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Registro Completo |
Biblioteca(s): |
Embrapa Agropecuária Oeste. |
Data corrente: |
17/11/2004 |
Data da última atualização: |
12/08/2013 |
Autoria: |
KURIHARA, C. H.; ALVAREZ V. V. H.; NEVES, J. C. L.; NOVAIS, R. F. de; STAUT, L. A.; SILVA, W. M. |
Título: |
Uso de métodos de diagnose do estado nutricional na definição de valores de referência para nutrientes em soja. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
In: REUNIÃO BRASILEIRA DO SOLO E NUTRIÇÃO DE PLANTAS, 26.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 10.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 8.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 5., 2004. Lages. FERTBIO 2004: [avaliação das conquistas: bases para estratégias futuras: anais]. Lages: SBCS; UDESC Lages, Departamento de Solos, 2004. |
Descrição Física: |
1 CD-ROM. |
Idioma: |
Português |
Palavras-Chave: |
DRIS; Sistema integrado de diagnose e recomendação do nutriente. |
Thesagro: |
Soja. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00976naa a2200217 a 4500 001 1249338 005 2013-08-12 008 2004 bl uuuu u00u1 u #d 100 1 $aKURIHARA, C. H. 245 $aUso de métodos de diagnose do estado nutricional na definição de valores de referência para nutrientes em soja. 260 $c2004 300 $c1 CD-ROM. 650 $aSoja 653 $aDRIS 653 $aSistema integrado de diagnose e recomendação do nutriente 700 1 $aALVAREZ V. V. H. 700 1 $aNEVES, J. C. L. 700 1 $aNOVAIS, R. F. de 700 1 $aSTAUT, L. A. 700 1 $aSILVA, W. M. 773 $tIn: REUNIÃO BRASILEIRA DO SOLO E NUTRIÇÃO DE PLANTAS, 26.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 10.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 8.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 5., 2004. Lages. FERTBIO 2004: [avaliação das conquistas: bases para estratégias futuras: anais]. Lages: SBCS; UDESC Lages, Departamento de Solos, 2004.
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Registro original: |
Embrapa Agropecuária Oeste (CPAO) |
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Registro Completo
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
09/11/2004 |
Data da última atualização: |
23/11/2015 |
Autoria: |
CARVALHO, C. H. S.; ZEHR, U. B.; GUNARATNA, N.; ANDERSON, J.; KONONOWICZ, H. H.; HODGES, T. K.; AXTELL, J. D. |
Afiliação: |
CARLOS HENRIQUE S DE CARVALHO, Embrapa Milho e Sorgo; Usha B. Zehr, Purdue University; Nilupa Gunaratna, Purdue University; Joseph Anderson, Purdue University; Halina H. Kononowicz, Purdue University; Thomas K. Hodges, Purdue University; John D. Axtell, Purdue University. |
Título: |
Agrobacterium-mediated transformation of sorghum: factors that affect transformation efficiency. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 27, n. 2, p. 259-269, 2004. |
Idioma: |
Inglês |
Conteúdo: |
The results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving transformation efficiency. The addition of coconut water to the co-cultivation medium, the use of vigorous and actively growing immature embryos and the removal of excess bacteria significantly improved the survival rate of sorghum embryos and was critical for successful transformation. Hygromycin phosphotransferase (hpt) proved to be a good selectable marker for sorghum. We also found that β-glucuronidase (GUS) activity was low in most of the transgenic plant tissues tested, although it was very high in immature inflorescences. Although promising, the overall transformation efficiency of the protocol is still low and further optimization will require particular attention to be given to the number of Agrobacterium in the inoculum and the selection of sorghum genotypes and explants less sensitive to Agrobacterium infection. MenosThe results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving transformation efficiency. The addition of coconut water to the co-cultivation medium, the use of vigorous and actively growing immature embryos and the removal of excess bacteria significantly improved the survival rate of sorghum embryos and was critical for successful transformation. Hygromycin phosphotransferase (hpt) proved to be a good selectable marker for sorghum. We also found that β-glucuronidase (GUS) activity was low in most of the transgenic plant tissues tested, although it was very high in immature inflorescences. Although promising, the overall transformation efficiency of the protocol is still low and further optimization will require particular attention to be given to the number of Agrobacterium in the inoculu... Mostrar Tudo |
Thesagro: |
Sorgo. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32593/1/Agrobacterium-mediated.pdf
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Marc: |
LEADER 02225naa a2200205 a 4500 001 1488374 005 2015-11-23 008 2004 bl uuuu u00u1 u #d 100 1 $aCARVALHO, C. H. S. 245 $aAgrobacterium-mediated transformation of sorghum$bfactors that affect transformation efficiency.$h[electronic resource] 260 $c2004 520 $aThe results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving transformation efficiency. The addition of coconut water to the co-cultivation medium, the use of vigorous and actively growing immature embryos and the removal of excess bacteria significantly improved the survival rate of sorghum embryos and was critical for successful transformation. Hygromycin phosphotransferase (hpt) proved to be a good selectable marker for sorghum. We also found that β-glucuronidase (GUS) activity was low in most of the transgenic plant tissues tested, although it was very high in immature inflorescences. Although promising, the overall transformation efficiency of the protocol is still low and further optimization will require particular attention to be given to the number of Agrobacterium in the inoculum and the selection of sorghum genotypes and explants less sensitive to Agrobacterium infection. 650 $aSorgo 700 1 $aZEHR, U. B. 700 1 $aGUNARATNA, N. 700 1 $aANDERSON, J. 700 1 $aKONONOWICZ, H. H. 700 1 $aHODGES, T. K. 700 1 $aAXTELL, J. D. 773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 27, n. 2, p. 259-269, 2004.
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Embrapa Milho e Sorgo (CNPMS) |
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