|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
30/11/2012 |
Data da última atualização: |
05/08/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
GOES, K. C. G. P. de; FISHER, M. L. de C.; CATTELAN, A. J.; NOGUEIRA, M. A.; CARVALHO, C. G. P. de; OLIVEIRA, A. L. M. de. |
Afiliação: |
KELLY CAMPOS GUERRA PINHEIRO DE GOES, UEL; MARIA LUISA DE CASTRO FISHER, UEL; ALEXANDRE JOSE CATTELAN, CNPSO; MARCO ANTONIO NOGUEIRA, CNPSO; CLAUDIO GUILHERME PORTELA CARVALHO, CNPSO; ANDRÉ LUIZ MARTINEZ DE OLIVEIRA, UEL. |
Título: |
Biochemical and molecular characterization of high population density bacteria isolated from sunflower. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Journal of Microbiology and Biotechnology, Seoul, v. 22, n. 4, p. 437-447, Feb. 2012. |
DOI: |
10.4014/jmb.1109.09007 |
Idioma: |
Inglês |
Conteúdo: |
Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods, successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing:42 Bacillus (B. subtilis, B. cereus, B.thuringiensis, B.pumilus,B. megaterium, and Bacillus sp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed a broad diversity among the Bacillus isolates, which clustered Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods, successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing: 42 Bacillus (B. subtilis, B. cereus, B. thuringiensis, B. pumilus, B. megaterium, and Bacillus sp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed a broad diversity among the Bacillus isolates, which clustered Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods, successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing: 42 Bacillus (B. subtilis, B. cereus, B. thuringiensis, B. pumilus, B. megaterium, and Bacillus sp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed a broad diversity among the Bacillus isolates, which clustered Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods,successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing: 42 Bacillus (B. subtilis, B.cereus, B. thuringiensis, B. pumilus, B. megaterium, and Bacillussp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed broad diversity among the Bacillus isolates,which clustered into 2 groups with 75% similarity and 13 subgroups with 85% similarity, suggesting that the genetic distance correlated with the source of isolation. The isolates weralso analyzed for certain growth-promoting activitiesAuxin synthesis was widely distributed among the isolates, with values ranging from 93.34 to 1653.37 µM auxin per µg of protein. The phosphate solubilization index ranged from 1.25 to 3.89, and siderophore index varied from 1.15 to 5.25. From a total of 57 isolates, 3 showed an ability to biologically fix atmospheric nitrogen, and 7 showed antagonism against the pathogen Sclerotinia sclerotiorumThe results of biochemical characterization allowed identification of potential candidates for the developmenof biofertilizers targeted to the sunflower crop. MenosNatural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods, successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing:42 Bacillus (B. subtilis, B. cereus, B.thuringiensis, B.pumilus,B. megaterium, and Bacillus sp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed a broad diversity among the Bacillus isolates, which clustered Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the... Mostrar Tudo |
Thesagro: |
Bactéria. |
Categoria do assunto: |
-- |
Marc: |
