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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
21/03/2024 |
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Data da última atualização: |
22/03/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
KAPRITCHKOFF, R. T. I.; OKINO, C. H.; NICIURA, S. C. M.; BELLO, H. J. S.; MATOS, R. S.; MELITO, G. R.; DONATONI, F. A. B.; ESTEVES, S. N.; CHAGAS, A. C. de S. |
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Afiliação: |
RAFAELA TAMI IKEDA KAPRITCHKOFF, Universidade Estadual Paulista; CINTIA HIROMI OKINO, CPPSE; SIMONE CRISTINA MEO NICIURA, CPPSE; HORNBLENDA JOAQUINA SILVA BELLO; RENATA SILVA MATOS; GLAUCIA ROBERTA MELITO; FLAVIA ALINE BRESSANI DONATONI, CPPSE; SERGIO NOVITA ESTEVES, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
Association of β-globin polymorphisms and tolerance to haemonchosis in ewes and lambs of different sheep breeds. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Veterinary Parasitology, v. 328, june 2024, 110163. |
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Páginas: |
8 p. |
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DOI: |
10.1016/j.vetpar.2024.110163 |
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Idioma: |
Inglês |
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Conteúdo: |
Gastrointestinal nematodes (GIN), especially Haemonchus contortus, represent a significant challenge for sheep production. Given the global concern about GIN anthelmintic resistance, alternative control methods able to reduce the dependence on these drugs are highly advisable. Since previous studies have shown that sheep carrying the Hb-A allele of β-globin are more resistant to H. contortus, this study aimed to investigate the relationship between the different haplotypes (Hb-AA, Hb-AB and Hb-BB) and phenotypes in Santa Inês (SI), Texel (TX) and White Dorper (DO) breeds infected with H. contortus. Blood samples were collected from 180 ewes and 123 lambs of the three breeds for DNA extraction followed by qPCR using a hydrolysis probe to identify the β-globin haplotypes. Phenotypic data, including fecal egg count (FEC), packed cell volume (PCV), FAMACHA score and body condition score for ewes and lambs, as well as weight gain for lambs, were collected. The genotypic frequencies of β-globin for ewes and lambs were, respectively: 21.7% and 21.4% Hb-AA, 50% and 50% Hb-AB and 28.3% and 28.6% Hb-BB in SI; 0% and 0% Hb-AA, 18.6% and 9.4% Hb-AB and 81.4% and 90.6% Hb-BB in TX; and 0% and 0% Hb-AA, 13.1% and 0% Hb-AB and 86.9% and 100% Hb-BB in DO. In ewes, mean PCV differed (p<0.05) between the three haplotypes, with higher PCV in Hb-AA animals, followed by Hb-AB and Hb-BB. When considering each breed separately, SI Hb-AA ewes presented higher PCV (p<0.05), highlighting that even in a breed already considered resistant, animals with Hb-AA haplotype showed superior performance. Lambs with the Hb-AA haplotype exhibited a higher (p<0.05) mean PCV compared to those with Hb-AB and Hb-BB. The same pattern was found in SI when analyzing each breed separately. No significant association was found between β-globin haplotypes and FEC, FAMACHA score, body condition score, or weight gain. Nevertheless, given that anemia is the major clinical sign of haemonchosis, our findings on PCV reinforce that sheep carrying the Hb-A allele of β-globin are more tolerant to haemonchosis. This study may support the development of a valuable tool, targeting genetic selection for GIN control, reducing the dependence on anthelmintics and boosting sheep production worldwide. MenosGastrointestinal nematodes (GIN), especially Haemonchus contortus, represent a significant challenge for sheep production. Given the global concern about GIN anthelmintic resistance, alternative control methods able to reduce the dependence on these drugs are highly advisable. Since previous studies have shown that sheep carrying the Hb-A allele of β-globin are more resistant to H. contortus, this study aimed to investigate the relationship between the different haplotypes (Hb-AA, Hb-AB and Hb-BB) and phenotypes in Santa Inês (SI), Texel (TX) and White Dorper (DO) breeds infected with H. contortus. Blood samples were