|
|
Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
08/11/2021 |
Data da última atualização: |
08/11/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MARCANO DE HIGUERA, J.; SANTOS, H. M.; OLIVEIRA, A. F. DE; NOGUEIRA, A. R. de A. |
Afiliação: |
JULYMAR MARCANO DE HIGUERA, UFSCAR; HERICK M. SANTOS, UFSCAR; ALINE F. DE OLIVEIRA, Institute of Analytical Chemistry of the Czech Academy of Sciences; ANA RITA DE ARAUJO NOGUEIRA, CPPSE. |
Título: |
Bioaccessibility assessment of Cu, Fe, K, Mg, P, and Zn in thermally treated lamb meat. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Journal of the Brazilian Chemical Society, v.32, n.11, p.2111-2119, 2021. |
DOI: |
https://dx.doi.org/10.21577/0103-5053.20210103 |
Idioma: |
Inglês |
Conteúdo: |
A bioaccessibility test with raw and cooked lamb meat samples was performed. The evaluated cooking devices were grill, microwave oven, air fryer, pressure cooker, and electric oven. Physicochemical parameters and the total mass fraction of Cu, Fe, K, Mg, P, and Zn were determined in raw and cooked samples by inductively coupled plasma optical emission spectrometry (ICP OES). The trueness was evaluated using certified reference materials, with recoveries from 87 to 101%. The pressure cooking presented the major changes, including the highest values of internal temperature, loss of inorganic elements after cooking, and the lowest values of moisture and analyte mass fractions. An in vitro gastrointestinal simulation was performed, and the method was validated by an addition and recovery test, in which the trueness varied from 87 to 115%. The bioaccessibility ranged between 28-56, 4-19, 68-76, 41-54, 48-57, and 1-21% for Cu, Fe, K, Mg, P, and Zn, respectively. The cooking methods promoted changes in the meat samples, thus affecting the bioaccessibility of the nutrients. Based on the recommended dietary intake (RDI) calculation, lamb meat can be considered a good Fe, P, and Zn source. |
Palavras-Chave: |
Bioaccessibility; ICP OES; RDA; Sample preparation. |
Thesaurus Nal: |
Lamb meat. |
Categoria do assunto: |
W Química e Física |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227486/1/BioaccessibilityAssessmentCu.pdf
|
Marc: |
LEADER 01923naa a2200229 a 4500 001 2135878 005 2021-11-08 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://dx.doi.org/10.21577/0103-5053.20210103$2DOI 100 1 $aMARCANO DE HIGUERA, J. 245 $aBioaccessibility assessment of Cu, Fe, K, Mg, P, and Zn in thermally treated lamb meat.$h[electronic resource] 260 $c2021 520 $aA bioaccessibility test with raw and cooked lamb meat samples was performed. The evaluated cooking devices were grill, microwave oven, air fryer, pressure cooker, and electric oven. Physicochemical parameters and the total mass fraction of Cu, Fe, K, Mg, P, and Zn were determined in raw and cooked samples by inductively coupled plasma optical emission spectrometry (ICP OES). The trueness was evaluated using certified reference materials, with recoveries from 87 to 101%. The pressure cooking presented the major changes, including the highest values of internal temperature, loss of inorganic elements after cooking, and the lowest values of moisture and analyte mass fractions. An in vitro gastrointestinal simulation was performed, and the method was validated by an addition and recovery test, in which the trueness varied from 87 to 115%. The bioaccessibility ranged between 28-56, 4-19, 68-76, 41-54, 48-57, and 1-21% for Cu, Fe, K, Mg, P, and Zn, respectively. The cooking methods promoted changes in the meat samples, thus affecting the bioaccessibility of the nutrients. Based on the recommended dietary intake (RDI) calculation, lamb meat can be considered a good Fe, P, and Zn source. 650 $aLamb meat 653 $aBioaccessibility 653 $aICP OES 653 $aRDA 653 $aSample preparation 700 1 $aSANTOS, H. M. 700 1 $aOLIVEIRA, A. F. DE 700 1 $aNOGUEIRA, A. R. de A. 773 $tJournal of the Brazilian Chemical Society$gv.32, n.11, p.2111-2119, 2021.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registro Completo
Biblioteca(s): |
Embrapa Agroenergia; Embrapa Meio-Norte. |
Data corrente: |
26/01/2022 |
Data da última atualização: |
21/02/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
SILVA, T. L. C. da; SILVA, V. N. B.; BRAGA, I. de O.; RODRIGUES NETO, J. C.; LEAO, A. P.; RIBEIRO, J. A. de A.; VALADARES, L. F.; ABDELNUR, P. V.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. |
Afiliação: |
THALLITON LUIZ CARVALHO DA SILVA, Programa de Pós-Graduação em Biotecnologia Vegetal, Universidade Federal de Lavras, MG, Brasil.; VIVIANNY NAYSE BELO SILVA, Programa de Pós-Graduação em Biotecnologia Vegetal, Universidade Federal de Lavras, MG, Brasil.; ÍTALO DE OLIVEIRA BRAGA, Programa de Pós-Graduação em Biotecnologia Vegetal, Universidade Federal de Lavras, MG, Brasil.; JORGE CANDIDO RODRIGUES NETO, Instituto de Química, Universidade Federal de Goiás, Goiânia, GO, Brasil.; ANDRE PEREIRA LEAO, CNPAE; JOSE ANTONIO DE AQUINO RIBEIRO, CNPAE; LEONARDO FONSECA VALADARES, CNPAE; PATRICIA VERARDI ABDELNUR, CNPAE; CARLOS ANTONIO FERREIRA DE SOUSA, CPAMN; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE. |
Título: |
Integration of metabolomics and transcriptomics data to further characterize Gliricidia sepium (Jacq.) Kunth under high salinity stress. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Plant Genome, e20182, 2021. |
Idioma: |
Inglês |
Conteúdo: |
