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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
25/03/2013 |
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Data da última atualização: |
26/03/2013 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
GIROTTO, L.; SOLDERA, M. C. A.; MARIN, S. R. R.; KANAMORI, N.; FUJITA, Y.; YOSHIDA, T.; MARCELINO-GUIMARÃES, F. C.; YAMAGUCHI-SHINOZAKI; NEPOMUCENO, A. L. |
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Afiliação: |
SILVANA REGINA ROCKENBACH MARIN, CNPSO; FRANCISMAR CORREA MARCELINO, CNPSO; ALEXANDRE LIMA NEPOMUCENO, SRI. |
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Título: |
Agrobacterium-mediated transformation of soybean variated, BR16, gene 35S:AREB1, analysis by southern blotting. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: INTERNATIONAL CROP SCIENCE CONGRESS, 6., 2012, Bento Gonçalves. [Proceedings...]. [S.l.]: International Crop Science Society, 2012. Poster, 117. 1 CD-ROM. |
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Idioma: |
Inglês |
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Conteúdo: |
Soybean is the most important source of vegetable oil and protein for human and animal diet in the world. The frequency of occurrence of drought has increased significantly over the last decade, probably associated with climate change occurred due to global warming. Researchers are looking
for a long time to improve and optimize the characteristics of the culture. Several strategies can be used to reduce losses caused by drought, ranging from adequate soil management and agriculture, to the use of irrigation. Another possibility is the development of cultivars adapted to drought
conditions and in this respect, Biotechnology has become an important tool in the development of varieties adapted to different conditions of biotic and abiotic stress. The genetically modified plants can be transformed by direct gene transfer using particle bombardment (biolistics), which inserts a
high number of copies of the transgene into the host genome, resulting many times in deactivation of the transgene by gene silencing. Moreover, the transformed plants via Agrobacterium tumefaciens generally have a low number of copies of the transgene. The use of this method allowed a stable expression and insertion of the interest gene in transgenic plants. The AREB is an important transcription factor signaled by the ABA, which acts in response to regulate the expression of other genes that are involved in tolerance to drought and high salinity stress. This study aimed to analyze the T1 plants transformed Agrobacterium by Southern blotting technique to detect the number of copies of the gene 35S:AREB1. The experiment was conducted at the Laboratory of Plant Biotechnology at Embrapa Soja, Londrina - PR. The process was performed according Kanamori et al., 2011. The Southern blotting was performed on 22 plants T1, AREB positive from two independent events. Of the 22 samples, 20 T1 plants had only one copy number and two plants had two copy numbers. Considering the complexity of the mechanism response to water deficit caused by drought, which can vary depending on the intensity and duration of stress, analysis on the molecular level, physiological and agronomic are needed later to assess the efficiency of the presence of the gene in transformed plants. MenosSoybean is the most important source of vegetable oil and protein for human and animal diet in the world. The frequency of occurrence of drought has increased significantly over the last decade, probably associated with climate change occurred due to global warming. Researchers are looking
for a long time to improve and optimize the characteristics of the culture. Several strategies can be used to reduce losses caused by drought, ranging from adequate soil management and agriculture, to the use of irrigation. Another possibility is the development of cultivars adapted to drought
conditions and in this respect, Biotechnology has become an important tool in the development of varieties adapted to different conditions of biotic and abiotic stress. The genetically modified plants can be transformed by direct gene transfer using particle bombardment (biolistics), which inserts a
high number of copies of the transgene into the host genome, resulting many times in deactivation of the transgene by gene silencing. Moreover, the transformed plants via Agrobacterium tumefaciens generally have a low number of copies of the transgene. The use of this method allowed a stable expression and insertion of the interest gene in transgenic plants. The AREB is an important transcription factor signaled by the ABA, which acts in response to regulate the expression of other genes that are involved in tolerance to drought and high salinity stress. This study aimed to analyze the T1 plants transform... Mostrar Tudo |
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Thesagro: |
Biotecnologia; Soja. |
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Thesaurus Nal: |
Biotechnology; Soybeans. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03123naa a2200265 a 4500
001 1954079
005 2013-03-26
008 2012 bl uuuu u00u1 u #d
100 1 $aGIROTTO, L.
245 $aAgrobacterium-mediated transformation of soybean variated, BR16, gene 35S$bAREB1, analysis by southern blotting.
260 $c2012
520 $aSoybean is the most important source of vegetable oil and protein for human and animal diet in the world. The frequency of occurrence of drought has increased significantly over the last decade, probably associated with climate change occurred due to global warming. Researchers are looking for a long time to improve and optimize the characteristics of the culture. Several strategies can be used to reduce losses caused by drought, ranging from adequate soil management and agriculture, to the use of irrigation. Another possibility is the development of cultivars adapted to drought conditions and in this respect, Biotechnology has become an important tool in the development of varieties adapted to different conditions of biotic and abiotic stress. The genetically modified plants can be transformed by direct gene transfer using particle bombardment (biolistics), which inserts a high number of copies of the transgene into the host genome, resulting many times in deactivation of the transgene by gene silencing. Moreover, the transformed plants via Agrobacterium tumefaciens generally have a low number of copies of the transgene. The use of this method allowed a stable expression and insertion of the interest gene in transgenic plants. The AREB is an important transcription factor signaled by the ABA, which acts in response to regulate the expression of other genes that are involved in tolerance to drought and high salinity stress. This study aimed to analyze the T1 plants transformed Agrobacterium by Southern blotting technique to detect the number of copies of the gene 35S:AREB1. The experiment was conducted at the Laboratory of Plant Biotechnology at Embrapa Soja, Londrina - PR. The process was performed according Kanamori et al., 2011. The Southern blotting was performed on 22 plants T1, AREB positive from two independent events. Of the 22 samples, 20 T1 plants had only one copy number and two plants had two copy numbers. Considering the complexity of the mechanism response to water deficit caused by drought, which can vary depending on the intensity and duration of stress, analysis on the molecular level, physiological and agronomic are needed later to assess the efficiency of the presence of the gene in transformed plants.
