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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
12/07/2006 |
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Data da última atualização: |
30/08/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DONATI, G. L.; NASCENTES, C. C.; NOGUEIRA, A. R. de A.; ARRUDA, M. A. Z.; NÓBREGA, J. A. |
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Título: |
Acid extraction and cloud point preconcentration as sample preparation strategies for cobalt determination in biological materials by thermospray flame furnace atomic absorption spectrometry. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Microchemical Journal, v.82, p.189-195, 2006. |
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Idioma: |
Inglês |
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Conteúdo: |
A thermospray flame furnace atomic absorption spectrometer (TS-FF-AAS) was employed for Co detennination in biological materiaIs. Cobalt presents a high atomization temperature and consequently poor sensitivity is obtained without changing its thennochemical behavior. The effect of different complexing agents on sensitivity was evaluated based on the fonnation of Co volatile compounds. A cloud point procedure was optimized for Co preconcentration for further improvement of sensitivity. Samples were treated with I moI I-I hydrochloric acid solution for quantitative extraction of Co without simultaneous extraction of Fe, since it is a strong interferent. After the extraction and preconcentration steps, a sample volume of 150 /lI was introduced into the hot Ni tube using air as carrier at a tlow-rate of 0.4 ml min- I. The best sensitivity was attained using ammonium pyrrolidinedithiocarbamate (APDC) and Triton X-114 was employed for implementation of the cloud point procedure. The detection limit obtained for Co was 2.1 /lg 1-1 and the standard deviation was 5.8% for a solution containing 100/lg I-I (n= 10). Accuracy was checked using two certified reference materiaIs (tomato leaves and bovine li ver) and results were in agreement with certified values at a 95% confidence leveI. Employing the developed procedure, Co were quantified in different biological materiaIs (plant and animal tissues). |
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Palavras-Chave: |
Acid extraction; Cloud point extraction; Flame atomic absorption spectrometry; Thermospray; Tube in flame. |
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Thesaurus Nal: |
cobalt. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02213naa a2200241 a 4500
001 1045669
005 2013-08-30
008 2006 bl uuuu u00u1 u #d
100 1 $aDONATI, G. L.
245 $aAcid extraction and cloud point preconcentration as sample preparation strategies for cobalt determination in biological materials by thermospray flame furnace atomic absorption spectrometry.
260 $c2006
520 $aA thermospray flame furnace atomic absorption spectrometer (TS-FF-AAS) was employed for Co detennination in biological materiaIs. Cobalt presents a high atomization temperature and consequently poor sensitivity is obtained without changing its thennochemical behavior. The effect of different complexing agents on sensitivity was evaluated based on the fonnation of Co volatile compounds. A cloud point procedure was optimized for Co preconcentration for further improvement of sensitivity. Samples were treated with I moI I-I hydrochloric acid solution for quantitative extraction of Co without simultaneous extraction of Fe, since it is a strong interferent. After the extraction and preconcentration steps, a sample volume of 150 /lI was introduced into the hot Ni tube using air as carrier at a tlow-rate of 0.4 ml min- I. The best sensitivity was attained using ammonium pyrrolidinedithiocarbamate (APDC) and Triton X-114 was employed for implementation of the cloud point procedure. The detection limit obtained for Co was 2.1 /lg 1-1 and the standard deviation was 5.8% for a solution containing 100/lg I-I (n= 10). Accuracy was checked using two certified reference materiaIs (tomato leaves and bovine li ver) and results were in agreement with certified values at a 95% confidence leveI. Employing the developed procedure, Co were quantified in different biological materiaIs (plant and animal tissues).
650 $acobalt
653 $aAcid extraction
653 $aCloud point extraction
653 $aFlame atomic absorption spectrometry
653 $aThermospray
653 $aTube in flame
700 1 $aNASCENTES, C. C.
700 1 $aNOGUEIRA, A. R. de A.
700 1 $aARRUDA, M. A. Z.
700 1 $aNÓBREGA, J. A.
773 $tMicrochemical Journal$gv.82, p.189-195, 2006.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Voltar
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| Acesso ao texto completo restrito à biblioteca da Embrapa Pesca e Aquicultura. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
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Data corrente: |
17/02/2012 |
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Data da última atualização: |
25/03/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
MELO, M. A. D.; SILVA, A. R. B da; VARELA, E. S.; SAMPAIO, I.; TAGLIARO, C. H. |
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Afiliação: |
MAURO ANDRÉ DAMASCENO MELO, INSTITUTO FEDERAL DO PARÁ; ADAM RICK BESSA DA SILVA, UNIVERSIDADE FEDERAL DO PARÁ; EDUARDO SOUSA VARELA, CNPASA; IRACILDA SAMPAIO, UNIVERSIDADE FEDERAL DO PARÁ; CLAUDIA HELENA TAGLIARO, UNIVERSIDADE FEDERAL DO PARÁ. |
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Título: |
Development and characterization of ten microsatellite markers for population studies of the native Brazilian oyster Crassostrea gasar. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Conservation Genetics Resources, v. 4, n. 3, p. 583-586, Sept. 2012. |
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Idioma: |
Português |
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Conteúdo: |
A set of ten polymorphic microsatellite loci were isolated and characterized from a CT/GT enriched genomic library of the Brazilian native oyster Crassostrea gasar (Adanson, 1757). These markers were polymorphic and successfully amplified in 21 individuals from São João de Pirabas, Pará state, Brazil. The number of alleles per polymorphic locus varied from 6 to 18 and values of observed and expected heterozygosity ranged from 0.3333 to 1.0000 and from 0.6655 to 0.9361, respectively. Only one of the ten microsatellite loci exhibited deviation from Hardy?Weinberg equilibrium and no evidence of linkage was detected between loci. These informative genetic markers will be very useful in population genetic studies of the mangrove oyster C. gasar. The ten markers also showed cross-species transferability with the native oyster Crassostrea rhizophorae. |
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Thesagro: |
Ostra. |
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Thesaurus NAL: |
Crassostrea; Genetic markers; Microsatellite repeats; Oysters. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01556naa a2200229 a 4500
001 1916161
005 2015-03-25
008 2012 bl uuuu u00u1 u #d
100 1 $aMELO, M. A. D.
245 $aDevelopment and characterization of ten microsatellite markers for population studies of the native Brazilian oyster Crassostrea gasar.
260 $c2012
520 $aA set of ten polymorphic microsatellite loci were isolated and characterized from a CT/GT enriched genomic library of the Brazilian native oyster Crassostrea gasar (Adanson, 1757). These markers were polymorphic and successfully amplified in 21 individuals from São João de Pirabas, Pará state, Brazil. The number of alleles per polymorphic locus varied from 6 to 18 and values of observed and expected heterozygosity ranged from 0.3333 to 1.0000 and from 0.6655 to 0.9361, respectively. Only one of the ten microsatellite loci exhibited deviation from Hardy?Weinberg equilibrium and no evidence of linkage was detected between loci. These informative genetic markers will be very useful in population genetic studies of the mangrove oyster C. gasar. The ten markers also showed cross-species transferability with the native oyster Crassostrea rhizophorae.
650 $aCrassostrea
650 $aGenetic markers
650 $aMicrosatellite repeats
650 $aOysters
650 $aOstra
700 1 $aSILVA, A. R. B da
700 1 $aVARELA, E. S.
700 1 $aSAMPAIO, I.
700 1 $aTAGLIARO, C. H.
773 $tConservation Genetics Resources$gv. 4, n. 3, p. 583-586, Sept. 2012.
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Embrapa Pesca e Aquicultura (CNPASA) |
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