LEADER 05995naa a2200205 a 4500 001 1940892 005 2017-08-05 008 2012 bl uuuu u00u1 u #d 024 7 $a10.4014/jmb.1109.09007$2DOI 100 1 $aGOES, K. C. G. P. de 245 $aBiochemical and molecular characterization of high population density bacteria isolated from sunflower.$h[electronic resource] 260 $c2012 520 $aNatural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods, successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing:42 Bacillus (B. subtilis, B. cereus, B.thuringiensis, B.pumilus,B. megaterium, and Bacillus sp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed a broad diversity among the Bacillus isolates, which clustered Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods, successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing: 42 Bacillus (B. subtilis, B. cereus, B. thuringiensis, B. pumilus, B. megaterium, and Bacillus sp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed a broad diversity among the Bacillus isolates, which clustered Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods, successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing: 42 Bacillus (B. subtilis, B. cereus, B. thuringiensis, B. pumilus, B. megaterium, and Bacillus sp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed a broad diversity among the Bacillus isolates, which clustered Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods,successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing: 42 Bacillus (B. subtilis, B.cereus, B. thuringiensis, B. pumilus, B. megaterium, and Bacillussp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed broad diversity among the Bacillus isolates,which clustered into 2 groups with 75% similarity and 13 subgroups with 85% similarity, suggesting that the genetic distance correlated with the source of isolation. The isolates weralso analyzed for certain growth-promoting activitiesAuxin synthesis was widely distributed among the isolates, with values ranging from 93.34 to 1653.37 µM auxin per µg of protein. The phosphate solubilization index ranged from 1.25 to 3.89, and siderophore index varied from 1.15 to 5.25. From a total of 57 isolates, 3 showed an ability to biologically fix atmospheric nitrogen, and 7 showed antagonism against the pathogen Sclerotinia sclerotiorumThe results of biochemical characterization allowed identification of potential candidates for the developmenof biofertilizers targeted to the sunflower crop. 650 $aBactéria 700 1 $aFISHER, M. L. de C. 700 1 $aCATTELAN, A. J. 700 1 $aNOGUEIRA, M. A. 700 1 $aCARVALHO, C. G. P. de 700 1 $aOLIVEIRA, A. L. M. de 773 $tJournal of Microbiology and Biotechnology, Seoul$gv. 22, n. 4, p. 437-447, Feb. 2012.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Soja (CNPSO) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registros recuperados : 26 | |
3. | | SANTOS, M. S.; GUNDI, J. S.; BABUJIA, L. C.; OLIVEIRA, A. L. M. de; HUNGRIA, M. Análise da concentração e da sobrevivência de Azospirillum brasilense estirpes AB-V5 e AB-V6 em diferentes meios de cultura. In: REUNIÃO LATINOAMERICANA DE RIZOBIOLOGIA - RELAR, 27., 2016, Londrina. Fortalecendo as parcerias Sul-Sul: anais. Curitiba: SBCS-NEPAR, 2016. p. 278. Editores: Mariangela Hungria, Douglas Fabiano Gomes, Arnaldo Colozzi Filho.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
| |
5. | | GUNDI, J. S.; BABUJIA, L. C.; NOGUEIRA, M. A.; OLIVEIRA, A. L. M. de; HUNGRIA, M. Desenvolvimento de formulações líquidas de inoculantes contendo Rhizobium tropici CIAT 899 para a cultura do feijoeiro. In: REUNIÃO LATINOAMERICANA DE RIZOBIOLOGIA - RELAR, 27., 2016, Londrina. Fortalecendo as parcerias Sul-Sul: anais. Curitiba: SBCS-NEPAR, 2016. p. 290. Editores: Mariangela Hungria, Douglas Fabiano Gomes, Arnaldo Colozzi Filho.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
| |
