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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
05/01/2021 |
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Data da última atualização: |
17/02/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CURSINO, A. E.; LIMA, M. T.; NOGUEIRA, M. F.; AGUIAR, D. M. de; LUIZ, A. P. M. F.; ALVES, P. A.; ARAUJO JUNIOR, J. P.; KROON, E. G. |
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Afiliação: |
ANDREIA ELISA CURSINO, Universidade Federal de Minas Gerais; MAURÍCIO TEIXEIRA LIMA, Universidade Federal de Minas Gerais; MARCIA FURLAN NOGUEIRA T DE LIMA, CPAP; DANIEL MOURA DE AGUIAR, Universidade Federal de Mato Grosso; ANA PAULA MOREIRA FRANCO LUIZ, Viriontech do Brasil Indústria de Insumos e Serviços em Biotecnologia; PEDRO AUGUSTO ALVES, Instituto René Rachou, Fiocruz; JOÃO PESSOA ARAUJO JUNIOR, Universidade Estadual Paulista (Unesp); ERNA GEESSIEN KROON, Universidade Federal de Minas Gerais. |
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Título: |
Identification of large genetic variations in the equine infectious anemia virus tat-gag genomic region. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Transboundary and Emerging Diseases, v. 68, n. 6, p. 3424-3432, 2021. |
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DOI: |
10.1111/tbed.13946 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The aetiological agent of equine infectious anaemia (EIA) is the retrovirus equine infectious anemia virus (EIAV) that infects all members of the Equidae family. The EIA is widely disseminated in the Brazilian territory with a high seroprevalence in the Brazilian Pantanal and is mainly diagnosed using agar gel immunodiffusion (AGID). There are few complete EIAV genome sequences available in GenBank, which had an impact on molecular detection studies. In this study, we conducted molecular detection and sequencing of EIAV proviral DNA from Brazilian horses. We analysed the genomic region from exon 1 of tat to gag (tat-gag). Comparative serological tests, comprising AGID and two enzyme-linked immunosorbent assays (ELISAs), were also conducted. Of the 133 samples, 58 were positive in the tat-gag PCR, and 49 nucleotide sequences of 272 bp were obtained. Using this developed tat-gag PCR EIAV proviral DNA was detected in 7% of the AGID-negative samples and 26% of the AGID-negative samples were positive in at least one of the ELISA tests used. Using phylogenetic analysis, the Brazilian Pantanal EIAV sequences grouped in a different clade of EIAV sequences from other countries. Thus, the EIAV sequences can contribute to the knowledge of the tat-gag genomic region in the circulating viruses in the Brazilian Pantanal, in addition to providing new information about the genetic diversity. In addition, the serological results demonstrate the greater sensitivity of the ELISAs used in this study compared to AGID for EIA diagnosis. MenosAbstract: The aetiological agent of equine infectious anaemia (EIA) is the retrovirus equine infectious anemia virus (EIAV) that infects all members of the Equidae family. The EIA is widely disseminated in the Brazilian territory with a high seroprevalence in the Brazilian Pantanal and is mainly diagnosed using agar gel immunodiffusion (AGID). There are few complete EIAV genome sequences available in GenBank, which had an impact on molecular detection studies. In this study, we conducted molecular detection and sequencing of EIAV proviral DNA from Brazilian horses. We analysed the genomic region from exon 1 of tat to gag (tat-gag). Comparative serological tests, comprising AGID and two enzyme-linked immunosorbent assays (ELISAs), were also conducted. Of the 133 samples, 58 were positive in the tat-gag PCR, and 49 nucleotide sequences of 272 bp were obtained. Using this developed tat-gag PCR EIAV proviral DNA was detected in 7% of the AGID-negative samples and 26% of the AGID-negative samples were positive in at least one of the ELISA tests used. Using phylogenetic analysis, the Brazilian Pantanal EIAV sequences grouped in a different clade of EIAV sequences from other countries. Thus, the EIAV sequences can contribute to the knowledge of the tat-gag genomic region in the circulating viruses in the Brazilian Pantanal, in addition to providing new information about the genetic diversity. In addition, the serological results demonstrate the greater sensitivity of the ELISAs use... Mostrar Tudo |
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Thesagro: |
Anemia Infecciosa; Cavalo. |
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Thesaurus Nal: |
Equine infectious anemia virus; Horses. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02360naa a2200265 a 4500
001 2128970
005 2022-02-17
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1111/tbed.13946$2DOI
100 1 $aCURSINO, A. E.
