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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
15/07/2009 |
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Data da última atualização: |
26/06/2012 |
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Autoria: |
CORAZZA-NUNESI; MACHADO, M. A.; STACH-MACHADO, D. R.; NUNES, W. M. C.; CARVALHO, S. A. de; MÜLLERV, G. W. |
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Afiliação: |
MARIA JÚLIA CORAZZA-NUNES, Depto. de Biologia, NBA/UEM; MARCOS ANTONIO MACHADO, C.C.S.M.-IAC; DAGMAR RUTH STACH-MACHADO, Depto. de Imunologia/UNICAMP; WILLIAM MÁRIO CARVALHO NUNES, Depto. de Agronomia, NBA/UEM; SÉRGIO ALVES DE CARVALHO, C.C.S.M.-IAC; GERD WALTER MÜLLER, NBA/UEM. |
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Título: |
Characterization of Citrus tristeza virus isolates from grapefruit (Citrus paradisi Macf.) accessions of Citrus Active Germplasm Bank. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Summa Phytopathologica, Botucatu, v. 32, n. 4, p. 322-327, Oct./Dec. 2006. |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus tristeza virus (CTV) isolates from 35 grapefruit accessions belonging to Citrus Active Germplasm Bank of the "Instituto Agronômico de Campinas" located at the "Centro APTA Citros Sylvio Moreira", Cordeirópolis, São Paulo state, Brazil, were characterized and evaluated through symptoms in the trees, biological indexing, immunological diagnosis with different monoclonal antibodies and SSCP analysis (single-strand conformation polymorphism) of the coat protein gene. Symptomatology indicated that, in general, the group of plants with smaller canopy volume and severe stem pitting differed significantly from the group that presented greater vegetative development and mild to moderate stem pitting. However, the isolates from most of the accessions induced mild reaction on Mexican lime. The serological evaluation through the DAS-ELISA using monoclonal antibodies did not reveal any association between virus titer in the plant tissue and symptoms. The reaction with different monoclonal antibodies and the distinct electrophoresis patterns obtained through SSCP showed that there is a high degree of diversity among the isolates that infect these grapefruit accessions. High complexity within the same isolate was also observed in the SSCP profiles. This finding indicates that the CTV isolates from these plants are a complex mixture of CTV haplotypes. Similar SSCP banding patterns were observed among some plants with strong stem pitting symptoms, and among some plants with weak or moderate stem pitting symptoms. MenosCitrus tristeza virus (CTV) isolates from 35 grapefruit accessions belonging to Citrus Active Germplasm Bank of the "Instituto Agronômico de Campinas" located at the "Centro APTA Citros Sylvio Moreira", Cordeirópolis, São Paulo state, Brazil, were characterized and evaluated through symptoms in the trees, biological indexing, immunological diagnosis with different monoclonal antibodies and SSCP analysis (single-strand conformation polymorphism) of the coat protein gene. Symptomatology indicated that, in general, the group of plants with smaller canopy volume and severe stem pitting differed significantly from the group that presented greater vegetative development and mild to moderate stem pitting. However, the isolates from most of the accessions induced mild reaction on Mexican lime. The serological evaluation through the DAS-ELISA using monoclonal antibodies did not reveal any association between virus titer in the plant tissue and symptoms. The reaction with different monoclonal antibodies and the distinct electrophoresis patterns obtained through SSCP showed that there is a high degree of diversity among the isolates that infect these grapefruit accessions. High complexity within the same isolate was also observed in the SSCP profiles. This finding indicates that the CTV isolates from these plants are a complex mixture of CTV haplotypes. Similar SSCP banding patterns were observed among some plants with strong stem pitting symptoms, and among some plants with weak or mo... Mostrar Tudo |
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Palavras-Chave: |
Anticorpos monoclonais; Citrus paradisi Macf; Sintomas de caneluras; SSCP; Vírus da tristeza dos citrus. |
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Thesagro: |
Elisa. |
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Thesaurus Nal: |
Enzyme-linked immunosorbent assay. |
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Categoria do assunto: |
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Marc: |
LEADER 02384naa a2200265 a 4500
001 1083049
005 2012-06-26
008 2006 bl uuuu u00u1 u #d
100 1 $aCORAZZA-NUNESI
245 $aCharacterization of Citrus tristeza virus isolates from grapefruit (Citrus paradisi Macf.) accessions of Citrus Active Germplasm Bank.
260 $c2006
520 $aCitrus tristeza virus (CTV) isolates from 35 grapefruit accessions belonging to Citrus Active Germplasm Bank of the "Instituto Agronômico de Campinas" located at the "Centro APTA Citros Sylvio Moreira", Cordeirópolis, São Paulo state, Brazil, were characterized and evaluated through symptoms in the trees, biological indexing, immunological diagnosis with different monoclonal antibodies and SSCP analysis (single-strand conformation polymorphism) of the coat protein gene. Symptomatology indicated that, in general, the group of plants with smaller canopy volume and severe stem pitting differed significantly from the group that presented greater vegetative development and mild to moderate stem pitting. However, the isolates from most of the accessions induced mild reaction on Mexican lime. The serological evaluation through the DAS-ELISA using monoclonal antibodies did not reveal any association between virus titer in the plant tissue and symptoms. The reaction with different monoclonal antibodies and the distinct electrophoresis patterns obtained through SSCP showed that there is a high degree of diversity among the isolates that infect these grapefruit accessions. High complexity within the same isolate was also observed in the SSCP profiles. This finding indicates that the CTV isolates from these plants are a complex mixture of CTV haplotypes. Similar SSCP banding patterns were observed among some plants with strong stem pitting symptoms, and among some plants with weak or moderate stem pitting symptoms.
650 $aEnzyme-linked immunosorbent assay
650 $aElisa
653 $aAnticorpos monoclonais
653 $aCitrus paradisi Macf
653 $aSintomas de caneluras
653 $aSSCP
653 $aVírus da tristeza dos citrus
700 1 $aMACHADO, M. A.
700 1 $aSTACH-MACHADO, D. R.
700 1 $aNUNES, W. M. C.
700 1 $aCARVALHO, S. A. de
700 1 $aMÜLLERV, G. W.
773 $tSumma Phytopathologica, Botucatu$gv. 32, n. 4, p. 322-327, Oct./Dec. 2006.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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