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| 46. |  | MONTEIRO, J. E. B. de A.; OLIVEIRA, A. F. de; NAKAI, A. M. ICT in agrometeorology and climate change. In: MASSRUHÁ, S. M. F. S.; LEITE, M. A. de A.; LUCHIARI JUNIOR, A.; ROMANI, L. A. S. (Ed.). Information and communication technologies and their relations with agriculture. Brasília, DF: Embrapa, 2016. ch. 7, p. 121-138.| Biblioteca(s): Embrapa Agricultura Digital. |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
03/10/2016 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
SILVA, J.; MORAES, C. M. de; SILVA, C. L.; SALES, G. A.; KEID, L. B.; MATOS, P. C. M.; LARA, A. P. S. S.; MORAES, C. C. G. |
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Afiliação: |
JACQUELINE SILVA, PPG-SAAM/UFPA; CARINA M. DE MORAES, PPG-SAAM/UFPA; CLEYZER L. SILVA, PPG-SAAM/UFPA; GUSTAVO A. SALES, PPG-SAAM/UFPA; LARA B. KEID, USP; PAULO C. M. MATOS, PPG-SAAM/UFPA; ANA P. S. S. LARA, UFPEL; CARLA C. G. MORAES, PPG-SAAM/UFPA. |
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Título: |
Brucella abortus detected in cheese from the Amazon region: differentiation of a vaccine strain (B19) from the field strain in the states of Pará, Amapá and Rondônia, Brazil. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 8, p. 705-710, ago. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Brucellosis is an infectious-contagious disease responsible for significant economic losses to the meat and milk supply chain, because it causes reproductive disorders in animals and is a chronic anthropozoonosis. This study was designed to detect the DNA of Brucella spp. in cheese and to differentiate between a vaccine strain (B19) and the field strain. Sixty-six samples of different cheeses which are produced and marketed in three states of the Brazilian Amazon region (Amapá [5 samples], Pará [55 samples] and Rondônia [6 samples]) were evaluated. Thirty-nine of these samples were from cheeses made from cow’s milk, and 27 were from cheeses made from buffalo milk. Four of the 66 samples were from cheeses produced in milk processing plants regulated by the Federal Inspection Service (Serviço de Inspeção Federal); nine of the samples were from cheeses produced in processing plants regulated by the State Inspection Service (Serviço de Inspeção Estadual); five of the samples were from artisanal cheeses; and the remaining 48 samples were from informally produced cheese. DNA was obtained from the samples following a DNA extraction protocol, and PCR was conducted using primers B4 and B5 to detect Brucella spp. Primers eri1 and eri2 were used to differentiate the field strain from the B19 vaccine strain. The results showed that 21.21% (14/66) of the samples were positive for Brucella spp., of which 21.43% (3/14) were positive for the B. abortus field strain, and 7.14% (1/14) were identified as harboring vaccine strain B19. These results demonstrate that it is possible to dentify Brucella spp. in cheese from the Amazon region using the PCR technique and to differentiate the B. abortus field strain from the B19 vaccine strain. MenosBrucellosis is an infectious-contagious disease responsible for significant economic losses to the meat and milk supply chain, because it causes reproductive disorders in animals and is a chronic anthropozoonosis. This study was designed to detect the DNA of Brucella spp. in cheese and to differentiate between a vaccine strain (B19) and the field strain. Sixty-six samples of different cheeses which are produced and marketed in three states of the Brazilian Amazon region (Amapá [5 samples], Pará [55 samples] and Rondônia [6 samples]) were evaluated. Thirty-nine of these samples were from cheeses made from cow’s milk, and 27 were from cheeses made from buffalo milk. Four of the 66 samples were from cheeses produced in milk processing plants regulated by the Federal Inspection Service (Serviço de Inspeção Federal); nine of the samples were from cheeses produced in processing plants regulated by the State Inspection Service (Serviço de Inspeção Estadual); five of the samples were from artisanal cheeses; and the remaining 48 samples were from informally produced cheese. DNA was obtained from the samples following a DNA extraction protocol, and PCR was conducted using primers B4 and B5 to detect Brucella spp. Primers eri1 and eri2 were used to differentiate the field strain from the B19 vaccine strain. The results showed that 21.21% (14/66) of the samples were positive for Brucella spp., of which 21.43% (3/14) were positive for the B. abortus field strain, and 7.14% (1/14) were id... Mostrar Tudo |
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Palavras-Chave: |
PCR; Vaccines (B19); Vacina B19. |
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Thesagro: |
Bovideo; Brucella abortus; Queijo. |
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Thesaurus NAL: |
Brucella melitensis biovar Abortus; Cattle; Cheeses. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/148211/1/Brucella-abortus-detected.pdf
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Marc: |
LEADER 02713naa a2200313 a 4500
001 2053998
005 2017-12-29
008 2016 bl uuuu u00u1 u #d
100 1 $aSILVA, J.
245 $aBrucella abortus detected in cheese from the Amazon region$bdifferentiation of a vaccine strain (B19) from the field strain in the states of Pará, Amapá and Rondônia, Brazil.
260 $c2016
520 $aBrucellosis is an infectious-contagious disease responsible for significant economic losses to the meat and milk supply chain, because it causes reproductive disorders in animals and is a chronic anthropozoonosis. This study was designed to detect the DNA of Brucella spp. in cheese and to differentiate between a vaccine strain (B19) and the field strain. Sixty-six samples of different cheeses which are produced and marketed in three states of the Brazilian Amazon region (Amapá [5 samples], Pará [55 samples] and Rondônia [6 samples]) were evaluated. Thirty-nine of these samples were from cheeses made from cow’s milk, and 27 were from cheeses made from buffalo milk. Four of the 66 samples were from cheeses produced in milk processing plants regulated by the Federal Inspection Service (Serviço de Inspeção Federal); nine of the samples were from cheeses produced in processing plants regulated by the State Inspection Service (Serviço de Inspeção Estadual); five of the samples were from artisanal cheeses; and the remaining 48 samples were from informally produced cheese. DNA was obtained from the samples following a DNA extraction protocol, and PCR was conducted using primers B4 and B5 to detect Brucella spp. Primers eri1 and eri2 were used to differentiate the field strain from the B19 vaccine strain. The results showed that 21.21% (14/66) of the samples were positive for Brucella spp., of which 21.43% (3/14) were positive for the B. abortus field strain, and 7.14% (1/14) were identified as harboring vaccine strain B19. These results demonstrate that it is possible to dentify Brucella spp. in cheese from the Amazon region using the PCR technique and to differentiate the B. abortus field strain from the B19 vaccine strain.
650 $aBrucella melitensis biovar Abortus
650 $aCattle
650 $aCheeses
650 $aBovideo
650 $aBrucella abortus
650 $aQueijo
653 $aPCR
653 $aVaccines (B19)
653 $aVacina B19
700 1 $aMORAES, C. M. de
700 1 $aSILVA, C. L.
700 1 $aSALES, G. A.
700 1 $aKEID, L. B.
700 1 $aMATOS, P. C. M.
700 1 $aLARA, A. P. S. S.
700 1 $aMORAES, C. C. G.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 8, p. 705-710, ago. 2016.
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