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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
24/10/2017 |
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Data da última atualização: |
01/02/2021 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
CLEMENTE, Z.; SILVA, G. H. da; MARTINEZ, D. S. T.; MAURER-MORELLI, C. V.; NUNES, M. C. S.; THOMAZ, A. A.; CASTRO, V. L. S. S. de. |
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Afiliação: |
VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA. |
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Título: |
Exploring the mechanisms of graphene oxide toxicity to zebrafish embryo. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Applied Research in Toxicology, v. 2, p. 56, 2017. Suppl. 1. Abstracts of International Symposium on Toxicity Assesment, 18., 2017, Limeira. Ref. ISTA18-705454. |
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Idioma: |
Inglês |
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Conteúdo: |
Many applications of graphene oxide (GO) are still in the research phase, but its promising uses have raised concerns about their risks to human health and the environment. Environmental factors, as the presence of humic substances in aquatic environment, can affect nanomaterials behavior and its toxicological effects. A previous study of our group revealed that the exposure of zebrafish embryos to GO is related to a reduction in total body length and in acetylcholinesterase activity. Thus, the aim of this study was to investigate the implications of GO exposure in muscular development and acetylcholinesterase expression. Zebrafish embryos (n=48/group) were exposed to GO (100 mg/L) in the presence or absence of humic acid (HA, 20 mg/L) during 5 days. Larvae were not feed during the experiment and were exposed under a 14/10 h light/dark cycle, 26 ± 1oC. The mortality in all groups was below 10% and no malformation was observed. At the end of the experiment, alive larvae were fixed with PFA 4% to evaluate its muscular morphology (confocal microscopy, SHG technique) or frozen in liquid nitrogen to perform RT-PCR analysis (AchE expression). Measurements of sarcomere and somite length were performed. No change in muscular tissue was observed, neither differences in AchE expression among groups were detected. So, further experiments have to be performed to clarify the mechanisms of graphene toxicity. FAPESP grants 2014/01995-9, 2014/12891-0, 2014/15640-8. |
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Palavras-Chave: |
Zebrafish. |
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Categoria do assunto: |
W Química e Física |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/165499/1/RA-CastroVLSS-ISTA18-ART-2017-PA....pdf
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Marc: |
LEADER 02169nam a2200193 a 4500
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100 1 $aCLEMENTE, Z.
245 $aExploring the mechanisms of graphene oxide toxicity to zebrafish embryo.$h[electronic resource]
260 $aApplied Research in Toxicology, v. 2, p. 56, 2017. Suppl. 1. Abstracts of International Symposium on Toxicity Assesment, 18., 2017, Limeira. Ref. ISTA18-705454.$c2017
520 $aMany applications of graphene oxide (GO) are still in the research phase, but its promising uses have raised concerns about their risks to human health and the environment. Environmental factors, as the presence of humic substances in aquatic environment, can affect nanomaterials behavior and its toxicological effects. A previous study of our group revealed that the exposure of zebrafish embryos to GO is related to a reduction in total body length and in acetylcholinesterase activity. Thus, the aim of this study was to investigate the implications of GO exposure in muscular development and acetylcholinesterase expression. Zebrafish embryos (n=48/group) were exposed to GO (100 mg/L) in the presence or absence of humic acid (HA, 20 mg/L) during 5 days. Larvae were not feed during the experiment and were exposed under a 14/10 h light/dark cycle, 26 ± 1oC. The mortality in all groups was below 10% and no malformation was observed. At the end of the experiment, alive larvae were fixed with PFA 4% to evaluate its muscular morphology (confocal microscopy, SHG technique) or frozen in liquid nitrogen to perform RT-PCR analysis (AchE expression). Measurements of sarcomere and somite length were performed. No change in muscular tissue was observed, neither differences in AchE expression among groups were detected. So, further experiments have to be performed to clarify the mechanisms of graphene toxicity. FAPESP grants 2014/01995-9, 2014/12891-0, 2014/15640-8.
653 $aZebrafish
700 1 $aSILVA, G. H. da
700 1 $aMARTINEZ, D. S. T.
700 1 $aMAURER-MORELLI, C. V.
700 1 $aNUNES, M. C. S.
700 1 $aTHOMAZ, A. A.
