Data/hora: 10/06/2024 14:54:57

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 28/10/1999
Data da última atualização: | 28/10/1999
Autoria: | NAGAOKA, T.; OGIHARA, Y.
Afiliação: | University, Maioka-cho 641-12, Yokohama 244, Japan.
Título: | Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers
Ano de publicação: | 1997
Fonte/Imprenta: | Theor Appl Genet, v.94, p. 597-602, 1997
Idioma: | Inglês
Conteúdo: | Inter-simple sequence repeat polymorphic DNA (ISSR) was evaluated for its applicability as a genetic marker system in wheat. PCR was carried out with primers that annealed to simple sequence repeats. The resultant products were subjected to agarose-gel electrophoresis, and the banding patterns were compared among six wheat accessions containing diploid, tetraploid, and hexaploid members. Out of 100 examined, 33 primers produced distinguishable as well as polymorphic bands in each of the six accessions. Although most of the primers that gave distinct bands (30 primers out of 33) contained dinucleotide repeats, each of the primers with tri-, tetra-, and penta-nucleotide motifs also yielded discrete bands.
Palavras-Chave: | Markers; Microsatellite dNA; PCR; RAPD; Wheats.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (17392-1) - ADD - SP - -- - S11571 -  - S11571 - DIS