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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
06/10/2008 |
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Data da última atualização: |
19/02/2009 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
KIMURA, M.; PEREIRA, M.; FUKUDA, W.; NASCIMENTO, P.; FERNANDES, N.; NUTTI. M.; CARVALHO, J. L. |
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Afiliação: |
Mieko Kimura, UNESP; Marcio Eduardo Canto Pereira, CNPMF; Wania Maria Gonçalves Fukuda, CNPMF; Priscila Nascimento, UNESP; Naiara Fernandes, UNESP; Marilia Nutti, CTAA; José Luiz Carvalho, CTAA. |
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Título: |
Stability of B-carotene during freezer storage of cassava analytical sample. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: SCIENTIFIC MEETING OF THE GLOBAL CASSAVA PARTNERSHIP, 1., 2008, Ghent. Cassava: meeting the challenges of the new millennium. Ghent:: IPBO, 2008. p. 36. |
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Idioma: |
Inglês |
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Notas: |
SP01-12. |
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Conteúdo: |
To establish a strategy that would simultaneously attend the breeder's necessity of harvesting a large number of plants and the analytical capacity of the labs, the B-carotene stability during freezer storage of cassava samples was evaluated. Roots of two varieties of cassava growing in an experimental field at EMBRAPA were harvested, peeled and homogenized in a food processor. analytical sample (3-5g) were directly weighed in extraction tubes and frozen. Simultaneoosly, carotenoids of 200g of the same homogenized cassava was extracted with acetone, partitioned to petroleum ether (0,1% BHT) and collected in a volumetric flask. Aliquots were transferred to tubes, dried under nitrogen and kept at -18°C. One half of the samples were kept at Embrapa's laboratory and total carotenoid content was determined spectrophotometrically every three days. The other half was transported by airplane to UNESP's laboratory and total carotenoid and B-carotene contents were determined by spectrophotometry and HPLC, respectively. Comparison of the results from the two labs showed that the transportation time without refrigeration (12 hrs) did not affect the total carotenoid content of both samples and they were stable during 26 days. Thus, a second experiment was carried out in UNESP's lab using coaxed roots of the same varieties sent by Embrapa. Both, analytical samples and dried extracts, prepared by the same way as in the first experiment and stored at -18°C, were stable over 180 days. These results showed that the analytical samples and dried carotenoid extract can be freezer stored for later analysis. Not having HPLC, the lab can send the dried carotenoid extract to another for B-carotene determination. MenosTo establish a strategy that would simultaneously attend the breeder's necessity of harvesting a large number of plants and the analytical capacity of the labs, the B-carotene stability during freezer storage of cassava samples was evaluated. Roots of two varieties of cassava growing in an experimental field at EMBRAPA were harvested, peeled and homogenized in a food processor. analytical sample (3-5g) were directly weighed in extraction tubes and frozen. Simultaneoosly, carotenoids of 200g of the same homogenized cassava was extracted with acetone, partitioned to petroleum ether (0,1% BHT) and collected in a volumetric flask. Aliquots were transferred to tubes, dried under nitrogen and kept at -18°C. One half of the samples were kept at Embrapa's laboratory and total carotenoid content was determined spectrophotometrically every three days. The other half was transported by airplane to UNESP's laboratory and total carotenoid and B-carotene contents were determined by spectrophotometry and HPLC, respectively. Comparison of the results from the two labs showed that the transportation time without refrigeration (12 hrs) did not affect the total carotenoid content of both samples and they were stable during 26 days. Thus, a second experiment was carried out in UNESP's lab using coaxed roots of the same varieties sent by Embrapa. Both, analytical samples and dried extracts, prepared by the same way as in the first experiment and stored at -18°C, were stable over 180 days. These... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02371naa a2200205 a 4500
001 1637314
005 2009-02-19
008 2008 bl uuuu u00u1 u #d
100 1 $aKIMURA, M.
245 $aStability of B-carotene during freezer storage of cassava analytical sample.
260 $c2008
500 $aSP01-12.
520 $aTo establish a strategy that would simultaneously attend the breeder's necessity of harvesting a large number of plants and the analytical capacity of the labs, the B-carotene stability during freezer storage of cassava samples was evaluated. Roots of two varieties of cassava growing in an experimental field at EMBRAPA were harvested, peeled and homogenized in a food processor. analytical sample (3-5g) were directly weighed in extraction tubes and frozen. Simultaneoosly, carotenoids of 200g of the same homogenized cassava was extracted with acetone, partitioned to petroleum ether (0,1% BHT) and collected in a volumetric flask. Aliquots were transferred to tubes, dried under nitrogen and kept at -18°C. One half of the samples were kept at Embrapa's laboratory and total carotenoid content was determined spectrophotometrically every three days. The other half was transported by airplane to UNESP's laboratory and total carotenoid and B-carotene contents were determined by spectrophotometry and HPLC, respectively. Comparison of the results from the two labs showed that the transportation time without refrigeration (12 hrs) did not affect the total carotenoid content of both samples and they were stable during 26 days. Thus, a second experiment was carried out in UNESP's lab using coaxed roots of the same varieties sent by Embrapa. Both, analytical samples and dried extracts, prepared by the same way as in the first experiment and stored at -18°C, were stable over 180 days. These results showed that the analytical samples and dried carotenoid extract can be freezer stored for later analysis. Not having HPLC, the lab can send the dried carotenoid extract to another for B-carotene determination.
700 1 $aPEREIRA, M.
700 1 $aFUKUDA, W.
700 1 $aNASCIMENTO, P.
700 1 $aFERNANDES, N.
700 1 $aNUTTI. M.
700 1 $aCARVALHO, J. L.
773 $tIn: SCIENTIFIC MEETING OF THE GLOBAL CASSAVA PARTNERSHIP, 1., 2008, Ghent. Cassava: meeting the challenges of the new millennium. Ghent:: IPBO, 2008. p. 36.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| 7. | | BARNI, P. E.; BARBOSA, R. I.; XAUD, H. A. M.; XAUD, M. R.; FEARNSIDE, P. M. Precipitação no extremo norte da Amazônia: distribuição espacial no estado de Roraima, Brasil. Sociedade & Natureza, v. 32, p. 439-456, 15 jul. 2020. Título em inglês: Precipitation in northern Amazonia: Spatial distribution in Roraima, Brazil.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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| 17. | | CARVALHO, L. C. S.; NASCIMENTO, M. T.; BARBOSA, R. I.; CASTILHO, C. V. de. Seed predation and germination of Peltogyne gracilipes (Ducke) in three forest types on Maracá Island, Roraima, Brazil In: ANNUAL MEETING OF THE ASSOCIATION FOR TROPICAL BIOLOGY AND CONSERVATION, 49., 2012, Bonito. Ecology, evolution and sustainable use of tropical biodiversity. [S.l.]: ATBC, 2012.| Tipo: Resumo em Anais de Congresso |
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| 19. | | AGUIAR JUNIOR, A. de; BARBOSA, R. I.; BARBOSA, J. B.; OLIVEIRA JUNIOR, M. C. M. de; PINTO, F. Dispersão, distribuição espacial e potencial invasor da Acacia mangium Willd. na savana (lavrado) de Roraima. In: REUNIÃO REGIONAL DA SBPC, 2010, Boa Vista, RR. Resumos... Boa Vista, RR: UFRR, 2010.| Tipo: Resumo em Anais de Congresso |
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| Registros recuperados : 51 | |
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