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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
28/10/1999 |
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Data da última atualização: |
28/10/1999 |
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Autoria: |
NAGAOKA, T.; OGIHARA, Y. |
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Afiliação: |
University, Maioka-cho 641-12, Yokohama 244, Japan. |
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Título: |
Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Theor Appl Genet, v.94, p. 597-602, 1997 |
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Idioma: |
Inglês |
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Conteúdo: |
Inter-simple sequence repeat polymorphic DNA (ISSR) was evaluated for its applicability as a genetic marker system in wheat. PCR was carried out with primers that annealed to simple sequence repeats. The resultant products were subjected to agarose-gel electrophoresis, and the banding patterns were compared among six wheat accessions containing diploid, tetraploid, and hexaploid members. Out of 100 examined, 33 primers produced distinguishable as well as polymorphic bands in each of the six accessions. Although most of the primers that gave distinct bands (30 primers out of 33) contained dinucleotide repeats, each of the primers with tri-, tetra-, and penta-nucleotide motifs also yielded discrete bands. |
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Palavras-Chave: |
Markers; Microsatellite dNA; PCR; RAPD; Wheats. |
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Categoria do assunto: |
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Marc: |
LEADER 01255naa a2200193 a 4500
001 1648360
005 1999-10-28
008 1997 bl --- 0-- u #d
100 1 $aNAGAOKA, T.
245 $aApplicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers
260 $c1997
520 $aInter-simple sequence repeat polymorphic DNA (ISSR) was evaluated for its applicability as a genetic marker system in wheat. PCR was carried out with primers that annealed to simple sequence repeats. The resultant products were subjected to agarose-gel electrophoresis, and the banding patterns were compared among six wheat accessions containing diploid, tetraploid, and hexaploid members. Out of 100 examined, 33 primers produced distinguishable as well as polymorphic bands in each of the six accessions. Although most of the primers that gave distinct bands (30 primers out of 33) contained dinucleotide repeats, each of the primers with tri-, tetra-, and penta-nucleotide motifs also yielded discrete bands.
653 $aMarkers
653 $aMicrosatellite dNA
653 $aPCR
653 $aRAPD
653 $aWheats
700 1 $aOGIHARA, Y.
773 $tTheor Appl Genet$gv.94, p. 597-602, 1997
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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