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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
06/10/2008 |
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Data da última atualização: |
19/02/2009 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
KIMURA, M.; PEREIRA, M.; FUKUDA, W.; NASCIMENTO, P.; FERNANDES, N.; NUTTI. M.; CARVALHO, J. L. |
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Afiliação: |
Mieko Kimura, UNESP; Marcio Eduardo Canto Pereira, CNPMF; Wania Maria Gonçalves Fukuda, CNPMF; Priscila Nascimento, UNESP; Naiara Fernandes, UNESP; Marilia Nutti, CTAA; José Luiz Carvalho, CTAA. |
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Título: |
Stability of B-carotene during freezer storage of cassava analytical sample. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: SCIENTIFIC MEETING OF THE GLOBAL CASSAVA PARTNERSHIP, 1., 2008, Ghent. Cassava: meeting the challenges of the new millennium. Ghent:: IPBO, 2008. p. 36. |
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Idioma: |
Inglês |
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Notas: |
SP01-12. |
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Conteúdo: |
To establish a strategy that would simultaneously attend the breeder's necessity of harvesting a large number of plants and the analytical capacity of the labs, the B-carotene stability during freezer storage of cassava samples was evaluated. Roots of two varieties of cassava growing in an experimental field at EMBRAPA were harvested, peeled and homogenized in a food processor. analytical sample (3-5g) were directly weighed in extraction tubes and frozen. Simultaneoosly, carotenoids of 200g of the same homogenized cassava was extracted with acetone, partitioned to petroleum ether (0,1% BHT) and collected in a volumetric flask. Aliquots were transferred to tubes, dried under nitrogen and kept at -18°C. One half of the samples were kept at Embrapa's laboratory and total carotenoid content was determined spectrophotometrically every three days. The other half was transported by airplane to UNESP's laboratory and total carotenoid and B-carotene contents were determined by spectrophotometry and HPLC, respectively. Comparison of the results from the two labs showed that the transportation time without refrigeration (12 hrs) did not affect the total carotenoid content of both samples and they were stable during 26 days. Thus, a second experiment was carried out in UNESP's lab using coaxed roots of the same varieties sent by Embrapa. Both, analytical samples and dried extracts, prepared by the same way as in the first experiment and stored at -18°C, were stable over 180 days. These results showed that the analytical samples and dried carotenoid extract can be freezer stored for later analysis. Not having HPLC, the lab can send the dried carotenoid extract to another for B-carotene determination. MenosTo establish a strategy that would simultaneously attend the breeder's necessity of harvesting a large number of plants and the analytical capacity of the labs, the B-carotene stability during freezer storage of cassava samples was evaluated. Roots of two varieties of cassava growing in an experimental field at EMBRAPA were harvested, peeled and homogenized in a food processor. analytical sample (3-5g) were directly weighed in extraction tubes and frozen. Simultaneoosly, carotenoids of 200g of the same homogenized cassava was extracted with acetone, partitioned to petroleum ether (0,1% BHT) and collected in a volumetric flask. Aliquots were transferred to tubes, dried under nitrogen and kept at -18°C. One half of the samples were kept at Embrapa's laboratory and total carotenoid content was determined spectrophotometrically every three days. The other half was transported by airplane to UNESP's laboratory and total carotenoid and B-carotene contents were determined by spectrophotometry and HPLC, respectively. Comparison of the results from the two labs showed that the transportation time without refrigeration (12 hrs) did not affect the total carotenoid content of both samples and they were stable during 26 days. Thus, a second experiment was carried out in UNESP's lab using coaxed roots of the same varieties sent by Embrapa. Both, analytical samples and dried extracts, prepared by the same way as in the first experiment and stored at -18°C, were stable over 180 days. These... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02371naa a2200205 a 4500
001 1637314
005 2009-02-19
008 2008 bl uuuu u00u1 u #d
100 1 $aKIMURA, M.
245 $aStability of B-carotene during freezer storage of cassava analytical sample.
260 $c2008
500 $aSP01-12.
520 $aTo establish a strategy that would simultaneously attend the breeder's necessity of harvesting a large number of plants and the analytical capacity of the labs, the B-carotene stability during freezer storage of cassava samples was evaluated. Roots of two varieties of cassava growing in an experimental field at EMBRAPA were harvested, peeled and homogenized in a food processor. analytical sample (3-5g) were directly weighed in extraction tubes and frozen. Simultaneoosly, carotenoids of 200g of the same homogenized cassava was extracted with acetone, partitioned to petroleum ether (0,1% BHT) and collected in a volumetric flask. Aliquots were transferred to tubes, dried under nitrogen and kept at -18°C. One half of the samples were kept at Embrapa's laboratory and total carotenoid content was determined spectrophotometrically every three days. The other half was transported by airplane to UNESP's laboratory and total carotenoid and B-carotene contents were determined by spectrophotometry and HPLC, respectively. Comparison of the results from the two labs showed that the transportation time without refrigeration (12 hrs) did not affect the total carotenoid content of both samples and they were stable during 26 days. Thus, a second experiment was carried out in UNESP's lab using coaxed roots of the same varieties sent by Embrapa. Both, analytical samples and dried extracts, prepared by the same way as in the first experiment and stored at -18°C, were stable over 180 days. These results showed that the analytical samples and dried carotenoid extract can be freezer stored for later analysis. Not having HPLC, the lab can send the dried carotenoid extract to another for B-carotene determination.
700 1 $aPEREIRA, M.
700 1 $aFUKUDA, W.
