Data/hora: 07/06/2024 16:55:27

Biblioteca(s): | Embrapa Café.
Data corrente: | 04/01/2023
Data da última atualização: | 04/01/2023
Autoria: | BARBOSA, E. A.; ALVES, G. S. C.; COURA, M. de M. A.; SILVA, H. de L. e; ROCHA, F. S. da; NUNES, J. B.; WATANABE, M. de S.; ANDRADE, A. C.; BRANDA, G. D.
Afiliação: | EDER ALVES BARBOSA, UNIVERSIDADE DE BRASÍLIA; GABRIEL SÉRGIO COSTA ALVES, UNIVERSIDADE DE BRASÍLIA; MARCELO DE MELO ANDRADE COURA, UNIVERSIDADE DE BRASÍLIA; HIGOR DE LIMA E SILVA, UNIVERSIDADE DE BRASÍLIA; FILIPE SOUZA DA ROCHA, UNIVERSIDADE DE BRASÍLIA; JOÃO BUENO NUNES, UNIVERSIDADE DE BRASÍLIA; MATHEUS DE SOUZA WATANABE, UNIVERSIDADE DE BRASÍLIA; ALAN CARVALHO ANDRADE, CNPCa; GUILHERME DOTTO BRANDA, UNIVERSIDADE DE BRASÍLIA.
Título: | A first look at the N- and O-glycosylation landscape in anuran skin secretions.
Ano de publicação: | 2022
Fonte/Imprenta: | Biochimie, v. 197, Jun., p.19-37, 2022.
DOI: | https://doi.org/10.1016/j.biochi.2022.01.008
Idioma: | Inglês
Conteúdo: | Amphibians secrete a complex array of molecules that shape their interactions with coinhabiting microorganisms and macroscopic predators. Glycans are a rapidly evolving and complex class of biomolecules implicated in intrinsic and extrinsic recognition events. Despite the numerous studies aiming at the biochemical characterization of anuran skin secretions, little is known about protein-linked oligosaccharides, their synthesis pathways, and their homing secreted glycoproteins. In the present report, LC-MS/MS was used to investigate the diversity of N- and O-linked oligosaccharides in the skin secretion of two South American frogs, Pithecopus azureus and Boana raniceps. Additionally, the enzymes responsible for glycan synthesis pathways were evaluated based on their skin tissue transcriptome. Our analyses allowed the annotation of various N- and O-glycan structures commonly found in vertebrate proteins. Paucimannosidic glycans were abundant in the skin secretion of both amphibians; however, hybrid and complex N-glycan structures were detected only in B. raniceps. A good correlation between the structures discovered in glycomic analyses and transcripts encoding enzymes necessary for their synthesis was obtained. Some transcripts such as those of MAN1A2, FUT8, and ST6GALNAC were found solely in B. raniceps. Finally, secreted N- and O- linked glycoproteins were predicted from the transcriptomic data, indicating that proteases and protease inhibitors are putative sources of the glycans described herein. Overall, our results show the presence of oligosaccharides in amphibians skin secretions and suggest that their diversity is species-specific, paving the way for novel perspectives involving amphibian evolution and ecology.
Thesaurus Nal: | Mass spectrometry; Oligosaccharides; Transcriptomics.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1150624/1/A-first-look-at-the-N.pdf
Registro original: | Embrapa Café (CNPCa)
Exemplares: | CNPCa - (1653-1) - UPC - AP - DD -  -  -  - DIS