245 $aIdentification of large genetic variations in the equine infectious anemia virus tat-gag genomic region.$h[electronic resource]
260 $c2020
520 $aAbstract: The aetiological agent of equine infectious anaemia (EIA) is the retrovirus equine infectious anemia virus (EIAV) that infects all members of the Equidae family. The EIA is widely disseminated in the Brazilian territory with a high seroprevalence in the Brazilian Pantanal and is mainly diagnosed using agar gel immunodiffusion (AGID). There are few complete EIAV genome sequences available in GenBank, which had an impact on molecular detection studies. In this study, we conducted molecular detection and sequencing of EIAV proviral DNA from Brazilian horses. We analysed the genomic region from exon 1 of tat to gag (tat-gag). Comparative serological tests, comprising AGID and two enzyme-linked immunosorbent assays (ELISAs), were also conducted. Of the 133 samples, 58 were positive in the tat-gag PCR, and 49 nucleotide sequences of 272 bp were obtained. Using this developed tat-gag PCR EIAV proviral DNA was detected in 7% of the AGID-negative samples and 26% of the AGID-negative samples were positive in at least one of the ELISA tests used. Using phylogenetic analysis, the Brazilian Pantanal EIAV sequences grouped in a different clade of EIAV sequences from other countries. Thus, the EIAV sequences can contribute to the knowledge of the tat-gag genomic region in the circulating viruses in the Brazilian Pantanal, in addition to providing new information about the genetic diversity. In addition, the serological results demonstrate the greater sensitivity of the ELISAs used in this study compared to AGID for EIA diagnosis.
650 $aEquine infectious anemia virus
650 $aHorses
650 $aAnemia Infecciosa
650 $aCavalo
700 1 $aLIMA, M. T.
700 1 $aNOGUEIRA, M. F.
700 1 $aAGUIAR, D. M. de
700 1 $aLUIZ, A. P. M. F.
700 1 $aALVES, P. A.
700 1 $aARAUJO JUNIOR, J. P.
700 1 $aKROON, E. G.
773 $tTransboundary and Emerging Diseases$gv. 68, n. 6, p. 3424-3432, 2021.
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Registro original: |
Embrapa Pantanal (CPAP) |
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| Registros recuperados : 76 | |
| 41. |  | SAMANIEGO, R. D.; CAVALCANTE, R. V.; NOCITI, D. L. P.; PETZOLD, H. V.; ESNARRIAGA, E. S.; ARAÚJO JR. J. P.; NOGUEIRA, M. F. Equine infectious anemia diagnosis by Pcr, Elisa and Agid in urban equids from Corumbá and Ladário, MS, Brazil. In: BRAZILIAN CONGRESS OF VIROLOGY, 24.; MERCOSUR MEETING OF VIROLOGY, 8., 2013. Porto Seguro: SBV, 2013. p.231. Journal of the Brazilian Society for Virology, v. 18, suppl. 1, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 42. | | SANTOS, S. A.; SOUZA, G. da S. e; REZENDE, A. S. C.; ANDRADE, D. R. F. de; NOGUEIRA, M. F.; MELLITO FILHO, R.; TRIGO, P. Influência da temperatura superficial no desempenho funcional de Cavalos Pantaneiros soropositivos e soronegativos para Anemia Infecciosa Equina. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 4., 2016, Curitiba, PR. Recursos genéticos no Brasil: a base para o desenvolvimento sustentável. Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2016. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 43. | | BORGES, A. M. C. M.; SILVA, L. G.; NOGUEIRA, M. F.; OLIVEIRA, A. C. S.; SEGRI, N. J.; FERREIRA, F.; WITTER, R.; AGUIAR, D. M. The Brazilian Pantanal remains at a great risk of Equine Infectious Anemia. Virus Reviews & Research, v. 17, n. 2, p. 71-72, 2012. Supplement 1. Trabalho apresentado no XXIII Congresso Brasileiro de Virologia & VII Reunião do Mercosul de Virologia, Foz do Iguaçu, PR. VV1107.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
