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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
27/10/2010 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
ZAROS, L. G.; COUTINHO, L. L.; SIDER, L. H.; MEDEIROS, H. R. de; NEVES, M. R. M. das; BENVENUTI, C. L.; NAVARRO, A. M. do C.; VIEIRA, L. da S. |
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Afiliação: |
LILIAN GIOTTO ZAROS, Universidade Federal do Rio Grande do Norte, Natal, RN; LUIZ LEHMANN COUTINHO, ESALQ-USP, Piracicaba, SP; LUCIA HELENA SIDER, CNPC; HENRIQUE ROCHA DE MEDEIROS, Universidade Federal do Rio Grande do Norte, Natal, RN; MARIA ROSALBA MOREIRA DAS NEVES, Universidade Estadual Vale do Acaraú (UVA), Sobral, CE.; CAMILA LOURDES BENVENUTI, Universidade Estadual Vale do Acaraú (UVA), Sobral, CE.; ANDRINE MARIA DO CARMO NAVARRO, Universidade Estadual Vale do Acaraú (UVA), Sobral, CE.; LUIZ DA SILVA VIEIRA, CNPC. |
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Título: |
Evaluation of reference genes for real-time PCR studies of Brazilian Somalis sheep infected by gastrointestinal nematodes. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, v. 33, n. 3, p. 486-490, 2010. |
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Idioma: |
Inglês |
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Notas: |
Short communication. |
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Conteúdo: |
Abstract: Precise normalization with reference genes is necessary, in order to obtain reliable relative expression data in response to gastrointestinal nematode infection. By using sheep from temperate regions as models, three reference genes, viz., ribosomal protein LO (RPLO), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and succinate dehydrogenase complex subunit A (SDHA), were investigated in the abomasum, abomasal lymph nodes and small intestine of Brazilian Somalis sheep, either resistant or susceptible to gastrointestinal nematodes infections. Real time PCR was carried out by using SYBR Green I dye, and gene stability was tested by geNorm. RPLO was an ideal reference gene, since its expression was constant across treatments, presented lower variation, and was ranked as the most stable in abomasum and lymph node tissues. On the other hand, SDHA was the most stable in the small intestine followed by RPLO and GAPDH. These findings demonstrate the importance of correctly choosing reference genes prior to relative quantification. In addition, we determined that reference genes used in sheep from temperate regions, when properly tested, can be applied in animals from tropical regions such as the Brazilian Somalis sheep. |
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Palavras-Chave: |
Control genes; Gastrointestinal diseases; Nematódeo gastrintestinal; Nematodes; PCR em tempo real; Raça Somalis Brasileira; Real-time RT-PCR. |
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Thesagro: |
Doença animal; Helminto gastrintestinal; Nematóide; Ovino; Parasito de animal; Parasitologia. |
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Thesaurus Nal: |
Helminths; Nematode infections; Parasites; reverse transcriptase polymerase chain reaction; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/27007/1/NT-Evaluation-of-reference-genes-for-real-time-PCR-studies.pdf
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Marc: |
LEADER 02554naa a2200433 a 4500
001 1865496
005 2019-09-23
008 2010 bl uuuu u00u1 u #d
100 1 $aZAROS, L. G.
245 $aEvaluation of reference genes for real-time PCR studies of Brazilian Somalis sheep infected by gastrointestinal nematodes.$h[electronic resource]
260 $c2010
500 $aShort communication.
520 $aAbstract: Precise normalization with reference genes is necessary, in order to obtain reliable relative expression data in response to gastrointestinal nematode infection. By using sheep from temperate regions as models, three reference genes, viz., ribosomal protein LO (RPLO), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and succinate dehydrogenase complex subunit A (SDHA), were investigated in the abomasum, abomasal lymph nodes and small intestine of Brazilian Somalis sheep, either resistant or susceptible to gastrointestinal nematodes infections. Real time PCR was carried out by using SYBR Green I dye, and gene stability was tested by geNorm. RPLO was an ideal reference gene, since its expression was constant across treatments, presented lower variation, and was ranked as the most stable in abomasum and lymph node tissues. On the other hand, SDHA was the most stable in the small intestine followed by RPLO and GAPDH. These findings demonstrate the importance of correctly choosing reference genes prior to relative quantification. In addition, we determined that reference genes used in sheep from temperate regions, when properly tested, can be applied in animals from tropical regions such as the Brazilian Somalis sheep.