collected from 180 ewes and 123 lambs of the three breeds for DNA extraction followed by qPCR using a hydrolysis probe to identify the β-globin haplotypes. Phenotypic data, including fecal egg count (FEC), packed cell volume (PCV), FAMACHA score and body condition score for ewes and lambs, as well as weight gain for lambs, were collected. The genotypic frequencies of β-globin for ewes and lambs were, respectively: 21.7% and 21.4% Hb-AA, 50% and 50% Hb-AB and 28.3% and 28.6% Hb-BB in SI; 0% and 0% Hb-AA, 18.6% and 9.4% Hb-AB and 81.4% and 90.6% Hb-BB in TX; and 0% and 0% Hb-AA, 13.1% and 0% Hb-AB and 86.9% and 100% Hb-BB in DO. In ewes, mean PCV differed (p<0.05) between the three haplotypes, with higher PCV in Hb-AA animals, followed by Hb-AB and Hb-BB. When considering each breed separately, SI Hb-AA ewes presented higher PCV (p<0.05), highlighting that even in... Mostrar Tudo |
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Palavras-Chave: |
Ovine; Parasite tolerance; Resilience. |
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Thesagro: |
Anemia; Haemonchus Contortus. |
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Thesaurus Nal: |
Genotyping; Haplotypes. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/263903/1/AssociationBGlobin.pdf
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Marc: |
LEADER 03233naa a2200325 a 4500
001 2163085
005 2024-03-22
008 2024 bl uuuu u00u1 u #d
024 7 $a10.1016/j.vetpar.2024.110163$2DOI
100 1 $aKAPRITCHKOFF, R. T. I.
245 $aAssociation of β-globin polymorphisms and tolerance to haemonchosis in ewes and lambs of different sheep breeds.$h[electronic resource]
260 $c2024
300 $a8 p.
520 $aGastrointestinal nematodes (GIN), especially Haemonchus contortus, represent a significant challenge for sheep production. Given the global concern about GIN anthelmintic resistance, alternative control methods able to reduce the dependence on these drugs are highly advisable. Since previous studies have shown that sheep carrying the Hb-A allele of β-globin are more resistant to H. contortus, this study aimed to investigate the relationship between the different haplotypes (Hb-AA, Hb-AB and Hb-BB) and phenotypes in Santa Inês (SI), Texel (TX) and White Dorper (DO) breeds infected with H. contortus. Blood samples were collected from 180 ewes and 123 lambs of the three breeds for DNA extraction followed by qPCR using a hydrolysis probe to identify the β-globin haplotypes. Phenotypic data, including fecal egg count (FEC), packed cell volume (PCV), FAMACHA score and body condition score for ewes and lambs, as well as weight gain for lambs, were collected. The genotypic frequencies of β-globin for ewes and lambs were, respectively: 21.7% and 21.4% Hb-AA, 50% and 50% Hb-AB and 28.3% and 28.6% Hb-BB in SI; 0% and 0% Hb-AA, 18.6% and 9.4% Hb-AB and 81.4% and 90.6% Hb-BB in TX; and 0% and 0% Hb-AA, 13.1% and 0% Hb-AB and 86.9% and 100% Hb-BB in DO. In ewes, mean PCV differed (p<0.05) between the three haplotypes, with higher PCV in Hb-AA animals, followed by Hb-AB and Hb-BB. When considering each breed separately, SI Hb-AA ewes presented higher PCV (p<0.05), highlighting that even in a breed already considered resistant, animals with Hb-AA haplotype showed superior performance. Lambs with the Hb-AA haplotype exhibited a higher (p<0.05) mean PCV compared to those with Hb-AB and Hb-BB. The same pattern was found in SI when analyzing each breed separately. No significant association was found between β-globin haplotypes and FEC, FAMACHA score, body condition score, or weight gain. Nevertheless, given that anemia is the major clinical sign of haemonchosis, our findings on PCV reinforce that sheep carrying the Hb-A allele of β-globin are more tolerant to haemonchosis. This study may support the development of a valuable tool, targeting genetic selection for GIN control, reducing the dependence on anthelmintics and boosting sheep production worldwide.
650 $aGenotyping
650 $aHaplotypes
650 $aAnemia
650 $aHaemonchus Contortus
653 $aOvine
653 $aParasite tolerance
653 $aResilience
700 1 $aOKINO, C. H.
700 1 $aNICIURA, S. C. M.
700 1 $aBELLO, H. J. S.
700 1 $aMATOS, R. S.
700 1 $aMELITO, G. R.
700 1 $aDONATONI, F. A. B.
700 1 $aESTEVES, S. N.
