| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria de Alimentos. Para informações adicionais entre em contato com ctaa.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
|
Data corrente: |
11/01/2017 |
|
Data da última atualização: |
11/01/2017 |
|
Tipo da produção científica: |
Artigo em Anais de Congresso |
|
Autoria: |
NASCIMENTO, M. R. F. N.; SOUZA, V. F. de; MARINHO, A. F.; ASCHERI, J. L. R.; MALEIRO, C. H. de. |
|
Afiliação: |
Maria Rosa Figueiredo Nascimento, UFRRJ; Valéria França de Souza, UFRRJ; Angleson Figueira Marinho, UFRRJ; JOSE LUIS RAMIREZ ASCHERI, CTAA; Cristiane Hess de Azevedo Meleiro, UFRRJ. |
|
Título: |
Composto bioativo de caroço de abacate (Persea Gratissima, Gaertner F.). |
|
Ano de publicação: |
2016 |
|
Fonte/Imprenta: |
In: ENCONTRO AMAZÔNICO DE AGRÁRIAS, 8., 2016, Belém, PA. Recursos hídricos: uso sustentável e sua importância na agropecuária: anais. Belém, PA: [UFRA], 2016. p. 143-148. |
|
Idioma: |
Português |
|
Thesagro: |
Composto Fenólico; Farinha; Fruto; Impacto Ambiental; Valor Nutritivo. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 00754nam a2200205 a 4500
001 2060468
005 2017-01-11
008 2016 bl uuuu u00u1 u #d
100 1 $aNASCIMENTO, M. R. F. N.
245 $aComposto bioativo de caroço de abacate (Persea Gratissima, Gaertner F.).$h[electronic resource]
260 $aIn: ENCONTRO AMAZÔNICO DE AGRÁRIAS, 8., 2016, Belém, PA. Recursos hídricos: uso sustentável e sua importância na agropecuária: anais. Belém, PA: [UFRA], 2016. p. 143-148.$c2016
650 $aComposto Fenólico
650 $aFarinha
650 $aFruto
650 $aImpacto Ambiental
650 $aValor Nutritivo
700 1 $aSOUZA, V. F. de
700 1 $aMARINHO, A. F.
700 1 $aASCHERI, J. L. R.
700 1 $aMALEIRO, C. H. de
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agroindústria de Alimentos (CTAA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Hortaliças; Embrapa Instrumentação. |
|
Data corrente: |
18/11/2021 |
|
Data da última atualização: |
23/01/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
B - 2 |
|
Autoria: |
PETRÔNIO, M. S.; BARROS-ALEXANDRINO, T. T.; LIMA, A. M. F.; ASSIS, O. B. G. de; INOUE-NAGATA, A. K.; NAKASU, E. Y. T.; TIERA, M. J.; PILON, L. |
|
Afiliação: |
ODILIO BENEDITO GARRIDO DE ASSIS, CNPDIA; ALICE KAZUKO INOUE NAGATA, CNPH; ERICH YUKIO TEMPEL NAKASU, CNPH; LUCIMEIRE PILON, CNPH. |
|
Título: |
Physicochemical and toxicity investigation of chitosan-based dsRNA nanocarrier formation. |
|
Ano de publicação: |
2022 |
|
Fonte/Imprenta: |
Biointerface Research in Applied Chemistry, vol. 12, n. 4, 2022. |
|
Páginas: |
5266 - 5279 |
|
DOI: |
https://doi.org/10.33263/BRIAC124.52665279 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Technologies involving the use of double-stranded RNA (dsRNA) to elicit RNA interference (RNAi) in pest control have emerged as an alternative to traditional pesticides. RNAi can mediate natural cell protection being a promising tool to provide prompt responses in plant defense against pathogens. The present study is focused on the physicochemical characterization of formed dsRNAloaded nanoparticles as a result of chitosan-dsRNA ionic interactions. Additionally, a preliminary investigation was conducted of the in-vitro toxicity of loaded nanoparticles in lettuce and human red blood cells. dsRNA molecules, homologous to partial phytopathogenic tomato mosaic virus (ToMV) sequence, were used as a model. The main groups involved in the chitosan-dsRNA ionic coupling were identified by Fourier-transform infrared spectroscopy, and the stability of formed nanoparticles was accessed by dynamic light scattering, electrophoresis, and thermal analyses. The chitosan showed a higher ability to bind to dsRNA at low charge ratios (N/P = 1), ruled by positively charged chitosan methyl groups and negatively charged phosphate groups from the RNA backbone, resulting in small nanoparticles (73.25 nm size) at low polydispersity (0.25). The toxic assays of these particles, on lettuce seeds and in human erythrocytes, revealed very low toxicity demonstrating their safety as a platform, thereby holding potential use as biodefensive for crop protection. |
|
Palavras-Chave: |
Chitosan nanocarrier; DsRNA phytotoxicity assay; Tomato mosaic virus (ToMV). |
|
Thesaurus NAL: |
Double-stranded RNA; Lettuce; RNA interference; Tomato mosaic virus. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/232647/1/P-Physicochemical-and-Toxicity-Investigation-of-Chitosanbased-dsRNA-Nanocarrier-Formation.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227886/1/20695837124.526652791.pdf
|
|
Marc: |
LEADER 02428naa a2200313 a 4500
001 2141015
005 2024-01-23
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.33263/BRIAC124.52665279$2DOI
100 1 $aPETRÔNIO, M. S.
245 $aPhysicochemical and toxicity investigation of chitosan-based dsRNA nanocarrier formation.$h[electronic resource]
260 $c2022
300 $a5266 - 5279
520 $aTechnologies involving the use of double-stranded RNA (dsRNA) to elicit RNA interference (RNAi) in pest control have emerged as an alternative to traditional pesticides. RNAi can mediate natural cell protection being a promising tool to provide prompt responses in plant defense against pathogens. The present study is focused on the physicochemical characterization of formed dsRNAloaded nanoparticles as a result of chitosan-dsRNA ionic interactions. Additionally, a preliminary investigation was conducted of the in-vitro toxicity of loaded nanoparticles in lettuce and human red blood cells. dsRNA molecules, homologous to partial phytopathogenic tomato mosaic virus (ToMV) sequence, were used as a model. The main groups involved in the chitosan-dsRNA ionic coupling were identified by Fourier-transform infrared spectroscopy, and the stability of formed nanoparticles was accessed by dynamic light scattering, electrophoresis, and thermal analyses. The chitosan showed a higher ability to bind to dsRNA at low charge ratios (N/P = 1), ruled by positively charged chitosan methyl groups and negatively charged phosphate groups from the RNA backbone, resulting in small nanoparticles (73.25 nm size) at low polydispersity (0.25). The toxic assays of these particles, on lettuce seeds and in human erythrocytes, revealed very low toxicity demonstrating their safety as a platform, thereby holding potential use as biodefensive for crop protection.
650 $aDouble-stranded RNA
650 $aLettuce
650 $aRNA interference
650 $aTomato mosaic virus
653 $aChitosan nanocarrier
653 $aDsRNA phytotoxicity assay
653 $aTomato mosaic virus (ToMV)
700 1 $aBARROS-ALEXANDRINO, T. T.
700 1 $aLIMA, A. M. F.
700 1 $aASSIS, O. B. G. de
700 1 $aINOUE-NAGATA, A. K.
700 1 $aNAKASU, E. Y. T.
700 1 $aTIERA, M. J.
700 1 $aPILON, L.
773 $tBiointerface Research in Applied Chemistry, vol. 12$gn. 4, 2022.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Instrumentação (CNPDIA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
