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 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
26/01/2015 |
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Data da última atualização: |
26/01/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HALLWASS, M.; OLIVEIRA, A. S. de; DIANESE, E. de C.; LOHUIS, D.; BOITEUX, L. S.; NAGATA, A. K. I.; RESENDE, R. O.; KORMELINK, R. |
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Afiliação: |
MARIANA HALLWASS, LABORATORY OF VIROLOGY, DEPATMENT OF PLANT SCIENCES, WAGENINGEN UNIVERSITY.; ATHOS SILVA DE OLIVEIRA, INSTITUTODE CIENCIAS BIOLÓGICAS, DEPARTAMENTO DE BIOLOGIA CELUALAR, UNIVERSIDADE DE BRASILIA.; ERICO DE CAMPOS DIANESE, INSTITUTO DE CIENCIAS BIOLÓGICAS, DEPARTAMENTO DE BIOLOGIA CELULAR, UNIVERSIDADE DE BRASÍLIA.; DICK LOHUIS, INSTITUTO DE CIENCIAS BIOLÓGICAS, DEPARTAMENTO DE BIOLOGIA CELULAR, UNIVERSIDADE DE BRASÍLIA.; LEONARDO SILVA BOITEUX, CNPH; ALICE KAZUKO INOUE NAGATA, CNPH; RENATO O. RESENDE, INSTITUTO DE CIENCIAS BIOLÓGICAS, DEPARTAMENTO DE BIOLOGIA CELULAR, UNIVERSIDADE DE BRASÍLIA.; RICHARD KORMELINK, LABORATORY OF VIROLOGY, DEPARTMENT OF PLANT SCIENCES, WAGENINGEN UNIVERSITY. |
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Título: |
The tomato spotted wilt virus cell-to-cell movement protein (NSM) triggers a hypersensitive response in Sw-5-containing resistant tomato lines and in Nicotiana benthamiana transformed with the functional Sw-5b resistance gene copy. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Molecular Plant Pathology, v. 15, n. 9, p. 871-880, 2014. |
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ISSN: |
1364-3703 |
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DOI: |
10.1111/mpp.12144 |
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Idioma: |
Inglês |
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Conteúdo: |
Although the Sw-5 gene cluster has been cloned, and Sw-5b has been identified as the functional gene copy that confers resistance to Tomato spotted wilt virus (TSWV), its avirulence (Avr) determinant has not been identified to date. Nicotiana tabacum ?SR1? plants transformed with a copy of the Sw-5b gene are immune without producing a clear visual response on challenge with TSWV, whereas it is shown here that N. benthamiana transformed with Sw-5b gives a rapid and conspicuous hypersensitive response (HR). Using these plants, from all structural and non-structural TSWV proteins tested, the TSWV cell-to-cell movement protein (NSM) was confirmed as the Avr determinant using a Potato virus X (PVX) replicon or a non-replicative pEAQ-HT expression vector system. HR was induced in Sw-5b-transgenic N. benthamiana as well as in resistant near-isogenic tomato lines after agroinfiltration with a functional cell-to-cell movement protein (NSM) from a resistance-inducing (RI) TSWV strain (BR-01), but not with NSM from a Sw-5 resistance-breaking (RB) strain (GRAU).This is the first biological demonstration that Sw-5 -mediated resist-ance is triggered by the TSWV NSM cell-to-cell movement protein. |
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Palavras-Chave: |
Avirulence; Cell-to-cell movement; Resistance gene; Sw-5; TSWV. |
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Thesaurus Nal: |
Tospovirus. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02186naa a2200301 a 4500
001 2006718
005 2015-01-26
008 2014 bl uuuu u00u1 u #d
022 $a1364-3703
024 7 $a10.1111/mpp.12144$2DOI
100 1 $aHALLWASS, M.