700 1 $aCHAGAS, A. C. de S.
773 $tVeterinary Parasitology$gv. 328, june 2024, 110163.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 172 | |
| 5. |  | NICIURA, S. C. M. Genomic imprinting e suas implicações em produção animal. In: SIMPÓSIO DE BIOLOGIA MOLECULAR APLICADA À PRODUÇÃO ANIMAL, 1., 2007, São Carlos. São Carlos: Embrapa Pecuária Sudeste, 2007. v.1 p.52-66 1 CD-ROM Editado por Luciana Correia de Almeida Regitano, Simone C. Méo.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 7. | | NICIURA, S. C. M. Protocolos genotípicos para nematoides gastrintestinais. In: CHAGAS, A. C. de S.; NICIURA, S. C. M.; MOLENTO, M. B. (Ed.). Manual prático: metodologias de diagnóstico da resistências e de detecção de sustâncias ativas em parasitas de ruminantes. Brasília, DF: Embrapa Informação Tecnológica, 2011. p. 59-82.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 8. | | NICIURA, S. C. M.; THOLON, P. Absence of CpG observed/expected ratio depletion in Haemonchus contortus evidences a non-methylated genome. In: WORKSHOP ON OMICS STRATEGIES APPLIED TO LIVESTOCK SCIENCE, 1., 2017, Piracicaba, SP. Proceedings... São Carlos, SP: Embrapa Pecuária Sudeste, 2017. p. 21. (Embrapa Pecuária Sudeste. Documentos, 125) Editores: Luiz Lehmann Coutinho, ESALQ/USP; Luciana Correia de Almeida Regitano, Embrapa Pecuária Sudeste; Gerson Barreto Mourão, ESALQ/USP; Aline Silva Mello Cesar, ESALQ/USP; Bárbara Silva Vignato, FZEA/USP; Mirele Daiana Poleti,...| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 11. | | NICIURA, S. C. M.; PERECIN, F.; SARAIVA, N. Z. Controle epigenético do desenvolvimento e do envelhecimento em mamíferos. In: NICIURA, S. C. M.; SARAIVA, N. Z. (Ed.). Epigenética: bases moleculares, efeitos na fisiologia e na patologia, e implicações para a produção animal e a vegetal. Brasília, DF: Embrapa, 2014. Cap. 7, p. 155-187.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 12. | | CRUVINEL, G. G.; MORAES, C. V.; NICIURA, S. C. M. Variabilidade genética para marcadores SNP associados à resistência a Haemonchus contortus em ovinos Morada Nova. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 10., 2018, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação; Embrapa Pecuária Sudeste, 2018. p. 19. (Embrapa Instrumentação. Documentos, 68). Editores técnicos: Daniel Souza Corrêa, Elaine Cristina Paris, Maria Alice Martins, Paulino Ribeiro Villas Boas, Wilson Tadeu Lopes da Silva.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 16. | | CRUVINEL, G. G.; MORAES, C. V.; NICIURA, S. C. M. Genotipagem de marcadores moleculares de resistência ao parasita Haemonchus contortus em ovinos da raça Morada Nova. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 9., 2017, São Carlos, SP. Anais... São Carlos, SP: Embrapa Pecuária Sudeste; Embrapa Instrumentação, 2017. p. 43. (Embrapa Pecuária Sudeste. Documentos, 126). Editores técnicos: Alexandre Berndt, Ana Rita Araujo Nogueira, Bianca Baccili Zanotto Vigna, Juliana Gonçalves Costa, Lea Chapaval, Manuel Antonio Chagas Jacinto, Patricia Menezes Santos.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 17. | | MORAES, C. V.; CRUVINEL, G. G.; NICIURA, S. C. M. Investigação da ocorrência de eventos epigenéticos em Haemonchus contortus em estudo da resistência ao monepantel. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 9., 2017, São Carlos, SP. Anais... São Carlos, SP: Embrapa Pecuária Sudeste; Embrapa Instrumentação, 2017. p. 18. (Embrapa Pecuária Sudeste. Documentos, 126). Editores técnicos: Alexandre Berndt, Ana Rita Araujo Nogueira, Bianca Baccili Zanotto Vigna, Juliana Gonçalves Costa, Lea Chapaval, Manuel Antonio Chagas Jacinto, Patricia Menezes Santos.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 172 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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