Data/hora: 07/06/2024 01:22:59

Biblioteca(s): | Embrapa Pecuária Sudeste.
Data corrente: | 07/06/2004
Data da última atualização: | 05/05/2022
Autoria: | VIEIRA, E. C.; NOGUEIRA, A. R. de A.
Afiliação: | EDIVAN CARVALHO VIEIRA, UFSCAR; ANA RITA DE ARAUJO NOGUEIRA, CPPSE.
Título: | Orthophosphate, phytate, and total phosohorus determination in cerals by flow injection analysis.
Ano de publicação: | 2004
Fonte/Imprenta: | Journal of Agricultural and Food Chemistry, v.52, p.1800-1803, 2004.
DOI: | https://doi.org/10.1021/jf034467f
Idioma: | Inglês
Conteúdo: | A flow injection spectrophotometric procedure with enzymatic hydrolysis was developed for determination of orthophosphate, phytate and total phosphorus in cereal samples. Phosphorus species were extracted from cereals with 0.05 mol L-1 potassium hydrogen phthalate buffer solution at pH 5.7. Orthophosphate was directly determined in the extracts by molybdenum blue spectrophotometric method. The phytate was hydrolyzed by the enzyme phytase coupled to a solid phase packed into an enzymatic reactor, and the resulting hydrolyzed orthophosphate was also determined by spectrophotometry at 650 nm. After optimization for phosphorus species extraction and enzymatic hydrolysis, a linear calibration graph was obtained up to 196 × 10-6 mol L-1 orthophosphate (P conc) -2.67 + 0.52x, r ) 0.9998). Measurements are characterized by relative standard deviation of 1.6% for a standard of 72 × 10-6 mol L-1 orthophosphate and no baseline drift was observed during 4 h operation periods. It provides 72 measurements per hour, with 2.4 × 10-6 mol L-1 and 7.9 × 10-6 mol L-1 as detection and quantification limits, respectively.
Palavras-Chave: | Acid; Enzymatic hysrolysis; Immobilization; Phytase; Phytic; Spectophometry.
Categoria do assunto: | W Química e Física
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/88222/1/PROCI-2004.00041.pdf
Registro original: | Embrapa Pecuária Sudeste (CPPSE)
Exemplares: | CPPSE - (15139-1) - UPC - AP - DD - PROCI-2004.00041 - VIE - 2004.00041 - DIS