245 $aThe tomato spotted wilt virus cell-to-cell movement protein (NSM) triggers a hypersensitive response in Sw-5-containing resistant tomato lines and in Nicotiana benthamiana transformed with the functional Sw-5b resistance gene copy.$h[electronic resource]
260 $c2014
520 $aAlthough the Sw-5 gene cluster has been cloned, and Sw-5b has been identified as the functional gene copy that confers resistance to Tomato spotted wilt virus (TSWV), its avirulence (Avr) determinant has not been identified to date. Nicotiana tabacum ?SR1? plants transformed with a copy of the Sw-5b gene are immune without producing a clear visual response on challenge with TSWV, whereas it is shown here that N. benthamiana transformed with Sw-5b gives a rapid and conspicuous hypersensitive response (HR). Using these plants, from all structural and non-structural TSWV proteins tested, the TSWV cell-to-cell movement protein (NSM) was confirmed as the Avr determinant using a Potato virus X (PVX) replicon or a non-replicative pEAQ-HT expression vector system. HR was induced in Sw-5b-transgenic N. benthamiana as well as in resistant near-isogenic tomato lines after agroinfiltration with a functional cell-to-cell movement protein (NSM) from a resistance-inducing (RI) TSWV strain (BR-01), but not with NSM from a Sw-5 resistance-breaking (RB) strain (GRAU).This is the first biological demonstration that Sw-5 -mediated resist-ance is triggered by the TSWV NSM cell-to-cell movement protein.
650 $aTospovirus
653 $aAvirulence
653 $aCell-to-cell movement
653 $aResistance gene
653 $aSw-5
653 $aTSWV
700 1 $aOLIVEIRA, A. S. de
700 1 $aDIANESE, E. de C.
700 1 $aLOHUIS, D.
700 1 $aBOITEUX, L. S.
700 1 $aNAGATA, A. K. I.
700 1 $aRESENDE, R. O.
700 1 $aKORMELINK, R.
773 $tMolecular Plant Pathology$gv. 15, n. 9, p. 871-880, 2014.
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Embrapa Hortaliças (CNPH) |
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Registro Completo
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Biblioteca(s): |
Embrapa Instrumentação; Embrapa Pecuária Sudeste. |
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Data corrente: |
14/12/2017 |
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Data da última atualização: |
14/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PEREIRA, E. I.; NOGUEIRA, A. R. de A.; CRUZ, C. C. T. da; GUIMARAES, G. G. F.; FOSCHINI, M. M.; BERNARDI, A. C. de C.; OLIVEIRA, C. R. de. |
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Afiliação: |
Elaine Inacio Pereira, Bolsista/Embrapa Pecuária Sudeste; ANA RITA DE ARAUJO NOGUEIRA, CPPSE; Camila Conceição Tomé da Cruz, UFSCar; Gelton Geraldo Fernandes Guimarães, Bolsista/Embrapa Instrumentação; Milene Mitsuyuki Foschini, Bolsista/Embrapa Instrumentação; ALBERTO CARLOS DE CAMPOS BERNARDI, CPPSE; CAUE RIBEIRO DE OLIVEIRA, CNPDIA. |
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Título: |
Controlled urea release employing nanocomposites increases the efficiency of nitrogen use by forage. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
ACS Sustainable Chemistry & Engineering, v. 5, n. 11, p. 9993-10001, 2017. |
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ISSN: |
2168-0485 |
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DOI: |
10.1021/acssuschemeng.7b01919 |
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Idioma: |
Inglês |
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Conteúdo: |
The rapid hydrolysis of urea applied to the soil surface causes high rates of NH3 volatilization, leading to adverse environmental impacts and decreased uptake of N by crops. One approach that can be used to improve the efficiency of urea use involves strategies to control its release, such as the coating of granules with polymers. However, the effectiveness of this method can be limited by poor interaction between the coating and the granule surface. We, therefore, propose a novel class of nanocomposite fertilizers, based on clay exfoliation in urea matrices, with or without polymerization using formaldehyde as a strategy to increase the interaction between urea and the additives. A comparative study was performed using various slow-release fertilizers, determining the amounts of volatilized ammonia, dry matter production, and efficiency of urea-N uptake by ryegrass, in a trial carried out in a greenhouse. Interaction, such as solubility, thickness and chemical composition of the composites revealed aspects of the interaction that affected the slow%release behavior of urea in soil and the availability of N for plants. It could be concluded that the controlled