Data/hora: 05/06/2024 09:53:31

Biblioteca(s): | Embrapa Caprinos e Ovinos; Embrapa Gado de Leite.
Data corrente: | 25/05/2015
Data da última atualização: | 05/02/2018
Autoria: | OLIVARES, C. C. S.; FONSECA, J. F. da; CAMARGO, L. S. de A.; FABJAN, J. M. G. de S.; RODRIGUES, A. L. R.; BRANDÃO, F. Z.
Afiliação: | Carolina Cerqueira Sarmento Olivares, Faculty of Veterinary Medicine, Fluminense Federal University - Niterói, RJ, Brazil; JEFERSON FERREIRA DA FONSECA, CNPC; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Joanna Maria Gonçalves de Souza Fabjan, Faculty of Veterinary Medicine, Fluminense Federal University - Niterói, RJ, Brazil; André Luís Rios Rodrigues, Faculty of Veterinary Medicine, Fluminense Federal University - Niterói, RJ, Brazil; Felipe Zandonadi Brandão, Faculty of Veterinary Medicine, Fluminense Federal University - Niterói, RJ, Brazil.
Título: | Comparison of different methods of goat sperm selection and capacitation for optimization of assisted reproductive technologies.
Ano de publicação: | 2015
Fonte/Imprenta: | Small Ruminant Research, Amsterdam, v. 127, p. 44-49, June, 2015.
DOI: | doi:10.1016/j.smallrumres.2015.04.009

Idioma:

Inglês
Português

Conteúdo: | Abstract: tThis study evaluated different methods of frozen-thawed sperm selection and capacita-tion in goats for further use in reproductive biotechnologies. In Experiment 1, semen wasprocessed by the following techniques: mini-Percoll, swim-up, or washing by centrifuga-tion. In Experiment 2, mini-Percoll selected-sperm was subjected to capacitation inductionby incubation with: 50 ug/mL heparin, 10 uM, 50 uM, or 100 uM of sodium nitroprusside(SNP). Motility, vigor, acrosome, and plasma membrane (PM) integrity were evaluated afterthawing and after each treatment of sperm selection or capacitation. In Experiment 1, wash-ing by centrifugation presented greater (43%; P < 0.05) spermatozoa recovery rate than theother treatments. The swim-up technique showed the lowest (P < 0.05) progressive motil-ity (41%). Spermatozoa presenting both intact PM (P = 0.0002) and acrosome (P = 0.0004)showed an interaction effect between the buck and swim-up technique. In Experiment2, the addition of 100 M SNP resulted in greater (P < 0.05) motility and vigor (38%; 4.7),respectively, than did heparin (28%; 4.3). An enhancement (P < 0.05) in vigor was obtainedafter all treatments in comparison with the evaluation after thawing (3.3). In conclusion,mini-Percoll was better than swim-up for preparing frozen?thawed goat sperm, whereaswashing by centrifugation technique presented similar rates to mini-Percoll and could alsobe used. The use of 100 M SNP resulted in better motility and vigor than heparin treatment
Palavras-Chave: | Capacitation; Sodium nitroprusside; Sperm preparation; Sperm selection.
Thesagro: | Caprino; Espermatozóide; Reprodução animal; Seleção; Sêmen.
Thesaurus Nal: | goats; reproduction; spermatozoa.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Caprinos e Ovinos (CNPC)

Exemplares:

CNPC - (28726-1) - UPC - AP - DD -  -  -  - DIS
CNPGL - (22298-1) - UPC - AP - DD -  -  -  - DIS