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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
11/04/2000 |
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Data da última atualização: |
15/10/2004 |
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Autoria: |
NEPOMUCENO, A. L.; NEUMAIER, N.; FARIAS, J. R. B.; CASAGRANDE, E. |
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Título: |
Gene expression in brazilian soybean genotypes tolerant to water deficit. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 6., 1999, Chicago. Proceedings: invited and contributed papers and posters. Chicago: University of Illinois / Soybean Research & Development Council, 1999. |
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Páginas: |
p.439. |
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Idioma: |
Inglês |
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Conteúdo: |
Abiotic stresses such as drought can significantly reduce crop yields and restrict the areas on which crops can be cultivated. The implications of this are tremendous since not only are producers affected but the entire society also. The development of new cultivars more tolerant to water deficits is one of the options to attenuate this problem. However, tolerance to drought in plants clearly is not a simple trait, but a complex of mechanisms working in combination to avoid or to tolerate water deficits. All mechanisms that plants use to tolerate drought do have a molecular/genetic basis. Comparison of gene expression in different genotypes and treatments should provide underlying information needed to analyze the biological processes that control how organisms respond to stress situations. This work has been conducted with the main objective of identifying, cloning and sequencing genes differentially expressed during water deficit in Brazilian soybean genotypes tolerant to drought. Physiological characterization results showed that cultivars BR-4 and OCEPAR 4 have a higher tolerance for periods of drought when compared with cultivars BR-16 and BRAGG. These four soybean genotypes are now been analyzed with the use of Differential Display (DD) a technique that uses mRNA as template to obtain representative cDNAs by reverse transcription and polymerase chain reaction. Differentially expressed cDNAs are extracted from the gel, cloned and sequenced. Earlier work conducted with cotton identified 52 mRNA transcripts differentially expressed during drought. Some of these transcript sequences were transformed in probes to survey Embrapa soybean germplasm bank to see if transcripts of these same gene families are expressed in soybean during water deficit. The differentially expressed mRNA transcripts identified in soybean can also be used as probes to screen germplasm. Plant physiological studies on responses to water deficit, molecular mapping of traits related to drought tolerance and the development of drought tolerant genotypes might be helped by these results. Furthermore, some of the identified genes may show a high potential for plant transformation studies. MenosAbiotic stresses such as drought can significantly reduce crop yields and restrict the areas on which crops can be cultivated. The implications of this are tremendous since not only are producers affected but the entire society also. The development of new cultivars more tolerant to water deficits is one of the options to attenuate this problem. However, tolerance to drought in plants clearly is not a simple trait, but a complex of mechanisms working in combination to avoid or to tolerate water deficits. All mechanisms that plants use to tolerate drought do have a molecular/genetic basis. Comparison of gene expression in different genotypes and treatments should provide underlying information needed to analyze the biological processes that control how organisms respond to stress situations. This work has been conducted with the main objective of identifying, cloning and sequencing genes differentially expressed during water deficit in Brazilian soybean genotypes tolerant to drought. Physiological characterization results showed that cultivars BR-4 and OCEPAR 4 have a higher tolerance for periods of drought when compared with cultivars BR-16 and BRAGG. These four soybean genotypes are now been analyzed with the use of Differential Display (DD) a technique that uses mRNA as template to obtain representative cDNAs by reverse transcription and polymerase chain reaction. Differentially expressed cDNAs are extracted from the gel, cloned and sequenced. Earlier work conducted with c... Mostrar Tudo |
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Palavras-Chave: |
Brasil; Soybean; Tolerancia a seca. |
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Thesagro: |
Soja. |
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Thesaurus Nal: |
Brazil; soil water deficit. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02965naa a2200241 a 4500
001 1461655
005 2004-10-15
008 1999 bl uuuu u00u1 u #d
100 1 $aNEPOMUCENO, A. L.
245 $aGene expression in brazilian soybean genotypes tolerant to water deficit.
260 $c1999
300 $ap.439.
520 $aAbiotic stresses such as drought can significantly reduce crop yields and restrict the areas on which crops can be cultivated. The implications of this are tremendous since not only are producers affected but the entire society also. The development of new cultivars more tolerant to water deficits is one of the options to attenuate this problem. However, tolerance to drought in plants clearly is not a simple trait, but a complex of mechanisms working in combination to avoid or to tolerate water deficits. All mechanisms that plants use to tolerate drought do have a molecular/genetic basis. Comparison of gene expression in different genotypes and treatments should provide underlying information needed to analyze the biological processes that control how organisms respond to stress situations. This work has been conducted with the main objective of identifying, cloning and sequencing genes differentially expressed during water deficit in Brazilian soybean genotypes tolerant to drought. Physiological characterization results showed that cultivars BR-4 and OCEPAR 4 have a higher tolerance for periods of drought when compared with cultivars BR-16 and BRAGG. These four soybean genotypes are now been analyzed with the use of Differential Display (DD) a technique that uses mRNA as template to obtain representative cDNAs by reverse transcription and polymerase chain reaction. Differentially expressed cDNAs are extracted from the gel, cloned and sequenced. Earlier work conducted with cotton identified 52 mRNA transcripts differentially expressed during drought. Some of these transcript sequences were transformed in probes to survey Embrapa soybean germplasm bank to see if transcripts of these same gene families are expressed in soybean during water deficit. The differentially expressed mRNA transcripts identified in soybean can also be used as probes to screen germplasm. Plant physiological studies on responses to water deficit, molecular mapping of traits related to drought tolerance and the development of drought tolerant genotypes might be helped by these results. Furthermore, some of the identified genes may show a high potential for plant transformation studies.