release of urea from the nanocomposites decreased NH3 volatilization, resulting in a more constant N availability in the soil and better synchronization with the nutritional demands of the plants. The new fertilizers offer a practical option for increasing urea%N efficiency, reducing environmental impacts caused by NH3 loss, and improving the quality of forage grown on low fertility soils such as Oxisols. MenosThe rapid hydrolysis of urea applied to the soil surface causes high rates of NH3 volatilization, leading to adverse environmental impacts and decreased uptake of N by crops. One approach that can be used to improve the efficiency of urea use involves strategies to control its release, such as the coating of granules with polymers. However, the effectiveness of this method can be limited by poor interaction between the coating and the granule surface. We, therefore, propose a novel class of nanocomposite fertilizers, based on clay exfoliation in urea matrices, with or without polymerization using formaldehyde as a strategy to increase the interaction between urea and the additives. A comparative study was performed using various slow-release fertilizers, determining the amounts of volatilized ammonia, dry matter production, and efficiency of urea-N uptake by ryegrass, in a trial carried out in a greenhouse. Interaction, such as solubility, thickness and chemical composition of the composites revealed aspects of the interaction that affected the slow%release behavior of urea in soil and the availability of N for plants. It could be concluded that the controlled release of urea from the nanocomposites decreased NH3 volatilization, resulting in a more constant N availability in the soil and better synchronization with the nutritional demands of the plants. The new fertilizers offer a practical option for increasing urea%N efficiency, reducing environmental impacts caused by NH3... Mostrar Tudo |
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Palavras-Chave: |
Ammonia loss; Urea controlled release. |
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Thesaurus NAL: |
forage. |
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Categoria do assunto: |
W Química e Física |
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Marc: |
LEADER 02391naa a2200253 a 4500
001 2082569
005 2017-12-14
008 2017 bl uuuu u00u1 u #d
022 $a2168-0485
024 7 $a10.1021/acssuschemeng.7b01919$2DOI
100 1 $aPEREIRA, E. I.
245 $aControlled urea release employing nanocomposites increases the efficiency of nitrogen use by forage.$h[electronic resource]
260 $c2017
520 $aThe rapid hydrolysis of urea applied to the soil surface causes high rates of NH3 volatilization, leading to adverse environmental impacts and decreased uptake of N by crops. One approach that can be used to improve the efficiency of urea use involves strategies to control its release, such as the coating of granules with polymers. However, the effectiveness of this method can be limited by poor interaction between the coating and the granule surface. We, therefore, propose a novel class of nanocomposite fertilizers, based on clay exfoliation in urea matrices, with or without polymerization using formaldehyde as a strategy to increase the interaction between urea and the additives. A comparative study was performed using various slow-release fertilizers, determining the amounts of volatilized ammonia, dry matter production, and efficiency of urea-N uptake by ryegrass, in a trial carried out in a greenhouse. Interaction, such as solubility, thickness and chemical composition of the composites revealed aspects of the interaction that affected the slow%release behavior of urea in soil and the availability of N for plants. It could be concluded that the controlled release of urea from the nanocomposites decreased NH3 volatilization, resulting in a more constant N availability in the soil and better synchronization with the nutritional demands of the plants. The new fertilizers offer a practical option for increasing urea%N efficiency, reducing environmental impacts caused by NH3 loss, and improving the quality of forage grown on low fertility soils such as Oxisols.
650 $aforage
653 $aAmmonia loss
653 $aUrea controlled release
700 1 $aNOGUEIRA, A. R. de A.
700 1 $aCRUZ, C. C. T. da
700 1 $aGUIMARAES, G. G. F.
700 1 $aFOSCHINI, M. M.
700 1 $aBERNARDI, A. C. de C.
700 1 $aOLIVEIRA, C. R. de
773 $tACS Sustainable Chemistry & Engineering$gv. 5, n. 11, p. 9993-10001, 2017.
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Embrapa Pecuária Sudeste (CPPSE) |
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