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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
05/01/2012 |
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Data da última atualização: |
03/04/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
KULCHESKI, F. R.; OLIVEIRA, L. F. V.; MOLINA, L. G.; ALMERAO, M. P.; RODRIGUES, F. A.; MARCOLINO, J.; BARBOSA, J. F.; STOLF-MOREIRA, R.; NEPOMUCENO, A. L.; MARCELINO-GUIMARÃES, F. C.; ABDELNOOR, R. V.; NASCIMENTO, L. C.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; MARGIS, R. |
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Afiliação: |
FRANCELI R. KULCHESKI, UFRGS; LUIZ F. V. DE OLIVEIRA, UFRGS; LORRAYNE, G. MOLINA, UFRGS; MAURÍCIO P. ALMERÃO, UFRGS; FABIANA A. RODRIGUES, CNPSo; JULIANA MARCOLINO, CNPSo; JOICE F. BARBOSA, CNPSo; RENATA STOLF MOREIRA, CNPSo; ALEXANDRE LIMA NEPOMUCENO, SRI; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO; LEANDRO C. NASCIMENTO, UNICAMP; MARCELO F. CARAZZOLLE, UNICAMP; GONÇALO A. G. PEREIRA, UNICAMP; ROGÉRIO MARGIS, UFRGS. |
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Título: |
Identification of novel soybean microRNAs involved in abiotic and biotic stresses. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
BMC Genomics, v. 12, n. 307, jun. 2011. |
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Páginas: |
17 p. |
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DOI: |
10.1186/1471-2164-12-307 |
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Idioma: |
Português |
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Conteúdo: |
Background: Small RNAs (19-24 nt) are key regulators of gene expression that guide both transcriptional and posttranscriptional silencing mechanisms in eukaryotes. Current studies have demonstrated that microRNAs (miRNAs) act in several plant pathways associated with tissue proliferation, differentiation, and development and in response to abiotic and biotic stresses. In order to identify new miRNAs in soybean and to verify those that are possibly water deficit and rust-stress regulated, eight libraries of small RNAs were constructed and submitted to Solexa sequencing. Results: The libraries were developed from drought-sensitive and tolerant seedlings and rust-susceptible and resistant soybeans with or without stressors. Sequencing the library and subsequent analyses detected 256 miRNAs. From this total, we identified 24 families of novel miRNAs that had not been reported before, six families of conserved miRNAs that exist in other plants species, and 22 families previously reported in soybean. We also observed the presence of several isomiRNAs during our analyses. To validate novel miRNAs, we performed RT-qPCR across the eight different libraries. Among the 11 miRNAs analyzed, all showed different expression profiles during biotic and abiotic stresses to soybean. The majority of miRNAs were up-regulated during water deficit stress in the sensitive plants. However, for the tolerant genotype, most of the miRNAs were down regulated. The pattern of miRNAs expression was also different for the distinct genotypes submitted to the pathogen stress. Most miRNAs were down regulated during the fungus infection in the susceptible genotype; however, in the resistant genotype, most miRNAs did not vary during rust attack. A prediction of the putative targets was carried out for conserved and novel miRNAs families. Conclusions: Validation of our results with quantitative RT-qPCR revealed that Solexa sequencing is a powerful tool for miRNA discovery. The identification of differentially expressed plant miRNAs provides molecular evidence for the possible involvement of miRNAs in the process of water deficit- and rust-stress responses. MenosBackground: Small RNAs (19-24 nt) are key regulators of gene expression that guide both transcriptional and posttranscriptional silencing mechanisms in eukaryotes. Current studies have demonstrated that microRNAs (miRNAs) act in several plant pathways associated with tissue proliferation, differentiation, and development and in response to abiotic and biotic stresses. In order to identify new miRNAs in soybean and to verify those that are possibly water deficit and rust-stress regulated, eight libraries of small RNAs were constructed and submitted to Solexa sequencing. Results: The libraries were developed from drought-sensitive and tolerant seedlings and rust-susceptible and resistant soybeans with or without stressors. Sequencing the library and subsequent analyses detected 256 miRNAs. From this total, we identified 24 families of novel miRNAs that had not been reported before, six families of conserved miRNAs that exist in other plants species, and 22 families previously reported in soybean. We also observed the presence of several isomiRNAs during our analyses. To validate novel miRNAs, we performed RT-qPCR across the eight different libraries. Among the 11 miRNAs analyzed, all showed different expression profiles during biotic and abiotic stresses to soybean. The majority of miRNAs were up-regulated during water deficit stress in the sensitive plants. However, for the tolerant genotype, most of the miRNAs were down regulated. The pattern of miRNAs expression was also di... Mostrar Tudo |
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Thesagro: |
Deficiência hídrica; Gene marcador; Soja. |
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Thesaurus Nal: |
Gene expression; Soil water deficit; Soybeans. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/51756/1/BMC-2011.pdf
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Marc: |
LEADER 03216naa a2200385 a 4500
001 1911802
005 2013-04-03
008 2011 bl uuuu u00u1 u #d
024 7 $a10.1186/1471-2164-12-307$2DOI
100 1 $aKULCHESKI, F. R.