Soil salinity is one abiotic stress that threatens agriculture in more than 100 countries. Gliricidia [Gliricidia sepium (Jacq.) Kunth] is a multipurpose tree known for its ability to adapt to a wide range of soils; however, its tolerance limits and responses to salt stress are not yet well understood. In this study, after characterizing the morphophysiological responses of young gliricidia plants to salinity stress, leaf metabolic and transcription profiles were generated and submitted to single and integrated analyses. RNA from leaf samples were subjected to RNA sequencing using an Illumina HiSeq platform and the paired-end strategy. Polar and lipidic fractions from leaf samples were extracted and analyzed on an ultra-high-performance liquid chromatography (UHPLC) coupled with electrospray ionization quadrupole time-offlight high-resolution mass spectrometry (MS) system. Acquired data were analyzed using the OmicsBox, XCMS Online, MetaboAnalyst, and Omics Fusion platforms. The substrate salinization protocol used allowed the identification of two distinct responses to salt stress: tolerance and adaptation. Single analysis on transcriptome and metabolome data sets led to a group of 5,672 transcripts and 107 metabolites differentially expressed in gliricidia leaves under salt stress. The phenylpropanoid biosynthesis was the most affected pathway, with 15 metabolites and three genes differentially expressed. Results showed that the differentially expressed metabolites and genes from this pathway affect mainly short-term salt stress (STS). The single analysis of the transcriptome identified 12 genes coding for proteins that might play a role in gliricidia response at both STS and long-termsalt stress (LTS). Further studies are needed to reveal the mechanisms behind the adaptation response. MenosSoil salinity is one abiotic stress that threatens agriculture in more than 100 countries. Gliricidia [Gliricidia sepium (Jacq.) Kunth] is a multipurpose tree known for its ability to adapt to a wide range of soils; however, its tolerance limits and responses to salt stress are not yet well understood. In this study, after characterizing the morphophysiological responses of young gliricidia plants to salinity stress, leaf metabolic and transcription profiles were generated and submitted to single and integrated analyses. RNA from leaf samples were subjected to RNA sequencing using an Illumina HiSeq platform and the paired-end strategy. Polar and lipidic fractions from leaf samples were extracted and analyzed on an ultra-high-performance liquid chromatography (UHPLC) coupled with electrospray ionization quadrupole time-offlight high-resolution mass spectrometry (MS) system. Acquired data were analyzed using the OmicsBox, XCMS Online, MetaboAnalyst, and Omics Fusion platforms. The substrate salinization protocol used allowed the identification of two distinct responses to salt stress: tolerance and adaptation. Single analysis on transcriptome and metabolome data sets led to a group of 5,672 transcripts and 107 metabolites differentially expressed in gliricidia leaves under salt stress. The phenylpropanoid biosynthesis was the most affected pathway, with 15 metabolites and three genes differentially expressed. Results showed that the differentially expressed metabolites and gen... Mostrar Tudo |
Palavras-Chave: |
Adaptation; Salinization protocol. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/230514/1/The-Plant-Genome-2022-Integration.pdf
|
Marc: |
LEADER 02601naa a2200253 a 4500 001 2139329 005 2022-02-21 008 2021 bl uuuu u00u1 u #d 100 1 $aSILVA, T. L. C. da 245 $aIntegration of metabolomics and transcriptomics data to further characterize Gliricidia sepium (Jacq.) Kunth under high salinity stress.$h[electronic resource] 260 $c2021 520 $aSoil salinity is one abiotic stress that threatens agriculture in more than 100 countries. Gliricidia [Gliricidia sepium (Jacq.) Kunth] is a multipurpose tree known for its ability to adapt to a wide range of soils; however, its tolerance limits and responses to salt stress are not yet well understood. In this study, after characterizing the morphophysiological responses of young gliricidia plants to salinity stress, leaf metabolic and transcription profiles were generated and submitted to single and integrated analyses. RNA from leaf samples were subjected to RNA sequencing using an Illumina HiSeq platform and the paired-end strategy. Polar and lipidic fractions from leaf samples were extracted and analyzed on an ultra-high-performance liquid chromatography (UHPLC) coupled with electrospray ionization quadrupole time-offlight high-resolution mass spectrometry (MS) system. Acquired data were analyzed using the OmicsBox, XCMS Online, MetaboAnalyst, and Omics Fusion platforms. The substrate salinization protocol used allowed the identification of two distinct responses to salt stress: tolerance and adaptation. Single analysis on transcriptome and metabolome data sets led to a group of 5,672 transcripts and 107 metabolites differentially expressed in gliricidia leaves under salt stress. The phenylpropanoid biosynthesis was the most affected pathway, with 15 metabolites and three genes differentially expressed. Results showed that the differentially expressed metabolites and genes from this pathway affect mainly short-term salt stress (STS). The single analysis of the transcriptome identified 12 genes coding for proteins that might play a role in gliricidia response at both STS and long-termsalt stress (LTS). Further studies are needed to reveal the mechanisms behind the adaptation response. 653 $aAdaptation 653 $aSalinization protocol 700 1 $aSILVA, V. N. B. 700 1 $aBRAGA, I. de O. 700 1 $aRODRIGUES NETO, J. C. 700 1 $aLEAO, A. P. 700 1 $aRIBEIRO, J. A. de A. 700 1 $aVALADARES, L. F. 700 1 $aABDELNUR, P. V. 700 1 $aSOUSA, C. A. F. de 700 1 $aSOUZA JUNIOR, M. T. 773 $tPlant Genome, e20182, 2021.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Agroenergia (CNPAE) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|