Data/hora: 04/06/2024 06:32:42

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 26/01/1996
Data da última atualização: | 26/01/1996
Autoria: | HILF, M. E.; KARASEV, A. V.; PAPPU, H. R.; GUMPF, D. J.; NIBLETT, C. L.; GARNSEY, S.
Afiliação: | USDA-ARS Horticultural Research Laboratory, 2120 Camden Road, Orlando,Florida 32803; Department of Plant Pathology, University of California, Riverside, California 92521-0122; and Plant Pathology Department.
Título: | Characterization of citrus tristeza virus subgenomic RNAs in infected Tissue.
Ano de publicação: | 1995
Fonte/Imprenta: | Virology v.208, p.576-582, 1995.
Idioma: | Inglês
Conteúdo: | Citrus tristeza virus (CTV) specific RNAs extracted from infected citrus tissue were analyzed by Northerm blot hybridization. TNAs were charcterized by size and identifield using cDNA probes specific to nine open reading frames (ORFs) identified by the analysis of sequence obtained from cDNA clones of the T36 isolate of CTV. Sequence specific cDNA probes identified the genomic RNA as well as subgenomic RNAs representing the p33, p65, p61, p27, p25, p18, p13, p20, and p23, ORFs in extracts of total or double-stranded RNA (dsRNA) isolated from infected tissue. A probe derived from the 3' terminal ORF (p23) hybridized to eachof these subgenomic RNAs, indicating that the RNAs are 3' coterminal. Thwe relative amounts of the different subgenimic RNAs varied widely. The RNAs for the p20 and p23 ORFs were the most abundant and surpassed the amount of the p25 or capside protein specific subgenomic RNA. The number and sizes pf the CTV sobgenomic RNAs were the same in total and dsRNA preparations. Propagation of T36 in seven different citrus hosts did not alter the pattem of subgenomic RNAs.
Palavras-Chave: | Diseases; Doencas; Propagacao; Propagation.
Thesagro: | Vírus.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (3610-1) - ADC - AP - -- - 576.6405 -  - 576.6405 - DIS