9. | | REIS, V. M.; OLIVARES, F. L.; OLIVEIRA, A. L. M. de; REIS JUNIOR, F. B.; BALDANI, J. I.; DOBEREINER, Johanna. Technical approaches to inoculate micropropagated sugar cane plants with Acetobacter diazotrophicus. Plant and Soil, Dordrecht, v. 206, p. 205-211, 1999.Biblioteca(s): Embrapa Agrobiologia. |
| |
12. | | REIS, V. M.; OLIVEIRA, A. L. M. de; OLIVARES, F. L.; BALDANI, J. I.; BODDEY, R. M.; URQUIAGA, S. Inoculants for sugar cane: scientific bases for the adoption of the technology for biofuel production. In: INTERNATIONAL CONGRESS ON NITROGEN FIXATION, 15th., INTERNATIONAL CONFERENCE OF THE AFRICAN ASSOCIATION FOR BIOLOGICAL NITROGEN FIXATION, 12th., 2007, Cape Town, South Africa. BNF Applications for poverty alleviation. abstracts... Cape Town: University of Technology & Sister Institutions in Cape Town, 2007. 1 p.Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
Biblioteca(s): Embrapa Agrobiologia. |
| |
13. | | OLIVEIRA, A. L. M. de; CANUTO, E. de L.; URQUIAGA, S.; REIS, V. M.; BALDANI, J. I. Yield of micropropagated sugarcane varieties in different soil types following inoculation with diazotrophic bacteria. Plant and Soil, Dordrecht, v. 284, n. 1-2, p. 23-32, jun. 2006.Biblioteca(s): Embrapa Agrobiologia. |
| |
14. | | MATSUMOTO, L. S.; SATURNO, D. F.; OLIVEIRA, A. L. M. de; SILVA, A. P.; BABUJA, L. C.; NOGUEIRA, M. A.; HUNGRIA, M. Avaliação da eficiência do inoculante contendo estirpes de Azospirillum brasilense em variedades de cana-de-açúcar. In: CONGRESSO BRASILEIRO DE MICROBILOGIA, 26.; SIMPÓSIO INTERNACIONAL DE BACTÉRIAS LÁTICAS; ENCONTRO NACIONAL DE PROFESSORES DE MICROBIOLOGIA; SIMPÓSIO DE COLEÇÕES DE CULTURAS, 4., 2011, Foz do Iguaçu. Anais... São Paulo: SBM, 2011. Resumo, 916-1.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
| |
15. | | GOES, K. C. G. P. de; FISHER, M. L. de C.; CATTELAN, A. J.; NOGUEIRA, M. A.; CARVALHO, C. G. P. de; OLIVEIRA, A. L. M. de. Biochemical and molecular characterization of high population density bacteria isolated from sunflower. Journal of Microbiology and Biotechnology, Seoul, v. 22, n. 4, p. 437-447, Feb. 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Soja. |
| |
17. | | RODRIGUES, E. P.; RODRIGUES, L. da S.; OLIVEIRA, A. L. M. de; BALDANI, V. L. D.; TEIXEIRA, K. R. dos S.; URQUIAGA, S.; REIS, V. M. Azospirillum amazonense inoculation: effects on growth, yield and N2 fixation of rice (Oryza sativa L.). Plant and Soil, The Netherlands, v. 302, n. 1-2, p. 249-261, jan. 2008. Parceria: UFRRJ.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
Biblioteca(s): Embrapa Agrobiologia. |
| |
18. | | MATSUMURA, E. E.; SECCO, V. A.; MOREIRA, R. S.; SANTOS, O. J. A. P. dos; HUNGRIA, M.; OLIVEIRA, A. L. M. de. Composition and activity of endophytic bacterial communities in field-grown maize plants inoculated with Azospirillum brasilense. Annals of Microbiology, v. 65, n. 4, p. 2187-2200, Dec. 2015.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Soja. |
| |
19. | | CAVALCANTE, A. C. R.; MOITA, A. K. F.; SALES, G. S.; OLIVEIRA, A. L. M. de; ARAUJO FILHO, J. A. de. Fenologia do juazeiro indiano (Zizyphus undulata Reiss.). In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA UVA, 1., 1998; ENCONTRO DE INICIAÇÃO CIENTÍFICA DA UVA, 2., 2000, Sobral. Anais... Sobral: Universidade Estadual Vale do Acaraú, 2001. p. 15.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
20. | | JAMES, E. K.; OLIVARES, F. L.; OLIVEIRA, A. L. M. de; REIS JÚNIOR, F. B. dos; SILVA, L. G. da; REIS, V. M. Further observations on the interaction between sugar can and Gluconacetobacter diazotrophicus under laboratory and greenhouse conditions. Journal of Experimental Botany, London, v. 52, n. 357, p. 747-760, apr. 2001.Biblioteca(s): Embrapa Agrobiologia. |
| |
Registros recuperados : 26 | |
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|