245 $aIdentification of novel soybean microRNAs involved in abiotic and biotic stresses.
260 $c2011
300 $a17 p.
520 $aBackground: Small RNAs (19-24 nt) are key regulators of gene expression that guide both transcriptional and posttranscriptional silencing mechanisms in eukaryotes. Current studies have demonstrated that microRNAs (miRNAs) act in several plant pathways associated with tissue proliferation, differentiation, and development and in response to abiotic and biotic stresses. In order to identify new miRNAs in soybean and to verify those that are possibly water deficit and rust-stress regulated, eight libraries of small RNAs were constructed and submitted to Solexa sequencing. Results: The libraries were developed from drought-sensitive and tolerant seedlings and rust-susceptible and resistant soybeans with or without stressors. Sequencing the library and subsequent analyses detected 256 miRNAs. From this total, we identified 24 families of novel miRNAs that had not been reported before, six families of conserved miRNAs that exist in other plants species, and 22 families previously reported in soybean. We also observed the presence of several isomiRNAs during our analyses. To validate novel miRNAs, we performed RT-qPCR across the eight different libraries. Among the 11 miRNAs analyzed, all showed different expression profiles during biotic and abiotic stresses to soybean. The majority of miRNAs were up-regulated during water deficit stress in the sensitive plants. However, for the tolerant genotype, most of the miRNAs were down regulated. The pattern of miRNAs expression was also different for the distinct genotypes submitted to the pathogen stress. Most miRNAs were down regulated during the fungus infection in the susceptible genotype; however, in the resistant genotype, most miRNAs did not vary during rust attack. A prediction of the putative targets was carried out for conserved and novel miRNAs families. Conclusions: Validation of our results with quantitative RT-qPCR revealed that Solexa sequencing is a powerful tool for miRNA discovery. The identification of differentially expressed plant miRNAs provides molecular evidence for the possible involvement of miRNAs in the process of water deficit- and rust-stress responses.
650 $aGene expression
650 $aSoil water deficit
650 $aSoybeans
650 $aDeficiência hídrica
650 $aGene marcador
650 $aSoja
700 1 $aOLIVEIRA, L. F. V.
700 1 $aMOLINA, L. G.
700 1 $aALMERAO, M. P.
700 1 $aRODRIGUES, F. A.
700 1 $aMARCOLINO, J.
700 1 $aBARBOSA, J. F.
700 1 $aSTOLF-MOREIRA, R.
700 1 $aNEPOMUCENO, A. L.
700 1 $aMARCELINO-GUIMARÃES, F. C.
700 1 $aABDELNOOR, R. V.
700 1 $aNASCIMENTO, L. C.
700 1 $aCARAZZOLLE, M. F.
700 1 $aPEREIRA, G. A. G.
700 1 $aMARGIS, R.
773 $tBMC Genomics$gv. 12, n. 307, jun. 2011.
