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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
04/10/2011 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SALLES, H. O.; VASCONCELOS, I. M.; SANTOS, L. F. L.; OLIVEIRA, H. D.; COSTA, P. P. C.; NASCIMENTO, N. R. F.; SANTOS, C. F.; SOUSA, D. F.; JORGE, A. R. C.; MENEZES, D. B.; MONTEIRO, H. S. A.; GONDIM, D. M. F.; OLIVEIRA, J. T. A. |
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Afiliação: |
HEVILA OLIVEIRA SALLES FIGUEIREDO, CNPC; Universidade Federal do Ceará (UFC), Fortaleza, CE.; Universidade Estadual do Ceará (UECe) - Fortaleza, CE.; UECe; UECe; UECe; UECe; UFC, Fortaleza, CE.; UFC; UFC; UFC; UFC; UFC. |
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Título: |
Towards a better understanding of Ipomoea asarifolia toxicity: evidence of the involvement of a leaf lectin. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Toxicon, v. 58, p. 502-508, 2011. |
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DOI: |
10.1016/j.toxicon.2011.08.011 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Natural intoxication of livestock by ingestion of Ipomoea asarifolia leaves has been reported to occur widely in Brazil. Previous studies carried out by our research group provided strong evidence that a lectin could be involved with the toxic properties of I. asarifolia.To reinforce this hypothesis, a lectin-enriched fraction (LEF) was isolated from I. asarifolia leaves and its toxic effects were assessed. Leaves of I. asarifolia were excised from plants growing widely in the ?eld, mechanically wounded and maintained in a chamber at 25 3 C for 72 h in the dark, under near 100% relative humidity. The leaf proteins were extracted, ammonium sulfate precipitated, chromatographed on DEAE-cellulose and Phenyl-Sepharose to produce LEF that under SDS?PAGE showed a molecular mass of 44.0 kDa and after N-terminal amino acid analysis a primary sequence composed of AGYTPVLDIGAEVLAAGEPY. The in vivo toxicity of LEF assessed by intraorbital injection in mice showed induced severe uncoordinated movements without death. LEF reduced the muscular contraction in a dose depend way and at 29.8 mg/mL (CE50) it produces 50% inhibition of contraction, suggesting that LEF blunts autonomic neurotransmission. Isolated rat kidneys were perfused with LEF and no effects on the perfusion pressure or renal vascular resistance were observed, but urinary ?ow and glomerular ?ltration rate increased. Moreover, the percentage of tubular transport of Naþ,Kþ and Cl decreased. Histological examination of the kidneys perfused with LEF exhibited little alterations. These toxic effects observed above were concomitant with the increase of LEF hemag-glutination activity, which strongly suggest that one of the toxic principles of I. asarifolia is a lectin present in its leaves. MenosAbstract: Natural intoxication of livestock by ingestion of Ipomoea asarifolia leaves has been reported to occur widely in Brazil. Previous studies carried out by our research group provided strong evidence that a lectin could be involved with the toxic properties of I. asarifolia.To reinforce this hypothesis, a lectin-enriched fraction (LEF) was isolated from I. asarifolia leaves and its toxic effects were assessed. Leaves of I. asarifolia were excised from plants growing widely in the ?eld, mechanically wounded and maintained in a chamber at 25 3 C for 72 h in the dark, under near 100% relative humidity. The leaf proteins were extracted, ammonium sulfate precipitated, chromatographed on DEAE-cellulose and Phenyl-Sepharose to produce LEF that under SDS?PAGE showed a molecular mass of 44.0 kDa and after N-terminal amino acid analysis a primary sequence composed of AGYTPVLDIGAEVLAAGEPY. The in vivo toxicity of LEF assessed by intraorbital injection in mice showed induced severe uncoordinated movements without death. LEF reduced the muscular contraction in a dose depend way and at 29.8 mg/mL (CE50) it produces 50% inhibition of contraction, suggesting that LEF blunts autonomic neurotransmission. Isolated rat kidneys were perfused with LEF and no effects on the perfusion pressure or renal vascular resistance were observed, but urinary ?ow and glomerular ?ltration rate increased. Moreover, the percentage of tubular transport of Naþ,Kþ and Cl decreased. Histological examination o... Mostrar Tudo |
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Palavras-Chave: |
Ipomoea asarifolia; Lectin; Sulato de amônia. |
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Thesagro: |
Erva daninha; Lectina; Planta tóxica; Salsa; Toxidez. |
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Thesaurus Nal: |
toxicity. |
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Categoria do assunto: |
-- |
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Marc: |
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024 7 $a10.1016/j.toxicon.2011.08.011$2DOI
100 1 $aSALLES, H. O.
