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Registro Completo |
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
02/08/2007 |
Data da última atualização: |
09/02/2022 |
Tipo da produção científica: |
Documentos |
Autoria: |
OLIVEIRA, C. A. V. de; CORREIA, R. C.; SILVA, C. N. da; CUNHA, W. da; FERREIRA, T. V. do C. |
Afiliação: |
CARLOS ABERTO VASCONCELOS OLIVEIRA, CPATSA; REBERT COELHO CORREIA, CPATSA. |
Título: |
Zoneamento e tipificação dos sistemas agrícolas do município de Gilbués - PI. |
Ano de publicação: |
1999 |
Fonte/Imprenta: |
Petrolina: Embrapa Semi-Árido, 1999. |
Páginas: |
16 p. |
Descrição Física: |
il. |
Série: |
(Embrapa Semi-Árido. Documentos, 123) |
Idioma: |
Português |
Conteúdo: |
Características do município; Coleta de dados; Modelo estatístico; Sistemas de produção; Tipos de sistema de produção praticados pelos pequenos produtoires; Distribuição dos pequenos produtores por tipo; Estrutura da propriedade; Uso de tecnologias; Estrutura familiar e mão de obra; Estrura da renda; Estrutura hídrica. |
Palavras-Chave: |
Gilbués; Nordeste; Piauí; Recursos naturais; Sistema agrícola; Tipificação; Zoneamento. |
Thesagro: |
Pequeno Produtor; Zoneamento Agrícola. |
Thesaurus Nal: |
Agricultural zoning. |
Categoria do assunto: |
B Sociologia Rural |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPATSA/35962/1/SDC123.pdf
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Marc: |
LEADER 01199nam a2200301 a 4500 001 1158711 005 2022-02-09 008 1999 bl uuuu u0uu1 u #d 100 1 $aOLIVEIRA, C. A. V. de 245 $aZoneamento e tipificação dos sistemas agrícolas do município de Gilbués - PI.$h[electronic resource] 260 $aPetrolina: Embrapa Semi-Árido$c1999 300 $a16 p.$cil. 490 $a(Embrapa Semi-Árido. Documentos, 123) 520 $aCaracterísticas do município; Coleta de dados; Modelo estatístico; Sistemas de produção; Tipos de sistema de produção praticados pelos pequenos produtoires; Distribuição dos pequenos produtores por tipo; Estrutura da propriedade; Uso de tecnologias; Estrutura familiar e mão de obra; Estrura da renda; Estrutura hídrica. 650 $aAgricultural zoning 650 $aPequeno Produtor 650 $aZoneamento Agrícola 653 $aGilbués 653 $aNordeste 653 $aPiauí 653 $aRecursos naturais 653 $aSistema agrícola 653 $aTipificação 653 $aZoneamento 700 1 $aCORREIA, R. C. 700 1 $aSILVA, C. N. da 700 1 $aCUNHA, W. da 700 1 $aFERREIRA, T. V. do C.
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| Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
Data corrente: |
13/09/2021 |
Data da última atualização: |
15/09/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
RIBEIRO, T. P.; LOURENCO, I. T.; MELO, B. P. de; MORGANTE, C. V.; SALLES FILHO, A.; LINS, C. B. J.; FERREIRA, G. F.; MELLO, G. N.; MACEDO, L. L. P. de; LUCENA, W. A.; SILVA, M. C. M. da; OLIVEIRA‑NETO, O. B.; SA, M. F. G. de. |
Afiliação: |
THUANNE PIRES RIBEIRO, UNB; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; BRUNO PAES DE MELO, UFV; CAROLINA VIANNA MORGANTE, CPATSA; ALVARO SALLES FILHO, UCB; CAMILA BARROZO JESUS LINS; GILANNA FALCÃO FERREIRA; GLÊNIA NUNES MELLO; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; WAGNER ALEXANDRE LUCENA, Cenargen; MARIA CRISTINA MATTAR DA SILVA, Cenargen; OSMUNDO BRILHANTE OLIVEIRA‑NETO, Faculdade Planalto Central; MARIA FATIMA GROSSI DE SA, Cenargen. |
Título: |
Improved cotton transformation protocol mediated by Agrobacterium and biolistic combined-methods. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Planta, v. 254, 20, 2021. |
ISSN: |
1432-2048; 0032-0935 |
DOI: |
https://doi-org.ez103.periodicos.capes.gov.br/10.1007/s00425-021-03666-5 |
Idioma: |
Inglês |
Conteúdo: |
Cotton (Gossypium spp.) is the most important crop for natural textile fiber production worldwide. Nonetheless, one of the main challenges in cotton production are the losses resulting from insect pests, pathogens, and abiotic stresses. One effective way to solve these issues is to use genetically modified (GM) varieties. Herein, we describe an improved protocol for straightforward and cost-effective genetic transformation of cotton embryo axes, merging biolistics and Agrobacterium. The experimental steps include (1) Agrobacterium preparation, (2) seed sterilization, (3) cotton embryo excision, (4) lesion of shoot-cells by tungsten bombardment, (5) Agrobacterium-mediated transformation, (6) embryo co-culture, (7) regeneration and selection of transgenic plants in vitro, and (8) molecular characterization of plants. Due to the high regenerative power of the embryonic axis and the exceptional ability of the meristem cells for plant regeneration through organogenesis in vitro, this protocol can be performed in approximately 4?10 weeks, with an average plant regeneration of about 5.5% (±?0.53) and final average transformation efficiency of 60% (±?0.55). The transgene was stably inherited, and most transgenic plants hold a single copy of the transgene, as desirable and expected in Agrobacterium-mediated transformation. Additionally, the transgene was stably expressed over generations, and transgenic proteins could be detected at high levels in the T2 generation of GM cotton plants. The T2 progeny showed no phenotypic or productivity disparity compared to wild-type plants. Collectively, the use of cotton embryo axes and the enhanced DNA-delivery system by combining particle bombardment and Agrobacterium infection enabled efficient transgenic plant recovery, overcoming usual limitations