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Registro original: |
Embrapa Soja (CNPSO) |
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Biblioteca |
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| Registros recuperados : 49 | |
| 41. |  | RODRIGUES, F. A.; MARCOLINO-GOMES, J.; CARVALHO, J. de F. C.; NASCIMENTO, L. C. do; NEUMAIER, N.; FARIAS, J. R. B.; CARAZZOLLE, M. F.; MARCELINO, F. C.; NEPOMUCENO, A. L. Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit. Genetics and Molecular Biology, Ribeirão Preto, v. 35, n. 1, suppl., p. 304-314, May 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Soja. |
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| 42. | | KIDO, É. A.; MARCELINO, F. C.; NASCIMENTO, L. C. do; SILVA, A. B. da; CARAZZOLLE, M. F.; BINNECK, E.; STOLF, R.; BARBOSA, J. F.; NEPOMUCENO, A. L.; JÚNIOR, T. C.; PEREIRA, G. A. G.; ABDELNOOR, R. V. Transcript analysis of soybean plants in response to Asian rust infection. In: WORLD SOYBEAN RESEARCH CONFERENCE, 8., 2009, Beijing. Developing a global soy blueprint for a safe secure and sustainable supply: abstracts. Beijing: Chinese Academy of Agricultural Sciences: Institute of Crop Science, 2009. p. 145, ref. P376. WSRC 2009. Editado por Lijuan Qiu, Rongxia Guan, Jian Jin, Qijan Song, Shuntang Guo, Wenbin Li, Yuanchao Wang, Tianfu Han, Xiaobing Liu, Deyue Yu, Lianzhou Jiang, Deliang Peng.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 43. | | MARCOLINO, J.; RODRIGUES, F. A.; AMARAL, L. C.; NAKAYAMA, T. J.; PEREIRA, R. M.; NASCIMENTO, L. C. do; BINNECK, E.; OLIVEIRA, M. C. N. de; MARCELINO, F. C.; ABDELNOOR, R. V.; NEUMAIER, N.; FARIAS, J. R. B.; NEPOMUCENO, A. L. Transcription factors expressed in soybean roots in response to water déficit. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 193-194.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 44. | | CARVALHO, M. C. C. G. de; NASCIMENTO, L. C.; POLIZEL-PODANOSQUI, A. M.; ROCHA, C. S.; DARBEN, L. M.; LOPES-CAITAR, V. S.; CARAZZOLLE, M. F.; OLIVEIRA, M. L. C. S.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. The transcriptome interactions between Phakopsora pachykopsora pachyrhizi-soybean. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 47.; SIMPÓSIO BRASILEIRO DE MOFO BRANCO, 2014, Londrina. Desafios futuros: anais. Londrina: SBF, 2014. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 45. | | CARVALHO, M. C. C. G.; LOPES, V. S.; NASCIMENTO, L. S.; NASCIMENTO, L. C.; CARAZZOLLE, M. F.; OLIVEIRA, M. L. C. S.; BINNECK, E.; ABDELNOOR, R. V.; MARCELINO, F. C. Wide transcriptome interaction from resistance and tolerant soybean genotypes under Phakospsora pachryrhi infection revelad by LCM and RNAseq. In: BRAZILIAN SYMPOSIUM ON PLANT MOLECULAR BIOLOGY, 3., 2011, Ilhéus. [Abstracts...]. [Ribeirão Preto]: SBG, 2011. 1 p. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 46. | | BENKO-ISEPPON, A. M.; BEZERRA-NETO, J. P.; PANDOLFI, V.; WANDERLEY-NOGUEIRA, A. C.; BELARMINO L. C.; FERREIRA NETO, J. R. C.; SOARES-CAVALCANTI, N. M.; BARBOSA-AMORIM, L. L.; CALSA-JR, T.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C.; NEPOMUCENO, A. L.; NASCIMENTO, L. C.; CARAZZOLLE, M. F.; PEREIRA, G. G.; KIDO, E. A. Drought and salinity stress response in legume crops. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 4., Bento Gonçalves, 2013. Resumo... [Ribeirão Preto]: Sociedade Brasileira de Genética, 2013. p. 5. Palestra.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 47. | | ROMERO, C. C. T.; STOLF-MOREIRA, R.; BRITO JÚNIOR, S. L.; CATELLI, L. L.; PEREIRA, R. M.; LINO, E. J.; SILLA, P. R.; NASCIMENTO, L. S. do; NASCIMENTO, L. C. do; CARAZZOLLE, M. F.; BINNECK, E.; MARCELINO, F. C.; ABDELNOOR, R. V. Genome-wide expression analysis of soybean plants under an incompatible interaction with the fungus Uromyces appendiculatus. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 219-220.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 48. | | KULCHESKI, F. R.; OLIVEIRA, L. F. V.; MOLINA, L. G.; ALMERAO, M. P.; RODRIGUES, F. A.; MARCOLINO, J.; BARBOSA, J. F.; STOLF-MOREIRA, R.; NEPOMUCENO, A. L.; MARCELINO-GUIMARÃES, F. C.; ABDELNOOR, R. V.; NASCIMENTO, L. C.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; MARGIS, R. Identification of novel soybean microRNAs involved in abiotic and biotic stresses. BMC Genomics, v. 12, n. 307, jun. 2011. 17 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 49. | | KULCHESKI, F. R.; OLIVEIRA, L. F. V.; MOLINA, L. G.; ALMERÃO, M.; RODRIGUES, F. A.; MARCOLINO, J.; BARBOSA, J. F.; STOLF-MOREIRA, R.; NEPOMUCENO, A. L.; MARCELINO, F. C.; ABDELNOOR, R. V.; NASCIMENTO, L. C.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; MARGIS, R. Identification of novel soybean microRNAs involved in abiotic and biotic stresses. In: BRAZILIAN SYMPOSIUM ON PLANT MOLECULAR BIOLOGY, 3., 2011, Ilhéus. [Abstracts...]. [Ribeirão Preto]: SBG, 2011. 1 p. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 49 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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