700 1 $aCASTRO, V. L. S. S. de
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Biblioteca |
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Tipo/Formato |
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Registro |
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URL |
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| Registros recuperados : 31 | |
| 22. |  | CLEMENTE, Z.; SILVA, G. H. da; NUNES, M. C. S.; MARTINEZ, D. S. T.; MAURER-MORELLI, C. V.; THOMAZ, A. A.; CASTRO, V. L. S. S. de. Exploring the mechanisms of graphene oxide behavioral and morphological changes in zebrafish. Environmental Science and Pollution Research, v. 26, n. 29, p. 30508-30523, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Meio Ambiente. |
|    |
| 23. | | CLEMENTE, Z.; SILVA, G. H. da; MARTINEZ, D. S. T.; MAURER-MORELLI, C. V.; NUNES, M. C. S.; THOMAZ, A. A.; CASTRO, V. L. S. S. de. Exploring the mechanisms of graphene oxide toxicity to zebrafish embryo. Applied Research in Toxicology, v. 2, p. 56, 2017. Suppl. 1. Abstracts of International Symposium on Toxicity Assesment, 18., 2017, Limeira. Ref. ISTA18-705454.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 24. | | VIANA, D. S.; CARVALHO, L. M. J. de; PEREIRA, E. J.; CARVALHO, J. L. V. de; NUTTI, M. R.; ROCHA, M. M.; NUNES, M. C. Poliphenols content in raw cowpea (Vigna unguiculata L. Walp.). In: WORLD CONGRESS OF FOOD SCIENCE AND TECHNOLOGY, 16.; LATIN AMERICAN SEMINAR OF FOOD SCIENCE AND TECHNOLOGY, 17., 2012, Foz do Iguaçu. Addressing global food security and wellness through food science and technology: abstracts. Foz do Iguaçu: IUFoST: ALACCTA, 2012. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 26. | | CARVALHO, L. M. J. de; GODOY, R. L. de O.; PACHECO, S.; GOMES, P. B.; FONTES, R. R.; NUNES, M. C.; GLOBA, M.; RIBEIRO, E. M. G.; RAMOS, S. R. R.; CARVALHO, J. L. V. de; NUTTI, M. R. Avaliação do conteúdo de carotenoides totais, alfa e beta-caroteno e isômeros cis em abóboras cruas (Cucurbita moschata Duch.). In: REUNIÃO DE BIOFORTIFICAÇÃO NO BRASIL, 4., 2011, Teresina. Palestras e resumos... Rio de Janeiro: Embrapa Agroindústria de Alimentos; Teresina: Embrapa Meio-Norte, 2011. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 27. | | CARVALHO, M. J. de; GODOY, R. L. de O.; PACHECO, S.; ASSUMPCAO, S.; GOMES, P. B.; FONTES, R. R.; NUNES, M. C.; GLOBA, M.; RIBEIRO, E. M. G.; RAMOS, S. R. R.; CARVALHO, J. L. V. de; NUTTI, M. R. Assessment of total carotenoids, a and B-carotene and, cis B-carotene isomers in pumpkin (Cucurbita moschata Duch.) cooked with sugar addition. In: INTERNATIONAL ISEKI FOOD CONFERENSCE, 2., 2011, Milan. Abtracts... Milan: ISEKI Food Association, 2011. Resumo em anais.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 28. | | CARVALHO, L. M. J. DE.; VIANA, D. S.; PEREIRA, E. J.; CONTE, C.; CARVALHO, J. L. V. de; ROCHA, M. M.; NUNES, M. C.; FREITAS, S. C. de; NUTTI, M. R. Phytic acid in different cowpea (Vigna Unguiculata L. Walp.) cultivars. In: INTERNATIONAL CONFERENCE ON FOOD INOVATION , 3., 2014, Entre Riós. Libro resúmenes. Concordia: Universidad Nacional de Entre Ríos, 2014. p. 359. Ref. A957. 20 al 23 de octubre de 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 29. | | CARVALHO, L. M. J. de; GODOY, R. L. de O.; PACHECO, S.; ASSUMPÇÃO, S.; GOMES, P. B.; FONTES, R. R.; NUNES, M. C.; GLOBA, M.; RIBEIRO, E. M. G.; RAMOS, S. S. S.; CARVALHO, J. L. V. de; NUTTI, M. R. Assessment of total carotenoids, a and B-carotene and, cis B-carotene isomers in pumpkin (Cucurbita moschata Duch.) cooked with sugar addition. In: INTERNATIONAL ISEKI_FOOD CONFERENCE, 2., 2011, Milan. Bridging training and research for industry and the wider community: book of abstracts. Vienna: ISEKI-Food Association, 2011. Ref. P178.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 30. | | CARVALHO, L. M. J. de; GODOY, R. L. de O.; PACHECO, S.; ASSUMPCAO, S.; GOMES, P. B.; FONTES, R. R.; NUNES, M. C.; GLOBA, M.; RIBEIRO, E. M. G.; RAMOS, S. R. R.; CARVALHO, J. L. V. de; NUTTI, M. R. Assessment of total catotenoid, a and B-carotene and cis isomers in raw Cucurbita moschata Duch. In: INTERNATIONAL ISEKI FOOD CONFERENSCE, 2., 2011, Milan. Abtracts... Milan: ISEKI Food Association, 2011. Resumo em anais.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 31. | | CARVALHO, L. M. J. de; GODOY, R. L. de O.; PACHECO, S.; ASSUMPÇÃO, S.; GOMES, P. B.; FONTES, R. R.; NUNES, M. C.; GLOBA, M.; RIBEIRO, E. M. G.; RAMOS, S. S. S.; CARVALHO, J. L. V. de; NUTTI, M. R. Assessment of total catotenoid, a and B-carotene and cis isomers in raw Cucurbita moschata Duch. In: INTERNATIONAL ISEKI_FOOD CONFERENCE, 2., 2011, Milan. Bridging training and research for industry and the wider community: book of abstracts. Vienna: ISEKI-Food Association, 2011. Ref. P183.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| Registros recuperados : 31 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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