associated with the recalcitrance of several cotton genotypes subjected to somatic embryogenesis. The improved approach states this method?s success for cotton genetic modification, allowing us to obtain GM cotton plants carrying traits, which are of fundamental relevance for the advancement of global agribusiness. MenosCotton (Gossypium spp.) is the most important crop for natural textile fiber production worldwide. Nonetheless, one of the main challenges in cotton production are the losses resulting from insect pests, pathogens, and abiotic stresses. One effective way to solve these issues is to use genetically modified (GM) varieties. Herein, we describe an improved protocol for straightforward and cost-effective genetic transformation of cotton embryo axes, merging biolistics and Agrobacterium. The experimental steps include (1) Agrobacterium preparation, (2) seed sterilization, (3) cotton embryo excision, (4) lesion of shoot-cells by tungsten bombardment, (5) Agrobacterium-mediated transformation, (6) embryo co-culture, (7) regeneration and selection of transgenic plants in vitro, and (8) molecular characterization of plants. Due to the high regenerative power of the embryonic axis and the exceptional ability of the meristem cells for plant regeneration through organogenesis in vitro, this protocol can be performed in approximately 4?10 weeks, with an average plant regeneration of about 5.5% (±?0.53) and final average transformation efficiency of 60% (±?0.55). The transgene was stably inherited, and most transgenic plants hold a single copy of the transgene, as desirable and expected in Agrobacterium-mediated transformation. Additionally, the transgene was stably expressed over generations, and transgenic proteins could be detected at high levels in the T2 generation of GM cotton plant... Mostrar Tudo |
Palavras-Chave: |
Embryonic axis; Genotype-independent transformation; Modificação genética do algodão. |
Thesagro: |
Agrobacterium Tumefaciens; Algodão; DNA; Gossypium Hirsutum; Método de Melhoramento; Planta Transgênica. |
Thesaurus NAL: |
Abiotic stress; Biolistics; Cotton; Genetic transformation; Genetically modified plants. |
Categoria do assunto: |
-- G Melhoramento Genético |
Marc: |
LEADER 03519naa a2200457 a 4500 001 2134441 005 2021-09-15 008 2021 bl uuuu u00u1 u #d 022 $a1432-2048; 0032-0935 024 7 $ahttps://doi-org.ez103.periodicos.capes.gov.br/10.1007/s00425-021-03666-5$2DOI 100 1 $aRIBEIRO, T. P. 245 $aImproved cotton transformation protocol mediated by Agrobacterium and biolistic combined-methods.$h[electronic resource] 260 $c2021 520 $aCotton (Gossypium spp.) is the most important crop for natural textile fiber production worldwide. Nonetheless, one of the main challenges in cotton production are the losses resulting from insect pests, pathogens, and abiotic stresses. One effective way to solve these issues is to use genetically modified (GM) varieties. Herein, we describe an improved protocol for straightforward and cost-effective genetic transformation of cotton embryo axes, merging biolistics and Agrobacterium. The experimental steps include (1) Agrobacterium preparation, (2) seed sterilization, (3) cotton embryo excision, (4) lesion of shoot-cells by tungsten bombardment, (5) Agrobacterium-mediated transformation, (6) embryo co-culture, (7) regeneration and selection of transgenic plants in vitro, and (8) molecular characterization of plants. Due to the high regenerative power of the embryonic axis and the exceptional ability of the meristem cells for plant regeneration through organogenesis in vitro, this protocol can be performed in approximately 4?10 weeks, with an average plant regeneration of about 5.5% (±?0.53) and final average transformation efficiency of 60% (±?0.55). The transgene was stably inherited, and most transgenic plants hold a single copy of the transgene, as desirable and expected in Agrobacterium-mediated transformation. Additionally, the transgene was stably expressed over generations, and transgenic proteins could be detected at high levels in the T2 generation of GM cotton plants. The T2 progeny showed no phenotypic or productivity disparity compared to wild-type plants. Collectively, the use of cotton embryo axes and the enhanced DNA-delivery system by combining particle bombardment and Agrobacterium infection enabled efficient transgenic plant recovery, overcoming usual limitations associated with the recalcitrance of several cotton genotypes subjected to somatic embryogenesis. The improved approach states this method?s success for cotton genetic modification, allowing us to obtain GM cotton plants carrying traits, which are of fundamental relevance for the advancement of global agribusiness. 650 $aAbiotic stress 650 $aBiolistics 650 $aCotton 650 $aGenetic transformation 650 $aGenetically modified plants 650 $aAgrobacterium Tumefaciens 650 $aAlgodão 650 $aDNA 650 $aGossypium Hirsutum 650 $aMétodo de Melhoramento 650 $aPlanta Transgênica 653 $aEmbryonic axis 653 $aGenotype-independent transformation 653 $aModificação genética do algodão 700 1 $aLOURENCO, I. T. 700 1 $aMELO, B. P. de 700 1 $aMORGANTE, C. V. 700 1 $aSALLES FILHO, A. 700 1 $aLINS, C. B. J. 700 1 $aFERREIRA, G. F. 700 1 $aMELLO, G. N. 700 1 $aMACEDO, L. L. P. de 700 1 $aLUCENA, W. A. 700 1 $aSILVA, M. C. M. da 700 1 $aOLIVEIRA‑NETO, O. B. 700 1 $aSA, M. F. G. de 773 $tPlanta$gv. 254, 20, 2021.
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