650 $aBrazil
650 $asoil water deficit
650 $aSoja
653 $aBrasil
653 $aSoybean
653 $aTolerancia a seca
700 1 $aNEUMAIER, N.
700 1 $aFARIAS, J. R. B.
700 1 $aCASAGRANDE, E.
773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 6., 1999, Chicago. Proceedings: invited and contributed papers and posters. Chicago: University of Illinois / Soybean Research & Development Council, 1999.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 623 | |
| 123. |  | KANAMORI, N.; FUGANTI, R.; ENGELS, C.; GIROTTO, L.; MARUYAMA, K.; SHINOZAKI, K.; NEPOMUCENO, A. L. Molecular markers for drought stress response of soybean. In: CONGRESSO BRASILEIRO DE SOJA, 5.; MERCOSOJA 2009, Goiânia. Resumos... Londrina: Embrapa Soja, 2009. p. 106, trab. 179. Editado por Adilson de Oliveira Júnior, Odilon Ferreira Saraiva, Clara Beatriz Hoffmann Campo, César de Castro.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 126. | | OYA, T.; NEPOMUCENO, A. L.; NEUMAIER, N.; FARIAS, J. R. B.; TOBITA, S.; ITO, O. Drought tolerance characteristics of brazilian soybean cultivars - evaluation and characterization of drought tolerance of variousbBrazilian soybean cultivars in the field. Plant Production Science, Japan, v. 7, n. 2, p. 129-137, 2004.| Biblioteca(s): Embrapa Soja. |
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| 127. | | NEUMAIER, N.; FARIAS, J. R. B.; NEPOMUCENO, A. L.; DELATTRE, N. Duracao dos periodos pre-indutivo, indutivo e pos-indutivo em soja. In: REUNIAO DE PESQUISA DA SOJA DA REGIAO CENTRAL DO BRASIL, 20., 1998, Londrina. Ata e Resumos. Londrina: EMBRAPA-CNPSo, 1998. p.202-203. (EMBRAPA-CNPSo. Documentos, 121).| Biblioteca(s): Embrapa Soja. |
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| 131. | | CARRARA, R.; FRANÇA, C.; FRANCHINI, J. C.; DEBIASI, H.; NEPOMUCENO, A. L.; FARIAS, J. R. B. Desenvolvimento radicular da soja visando a tolerância à seca. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 4., 2009, Londrina. Resumos... Londrina: Embrapa Soja, 2009. p. 262-267. (Embrapa Soja. Documentos, 312). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 132. | | FRANCHINI, J. C.; DEBIASI, H.; FRANÇA, C.; CARRARA, R.; NEPOMUCENO, A. L.; FARIAS, J. R. B. Desenvolvimento radicular da soja visando tolerância à seca. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 32., 2009, Fortaleza. O solo e a produção de bioenergia: perspectivas e desafios: anais. [Viçosa, MG]: SBCS; Fortaleza: UFC, 2009. 4 p. Trab. 2586. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 133. | | FRANCHINI, J. C.; DEBIASI, H.; FRANÇA, C.; CARRARA, R.; NEPOMUCENO, A. L.; FARIAS, J. R. Desenvolvimento do sistema radicular da soja visando tolerância à seca. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 32., 2009, Fortaleza. O solo e a produção de bioenergia: perspectivas e desafios: resumos. [Viçosa, MG]: SBCS; Fortaleza: UFC, 2009. p. 393. Ref.: PT1874.| Biblioteca(s): Embrapa Soja. |
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| 136. | | BORDINGNON, J. R.; TSUSHIDA, T.; YAMAKI, K.; NEPOMUCENO, A. L.; FARIAS, J. R.; NEUMAIER, N. Effect of water deficiency in the isoflavone contents in brazilian soybean cultivars. In: INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 3., 2000, Tsukuba. 2000: dawn of the innovative era for soybeans - proceedings. Tsukuba: Korin Publishing, 2000. p.99-100.| Biblioteca(s): Embrapa Soja. |
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| 138. | | FARIAS, J. R. B.; NEPOMUCENO, A. L.; NEUMAIER, N.; TOBITA, S.; ALMEIDA, I. R. de. Restrições da disponibilidade hídrica à obtenção de elevados rendimentos de grãos de soja. In: CONGRESSO BRASILEIRO DE SOJA, 4., 2006, Londrina. Resumos... Londrina: Embrapa Soja, 2006. p. 32-33. Organizado por Odilon Ferreira Saraiva, Simone Ery Grosskopf.| Biblioteca(s): Embrapa Soja. |
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| 139. | | FARIAS, J. R. B.; NEUMAIER, N.; SINCLAIR, T. R.; NEPOMUCENO, A. L. Respostas de cultivares de soja ao termofotoperíodo. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 27., 2005. Cornélio Procópio. Resumos... Londrina: Embrapa Soja, 2005. p. 84-85. (Embrapa Soja. Documentos, 257). Organizado por Odilon Ferreira Saraiva, Janete Lasso Ortiz, Simone Ery Grosskopf.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 623 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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