|  |
| 44. | | SANTOS, D. R. dos; REZENDE, A. S. C.; SANTOS, S. A.; NOGUEIRA, M. F.; TRIGO, P.; MELLITO FILHO, R.; SILVA, R. H. P.; SANTIAGO, J. M. Desempenho físico de equinos soropositivos e soronegativos para anemia infecciosa equina. In: SIMPÓSIO INTERNACIONAL DO CAVALO ATLETA, 7., Belo Horizonte. [Anais...]. [Belo Horizonte: UFMG], 2015. SIMCAV.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 45. | | TAVARES, F. N.; COSTA, E. V.; BURLANDY, F. M.; MURTA, M. M.; PELLEGRIN, A. O.; NOGUEIRA, M. F.; SILVA, E. E. da. Circulation of St Louis Encephalitis Virus (SLEV), Eastern Equine Encephalitis Virus (EEEV) and Western Equine Encephalitis Virus (WEEV) in equines of south Pantanal, Brasil. Virus: Reviews & Research. Journal of the Brazilian Society for Virology, v.13, sup.2, p.110, 2008. Resumos do XIX NATIONAL MEETING OF VIROLOGY, 2008, Caxambu.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 46. | | MORAES, L. C.; BATISTA, R. E. R.; PERES, I. A. H. F. S.; PELLEGRIN, A. O.; JULIANO, R. S.; NOGUEIRA, M. F. Características físicas da área para o desenvolvimento de mosquitos do gênero Culicoides presentes no Campo Experimental da Embrapa Pantanal- Nhumirim, Corumbá, MS In: EVENTO DE INICIAÇÃO CIENTÍFICA DO PANTANAL, 5.; SEMANA DA BIOLOGIA, 12., 2017, Corumbá. Resumos... Corumbá: Embrapa Pantanal, 2017. (Embrapa Pantanal. Documentos, 154). p. 14.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 47. | | MONTEZUMA, E. de S.; NOGUEIRA, M. F.; COSTA NETO, A. A. da; JULIANO, R. S.; SANTOS, C. J. S.; REIS, J. K. P. dos. Comparação dos testes ELISA rgp90 e IDGA para diagnóstico da AIE no Pantanal, MS. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA PANTANAL E UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL, 3., SEMANA DE BIOLOGIA, 8. 2009, Corumbá. Resumos...Corumbá: Embrapa Pantanal, 2009. (Embrapa Pantanal. Documentos, 101). p. 24| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 48. | | NOGUEIRA, M. F.; SANTOS, C. J. S.; OLIVEIRA, A. L. C.; MONTEZUMA, E. S.; JULIANO, R. S.; MARQUES, D. K. S.; REIS, J. K. P. Prevalence of equine infectious anemia in working equidae of Corumbá, Mato Grosso do Sul, Brazil. Virus Reviews and Research, v. 15, supl. 1, p. 198, oct. 2010. Trabalho apresentado no NATIONAL MEETING OF VIROLOGY, 21.; MERCOSUL MEETING OF VIROLOGY, 5.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 49. | | BORGES, A. M. C. M.; SILVA, L. G.; NOGUEIRA, M. F.; OLIVEIRA, A. C. S.; SEGRI, N. J.; FERREIRA, F.; WITTER, R.; AGUIAR, D. M. Prevalence and risk factors for Equine Infectious Anemia in Poconé municipality, northern Brazilian Pantanal. Research in Veterinary Science, v. 95, n. 1, p. 76-81, 2013. 6p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pantanal. |