245 $aTowards a better understanding of Ipomoea asarifolia toxicity$bevidence of the involvement of a leaf lectin.$h[electronic resource]
260 $c2011
520 $aAbstract: Natural intoxication of livestock by ingestion of Ipomoea asarifolia leaves has been reported to occur widely in Brazil. Previous studies carried out by our research group provided strong evidence that a lectin could be involved with the toxic properties of I. asarifolia.To reinforce this hypothesis, a lectin-enriched fraction (LEF) was isolated from I. asarifolia leaves and its toxic effects were assessed. Leaves of I. asarifolia were excised from plants growing widely in the ?eld, mechanically wounded and maintained in a chamber at 25 3 C for 72 h in the dark, under near 100% relative humidity. The leaf proteins were extracted, ammonium sulfate precipitated, chromatographed on DEAE-cellulose and Phenyl-Sepharose to produce LEF that under SDS?PAGE showed a molecular mass of 44.0 kDa and after N-terminal amino acid analysis a primary sequence composed of AGYTPVLDIGAEVLAAGEPY. The in vivo toxicity of LEF assessed by intraorbital injection in mice showed induced severe uncoordinated movements without death. LEF reduced the muscular contraction in a dose depend way and at 29.8 mg/mL (CE50) it produces 50% inhibition of contraction, suggesting that LEF blunts autonomic neurotransmission. Isolated rat kidneys were perfused with LEF and no effects on the perfusion pressure or renal vascular resistance were observed, but urinary ?ow and glomerular ?ltration rate increased. Moreover, the percentage of tubular transport of Naþ,Kþ and Cl decreased. Histological examination of the kidneys perfused with LEF exhibited little alterations. These toxic effects observed above were concomitant with the increase of LEF hemag-glutination activity, which strongly suggest that one of the toxic principles of I. asarifolia is a lectin present in its leaves.
650 $atoxicity
650 $aErva daninha
650 $aLectina
650 $aPlanta tóxica
650 $aSalsa
650 $aToxidez
653 $aIpomoea asarifolia
653 $aLectin
653 $aSulato de amônia
700 1 $aVASCONCELOS, I. M.
700 1 $aSANTOS, L. F. L.
700 1 $aOLIVEIRA, H. D.
700 1 $aCOSTA, P. P. C.
700 1 $aNASCIMENTO, N. R. F.
700 1 $aSANTOS, C. F.
700 1 $aSOUSA, D. F.
700 1 $aJORGE, A. R. C.
700 1 $aMENEZES, D. B.
700 1 $aMONTEIRO, H. S. A.
700 1 $aGONDIM, D. M. F.
700 1 $aOLIVEIRA, J. T. A.
773 $tToxicon$gv. 58, p. 502-508, 2011.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 241 | |
| 66. |  | LEITE, E. R.; ARAUJO FILHO, J. A. de; MESQUITA, R. C. M.; CRISPIM, S. M. A. Estudo da dieta de caprinos em bancos de proteína no semi-árido do Ceará. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 25., 1988, Viçosa, MG. Anais... Viçosa, MG: Sociedade Brasileira de Zootecnia, 1988. p. 154.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 69. | | CARVALHO, F. C. de; ARAUJO FILHO, J. A. de; REGO, M. C. do; TELES, F. F. Flutuações dos níveis dos carboidratos de reserva disponíveis nas raízes e no caule do marmeleiro (Croton sonderianus Muell. Arg.). Revista Brasileira de Zootecnia, Viçosa, MG, v. 27, n. 4, p. 670-675, 1998.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 70. | | GADELHA, J. A.; ARAUJO FILHO, J. A. de; SOUZA, P. Z.; REGO, M. C. Flutuações da produção e composição da vegetação herbácea de caatinga nativa e manipulada sob pastejo de caprinos e ovinos. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 24., 1987, Brasília, DF. Anais... Brasília, DF: Sociedade Brasileira de Zootecnia, 1987. p. 255.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 73. | | ARAUJO FILHO, J. A. de; GADELHA, J. A.; SOUSA, P. Z. de. Cattle and goat grazing on the herbaceous cover of a lowered Caatinga. In: INTERNATIONAL CONFERENCE ON GOATS, 4., 1987, Brasília, DF. Proceedings... Brasília, DF: EMBRAPA-DDT, 1987. v. 2, p. 1431. Resumo 184| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 75. | | ARAUJO FILHO, J. A. de; CARVALHO, F. C. de; GARCIA, R.; SOUSA, R. A. de. Efeitos da manipulação da vegetação lenhosa sobre a produção e compartimentalização da fitomassa pastável de uma caatinga sucessional. Revista Brasileira de Zootecnia, Viçosa, MG, v. 31, n. 1, p. 11-19, 2002.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 241 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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