650 $aHelminths
650 $aNematode infections
650 $aParasites
650 $areverse transcriptase polymerase chain reaction
650 $aSheep
650 $aDoença animal
650 $aHelminto gastrintestinal
650 $aNematóide
650 $aOvino
650 $aParasito de animal
650 $aParasitologia
653 $aControl genes
653 $aGastrointestinal diseases
653 $aNematódeo gastrintestinal
653 $aNematodes
653 $aPCR em tempo real
653 $aRaça Somalis Brasileira
653 $aReal-time RT-PCR
700 1 $aCOUTINHO, L. L.
700 1 $aSIDER, L. H.
700 1 $aMEDEIROS, H. R. de
700 1 $aNEVES, M. R. M. das
700 1 $aBENVENUTI, C. L.
700 1 $aNAVARRO, A. M. do C.
700 1 $aVIEIRA, L. da S.
773 $tGenetics and Molecular Biology$gv. 33, n. 3, p. 486-490, 2010.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
17/11/2022 |
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Data da última atualização: |
17/11/2022 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
CAMPOLINO, M. L.; SANTOS, T. T.; LANA, U. G. de P.; GOMES, E. A.; GUILHEN, J. H. S.; PASTINA, M. M.; COELHO, A. M.; SOUSA, S. M. de. |
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Afiliação: |
MARIANA LOURENÇO CAMPOLINO, Universidade Federal de São João del-Rei; THIAGO TEIXEIRA SANTOS, CNPTIA; UBIRACI GOMES DE PAULA LANA, CNPMS; ELIANE APARECIDA GOMES, CNPMS; JOSÉ HENRIQUE SOLER GUILHEN; MARIA MARTA PASTINA, CNPMS; ANTONIO MARCOS COELHO, CNPMS; SYLVIA MORAIS DE SOUSA TINOCO, CNPMS. |
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Título: |
Crop type determines how root system architecture and microbial diversity indices relate in different phosphate fertilization conditions. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
In: PHOSPHORUS IN SOILS AND PLANTS SYMPOSIUM, 7., 2022, Montevideo. Towards a sustainable phosphorus utilization in agroecosystems: book of abstracts. Montevideo: Instituto Nacional de Investigación Agropecuaria, 2022. |
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Páginas: |
p. 31. |
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Idioma: |
Inglês |
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Conteúdo: |
This work aimed to generate and validade a more robust method of identifying root system architecture traits from maize and sorghum plants grown under different phosphate fertilization conditions and associate them with microbial genetic diversity indices and grain yield. |
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Palavras-Chave: |
Diversidade genética; Fertilização. |
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Thesagro: |
Adubação; Fosfato; Milho; Sistema Radicular; Sorgo. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 01297nam a2200289 a 4500
001 2148369
005 2022-11-17
008 2022 bl uuuu u01u1 u #d
100 1 $aCAMPOLINO, M. L.
245 $aCrop type determines how root system architecture and microbial diversity indices relate in different phosphate fertilization conditions.$h[electronic resource]
260 $aIn: PHOSPHORUS IN SOILS AND PLANTS SYMPOSIUM, 7., 2022, Montevideo. Towards a sustainable phosphorus utilization in agroecosystems: book of abstracts. Montevideo: Instituto Nacional de Investigación Agropecuaria$c2022
300 $ap. 31.
520 $aThis work aimed to generate and validade a more robust method of identifying root system architecture traits from maize and sorghum plants grown under different phosphate fertilization conditions and associate them with microbial genetic diversity indices and grain yield.
650 $aAdubação
650 $aFosfato
650 $aMilho
650 $aSistema Radicular
650 $aSorgo
653 $aDiversidade genética
653 $aFertilização
700 1 $aSANTOS, T. T.
700 1 $aLANA, U. G. de P.
700 1 $aGOMES, E. A.
700 1 $aGUILHEN, J. H. S.
700 1 $aPASTINA, M. M.
700 1 $aCOELHO, A. M.
700 1 $aSOUSA, S. M. de
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