700 1 $aNASCIMENTO, P.
700 1 $aFERNANDES, N.
700 1 $aNUTTI. M.
700 1 $aCARVALHO, J. L.
773 $tIn: SCIENTIFIC MEETING OF THE GLOBAL CASSAVA PARTNERSHIP, 1., 2008, Ghent. Cassava: meeting the challenges of the new millennium. Ghent:: IPBO, 2008. p. 36.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 8 | |
| 1. |  | VILAS BOAS, R. L. P.; DELAFRONTE, B.; LEITE, R. S.; MANDARINO, J. M. G.; QUIRINO, J. R.; CORRÊA-FERREIRA, B. S.; LORINI, I.; OLIVEIRA, M. A. Variação no perfil de ácidos graxos em grãos de soja, com diferentes intensidades de ataque de percevejo, da colheita ao armazenamento, utilizando a cromatografia gasosa. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 8., 2013, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2013. p. 185-188. (Embrapa Soja. Documentos, 339).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 2. | | DELAFRONTE, B.; VILAS BOAS R. L. P.; LEITE, R. S.; MANDARINO, J. M. G.; QUIRINO, J. R.; CORRÊA-FERREIRA, B. S; LORINI, I.; OLIVEIRA, M. A. Variação nos teores de óleo e proteína em grãos de soja, com diferentes intensidades de ataque de percevejo, da colheita ao armazenamento, utilizando a espectroscopia no infravermelho próximo (NIR). In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 8., 2013, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2013. p. 55-59. (Embrapa Soja. Documentos, 339).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 3. | | OLIVEIRA, M. A; LORINI, I.; MANDARINO, J. M. G.; LEITE, R. S.; CORRÊA-FERREIRA, B. S.; QUIRINO, J. R.; VILAS BOAS, R. L. P.; DELAFRONTE, B. Diferentes manejos de percevejo e sua influência nos teores de óleo, proteína e acidez dos grãos de soja na colheita e durante o armazenamento. In: CONGRESSO BRASILEIRO DE SOJA, 7.; MERCOSOJA, 2015, Florianópolis. Tecnologia e mercado global: perspectivas para soja: anais. Londrina: Embrapa Soja, 2015. 4 p. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
|  |
| 4. | | OLIVEIRA, M. A. de; LORINI, I.; MANDARINO, J. M. G.; LEITE, R. S.; CORRÊA-FERREIRA, B. S.; QUIRINO, J. R.; VILAS BOAS, R. L. P.; DELAFRONTE, B. Perfil de ácidos graxos em grãos de soja, com diferentes manejos de percevejo, da colheita ao armazenamento, utilizando a cromatografia gasosa. In: CONFERÊNCIA BRASILEIRA DE PÓS-COLHEITA, 6., 2014, Maringá. Anais... Londrina: ABRAPOS, 2014. p. 557-562.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 5. | | OLIVEIRA, M. A.; LORINI, I.; MANDARINO, J. M. G.; LEITE, R. S.; QUIRINO, J. R.; CORRÊA-FERREIRA, B. S.; VILAS BOAS, R. L. P.; DELAFRONTE, B. Perfil de ácidos graxos em grãos de soja, com diferentes manejos de percevejo, da colheita ao armazenamento, utilizando a cromatografia gasosa. In: AMERICAS: INTERNATIONAL CONFERENCE ON SOYBEAN UTILIZATION, 2013, Bento Gonçalves. Proceedings... Brasília, DF: Embrapa, 2013. 1 CD-ROM. 5 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 6. | | OLIVEIRA, M. A. de; LORINI, I.; MANDARINO, J. M. G.; LEITE, R. S.; CORRÊA-FERREIRA, B. S.; QUIRINO, J. R.; VILAS BOAS, R. L. P.; DELAFRONTE, B. Teores de óleo, proteína e acidez em grãos de soja, com diferentes manejos de percevejo, da colheita ao armazenamento, provenientes das safras 2011/2012 e 2012/2013. In: CONFERÊNCIA BRASILEIRA DE PÓS-COLHEITA, 6., 2014, Maringá. Anais... Londrina: ABRAPOS, 2014. p. 579-585.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 7. | | OLIVEIRA, M. A. de; LORINI, I.; MANDARINO, J. M. G.; LEITE, R. S.; CORRÊA-FERREIRA, B. S.; QUIRINO, J. R.; VILAS BOAS, R. L. P.; DELAFRONTE, B. Teores de óleo e proteína em grãos de soja, com diferentes manejos de percevejo, da colheita ao armazenamento, utilizando a espectroscopia no infravermelho próximo (NIR). In: CONFERÊNCIA BRASILEIRA DE PÓS-COLHEITA, 6., 2014, Maringá. Anais... Londrina: ABRAPOS, 2014. p. 571-577.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 8. | | OLIVEIRA, M. A.; LORINI, I.; MANDARINO, J. M. G.; LEITE, R. S.; QUIRINO, J. R.; BEATRIZ SPALDING CORRÊA-FERREIRA; VILAS BOAS, R. L. P.; DELAFRONTE, B. Teores de óleo e proteína em grãos de soja, com diferentes manejos de percevejo, da colheita ao armazenamento, utilizando a espectroscopia no infravermelho próximo (NIR). In: AMERICAS: INTERNATIONAL CONFERENCE ON SOYBEAN UTILIZATION, 2013, Bento Gonçalves. Proceedings... Brasília, DF: Embrapa, 2013. 1 CD-ROM. 5 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 8 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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