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| 50. | | PAUVOLID CORRÊA, A.; FIGUEIREDO, L. T. M.; MORALES, M. A.; LEVIS, S.; COUTO-LIMA, D.; NOGUEIRA, M. F.; SCHATZMAYR, H. G. Serological evidence of cacipacore virus circulation in horses from Brazilian Pantanal. Virus Reviews and Research, v. 15, supl. 1, p. 264-265, oct. 2010. Trabalho apresentado no NATIONAL MEETING OF VIROLOGY, 21.; MERCOSUL MEETING OF VIROLOGY, 5.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 51. | | PAUVOLID-CORRÊA, A.; TAVARES, F. N.; COSTA, E. V. da; BURLANDY, F. M.; MURTA, M.; PELLEGRIN, A. O.; NOGUEIRA, M. F.; SILVA, E. E. da. Serologic evidence of the recent circulation of Saint Louis encephalitis virus and high prevalence of equine encephalitis viruses in horses in the Nhecolândia sub-region in South Pantanal, Central-West Brazil. Memórias do Instituto Oswaldo Cruz, v. 105, n.6, p.829-833, September, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pantanal. |
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| 53. | | PAUVOLID - CORREA, A.; MORALES, M. A.; LEVIS, S.; FIGUEIREDO, L. T. M.; COUTO-LIMA, D.; NOGUEIRA, M. F.; SILVA, E. E.; NOGUEIRA, R. M. R; SCHATZMAYR, H. G. Detection of neutralizing antibodies for west nile virus in resident horses from Brazilian Pantanal. Virus Reviews and Research, v. 15, supl. 1, p. 265, oct. 2010. Trabalho apresentado no NATIONAL MEETING OF VIROLOGY, 21.; MERCOSUL MEETING OF VIROLOGY, 5.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 54. | | NOGUEIRA, M. F.; COSTA NETO, A. A.; JULIANO, R. S.; SANTOS, C. J. S.; MONTEZUMA, E. S.; REIS, J. K. P. Elisa RGP90 as an alternative methodology to diagnose Equine Infectious Anemia in Southern Pantanal, Brazil. Virus Reviews & Research, Rio de Janeiro, v. 14, p. 211-212, 2009. Suplplement 1. Trabalho apresentado no XX Encontro Nacional de Virologia, Brasília, DF. Resumo 408.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 56. | | SANTOS, S. A.; PAIVA, S. R.; COMASTRI FILHO, J. A.; RAMOS, A. F.; JULIANO, R. S.; NOGUEIRA, M. F.; PERES, I. A. H. F. S. Histórico, vivências e desafios do núcleo de conservação do cavalo Pantaneiro. Revista Científica de Produção Animal, v. 22, n. 2, p. 67-72, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Pantanal; Embrapa Recursos Genéticos e Biotecnologia. |
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| 57. | | CURSINO, A. E.; LIMA, M. T.; NOGUEIRA, M. F.; AGUIAR, D. M. de; LUIZ, A. P. M. F.; ALVES, P. A.; ARAUJO JUNIOR, J. P.; KROON, E. G. Identification of large genetic variations in the equine infectious anemia virus tat-gag genomic region. Transboundary and Emerging Diseases, v. 68, n. 6, p. 3424-3432, 2021.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Pantanal. |
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| 58. | | MALOSSI, C. D.; CAVALCANTE, R. V.; ULLMANN, L. S.; NOGUEIRA, M. F.; AGUIAR, D. M.; KROON, E. G.; ARAUJO JR. J. P. Identification of a novel equine infectious anemia virus strain by massive sequencing in an endemic zone in Pantanal, Brazil. Journal of the Brazilian Society for Virology, v. 19, p.259-260, 2014. Supplement 2. BRAZILIAN CONGRESS OF VIROLOGY, 25.; MERCOSUR MEETING OF VIROLOGY, 9., 2014, Ribeirão Preto, SP.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| Registros recuperados : 76 | |
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