.3 constitutive soybean promoter. Transgenic dsMinc1-T4 plants infected with Meloidogyne incognita showed a signifcant reduction in gall formation (57?64%) and egg masses production (58?67%), as well as in the estimated reproduction factor (60?78%), compared with the susceptible non-transgenic cultivar. Galls of the RNAi lines are smaller than the wild-type (WT) plants, whose root systems exhibited multiple welldeveloped root swellings. Transcript levels of the three RKN-targeted genes decreased 13- to 40-fold in nematodes from transgenic cotton galls, compared with those from control WT galls. Finally, the development of non-feeding males in transgenic plants was 2?6 times higher than in WT plants, indicating a stressful environment for nematode development after RKN gene silencing. Data strongly support that HIGS of essential RKN genes is an efective strategy to improve cotton plant tolerance. This study presents the frst application of dsRNA sequences to target multiple genes to promote M. incognita tolerance in cotton without phenotypic penalty in transgenic plants. MenosRoot-knot nematodes (RKN) represent one of the most damaging plant-parasitic nematode genera worldwide. RNAi-mediated suppression of essential nematode genes provides a novel biotechnological strategy for the development of sustainable pest-control methods. Here, we used a Host Induced Gene Silencing (HIGS) approach by stacking dsRNA sequences into a T-DNA construct to target three essential RKN genes: cysteine protease (Mi-cpl), isocitrate lyase (Mi-icl), and splicing factor (Mi-sf), called dsMinc1, driven by the pUceS8.3 constitutive soybean promoter. Transgenic dsMinc1-T4 plants infected with Meloidogyne incognita showed a signifcant reduction in gall formation (57?64%) and egg masses production (58?67%), as well as in the estimated reproduction factor (60?78%), compared with the susceptible non-transgenic cultivar. Galls of the RNAi lines are smaller than the wild-type (WT) plants, whose root systems exhibited multiple welldeveloped root swellings. Transcript levels of the three RKN-targeted genes decreased 13- to 40-fold in nematodes from transgenic cotton galls, compared with those from control WT galls. Finally, the development of non-feeding males in transgenic plants was 2?6 times higher than in WT plants, indicating a stressful environment for nematode development after RKN gene silencing. Data strongly support that HIGS of essential RKN genes is an efective strategy to improve cotton plant tolerance. This study presents the frst application of dsRNA sequences to t... Mostrar Tudo |
|
Palavras-Chave: |
Fitonematóide; Interfering RNA; Nematóides das galhas. |
|
Thesagro: |
Algodão; Gossypium Hirsutum; Meloidogyne Incognita; Nematóide. |
|
Thesaurus NAL: |
Gene silencing. |
|
Categoria do assunto: |
--
G Melhoramento Genético |
|
Marc: |
LEADER 02991naa a2200445 a 4500
001 2137487
005 2021-12-10
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s00425-021-03776-0$2DOI
100 1 $aLISEI-DE-SÁ, M. e
245 $aPyramiding dsRNAs increases phytonematode tolerance in cotton plants.$h[electronic resource]
260 $c2021
500 $aNa publicação: Isabela T. Lourenço-Tessutti; Leonardo L. P. Macedo; Rodrigo R. Fragoso; Maria C. M. Silva; Maria F. Grossi-de-Sa.
520 $aRoot-knot nematodes (RKN) represent one of the most damaging plant-parasitic nematode genera worldwide. RNAi-mediated suppression of essential nematode genes provides a novel biotechnological strategy for the development of sustainable pest-control methods. Here, we used a Host Induced Gene Silencing (HIGS) approach by stacking dsRNA sequences into a T-DNA construct to target three essential RKN genes: cysteine protease (Mi-cpl), isocitrate lyase (Mi-icl), and splicing factor (Mi-sf), called dsMinc1, driven by the pUceS8.3 constitutive soybean promoter. Transgenic dsMinc1-T4 plants infected with Meloidogyne incognita showed a signifcant reduction in gall formation (57?64%) and egg masses production (58?67%), as well as in the estimated reproduction factor (60?78%), compared with the susceptible non-transgenic cultivar. Galls of the RNAi lines are smaller than the wild-type (WT) plants, whose root systems exhibited multiple welldeveloped root swellings. Transcript levels of the three RKN-targeted genes decreased 13- to 40-fold in nematodes from transgenic cotton galls, compared with those from control WT galls. Finally, the development of non-feeding males in transgenic plants was 2?6 times higher than in WT plants, indicating a stressful environment for nematode development after RKN gene silencing. Data strongly support that HIGS of essential RKN genes is an efective strategy to improve cotton plant tolerance. This study presents the frst application of dsRNA sequences to target multiple genes to promote M. incognita tolerance in cotton without phenotypic penalty in transgenic plants.
650 $aGene silencing
650 $aAlgodão
650 $aGossypium Hirsutum
650 $aMeloidogyne Incognita
650 $aNematóide
653 $aFitonematóide
653 $aInterfering RNA
653 $aNematóides das galhas
700 1 $aRODRIGUES‑SILVA, P. L.
700 1 $aMORGANTE, C. V.
700 1 $aMELO, B. P. de
700 1 $aLOURENCO, I. T.
700 1 $aARRAES, F. B. M.
700 1 $aSOUSA, J. P. A.
700 1 $aGALBIERI, R.
700 1 $aAMORIM, R. M. S.
700 1 $aLINS, C. B. J. de
700 1 $aMACEDO, L. L. P. de
700 1 $aMOREIRA, V. J.
700 1 $aFERREIRA, G. F.
700 1 $aRIBEIRO, T. P.
700 1 $aFRAGOSO, R. da R.
700 1 $aSILVA, M. C. M. da
700 1 $aALMEIDA-ENGLER, J. de
700 1 $aSA, M. F. G. de
773 $tPlanta$gv. 254, 2021.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Semiárido (CPATSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