650 $aBiotechnology
650 $aSoybeans
650 $aBiotecnologia
650 $aSoja
700 1 $aSOLDERA, M. C. A.
700 1 $aMARIN, S. R. R.
700 1 $aKANAMORI, N.
700 1 $aFUJITA, Y.
700 1 $aYOSHIDA, T.
700 1 $aMARCELINO-GUIMARÃES, F. C.
700 1 $aYAMAGUCHI-SHINOZAKI
700 1 $aNEPOMUCENO, A. L.
773 $tIn: INTERNATIONAL CROP SCIENCE CONGRESS, 6., 2012, Bento Gonçalves. [Proceedings...]. [S.l.]: International Crop Science Society, 2012. Poster, 117. 1 CD-ROM.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja; Embrapa Tabuleiros Costeiros. |
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Data corrente: |
21/09/2010 |
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Data da última atualização: |
09/08/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
LIRA, M. A.; CARVALHO, H. W. L. de; CARVALHO, C. G. P.; LIMA, J. M. P. de. |
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Afiliação: |
MARCELO ABDON LIRA, EMPARN; HELIO WILSON LEMOS DE CARVALHO, CPATC; CLAUDIO GUILHERME PORTELA CARVALHO, CNPSO; JOÃO MARIA PINHEIRO DE LIMA, EMPARN. |
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Título: |
Desempenho de cultivares de girassol (Helianthus annuus. L.) no estado do Rio Grande do Norte. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MAMONA, 4., 2010, João Pessoa. Campina Grande: Embrapa Algodão, 2010. 1 CD-ROM. |
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Páginas: |
p. 1700 |
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Descrição Física: |
Artigo em anais. MEG 06 pdf. |
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Idioma: |
Português |
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Conteúdo: |
Este trabalho teve como objetivo avaliar o comportamento produtivo de diversas cultivares de girassol. O ensaio foi instalado no Município de Canguaretama/RN no ano agrícola de 2007( 6º22 ? S, 35º 07? W, altitude de 5m, temperatura média anual de 25 ºC O solo é classificado como latossolo amarelo de textura arenosa. A precipitação ocorrida durante a condução do ensaio foi de 904,6 mm. O delineamento experimental foi blocos ao acaso, com quatro repetições. Fez-se uma adubação utilizando-se a fórmula: 60-80-60 kg/ha mais 2 kg/ha de boro.Os materiais avaliados mostraram-se precoces, com uma média de 39 dias para atingir o florescimento inicial. Os rendimentos médios de grãos das cultivares ficaram entre 1.356 kg/ha e 2.581 kg/ha, com média geral de 1.829 kg/ha.Três cultivares apresentaram rendimentos médios de grãos acima de 2.200 kg/ha, sobressaindo-se os materiais M 734, HLA 863 e GIRA 19 e com o teor de óleo superior a 42 %. |
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Palavras-Chave: |
Cultivar; Genética de planta. |
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Thesagro: |
Girassol; Helianthus Annuus; Melhoramento; Variedade. |
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Thesaurus NAL: |
Variety trials. |
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Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/862613/1/MEG-06.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/30274/1/Marcelo-Lira-CBmamona.pdf
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Marc: |
LEADER 01739nam a2200241 a 4500
001 1862613
005 2023-08-09
008 2010 bl uuuu u00u1 u #d
100 1 $aLIRA, M. A.
245 $aDesempenho de cultivares de girassol (Helianthus annuus. L.) no estado do Rio Grande do Norte.
260 $aIn: CONGRESSO BRASILEIRO DE MAMONA, 4., 2010, João Pessoa. Campina Grande: Embrapa Algodão, 2010. 1 CD-ROM.$c2010
300 $ap. 1700$cArtigo em anais. MEG 06 pdf.
520 $aEste trabalho teve como objetivo avaliar o comportamento produtivo de diversas cultivares de girassol. O ensaio foi instalado no Município de Canguaretama/RN no ano agrícola de 2007( 6º22 ? S, 35º 07? W, altitude de 5m, temperatura média anual de 25 ºC O solo é classificado como latossolo amarelo de textura arenosa. A precipitação ocorrida durante a condução do ensaio foi de 904,6 mm. O delineamento experimental foi blocos ao acaso, com quatro repetições. Fez-se uma adubação utilizando-se a fórmula: 60-80-60 kg/ha mais 2 kg/ha de boro.Os materiais avaliados mostraram-se precoces, com uma média de 39 dias para atingir o florescimento inicial. Os rendimentos médios de grãos das cultivares ficaram entre 1.356 kg/ha e 2.581 kg/ha, com média geral de 1.829 kg/ha.Três cultivares apresentaram rendimentos médios de grãos acima de 2.200 kg/ha, sobressaindo-se os materiais M 734, HLA 863 e GIRA 19 e com o teor de óleo superior a 42 %.
650 $aVariety trials
650 $aGirassol
650 $aHelianthus Annuus
650 $aMelhoramento
650 $aVariedade
653 $aCultivar
653 $aGenética de planta
700 1 $aCARVALHO, H. W. L. de
700 1 $aCARVALHO, C. G. P.
700 1 $aLIMA, J. M. P. de
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Embrapa Tabuleiros Costeiros (CPATC) |
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